scholarly journals Is Melioidosis a One Health-Neglected Disease in Iran?

2020 ◽  
Vol 8 (3) ◽  
pp. 93-95
Author(s):  
Zahra Baradaran-Seyed
Keyword(s):  
Burkholderia Cepacia ◽  
Burkholderia Pseudomallei ◽  
Soil Structure ◽  
One Health ◽  
Health Surveillance ◽  
Burkholderia Cepacia Complex ◽  
Burkholderia Mallei ◽  
Isolation And Identification ◽  
History Of ◽  
The One

According to the several scientific resources, Iran is considered to be among the melioidosis-endemic regions of the world; this is in stark contrast to the domestic stance in Iran, where the risk of melioidosis is speculated only as an emerging infectious disease in a non-endemic area. Recently, we have had devastating flash floods in the most provinces of Iran; the changing of soil structure may facilitate the cultivation of bacteria in the upper layers of the ground, consequently causes exposure and re-emergence of the disease. Considering previous isolation and identification of Burkholderia mallei, Burkholderia Pseudomallei, and Burkholderia cepacia complex from human, animal, and environment samples in Iran, it is urgently necessary to upgrade and equip infrastructures to identify and distinguish between pathogenic Burkholderia and commensals in the context of the One Health surveillance system. In the present perspective, the history of melioidosis in the country is presented to attract attention to the negligence of disease over the last four decades.

scholarly journals Conservation of resistance nodulation cell division efflux pump mediated antibiotic resistance in Burkholderia cepacia complex and Burkholderia pseudomallei complex species

2021 ◽  
Author(s):  
Nawarat Somprasong ◽  
Jinhee Yi ◽  
Carina M. Hall ◽  
Jessica R. Webb ◽  
Jason W. Sahl ◽  
...  
Keyword(s):  
Cell Division ◽  
Burkholderia Cepacia ◽  
Burkholderia Pseudomallei ◽  
Efflux Pump ◽  
Multidrug Resistant ◽  
Resistance Mechanisms ◽  
Burkholderia Cepacia Complex ◽  
Complex Species ◽  
Functional Studies ◽  
Rnd Efflux Pump

Burkholderia cepacia complex (Bcc) and Burkholderia pseudomallei complex (Bpc) species include pathogens that are typically multidrug resistant. Dominant intrinsic and acquired multidrug resistance mechanisms are efflux mediated by pumps of the resistance nodulation cell division (RND) family. From comparative bioinformatic and, in many instances, functional studies we infer that RND pump-based resistance mechanisms are conserved in Burkholderia . We propose to use these findings as a foundation for adoption of a uniform RND efflux pump nomenclature.

scholarly journals 148 Burkholderia cepacia complex isolation and identification in a Turkish CF Unit

2006 ◽  
Vol 5 ◽  
pp. S34
Author(s):  
P. Yurdaku ◽  
K. Ergunay ◽  
E. Yalcin ◽  
D. Dogru ◽  
B. Sener ◽  
...  
Keyword(s):  
Burkholderia Cepacia ◽  
Burkholderia Cepacia Complex ◽  
Isolation And Identification

scholarly journals Misidentification of Burkholderia pseudomallei and Other Burkholderia Species From Pediatric Infections in Mexico

2019 ◽  
Vol 6 (2) ◽  
Author(s):  
Georgina Meza-Radilla ◽  
Ausel Mendez-Canarios ◽  
Juan Xicohtencatl-Cortes ◽  
Marcos R Escobedo-Guerra ◽  
Alfredo G Torres ◽  
...  
Keyword(s):  
New Species ◽  
Burkholderia Cepacia ◽  
Burkholderia Pseudomallei ◽  
Burkholderia Cepacia Complex ◽  
Burkholderia Species ◽  
Genotypic Analysis ◽  
Pediatric Infections

Abstract Burkholderia pseudomallei and Burkholderia cepacia complex are poorly studied in Mexico. The genotypic analysis of 38 strains isolated from children with pneumonia were identified and showed that both Burkholderia groups were present in patients. From our results, it is plausible to suggest that new species are among the analyzed strains.

scholarly journals Identification of Burkholderia cepacia complex bacteria with a lipopolysaccharide-specific monoclonal antibody

2010 ◽  
Vol 59 (1) ◽  
pp. 41-47 ◽  
Author(s):  
David P. AuCoin ◽  
Reva B. Crump ◽  
Peter Thorkildson ◽  
Dana E. Nuti ◽  
John J. LiPuma ◽  
...  
Keyword(s):  
Burkholderia Cepacia ◽  
Burkholderia Pseudomallei ◽  
Proteinase K ◽  
Burkholderia Cepacia Complex ◽  
Gram Negative Bacteria ◽  
Specific Monoclonal Antibody ◽  
Separate Species ◽  
Gram Negative ◽  
Ladder Pattern ◽  
Human Infections

