scholarly journals Pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial di Instalasi Gawat Darurayt RSAD Robert Wolter Mongisidi Manado

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Sitti N. Gobel ◽  
Fredine E.S. Rares ◽  
Heriyannis Homenta
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Emergency Room ◽  
Medical Equipment ◽  
Ambient Air ◽  
Aerobic Bacteria ◽  
Bacillus Sp ◽  
Enterobacter Agglomerans ◽  
Hospital Acquired Infection ◽  
Hospital Acquired

Abstract: Nosocomial infection or Hospital Acquired infection (HAI) is caused by bacteria, parasites or viruses in hospitals and occurs after ≥48 hours of hospital admission. Emergency room is te starting place procedures and or medical measures so that the room IGD became one of the places that produces a lot of waste of medical procedures performed and as one of the places most likely to transmit the infection. This study was aimed to determine the sources and patterns of aerobic bacteria that could potentially cause nosocomial infections: in the walls, floors, medical equipment, and ambient air of Emergency Room Robert Wolter Mongisidi Hospital, Manado. This was a descriptive prospective study with a cross sectional design. Samples were bacteria in the walls, floors, medical equipment, and the ambient air of the room IGD in the emergency room, Robert Wolter Mongisidi Hospital, Manado. The results showed that of the 29 samples taken, one sample did not contain any bacteria. There were eight types of bacteria found: Bacillus sp (33%), Staphylococcus sp (27%) Enterobacter agglomerans (13%), Escherichia coli (10%), Streptococcus sp (10%), Serratia marcescens (3%), Neisseria sp (2%) and Klebsiella pneumoniae (2%). Conclusion: The most common bacteria was Bacillus sp.Keywords: IGD, nosocomial infections, patterns of aerobic bacteria Abstrak: Infeksi nosokomial atau Hospital Acquired infection (HAI) merupakan infeksi yang disebabkan oleh bakteri, parasit atau virus di rumah sakit, terjadi ≥ 48 jam sejak masuk rumah sakit. Ruangan IGD merupakan tempat awal pasien mendapatkan prosedur dan atau tindakan medis sehingga ruangan IGD menjadi salah satu tempat yang banyak menghasilkan limbah hasil prosedur dan tindakan medis yang dilakukan serta salah satu tempat yang paling mungkin menularkan infeksi. Penelitian ini bertujuan untuk mengetahui sumber dan pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial: di dinding, lantai, peralatan medis, dan udara di ruang IGD pada ruang gawat darurat medik RSAD Robert Wolter Mongisidi Manado. Jenis penelitian ialah deskriptif prospektif dengan desain otong lintang. Sampel penelitian ialah bakteri pada dinding, lantai, peralatan medis, dan udara di ruang gawat darura tmedik RSAD Robert Wolter Mongisidi Manado. Hasil penelitian memperlihatkan dari 29 sampel yang diambil 1 sampel tidak terdapat bakteri. Terdapat 8 jenis bakteri yang ditemukan yaitu: Bacillus sp (33%), Staphylococcus sp (27%), Enterobacter agglomerans (13%), Escherichia coli (10%), Streptococcus sp (10%), Serratia marcescens (3%), Neisseria sp (2%) dan Klebsiella Pneumoniae (2%). Simpulan: Bakteri yang paling banyak ditemukan ialah Bacillus sp. Kata kunci: IGD, infeksi nosokomial, pola bakteri aerob

scholarly journals ISOLASI DAN IDENTIFIKASI BAKTERI AEROB YANG BERPOTENSI MENJADI SUMBER PENULARAN INFEKSI NOSOKOMIAL DI IRINA A RSUP PROF. DR. R. D. KANDOU MANADO

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Yulya Margaretha Longadi ◽  
Olivia Waworuntu ◽  
Standy Soeliongan
Keyword(s):  
Bacillus Subtilis ◽  
Nosocomial Infection ◽  
Enterobacter Aerogenes ◽  
Aerobic Bacteria ◽  
Enterobacter Agglomerans ◽  
Cross Sectional ◽  
Hospital Acquired Infection ◽  
Serratia Liquefaciens ◽  
Two Samples ◽  
Hospital Acquired

