scholarly journals Modalidades quimiossensoriais das larvas de Brycon orbignyanus / Chemosensory modalities of Brycon orbignyanus larvae

2021 ◽  
Vol 4 (4) ◽  
pp. 5543-5556
Author(s):  
Cláudia Maria Reis Raposo Maciel ◽  
Alaor Maciel Júnior ◽  
Eduardo Arruda Teixeira Lanna ◽  
Lidiane da Silva Nascimento
Keyword(s):  
Alcian Blue

Com o objetivo de descrever o desenvolvimento das estruturas sensoriais quimiorreceptoras, corpúsculos gustativos e epitélio olfativo, das larvas de piracanjuba, Brycon orbignyanus, entre zero e 172 horas após eclosão, exemplares foram coletados a cada meia hora e fixados em fluído de bouin, por6 a 8 horas, e transferidos para solução de álcool 70%. As lâminas histológicas foram coradas por pelos métodos HE e Alcian Blue. As análises foram realizadas com fotomicroscópio de luz trinocular, acoplado a um microcomputador contendo um software de análise de imagens. Verificou-se que as larvas de piracanjuba, com 28 horas após a eclosão, desenvolveram estruturas quimiossensoriais importantes, como epitélio sensorial na cavidade olfatória e corpúsculos gustativos projetados na cavidade bucofaringiana e nas valvas orais, sugerindo que a quimiorrecepção é requerida na primeira alimentação exógena, que acontece neste momento. às 72 horas após eclosão, verificou-se a cavidade olfatória ampla e funcional e aumento de corpúsculos gustativos na cavidade bucofaringiana, o que revela uma elevada sensibilidade gustatória que, combinada com comportamento alimentar ativo, típico da espécie, sugere habilidade para detectar e selecionar alimentos e ingerí-los ou rejeitá-los, além de reconhecer substâncias tóxicas e venenosas. As larvas de piracanjuba, com 172 horas após eclosão, no final da metamorfose, apresentaram estruturas quimiossensoriais desenvolvidas, lamelas olfatórias formadas e gustação desenvolvida.

Cytochemical Specializations on the Luminal Surface of Blood Vessels in the Developing Rat Central Nervous System

1975 ◽  
Vol 33 ◽  
pp. 462-463
Author(s):  
R. S. Hannah ◽  
T. H. Rosenquist
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Blood Vessels ◽  
Alcian Blue ◽  
Luminal Surface ◽  
High Voltage Electron Microscopy ◽  
Voltage Electron ◽  
Concentration Method ◽  
Endothelial Surface ◽  
Cacodylate Buffer

Developing blood vessels in the rat central nervous system exhibit several unusual luminal features. Hannah (1975) used high voltage electron microscopy to demonstrate numerous ridges of endothelium, some near junctional complexes. The ridges produced troughs (which may appear as depressions) in the endothelial surface. In some areas ridges extended over the troughs, removing them from direct contact with the luminal surface. At no time were the troughs observed to penetrate the basal laminae. Fingerlike projections also extended into the lumina.To determine whether any chemical specializations accompanied the unusual morphological features of the luminal surface, we added 0.1% Alcian blue (Behnke and Zelander, 1970) to the 3% glutaraldehyde perfusate (cacodylate buffer, pH 7.4). After Alcian blue had reacted with the luminal glycocalyces, the dye was dissociated with MgCl2 via critical electrolyte concentration method of Scott and Dorling (1965). When these methods are applied together, it is possible to differentiate mucopolysaccharides (glycosaminoglycans or GAG) with the electron microscope.

scholarly journals Intra-Cystic (In Situ) Mucoepidermoid Carcinoma: A Clinico-Pathological Study of 14 Cases

2020 ◽  
Vol 9 (4) ◽  
pp. 1157
Author(s):  
Saverio Capodiferro ◽  
Giuseppe Ingravallo ◽  
Luisa Limongelli ◽  
Mauro Mastropasqua ◽  
Angela Tempesta ◽  
...  
Keyword(s):  
Alcian Blue ◽  
Tumor Invasion ◽  
Conservative Surgery ◽  
Low Grade ◽  
Mucin Production ◽  
Cystic Component ◽  
Early Growth Phase ◽  
Hematoxylin Eosin

