Potential Use of Waste-to-Bioenergy By-Products in Bioremediation of Total Petroleum Hydrocarbons (TPH)-Contaminated Soils

The effect of compost on phytoremediation of diesel-contaminated soils was investigated using 130 small (200 g) containers in two screening tests. The experiments were conducted in a controlled environment using ryegrass from seed. Containers were destructively sampled at various times and analyzed for plant mass and total petroleum hydrocarbons. The results indicate that the presence of diesel reduces grass growth, and that compost helps reduced the impact of diesel on grass growth. The addition of compost helps increase diesel loss from the soils both with and without grass, though the addition of grass leads to lower diesel levels compared with controls. A second set of experiments indicates that the compost helps in phytoremediation of diesel-contaminated soil independent of the dilution effect that compost addition has. The results indicate that the compost addition allowed diesel loss down to 200 mg TPH/kg even though the compost would be expected to hold the diesel more tightly in the soil/compost mixture. The simplicity of the screening tests led to difficulties in controlling moisture content and germination rates. The conclusion of the research is that the tilling of compost into soils combined with grass seeding appears to be a valuable option for treating petroleum-contaminated soils.

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Biodegradation Potentials of Aspergillus sydowii and Fusarium lichenicola on Total Petroleum Hydrocarbon in an Oilfield Wastewater in Rivers State

Journal of Advances in Microbiology ◽

10.9734/jamb/2019/v19i330194 ◽

2020 ◽

pp. 1-7

Author(s):

Williams, Janet Olufunmilayo ◽

Aleruchi Owhonka

Keyword(s):

Mixed Culture ◽

Contaminated Soils ◽

Petroleum Hydrocarbons ◽

Petroleum Hydrocarbon ◽

Total Petroleum Hydrocarbon ◽

Total Petroleum Hydrocarbons ◽

Aspergillus Sydowii ◽

Significant Difference ◽

Set Up ◽

Rivers State

This study investigated the potential of Aspergillus sydowii and Fusarium lichenicola as mixed cultures in the biodegradation of Total Petroleum Hydrocarbons TPHs in oilfield wastewater. Oilfield wastewater was collected from an onshore oil producing platform and biodegradation of total petroleum hydrocarbons was investigated using standard methods. Fungi were isolated from oilfield wastewater contaminated soils obtained from the vicinity of the oil producing platform. Experimental control set-up and treatment with mixed culture of fungal isolates were periodically analyzed on days 7 and 21 intervals for total petroleum hydrocarbon degradation using Gas Chromatography (GC). The total amount of TPHs on day 1 recorded 381. 871 mg/l. The amount of TPHs on days 7 and 21 in the mixed culture of fungi was 108.975 mg/l and 21.105 mg/l respectively while TPHs in control was 342.891 mg/l and 240.749 mg/l respectively. There was a significant difference between the mixed culture and the control on days 7 and 21 at p≤0.05. The results therefore revealed actual and significant reduction of TPHs in the mixed culture. In addition, there was clearance of n-alkanes by the mixed culture. This suggests that fungi have great potentials in biodegradation of TPHs and in remediation of TPH contaminated environments.

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Biodegradation of total petroleum hydrocarbons (TPH) in highly contaminated soils by natural attenuation and bioaugmentation

Chemosphere ◽

10.1016/j.chemosphere.2019.06.111 ◽

2019 ◽

Vol 234 ◽

pp. 864-874 ◽

Cited By ~ 15

Author(s):

Marie Thérèse Bidja Abena ◽

Tongtong Li ◽

Muhammad Naeem Shah ◽

Weihong Zhong

Keyword(s):

Natural Attenuation ◽

Contaminated Soils ◽

Petroleum Hydrocarbons ◽

Total Petroleum Hydrocarbons

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The use of vinasse as an amendment to ex-situ bioremediation of soil and groundwater contaminated with diesel oil

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132009000400030 ◽

2009 ◽

Vol 52 (4) ◽

pp. 1043-1055 ◽

Cited By ~ 13

Author(s):

Adriano Pinto Mariano ◽

Sérgio Henrique Rezende Crivelaro ◽

Dejanira de Franceschi de Angelis ◽

Daniel Marcos Bonotto

Keyword(s):