Abstract: Nosocomial infection or Hospital Acquired Infection (HAI) is an infection caused by bacteria, parasite, or virus in the hospital, infection occur at least 72 hours since hospitalized. This infection occurs due to lack of hygiene of the environment causing microorganism infection from environment to human, infection can also occur due to transmission of microorganism from one patient to other patients. Inpatients potentially have very high risk of nosocomial infection occur due to continuous requiring treatment for more than 24 hours. Purpose: To determine the existence of aerobic bacteria that could potentially be the source of transmission of nosocomial infection in Irina A RSUP Prof. Dr. R. D. Kandou Manado. Method: This research was descriptive with cross sectional approach. Fourteen samples were taken from the surface of medical equipment, bed, floor, and wall of the treatment room and eight samples were taken from the air. Identification of bacteria was performed by culture on agar medium, staining gram, and biochemical test. Result: Bacillus subtilis found in nine samples (41%), Serratia liquefaciens found in five samples (22,7%), Lactobacillus found in two samples (9,1%), Staphylococcus found in two samples (9,1%), Coccus Gram negative found in two samples (9,1%), Enterobacter aerogenes found in one sample (4,5%), and Enterobacter agglomerans found in one sample (4,5%). Conclusion: Bacillus subtilis is the most bacteria which had been found in this research.Keywords: Aerobic bacteria, Nosocomial infection, Irina A.Abstrak: Infeksi nosokomial atau Hospital Acquired Infection (HAI) merupakan infeksi yang disebabkan oleh bakteri, parasit, atau virus di rumah sakit, infeksi ini timbul sekurang-kurangnya 72 jam sejak masuk rumah sakit. Infeksi ini terjadi akibat kurang bersihnya lingkungan perawatan menyebabkan terjadinya infeksi mikroorganisme dari lingkungan ke manusia, infeksi juga dapat terjadi akibat berpindahnya mikroorganisme dari pasien yang satu ke pasien yang lain. Pasien ruang rawat inap mempunyai risiko yang sangat tinggi mendapatkan infeksi nosokomial, hal ini dikarenakan pasien pada ruang rawat inap memerlukan asuhan, perawatan dan pengobatan secara berkesinambungan lebih dari 24 jam. Tujuan: Untuk mengetahui adanya bakteri aerob yang berpotensi menjadi sumber penularan infeksi nosokomial di IRINA A RSUP Prof. Dr. R. D. kandou Manado. Metode: Metode penelitian yang digunakan adalah deskriptif dengan pendekatan cross sectional study. Sampel diambil dari 14 usapan permukaan peralatan medis, tempat tidur, lantai, dan dinding ruang perawatan dan delapan sampel dari udara ruang. Identifikasi bakteri dilakukan kultur pada media agar, pewarnaan gram, dan uji biokimia. Hasil: Bacillus subtilis ditemukan sebanyak sembilan sampel (41%), Serratia liquefaciens sebanyak lima sampel (22,7%), Lactobacillus sebanyak dua sampel (9,1%), Staphylococcus sebanyak dua sampel (9,1%), Kokus Gram negatif sebanyak dua sampel (9,1%), Enterobacter aerogenes sebanyak satu sampel (4,5%), dan Enterobacter agglomerans sebanyak satu sampel (4,5%). Kesimpulan: Bacillus subtilis merupakan bakteri yang paling banyak ditemukan.Kata Kunci: Bakteri aerob, Infeksi nosokomial, Irina A.

scholarly journals Isolasi dan identifikasi bakteri aerob yang dapat meyebabkan infeksi nosokomial di Ruang Bedah Mata RSUP Prof. Dr. R. D. Kondou Manado