Aims: To report on the clinico-pathological features of a series of 14 intra-oral mucoepidermoid carcinomas showing exclusive intra-cystic growth. Materials and methods: All mucoepidermoid carcinomas diagnosed in the period 1990–2012 were retrieved; the original histological preparations were reviewed to confirm the diagnosis and from selected cases, showing exclusive intra-cystic neoplastic components, additional sections were cut at three subsequent 200 m intervals and stained with Hematoxylin–Eosin, PAS, Mucicarmine and Alcian Blue, to possibly identify tumor invasion of the adjacent tissues, which could have been overlooked in the original histological preparations. Additionally, pertinent findings collected from the clinical charts and follow-up data were analyzed. Results: We identified 14 intraoral mucoepidermoid carcinomas treated by conservative surgery and with a minimum follow up of five years. The neoplasms were located in the hard palate (nine cases), the soft palate (two), the cheek (two) and the retromolar trigone (one). In all instances, histological examination revealed the presence of a single cystic space, containing clusters of columnar, intermediate, epidermoid, clear and mucous-producing cells, the latter exhibiting distinct intra-cytoplasmic mucin production, as confirmed by PAS, Mucicarmine and Alcian Blue stains. The cysts were entirely circumscribed by fibrous connective tissue, and no solid areas or infiltrating tumor cell clusters were detected. Conservative surgical resection was performed in all cases, and no recurrences or nodal metastases were observed during follow up. Conclusions: Mucoepidermoid carcinomas showing prominent (>20%) intra-cystic proliferation currently are considered low-grade tumors. In addition, we also unveil the possibility that mucoepidermoid carcinomas, at least in their early growth phase, may display an exclusive intra-cystic component and might be considered as in situ carcinomas, unable to infiltrate adjacent tissues and metastasize.

High iron diamine?alcian blue mucin profiles in benign, premalignant and malignant colorectal disease

1988 ◽  
Vol 13 (4) ◽  
pp. 399-411 ◽  
Author(s):  
D. C. ALLEN ◽  
N. S. CONNOLLY ◽  
J. D. BIGGART
Keyword(s):  
Alcian Blue ◽  
Colorectal Disease ◽  
High Iron

Human Umbilical Cord Mesenchymal Stem Cells Induce into Chondrocytes in 3D Cultured System

2013 ◽  
Vol 749 ◽  
pp. 198-205
Author(s):  
Li Yu ◽  
Jing Liu ◽  
Chao Xu ◽  
Er Mei Luo ◽  
Ming Qiao Tang
Keyword(s):  
Real Time ◽  
Alcian Blue ◽  
Culture System ◽  
Type Ii Collagen ◽  
Human Umbilical Cord ◽  
Type Ii ◽  
Pellet Culture ◽  
Hla Dr

Objective: To investigate a better method of inducing hUC-MSCs into chondrocytes in different culture system in vitro. Method: hUC-MSCs were isolated and cultured by tissue block culture, and the cells surface antigens were identified by flow cytometry, hUC-MSCs were cultured with chondrogenic media and stained with Alcian Blue. The production of matrix was estimated from the determination of hydroxyproline content and Alcian Blue method. Expressions of glycosaminoglycan (GAG), type II collagen and Sox-9 were assayed by real-time fluorescence quantitative PCR. Results: The cultured hUC-MSCs phenotype was CD105+/CD29+/CD44+/ CD31-/CD34-/ CD40-/CD45-/HLA-DR-. hUC-MSCs weakly expressed chondrocyte marker, which strongly expressed GAG and type II collagen after chondrogenic induction, and the cells were incubated in pellet culture with higher expression. Real-time PCR results demonstrated that chondrogenic induction cells were expressed GAG, type II collagen and Sox-9, and the cells were incubated in pellet culture with higher expression. Conclusion: hUC-MSCs incubated in pellet culture is more conducive to differentiate into chondrocytes than those cultured in monolayer culture system.

Haemopoiesis in Lepidoptera. III. A note on the multiplication of spherule cells and granular haemocytes

1983 ◽  
Vol 61 (1) ◽  
pp. 275-277 ◽  
Author(s):  
J. W. Arnold ◽  
C. F. Hinks
Keyword(s):  
Mitotic Index ◽  
Alcian Blue ◽  
Maximum Production ◽  
Spherule Cell ◽  
Dividing Cells ◽  
Lepidoptera Noctuidae ◽  
Potential Maximum ◽  
Hematoxylin Eosin ◽  
Cell Mitosis

Blood films from early sixth instar larvae of Euxoa declarata (Lepidoptera: Noctuidae) stained in hematoxylin – eosin – alcian blue showed unequivocal examples of mitosis in spherule cells. The improved visibility of mitosis and the estimation of the mitotic index from counts of dividing cells per 1000 cells of each type indicated a far greater potential maximum production of spherule cells and granular haemocytes by mitosis than reported previously. Certain other methods of staining showed similar clear examples of spherule cell mitosis.

