Гистохимический анализ 3β-гидроксистероиддегидрогеназы в гипоталамусе крысы

2020 ◽  
pp. 38-41
Keyword(s):  
Positive Reaction ◽  
Paraventricular Nucleus ◽  
Gray Matter ◽  
Hydroxysteroid Dehydrogenase ◽  
Small Cell ◽  
Histochemical Method ◽  
Peripheral Part ◽  
Arcuate Nuclei ◽  
Different Parts

3β-hydroxysteroid dehydrogenase (GSDG) in different parts of the hypothalamus was studied by histochemical method. It was shown that GSDG-positive reaction was present in neurons of the periventricular gray matter, ventromedial, ventrolateral, and arcuate nuclei of the hypothalamus. In the paraventricular nucleus, GSDG-positive neurons were «small-cell» neurons located primarily in the peripheral part of this nucleus.

Increased expression of 17-beta-hydroxysteroid dehydrogenase type 1 in non-small cell lung cancer

Lung Cancer ◽  
2015 ◽  
Vol 87 (2) ◽  
pp. 107-116 ◽  
Author(s):  
Hanna Drzewiecka ◽  
Bartłomiej Gałęcki ◽  
Donata Jarmołowska-Jurczyszyn ◽  
Andrzej Kluk ◽  
Wojciech Dyszkiewicz ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Hydroxysteroid Dehydrogenase ◽  
Small Cell ◽  
Small Cell Lung

scholarly journals 11β-Hydroxysteroid Dehydrogenase Type 2 Activity in Hypothalamic Paraventricular Nucleus Modulates Sympathetic Excitation

Hypertension ◽  
2006 ◽  
Vol 48 (1) ◽  
pp. 127-133 ◽  
Author(s):  
Zhi-Hua Zhang ◽  
Yu-Ming Kang ◽  
Yang Yu ◽  
Shun-Guang Wei ◽  
Thomas J. Schmidt ◽  
...  
Keyword(s):  
Paraventricular Nucleus ◽  
Hydroxysteroid Dehydrogenase ◽  
Hypothalamic Paraventricular Nucleus

scholarly journals Fasting Activates the Nonhuman Primate Hypocretin (Orexin) System and Its Postsynaptic Targets

Endocrinology ◽  
2003 ◽  
Vol 144 (9) ◽  
pp. 3774-3778 ◽  
Author(s):  
Sabrina Diano ◽  
Balazs Horvath ◽  
Henryk F. Urbanski ◽  
Peter Sotonyi ◽  
Tamas L. Horvath
Keyword(s):  
Brain Activity ◽  
Brain Regions ◽  
Thalamic Nuclei ◽  
Dorsomedial Nucleus ◽  
Leptin Receptors ◽  
Axon Terminals ◽  
Hypothalamic Regulation ◽  
Hypothalamic Cells ◽  
Arcuate Nuclei ◽  
Different Parts

Abstract In rodents, hypocretin (HCRT, also called orexin) influences a variety of endocrine, autonomic, and metabolic functions. The present study was undertaken to determine whether the HCRT-producing circuit is involved in the hypothalamic regulation of homeostasis in primates as well. We studied female monkeys (Cercopithecus aethiops) that were either fed or fasted for 24 h. Immunocytochemistry revealed HCRT-producing perikarya exclusively in the lateral hypothalamus-perifornical region and dorsomedial hypothalamus of the monkey brain. HCRT axons and axon terminals were present in different parts of the hypothalamus and adjacent forebrain and thalamic nuclei. The 24-h fast resulted in an approximately 50% decline in circulating leptin levels and significantly elevated c-fos expression in the perifornical region; in the dorsomedial, ventromedial, and arcuate nuclei; and in the medial preoptic area. In the dorsomedial nucleus and perifornical region of fasted monkeys, three times more HCRT-neurons expressed nuclear c-fos than those of the normally fed controls. Neurons in different parts of the hypothalamus and basal forebrain that expressed c-fos, but did not contain HCRT, were targets of HCRT-immunopositive boutons establishing asymmetric synapses. In the arcuate nucleus, subsets of these HCRT-targeted c-fos-expressing cells contained neuropeptide Y. The present study provides the first experimental evidence to implicate HCRT in the hypothalamic regulation of homeostasis in primates. The fact that these lateral hypothalamic cells have leptin receptors and can be activated by a metabolic challenge and that they innervate diverse brain regions indicates that the HCRT system may be a key integrator of environmental cues in their regulation of diverse brain activity.

scholarly journals AGE FEATURES OF THE MORPHOLOGY OF TONGUE GLANDS

2017 ◽  
Vol 25 (4) ◽  
pp. 43-45
Author(s):  
A. V. Olsuf’Eva ◽  
A. D. Vovkogon ◽  
M. O. Timofeeva ◽  
L. V. Veselova ◽  
S. S. Olsuf’Ev
Keyword(s):  
Oral Cavity ◽  
Morphological Changes ◽  
Tap Water ◽  
Internal Organs ◽  
Peripheral Part ◽  
Mucous Membranes ◽  
Parametric Statistics ◽  
Blue Solution ◽  
Age Features ◽  
Different Parts