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Nur Hasana M. N. A. ◽  
Fredine E. S. Rares ◽  
John Porotu’o
Keyword(s):  
Nosocomial Infections ◽  
Medical Instrument ◽  
Bacterial Pathogen ◽  
Enterobacter Aerogenes ◽  
Ambient Air ◽  
Bacillus Sp ◽  
Enterobacter Agglomerans ◽  
Isolation And Identification ◽  
Eye Surgery ◽  
Hospital Acquired

Abstract: Nosocomial infection, also called as the hospital-acquired infection, is infection that occurs in the hospital by bacterial pathogen originated from the hospital, and the occurence of infection 48 hours after hospitalization or patients who were treated longer. This study was aimed to determine types of bacteria that coud cause nosocomial infections in eye surgery room of Prof. Dr. R. D. Kandou General Hospital Manado. This was a descriptive prospective study. Samples were obtained from the floor, wall, operation table, medical instrument, and ambient air in the surgety room, then the samples were performed isolation and identification of the bacteria in the samples. The results showed that there were 6 types of bacteria, as follows: Bacillus sp, Enterobacter agglomerans, Staphylococccus sp, Enterobacter aerogenes, Lactobacillus sp, and Neisseria sp. The most common bacteria found in the samples was Enterobacter agglomerans as many as 9 samples (42. 9%).Keywords: aerobic bacteria, nosocomial infections, eye surgery roomAbstrak: Infeksi Nosokomial atau disebut juga infeksi rumah sakit adalah infeksi yang terjadi di rumah sakit oleh kuman yang berasal dari rumah sakit, timbulnya infeksi sesudah 48 jam perawatan pada pasien rawat inap atau pada pasien yang di rawat lebih lama. Penelitian ini bertujuan untuk mengetahui jenis bakteri yang dapat menyebabkan infeksi nosokomial di ruang bedah mata RSUP Prof. Dr. R. D. Kandou Manado. Jenis penelitian ialah deskriptif prospektif. Sampel diperoleh lantai, dinding, meja operasi, alat medis, dan udara di ruang bedah mata RSUP Prof. Dr. R. D. Kandou Manado kemudian dilakukan isolasi dan identifikasi terhadap bakteri yang terdapat pada sampel. Hasil penelitian dari keseluruhan sampel didapatkan 6 bakteri secara umum yaitu: Bacillus sp, Enterobacter agglomerans, Staphylococccus sp, Enterobacter aerogenes, Lactobacillus sp, dan Neisseria sp. Bakteri terbanyak dari keseluruhan sampel yaitu Enterobacter agglomerans sebanyak 9 sampel (42,9%).Kata kunci: bakteri aerob, infeksi nosokomial, ruang bedah mata

scholarly journals Pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial di ruangan Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO) RSUP Prof. Dr. R. D. Kandou Manado

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Rizka Matoka ◽  
Olivia Waworuntu ◽  
Fredine Rares
Keyword(s):  
Health Care ◽  
Klebsiella Pneumoniae ◽  
Nosocomial Infection ◽  
Emergency Room ◽  
Enterobacter Aerogenes ◽  
Aerobic Bacteria ◽  
Bacillus Sp ◽  
Cross Sectional ◽  
Serratia Liquefaciens ◽  
Health Care Associated Infection

