This research was aimed to examine the moderating role of gender on the impact of sexual jealousy on partner infidelity among university students. For the purpose, a sample of 352 students were recruited through simple random sampling technique from four cities (Bahawalpur, Multan, Lahore, Rawalpindi) of Punjab, Pakistan. Respondents were given two questionnaires; Interpersonal Jealousy Scale and Infidelity Questionnaire. After data collection, results were analysed through SmartPLS (3.0) for moderation model. The results showed that sexual jealousy had significant positive impact showing 59% variance on partner infidelity, whereas, gender was found to be a significant moderator between sexual jealousy and partner infidelity (R2= 0.594, t= 2.403, p<.01). The results of this research are discussed in detail with limitations and future suggestions for the future researches related to impact of sexual jealousy on partner infidelity.