Contaminated Soils ◽

Petroleum Hydrocarbons ◽

Microbial Population ◽

Diesel Oil ◽

Ex Situ ◽

Total Petroleum Hydrocarbons ◽

Co2 Production ◽

Soil Bioremediation ◽

Negative Effects ◽

Labile Carbon

This work investigated the possibility of using vinasse as an amendment in ex-situ bioremediation processes. Groundwater and soil samples were collected at petrol stations. The soil bioremediation was simulated in Bartha biometer flasks, used to measure the microbial CO2 production, during 48 days, where vinasse was added at a concentration of 33 mL.Kg-1of soil. Biodegradation efficiency was also measured by quantifying the total petroleum hydrocarbons (TPH) by gas chromatography. The groundwater bioremediation was carried out in laboratory experiments simulating aerated (bioreactors) and not aerated (BOD flasks) conditions. In both the cases, the concentration of vinasse was 5 % (v/v) and different physicochemical parameters were evaluated during 20 days. Although an increase in the soil fertility and microbial population were obtained with the vinasse, it demonstrated not to be adequate to enhance the bioremediation efficiency of diesel oil contaminated soils. The addition of the vinasse in the contaminated groundwaters had negative effects on the biodegradation of the hydrocarbons, since vinasse, as a labile carbon source, was preferentially consumed.

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Determination of total petroleum hydrocarbons in soil from different locations using infrared spectrophotometry and gas chromatography

Chemical Papers ◽

10.2478/s11696-012-0193-8 ◽

2012 ◽

Vol 66 (8) ◽

Cited By ~ 18

Author(s):

Paula Paíga ◽

Lurdes Mendes ◽

José Albergaria ◽

Cristina Delerue-Matos

Keyword(s):

Gas Chromatography ◽

Organic Matter ◽

Analytical Methods ◽

Contaminated Soils ◽

Petroleum Hydrocarbons ◽

Organic Matter Content ◽

Matter Content ◽

Total Petroleum Hydrocarbons ◽

Infrared Spectrometry ◽

Ir Analysis

AbstractTotal petroleum hydrocarbons (TPH) are important environmental contaminants which are toxic to human and environmental receptors. Several analytical methods have been used to quantify TPH levels in contaminated soils, specifically through infrared spectrometry (IR) and gas chromatography (GC). Despite being two of the most used techniques, some issues remain that have been inadequately studied: a) applicability of both techniques to soils contaminated with two distinct types of fuel (petrol and diesel), b) influence of the soil natural organic matter content on the results achieved by various analytical methods, and c) evaluation of the performance of both techniques in analyses of soils with different levels of contamination (presumably non-contaminated and potentially contaminated). The main objectives of this work were to answer these questions and to provide more complete information about the potentials and limitations of GC and IR techniques. The results led us to the following conclusions: a) IR analysis of soils contaminated with petrol is not suitable due to volatilisation losses, b) there is a significant influence of organic matter in IR analysis, and c) both techniques demonstrated the capacity to accurately quantify TPH in soils, irrespective of their contamination levels.

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Biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortium

Environmental Health Engineering and Management ◽

10.34172/ehem.2020.15 ◽

2020 ◽

Vol 7 (2) ◽

pp. 127-133

Author(s):

Yalda Basim ◽

Ghasemali Mohebali ◽

Sahand Jorfi ◽

Ramin Nabizadeh ◽

Mehdi Ahmadi Moghadam ◽

...

Keyword(s):

Contaminated Soils ◽

Petroleum Hydrocarbons ◽

Environmental Concern ◽

Bacterial Consortium ◽

Oil Field ◽

Total Petroleum Hydrocarbons ◽

Bacterial Strains ◽

Flame Ionization ◽

And Control ◽

Similar Texture

Background: Biodegradation of hydrocarbon compounds is a great environmental concern due to their toxic nature and ubiquitous occurrence. In this study, biodegradation potential of oily soils was investigated in an oil field using indigenous bacterial consortium. Methods: The bacterial strains present in the contaminated and non-contaminated soils were identified via DNA extraction using 16S rDNA gene sequencing during six months. Furthermore, total petroleum hydrocarbons (TPH) were removed from oil-contaminated soils. The TPH values were determined using a gas chromatograph equipped with a flame ionization detector (GC-FID). Results: The bacterial consortium identified in oil-contaminated soils (case) belonged to the families Halomonadaceae (91.5%) and Bacillaceae (8.5%), which was significantly different from those identified in non-contaminated soils (control) belonging to the families Enterobacteriaceae (84.6%), Paenibacillaceae (6%), and Bacillaceae (9.4%). It was revealed that the diversity of bacterial strains was less in oil-contaminated soils and varied significantly between case and control samples. Indigenous bacterial consortium was used in oil-contaminated soils without need for amplification of heterogeneous bacteria and the results showed that the identified bacterial strains could be introduced as a sufficient consortium for biodegradation of oil-contaminated soils with similar texture, which is one of the innovative aspects of this research. Conclusion: An oil-contaminated soil sample with TPH concentration of 1640 mg/kg was subjected to bioremediation during 6 months using indigenous bacterial consortium and a TPH removal efficiency of 28.1% was obtained.

