Biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortium

Background: Biodegradation of hydrocarbon compounds is a great environmental concern due to their toxic nature and ubiquitous occurrence. In this study, biodegradation potential of oily soils was investigated in an oil field using indigenous bacterial consortium. Methods: The bacterial strains present in the contaminated and non-contaminated soils were identified via DNA extraction using 16S rDNA gene sequencing during six months. Furthermore, total petroleum hydrocarbons (TPH) were removed from oil-contaminated soils. The TPH values were determined using a gas chromatograph equipped with a flame ionization detector (GC-FID). Results: The bacterial consortium identified in oil-contaminated soils (case) belonged to the families Halomonadaceae (91.5%) and Bacillaceae (8.5%), which was significantly different from those identified in non-contaminated soils (control) belonging to the families Enterobacteriaceae (84.6%), Paenibacillaceae (6%), and Bacillaceae (9.4%). It was revealed that the diversity of bacterial strains was less in oil-contaminated soils and varied significantly between case and control samples. Indigenous bacterial consortium was used in oil-contaminated soils without need for amplification of heterogeneous bacteria and the results showed that the identified bacterial strains could be introduced as a sufficient consortium for biodegradation of oil-contaminated soils with similar texture, which is one of the innovative aspects of this research. Conclusion: An oil-contaminated soil sample with TPH concentration of 1640 mg/kg was subjected to bioremediation during 6 months using indigenous bacterial consortium and a TPH removal efficiency of 28.1% was obtained.

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Bioremediation of Petroleum Hydrocarbons in Seawater: Prospects of Using Lyophilized Native Hydrocarbon-Degrading Bacteria

Microorganisms ◽

10.3390/microorganisms9112285 ◽

2021 ◽

Vol 9 (11) ◽

pp. 2285

Author(s):

Rafaela Perdigão ◽

C. Marisa R. Almeida ◽

Catarina Magalhães ◽

Sandra Ramos ◽

Ana L. Carolas ◽

...

Keyword(s):

Petroleum Hydrocarbons ◽

Carbon Sources ◽

Rhodococcus Erythropolis ◽

Water Layer ◽

Degradation Process ◽

Bacterial Consortium ◽

Total Petroleum Hydrocarbons ◽

Natural Seawater ◽

Bacterial Strains ◽

Degrading Bacteria

This work aimed to develop a bioremediation product of lyophilized native bacteria to respond to marine oil spills. Three oil-degrading bacterial strains (two strains of Rhodococcus erythropolis and one Pseudomonas sp.), isolated from the NW Portuguese coast, were selected for lyophilization after biomass growth optimization (tested with alternative carbon sources). Results indicated that the bacterial strains remained viable after the lyophilization process, without losing their biodegradation potential. The biomass/petroleum ratio was optimized, and the bioremediation efficiency of the lyophilized bacterial consortium was tested in microcosms with natural seawater and petroleum. An acceleration of the natural oil degradation process was observed, with an increased abundance of oil-degraders after 24 h, an emulsion of the oil/water layer after 7 days, and an increased removal of total petroleum hydrocarbons (47%) after 15 days. This study provides an insight into the formulation and optimization of lyophilized bacterial agents for application in autochthonous oil bioremediation.

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Development, optimization, validation and application of faster gas chromatography – flame ionization detector method for the analysis of total petroleum hydrocarbons in contaminated soils

Journal of Chromatography A ◽

10.1016/j.chroma.2015.10.003 ◽

2015 ◽

Vol 1425 ◽

pp. 240-248 ◽

Cited By ~ 5

Author(s):

Abdulrazaq Zubair ◽

Michael Pappoe ◽

Lesley A. James ◽

Kelly Hawboldt

Keyword(s):

Gas Chromatography ◽

Contaminated Soils ◽

Petroleum Hydrocarbons ◽

Flame Ionization Detector ◽

Total Petroleum Hydrocarbons ◽

Ionization Detector ◽

Flame Ionization ◽

Detector Method

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Total Petroleum Hydrocarbons Categories Concentration in Soils Within the Vicinity Housing Heavy-Duty Diesel Generators in Three Universities in Port Harcourt South-South Nigeria

Earth & Environmental Science Research & Reviews ◽

10.33140/eesrr.04.01.07 ◽

2021 ◽

Vol 4 (1) ◽

Keyword(s):