The Demonstration of Bone and Cartilage Remodelling Using Alcian Blue and Hematoxylin

1988 ◽  
Vol 63 (1) ◽  
pp. 59-63 ◽  
Author(s):  
D. C. J. Sayers ◽  
G. Volpin ◽  
G. Bentley
Keyword(s):  
Alcian Blue ◽  
And Cartilage

The use of alcian blue in vivo

1967 ◽  
Vol 10 (2) ◽  
pp. 115-125 ◽  
Author(s):  
L. Grant ◽  
F. F. Becker
Keyword(s):  
Alcian Blue

scholarly journals LARYNGOTRACHEITIS VIRUS IN CHICKENS

1967 ◽  
Vol 125 (3) ◽  
pp. 409-428 ◽  
Author(s):  
Betsy G. Bang ◽  
Frederik B. Bang
Keyword(s):  
Alcian Blue ◽  
Plasma Cells ◽  
Giant Cells ◽  
Functional Restoration ◽  
Nasal Fossa ◽  
Blue Staining ◽  
Alcian Blue Staining ◽  
Cell Nuclei ◽  
Histochemical Change ◽  
Laryngotracheitis Virus

Infectious laryngotracheitis can be produced in chickens as an experimental model of severe nonfatal rhinitis and sinusitis. Inoculated intranasally into unanesthetized baby chicks it remains limited to the nasal fossa, produces acute desquamation of all nasal epithelia, results in functional recovery of the respiratory epithelium, but leaves important residual abnormalities. From the earliest recognizable lesions through 4½ months' convalescence, the principal changes are as follows: 1. Initial lesions, or small syncytia of intranuclear "inclusions", first identifiable in the mucociliated cells of the shallowest portion of the epithelium at about 21 hr postinoculum (the inner surface of the maxillary conchal scroll). 2. Acute sloughing, (about 3 to 7 days), marked by: (a) spread of lesions from cell to cell via multinucleated "giant cells" which progressively slough and desquamate respiratory, olfactory, and sinus epithelia, epithelial neural elements and blood vessels; (b) appearance of numbers of eosinophilic leukocytes along the basement membrane at the sites of lesions just previous to sloughing; intensive infiltration of the submucosa with small lymphocytes after sloughing begins; (c) histochemical change in the intracellular mucus of the cells which comprise the syncytia: this mucus stains with Alcian blue alone when stained with AB-PAS; and (d) all cartilages of the maxillary conchae become flaccid, and the cell nuclei and matrix lose both basophilic and Alcian blue staining properties, effects which recede by about the 8th day. 3. Repair (about 8 to 21 days), marked by rapid initial spread of a sheet of epithelial cells over the infiltrated subrmucosa, appearance of numbers of plasma cells circulating in the tissues, formation of encapsulated secondary nodules, and mucosal adhesions. 4. Convalescence (about 1 to 4½ months when experiments terminated), marked by functional restoration of the mucociliary lining of the nasal fossa. However, at 4½ months eight specimens all show complete metaplasia of the olfactory organ (end nerves, supporting cells, and glands of Bowman) to mucociliated epithelium, all show abnormal formation and alignment of mucous acini, and about 50% have severe persistent sinusitis.

Mast Cell Heterogeneity in Human Lung Tissue

1989 ◽  
Vol 77 (3) ◽  
pp. 297-304 ◽  
Author(s):  
F. J. Van Overveld ◽  
L. A. M. J. Houben ◽  
F. E. M. Schmitz du Moulin ◽  
P. L. B. Bruijnzeel ◽  
J. A. M. Raaijmakers ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Alcian Blue ◽  
Lung Tissue ◽  
Human Lung ◽  
Immunoglobulin E ◽  
Prostaglandin D2 ◽  
Leukotriene C4 ◽  
Human Lung Tissue ◽  
No Release

1. In this study mast cells were found to comprise 2.1% of total cells recovered by enzymatic digestion of human lung tissue. 2. This mast cell population consisted of 79% formalin-sensitive, Alcian Blue-positive mast cells and 21% formalin-insensitive, Alcian Blue-positive mast cells. 3. By the use of centrifugal elutriation and subsequent Percoll gradient centrifugation, separate mixed cell populations could be obtained in which the mast cell constituents were either of the formalin-sensitive or -insensitive type. 4. Cell suspensions in which formalin-sensitive cells comprised 97% of mast cells contained approximately 1.34 pg of histamine per mast cell, whereas in preparations in which mast cells were 84% formalin-resistant the histamine content was approximately 4.17 pg of histamine per mast cell. 5. The histamine release upon anti-immunoglobulin E challenge of formalin-sensitive mast cells was greater than the release by formalin-insensitive mast cells. 6. After challenge with opsonized zymosan, only formalin-sensitive mast cells were able to release histamine. 7. Leukotriene C4 release was observed when formalin-sensitive mast cells were challenged with antiimmunoglobulin E. Formalin-insensitive mast cells showed no release of leukotriene C4. 8. Prostaglandin D2 release was observed when formalin-insensitive mast cells were challenged with antiimmunoglobulin E. Formalin-sensitive mast cells showed no release of prostaglandin D2.

Sign in / Sign up

Export Citation Format

Share Document