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Bioremediation of Soil Contaminated with Diesel using Biopile system

Al-Khwarizmi Engineering Journal ◽

10.22153/https://doi.org/10.22153/kej.2018.12.009 ◽

2018 ◽

Vol 14 (3) ◽

pp. 48-56

Author(s):

Noor Mohsen Jabbar ◽

Estabriq Hasan Kadhim ◽

Alaa Kareem Mohammed

Keyword(s):

Contaminated Soil ◽

Contaminated Soils ◽

Petroleum Hydrocarbons ◽

Bacterial Culture ◽

Atmospheric Temperature ◽

Pilot Scale ◽

Ex Situ ◽

Total Petroleum Hydrocarbons ◽

Bacterial Inoculum ◽

Amended Soil

This study was focused on biotreatment of soil which polluted by petroleum compounds (Diesel) which caused serious environmental problems. One of the most effective and promising ways to treat diesel-contaminated soil is bioremediation. It is a choice that offers the potential to destroy harmful pollutants using biological activity. The capability of mixed bacterial culture was examined to remediate the diesel-contaminated soil in bio piling system. For fast ex-situ treatment of diesel-contaminated soils, the bio pile system was selected. Two pilot scale bio piles (25 kg soil each) were constructed containing soils contaminated with approximately 2140 mg/kg total petroleum hydrocarbons (TPHs). The amended soil: (contaminated soil with the addition of nutrients and bacterial inoculum), where the soil was mixed with 1.5% of sawdust, then supplied with the necessary nutrients and watered daily to provide conditions promoting microorganism growth. Unamended soil was prepared as a control (contaminated soil without addition). Both systems were equipped with oxygen to provide aerobic conditions, incubated at atmospheric temperature and weekly sampling within 35 days. Overall 75% of the total petroleum hydrocarbons were removed from the amended soil and 38 % of the control soil at the end of study period. The study concluded that ex-situ experiment (Bio pile) is a preferable, economical, and environmentally friendly procedure, thus representing a good option for the treatment of soil contaminated with diesel.

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Phytoremediation of hydrocarbon-contaminated soils with emphasis on the effect of petroleum hydrocarbons on the growth of plant species

Phytoprotection ◽

10.7202/000379ar ◽

2009 ◽

Vol 89 (1) ◽

pp. 21-29 ◽

Cited By ~ 41

Author(s):

Ravanbakhsh Shirdam ◽

Ali Daryabeigi Zand ◽

Gholamreza Nabi Bidhendi ◽

Nasser Mehrdadi

Keyword(s):

Plant Species ◽

Contaminated Soil ◽

Contaminated Soils ◽

Petroleum Hydrocarbons ◽

Petroleum Hydrocarbon ◽

Green Technology ◽

Hydrocarbon Contamination ◽

Total Petroleum Hydrocarbons ◽

Control Treatment ◽

Polluted Soils

To date, many developing countries such as Iran have almost completely abandoned the idea of decontaminating oil-polluted soils due to the high costs of conventional (physical/chemical) soil remediation methods. Phytoremediation is an emerging green technology that can become a promising solution to the problem of decontaminating hydrocarbon-polluted soils. Screening the capacity of native tolerant plant species to grow on aged, petroleum hydrocarbon-contaminated soils is a key factor for successful phytoremediation. This study investigated the effect of hydrocarbon pollution with an initial concentration of 40 000 ppm on growth characteristics of sorghum (Sorghum bicolor) and common flax (Linum usitatissumum). At the end of the experiment, soil samples in which plant species had grown well were analyzed for total petroleum hydrocarbons (TPHs) removal by GC-FID. Common flax was used for the first time in the history of phytoremediation of oil-contaminated soil. Both species showed promising remediation efficiency in highly contaminated soil; however, petroleum hydrocarbon contamination reduced the growth of the surveyed plants significantly. Sorghum and common flax reduced TPHs concentration by 9500 and 18500 mg kg‑1, respectively, compared with the control treatment.

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Surfactant Enhanced Bioremediation of Hydrocarbon Contaminated Soils Using Alkyl Polyglucoside

Asian Journal of Physical and Chemical Sciences ◽

10.9734/ajopacs/2021/v9i330138 ◽

2021 ◽

pp. 17-28

Author(s):

C. E. Ezekiel ◽

Leo C. Osuji ◽

M. C. Onojake

Keyword(s):