Petroleum Hydrocarbons ◽

Health Hazard ◽

Soxhlet Extraction ◽

Soil Samples ◽

Total Petroleum Hydrocarbons ◽

Heavy Duty ◽

Flame Ionization ◽

Port Harcourt ◽

Diesel Generators ◽

Heavy Duty Diesel

Total petroleum hydrocarbons pollution of soil samples randomly collected from three Nigeria Universities in Port Harcourt due to the use of heavy-duty diesel generators was studied to ascertains the level of concentration of the different hydrocarbons’ categories. The soil samples were collected at two different depths of 0.00-0.50m and 0.50-1.00m. The Universities were Ignatius Ajuru University of Education (IAUE), Rivers State University (RSU) and University of Port Harcourt (UNIPORT). The different total petroleum hydrocarbons categories were Gasoline Range Organics (GRO), Diesel Range Organics (DRO) and Lube Oil Range. Soxhlet extraction method was used in extracting the samples and due column clean-up was performed for chromatographic analysis. Gas Chromatography-Flame Ionization Detector was used to determine the level of concentrations of the different categories of total petroleum hydrocarbons. The results showed that at 0.00-0.50m depth, IAUE was 4.42145, 945.4784, and 525.66919 mg/Kg for GRO, DRO and lube oil range respectively, RSU was not detected, 494.44799 and 458.6715 mg/Kg for GRO, DRO and lube oil range respectively and UNIPORT was 4.40920, 501.2246 and 467.71426 mg/Kg for GRO, DRO and lube oil range respectively. At 0.50-1.00m depth IAUE was 2.75132, 596.35126, and 311.84451 mg/Kg for GRO, DRO and lube oil range respectively, RSU was not detected, 298.06899 and 270.61619 mg/Kg for GRO, DRO and lube oil range respectively and UNIPORT was 2.77780, 301.74701 and 276.88684 mg/Kg for GRO, DRO and lube oil range respectively. The level of soil contamination Showed that GRO > DRO > lube oil range. The observation showed that hydrocarbon pollution decreased with increase in depth. The level of DRO and lube oil range in the studied areas exceeded the limit acceptable and therefore adequate steps should be taken to remedy the situation so that it will not pose any health hazard to the workers operating the heavy-duty generators.

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Efficacy of Cavern Isolates for Biodegradation of Synthetic Plastic

Letters in Organic Chemistry ◽

10.2174/1570178618666210602161857 ◽

2021 ◽

Vol 18 ◽

Author(s):

Jamila Tabassum ◽

Muhammad Luqman ◽

Fariha Hasan ◽

Fariha Arooj ◽

Saif Ur Rehman Kashif ◽

...

Keyword(s):

Environmental Concern ◽

Mechanical Damage ◽

Bacterial Consortium ◽

Alcaligenes Faecalis ◽

Bacterial Strains ◽

Ldpe Film ◽

Intermediate Metabolites ◽

Bacillus Sonorensis ◽

Physical Changes ◽

First Time

: Synthetic plastic waste management is a tenacious environmental concern at the global level. Although all types of synthetic plastics are a nuisance to the environment, however, versatility and one time use have made polyethylene (PE) a foremost environmental issue. The current study has investigated cavern bacterial strains isolated from PE samples from San Giovanni cave, Sardinia, Italy for their efficacy to biodegrade low-density polyethylene (LDPE) film. It was an initial effort to use cavern bacteria in plastic biodegradation studies. Chemical and physical changes in the composition of LDPE were studied by Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) after incubation with the bacterial consortium for two months. Collected cavern PE plastic samples were also studied for biodegradation after incubation in nutrient broth for two months. FTIR revealed obvious signs of degradation with the appearance of two new peaks of functional groups, nitriles (C≡N) and amines (N-H) in LDPE film, which are intermediate metabolites of β- oxidation pathway. An increase in various existing peaks of several intermediate metabolites including aldehydes, ketones, alcohols, and carboxylic acids, were also observed in experimental LDPE compared to control. Peaks of alkanes decreased significantly owing to cavern bacterial activity. SEM revealed biofilm formation on experimental LDPE surface with substantial mechanical damage. Similar signs of degradation were observed in the cavern PE samples. Four bacterial strains in the current consortium, including Bacillus sonorensis, Bacillus subtilis, Aneurinibacillus spp., and Alcaligenes faecalis are first time reported to be linked with biodegradation of plastics. The cavern bacteria under study have the potential to biodegrade LDPE.