Tongue provides a person with many functions, participates in speech, taste receptions (the peripheral part of the taste analyzer), the initial stages of digestion and moisturizes the mucous membrane of the oral cavity. The lingual glands, along with others, produce saliva, but in a smalt amount, in comparison with the large salivary glands. The purpose of the study is to study the number of lingual glands and their morphological features in people of different ages and sex under normal conditions. Macroscopic, histological methods and parametric statistics methods have been used to study the glands of the tongue, obtained from the corpses of 149 men and 150 women who died from the period of the newborn to 96 years, for reasons of asphyxia incompatible with the life of injuries. On total preparations of the tongue, the glands were electrically stained in a 0.5% solution of acetic acid with 0,05% methylene blue solution in tap water. We studied the total number, length and width of the initial sections of the glands of different parts of the tongue - at its anterior, middle and posterior third, and also in the organ as a whole. For histological examination, the gland was further studied on preparations obtained after evisceration (139 cases). As a result of the study, it was revealed that the size and quantity of lingual glands in postnatal ontogenesis vary in the direction from the tip to the root of the tongue (increase from front to back). In this case, the glands of the root of the tongue produced a saliva of a mucous nature. Given that ensuring the oral phase of swallowing requires the presence of a mucous secretion in the oral cavity, the morphological changes of lingual glands in the thickness of the tongue revealed by us are absolutely physiologically necessary. Changing the size and number of glands throughout the mucous membranes of internal organs is recognized as one of the patterns of their structural organization.

scholarly journals Age-Related Changes in Zinc, Copper and Selenium Levels in the Human Prostate

Nutrients ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 1403
Author(s):  
Adam Daragó ◽  
Michał Klimczak ◽  
Joanna Stragierowicz ◽  
Mateusz Jobczyk ◽  
Anna Kilanowicz
Keyword(s):  
Prostate Gland ◽  
Essential Elements ◽  
Prostatic Hyperplasia ◽  
Peripheral Part ◽  
Age Related ◽  
Aas Method ◽  
Spectrofluorometric Method ◽  
Low Levels ◽  
Different Parts ◽  
Age Related Changes

Pathophysiological changes in the prostate gland—benign prostatic hyperplasia (BPH) and prostatic adenocarcinoma (PCa)—are closely related to the age of men. In the prostate gland, zinc is of particular importance for its proper functioning, especially with regard to the effects of hormonal disorders. The aim of this study was to evaluate zinc, copper and selenium concentrations in different parts of the prostate gland in relation to age and the nature of pathological changes. Zinc and copper were determined by the AAS method and selenium by the spectrofluorometric method. The concentration of zinc in the central part of the prostate increases with age, and in patients over 36 years it is twice as high as in the peripheral part, where no increase in the level of this element was observed with the age of patients. The above data confirm a possible influence of zinc on the formation of PCa (located mostly in the peripheral part of the prostate, with low levels of zinc) and BPH in the central part where the levels of this element are the highest. The results apparently confirm the disturbed homeostasis of zinc and other essential elements in the etiology of BPH and PCa.

scholarly journals The Distribution of Cells Containing Estrogen Receptor-α (ERα) and ERβ Messenger Ribonucleic Acid in the Preoptic Area and Hypothalamus of the Sheep: Comparison of Males and Females

Endocrinology ◽  
2000 ◽  
Vol 141 (8) ◽  
pp. 2951-2962 ◽  
Author(s):  
Christopher J. Scott ◽  
Alan J. Tilbrook ◽  
Donna M. Simmons ◽  
John A. Rawson ◽  
Simon Chu ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Sex Differences ◽  
Paraventricular Nucleus ◽  
Arcuate Nucleus ◽  
Preoptic Area ◽  
Estrogen Receptor Α ◽  
Lateral Septum ◽  
Ventromedial Nucleus ◽  
Arcuate Nuclei ◽  
Silver Grains

We have used in situ hybridization to compare the distributions of estrogen receptor α (ERα) and ERβ messenger RNA (mRNA)-containing cells in the preoptic area and hypothalamus of ewes and rams. Perfusion-fixed brain tissue was collected from luteal phase ewes and intact rams (n = 4) during the breeding season. Matched pairs of sections were hybridized with sheep-specific, 35S-labeled riboprobes, and semiquantitative image analysis was performed on emulsion-dipped slides. A number of sex differences were observed, with females having a greater density of labeled cells than males (P < 0.001) and a greater number of silver grains per cell (P < 0.01) in the ventromedial nucleus for both ER subtypes. In addition, in the retrochiasmatic area, males had a greater (P < 0.05) cell density for ERα mRNA-containing cells than females, whereas in the paraventricular nucleus, females had a greater density (P < 0.05) of ERα mRNA-containing cells than males. There was a trend (P = 0.068) in the arcuate nucleus for males to have a greater number of silver grains per cell labeled for ERα mRNA. In both sexes, there was considerable overlap in the distributions of ERα and ERβ mRNA-containing cells, but the density of labeled cells within each nucleus differed in a number of instances. Nuclei that contained a higher (P < 0.001) density of ERα than ERβ mRNA-containing cells included the preoptic area, bed nucleus of the stria terminalis, and ventromedial nucleus, whereas the subfornical organ (P < 0.001), paraventricular nucleus (males only, P < 0.05), and retrochiasmatic nucleus (females only, P < 0.05) had a greater density of ERα than ERβ mRNA-containing cells. The anterior hypothalamic area and supraoptic nucleus had similar densities of cells containing both ER subtypes. The lateral septum and arcuate nucleus contained only ERα, whereas only ERβ mRNA-containing cells were seen in the zona incerta. The sex differences in the populations of ER mRNA-containing cells in the ventromedial and arcuate nuclei may explain in part the sex differences in the neuroendocrine and behavioral responses to localized estrogen treatment in these nuclei. Within sexes, the differences between the distributions of ERα and ERβ mRNA-containing cells may reflect differential regulation of the actions of estrogen in the sheep hypothalamus. Low levels of ERβ mRNA in the preoptic area and ventromedial and arcuate nuclei, regions known to be important for the regulation of reproduction, suggest that ERβ may not be involved in these functions.

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