Contaminated Soils ◽

Petroleum Hydrocarbons ◽

Soil Samples ◽

Hydrocarbon Contamination ◽

Total Petroleum Hydrocarbons ◽

Alkyl Polyglycosides ◽

Enhanced Biodegradation ◽

Enhanced Bioremediation ◽

Reduction Percentage ◽

Alkyl Polyglucoside

Bioremediation is an efficient and environmentally friendly method for the degradation of petroleum hydrocarbons in contaminated soils. This study investigated the effects of biosurfactant alkyl polyglycosides (APG) on enhanced biodegradation of petroleum hydrocarbon contaminated soils. Three soil samples were contaminated with two different grades of crude oil (medium and Light). Alkyl polyglucoside was synthesised and subjected to FTIR for comfirmation of the product before it was applied in the remediation of contaminated soil. The alkyl polyglucoside is used as a treatment regime in the remediation of the hydrocarbon contamination in the three soil samples. Results of total petroleum hydrocarbons (TPH) before remediation with bio-surfactant showed that samples contaminated with medium crude for Eneka, Ozuoba and Rukpokwu were 15744.00 mg/kg, 11359.00 mg/kg and 11470.00 mg/kg respectively and after remediation reduced to 4276.00 mg/kg, 4265.00 mg/kg, and 3205.00 mg/kg, showing a reduction percentage of 72.84%, 62.44% and 72.05% respectively. Soil samples contaminated with light crude showed result of TPH of 11339.00 mg/kg, 10662 mg/kg and 10226 mg/kg and after remediation reduced to 2981 mg/kg, 3879 mg/kg, and 4245 mg/kg respectively showing a reduction percentage of 73.71%, 63.62 % and 58.49% respectively. The enhanced efficiency of the bio-surfactant at degrading total petroleum hydrocarbons was achieved as a result of the increased solubility thus improving the bioavailability of the hydrocarbons due to the action of the alkyl polyglucoside.

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Uso de Leersia hexandra (Poaceae) en la fitorremediación de suelos contaminados con petróleo fresco e intemperizado

Revista de Biología Tropical ◽

10.15517/rbt.v65i1.22967 ◽

2016 ◽

Vol 65 (1) ◽

pp. 21 ◽

Cited By ~ 3

Author(s):

Alfredo Arias Trinidad ◽

María Del Carmen Rivera Cruz ◽

Antonio Roldán Garrigós ◽

Lorenzo Armando Aceves Navarro ◽

Roberto Quintero-Lizaola ◽

...

Keyword(s):

Biomass Production ◽

Contaminated Soils ◽

Petroleum Hydrocarbons ◽

Plant Biomass ◽

Soxhlet Extraction ◽

Dry Weight ◽

Total Petroleum Hydrocarbons ◽

Control Treatment ◽

Experimental Conditions ◽

Leersia Hexandra

The oil industry has generated chronic oil spills and their accumulation in wetlands of the state of Tabasco, in Southeastern Mexico. Waterlogging is a factor that limits the use of remediation technologies because of its high cost and low levels of oil degradation. However, Leersia hexandra is a grass that grows in these contaminated areas with weathered oil. The aim of the study was to evaluate the bacteria density, plant biomass production and phytoremediation of L. hexandra in contaminated soil. For this, two experiments in plastic tunnel were performed with fresh (E1) and weathered petroleum (E2) under waterlogging experimental conditions. The E1 was based on eight doses: 6 000, 10 000, 30 000, 60 000, 90 000, 120 000, 150 000 and 180 000 mg.kg-1 dry basis (d. b.) of total petroleum hydrocarbons fresh (TPH-F), and the E2, that evaluated five doses: 14 173, 28 400, 50 598, 75 492 and 112 142 mg. kg-1 d. b. of total petroleum hydrocarbons weathered (TPH-W); a control treatment with 2 607 mg.kg-1 d. b. was used. Each experiment, with eight replicates per treatment, evaluated after three and six months: a) microbial density of total free-living nitrogen-fixing bacteria (NFB) of Azospirillum (AZP) and Azotobacter group (AZT), for viable count in serial plate; b) dry matter production (DMP), quantified gravimetrically as dry weight of L. hexandra; and c) the decontamination percentage of hydrocarbons (PDH) by Soxhlet extraction. In soil with TPH-F, the NFB, AZP y AZT populations were stimulated five times more than the control both at the three and six months; however, concentrations of 150 000 and 180 000 mg.kg-1 d. b. inhibited the bacterial density between 70 and 89 %. Likewise, in soil with TPH-W, the FNB, AZP and AZT inhibitions were 90 %, with the exception of the 14 173 mg.kg-1 d. b. treatment, which stimulated the NFB and AZT in 2 and 0.10 times more than the control, respectively. The DMP was continued at the six months in the experiments, with values of 63 and 89 g in fresh and weathered petroleum, respectively; had no significant differences with the control (p≤0.05). The PDH reached values of 66 to 87 % both TPH-F and TPH-W at six months, respectively. These results demonstrated the ability the L. hexandra rhizosphere to stimulate the high NFB density, vegetal biomass production and phytoremediation of contaminated soils (with fresh and weathered petroleum), in a tropical waterlogging environment.

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