The genus Burkholderia includes many bacteria that cause serious human infections. As is the case with other Gram-negative bacteria, Burkholderia species produce LPS, which is an abundant component of the bacterial cell surface. Burkholderia cepacia complex (Bcc) bacteria (which include at least 17 separate species) produce LPS structures that are quite different. In an attempt to determine the degree of LPS epitope variation among Bcc species, a mAb was produced, designated 5D8, specific for the LPS of B. cepacia. Western blot analysis determined that mAb 5D8 was able to produce the classic ‘ladder pattern’ when used to probe B. cepacia and Burkholderia anthina lysates, although 5D8 did not produce this pattern with the other seven Bcc species tested. mAb 5D8 reacted with varying intensity to most but not all of the additional B. cepacia and B. anthina strains tested. Therefore, there seems to be significant epitope variation among Bcc LPS both between and within species. Additionally, mAb 5D8 reacted with a proteinase-K-sensitive 22 kDa antigen in all Bcc strains and also in a strain of Burkholderia pseudomallei.

scholarly journals Implementation challenges of an integrated One Health surveillance system in humanitarian settings: A qualitative study in Palestine

2021 ◽  
Vol 9 ◽  
pp. 205031212110430
Author(s):  
Samer Abuzerr ◽  
Kate Zinszer ◽  
Abraham Assan
Keyword(s):  
Qualitative Study ◽  
Surveillance System ◽  
One Health ◽  
Structured Interview ◽  
Health Surveillance ◽  
Effective Control ◽  
Policy Coherence ◽  
Implementation Challenges ◽  
The One ◽  
Training Programmes

Objectives: Several factors have changed interactions between people, animals, plants, and the environment – renewing the relevance of the One Health surveillance system in the fight against zoonotic diseases such as COVID-19. Therefore, this study aimed to explore barriers to implementing an integrated One Health surveillance system in Palestine. Methods: This qualitative study was conducted from April 2020 until August 2020. Data were collected using semi-structured interview guides. Seven key stakeholders were interviewed during data collection. A thematic analysis was performed. Results: Four overarching themes emerged explaining barriers to integrated implementation of the One Health surveillance system. They are lack of policy coherence, limited financial resources, poor governance and leadership, and lack of One Health training programmes. Conclusion: Improved understanding of the transmission and effective control (including One Health approach) of zoonotic disease and better governance and leadership are critical in the diseases that threaten public health, such as the COVID-19.

scholarly journals A multi-country study using MALDI-TOF mass spectrometry for rapid identification of Burkholderia pseudomallei

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Wanitda Watthanaworawit ◽  
Tamalee Roberts ◽  
Jill Hopkins ◽  
Ian Gassiep ◽  
Robert Norton ◽  
...  
Keyword(s):  
Burkholderia Cepacia ◽  
Burkholderia Pseudomallei ◽  
Research Unit ◽  
Rapid Identification ◽  
Geographical Differences ◽  
Isolation And Identification ◽  
Maldi Tof ◽  
The Impact ◽  
Vitek Ms

Abstract Background Burkholderia pseudomallei is the bacterial causative agent of melioidosis, a difficult disease to diagnose clinically with high mortality if not appropriately treated. Definitive diagnosis requires isolation and identification of the organism. With the increased adoption of MALDI-TOF MS for the identification of bacteria, we established a method for rapid identification of B. pseudomallei using the Vitek MS, a system that does not currently have B. pseudomallei in its in-vitro diagnostic database. Results A routine direct spotting method was employed to create spectra and SuperSpectra. An initial B. pseudomallei SuperSpectrum was created at Shoklo Malaria Research Unit (SMRU) from 17 reference isolates (46 spectra). When tested, this initial SMRU SuperSpectrum was able to identify 98.2 % (54/55) of Asian isolates, but just 46.7 % (35/75) of Australian isolates. Using spectra (430) from different reference and clinical isolates, two additional SMRU SuperSpectra were created. Using the combination of all SMRU SuperSpectra with seven existing SuperSpectra from Townsville, Australia 119 (100 %) Asian isolates and 31 (100 %) Australian isolates were correctly identified. In addition, no misidentifications were obtained when using these 11 SuperSpectra when tested with 34 isolates of other bacteria including the closely related species Burkholderia thailandensis and Burkholderia cepacia. Conclusions This study has established a method for identification of B. pseudomallei using Vitek MS, and highlights the impact of geographical differences between strains for identification using this technique.

scholarly journals A Rare Case of Burkholderia cepacia Complex Septic Arthritis

2018 ◽  
Vol 2018 ◽  
pp. 1-3
Author(s):  
John Koo ◽  
Gregory D. Deans
Keyword(s):  
Synovial Fluid ◽  
Septic Arthritis ◽  
Burkholderia Cepacia ◽  
Penetrating Trauma ◽  
Calcium Pyrophosphate ◽  
Opioid Use Disorder ◽  
Burkholderia Cepacia Complex ◽  
Opioid Use ◽  
Irrigation And Debridement ◽  
History Of

Bacteria of the Burkholderia cepacia complex have rarely been reported to cause septic arthritis. Cases have been reported in patients who were immunocompromised, at extremes of age or who had history of steroid injection or penetrating trauma. A 67-year-old man with a history of opioid use disorder, osteoarthritis, and gout but no known immunocompromise was admitted to hospital with pain and swelling of his right knee. Cultures of synovial fluid and urine grew Burkholderia cepacia complex. Microscopy of synovial fluid also identified intracellular calcium pyrophosphate crystals. The patient’s symptoms improved with joint irrigation and debridement and prolonged antimicrobial therapy. This case highlights the importance of diagnostic aspiration of an acutely inflamed joint to obtain a specific etiological diagnosis.

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