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Effect of compost in phytoremediation of diesel-contaminated soils

Water Science & Technology ◽

10.2166/wst.2001.0102 ◽

2001 ◽

Vol 43 (2) ◽

pp. 291-295 ◽

Cited By ~ 26

Author(s):

J. Vouillamoz ◽

M. W. Milke

Keyword(s):

Moisture Content ◽

Contaminated Soil ◽

Contaminated Soils ◽

Petroleum Hydrocarbons ◽

Dilution Effect ◽

Screening Tests ◽

Total Petroleum Hydrocarbons ◽

Controlled Environment ◽

Grass Growth ◽

The Impact

The effect of compost on phytoremediation of diesel-contaminated soils was investigated using 130 small (200 g) containers in two screening tests. The experiments were conducted in a controlled environment using ryegrass from seed. Containers were destructively sampled at various times and analyzed for plant mass and total petroleum hydrocarbons. The results indicate that the presence of diesel reduces grass growth, and that compost helps reduced the impact of diesel on grass growth. The addition of compost helps increase diesel loss from the soils both with and without grass, though the addition of grass leads to lower diesel levels compared with controls. A second set of experiments indicates that the compost helps in phytoremediation of diesel-contaminated soil independent of the dilution effect that compost addition has. The results indicate that the compost addition allowed diesel loss down to 200 mg TPH/kg even though the compost would be expected to hold the diesel more tightly in the soil/compost mixture. The simplicity of the screening tests led to difficulties in controlling moisture content and germination rates. The conclusion of the research is that the tilling of compost into soils combined with grass seeding appears to be a valuable option for treating petroleum-contaminated soils.

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Total Petroleum Hydrocarbons (TPH) in the Soil of West Qurna-2 Oil Field Southern Iraq

International Journal of Marine Science ◽

10.5376/ijms.2017.07.0006 ◽

2017 ◽

Author(s):

Hamid T. Al-Saad ◽

Duha S. Karem ◽

Hamzah A. Kadhim

Keyword(s):

Petroleum Hydrocarbons ◽

Oil Field ◽

Total Petroleum Hydrocarbons ◽

Southern Iraq

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Biodegradation Potentials of Aspergillus sydowii and Fusarium lichenicola on Total Petroleum Hydrocarbon in an Oilfield Wastewater in Rivers State

Journal of Advances in Microbiology ◽

10.9734/jamb/2019/v19i330194 ◽

2020 ◽

pp. 1-7

Author(s):

Williams, Janet Olufunmilayo ◽

Aleruchi Owhonka

Keyword(s):

Mixed Culture ◽

Contaminated Soils ◽

Petroleum Hydrocarbons ◽

Petroleum Hydrocarbon ◽

Total Petroleum Hydrocarbon ◽

Total Petroleum Hydrocarbons ◽

Aspergillus Sydowii ◽

Significant Difference ◽

Set Up ◽

Rivers State

This study investigated the potential of Aspergillus sydowii and Fusarium lichenicola as mixed cultures in the biodegradation of Total Petroleum Hydrocarbons TPHs in oilfield wastewater. Oilfield wastewater was collected from an onshore oil producing platform and biodegradation of total petroleum hydrocarbons was investigated using standard methods. Fungi were isolated from oilfield wastewater contaminated soils obtained from the vicinity of the oil producing platform. Experimental control set-up and treatment with mixed culture of fungal isolates were periodically analyzed on days 7 and 21 intervals for total petroleum hydrocarbon degradation using Gas Chromatography (GC). The total amount of TPHs on day 1 recorded 381. 871 mg/l. The amount of TPHs on days 7 and 21 in the mixed culture of fungi was 108.975 mg/l and 21.105 mg/l respectively while TPHs in control was 342.891 mg/l and 240.749 mg/l respectively. There was a significant difference between the mixed culture and the control on days 7 and 21 at p≤0.05. The results therefore revealed actual and significant reduction of TPHs in the mixed culture. In addition, there was clearance of n-alkanes by the mixed culture. This suggests that fungi have great potentials in biodegradation of TPHs and in remediation of TPH contaminated environments.

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