Abstract: Nosocomial infection or Health-care Associated Infection (HAIs) according to the WHO in 2011 are infection to patient acquired during a hospital, after ≥48 hours and ≤30 days after out of the hospital. Factor of infection can be differentiated between patients, nurses, doctors, hospital employed, hospital environment and patient visitor or family. The purpose to know the pattern of aerobic bacteria that could potentially to involve nosocomial infection in the Emergency room of Obstetric and Gynecology (IRDO) Prof. Dr. R. D. Kandou Manado. Type a descriptive observational research with cross sectional study approach. The sampling on the walls, floors, medical equipment, room furniture and the air in the IRDO Prof. Dr. R. D. Kandou Hospital Manado. The results of the 27 samples found bacteria Bacillus sp.(46,55%), Staphylococcus sp. (18,97%), Streptococcus sp. (10,34%), Klebsiella pneumoniae (8,62%), Enterobacter aerogenes, Serratia liquefaciens, Enterobacter cloacea, proteus sp. (3,45%), Neisseria sp. (1,72%).Conclusion: The most aerobic bacteria in the Emergency room of Obstetric and Gynecology (IRDO) Prof. Dr. R. D. Kandou Manado is Bacillus sp.(46,55%).Keywords: Emergency Obstetric and Gynecology, nosocomial infection, aerobic bacteria. Abstrak: Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO) adalah suatu instalasi penanggulangan awal penderita rawat darurat untuk mencegah kematian, dalam menangani kesehatan reproduksi wanita saat tidak hamil ataupun pada saat hamil, persalinan, dan nifas. Infeksi nosokomial atau Health-care Associated Infection (HAIs) menurut WHO 2011 merupakan infeksi yang didapat pasien selama di rumah sakit, setelah ≥ 48 jam dan ≤ 30 hari setelah keluar dari rumah sakit. Faktor terjadinya infeksi bisa dibebakan antar pasien, perawat, dokter, karyawan rumah sakit, lingkungan rumah sakit, dan pengunjung atau keluarga pasien. Tujuan untuk mengetahui pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial pada ruangan Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO) RSUP Prof. Dr. R. D. Kandou Manado. Jenis penelitian deskriptif observational dengan pendekatan studi cross sectional. Pengambilan sampel pada dinding, lantai, peralatan medis, perabotan ruangan, udara di ruangan Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO) RSUP Prof. Dr. R. D. Kandou Manado. Hasil penelitian dari 27 sampel ditemukan bakteri Bacillus sp.(46,55%), Staphylococcus sp. (18,97%), Streptococcus sp. (10,34%), Klebsiella pneumoniae (8,62%), Enterobacter aerogenes, Serratia liquefaciens, Enterobacter cloacea, proteus sp. (3,45%), Neisseria sp. (1,72%). Simpulan: Bakteri aerob di ruangan Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO) RSUP Prof. Dr. R. D. Kandou Manado yang terbanyak ialah Bacillus sp.(46,55%).Kata kunci: Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO), Infeksi nosokomial, bakteri aerob

scholarly journals Mikroorganisme sebagai agen bioremediasi limbah merkuri (Hg) penambangan emas

2019 ◽  
Vol 2 ◽  
Author(s):  
Anggi Reza Pramesti ◽  
Sella Mustika ◽  
Nurul Habibah ◽  
Sofia Puspitarini ◽  
Meichica Serlie ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Proteus Mirabilis ◽  
Enterobacter Aerogenes ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Enterobacter Agglomerans ◽  
Morganella Morganii ◽  
Brevundimonas Vesicularis

Banyaknya kegiatan Penambangan Emas Tanpa Ijin (PETI) di Indonesia menjadi sumber utama terjadinya pencemaran limbah merkuri. Merkuri merupakan salah satu jenis polutan logam berat yang bersifat toksik. Merkuri menimbulkan masalah serius pada kesehatan manusia karena dapat terakumulasi pada tubuh dan bersifat neurotoksin. Beberapa bakteri memiliki kemampuan resisten terhadap merkuri. Sejumlah bakteri yang resisten terhadap limbah merkuri telah diisolasi dari berbagai lokasi pertambangan emas di Indonesia. Beberapa bakteri lokal tersebut dirangkum dalam penelitian ini. Bakteri tersebut diantaranya yaitu : Bacillus sp., Escherichia coli, Enterobacter cloacea, Enterobacter aerogenes, Klebsiella pneumoniae, Alcaligenes sp., Pseudomonas sp., Enterobacter agglomerans, Proteus vugaris, Proteus mirabilis, Enterobacter hafniae, Kliebsella sp., Lactobacillus sp., Morganella morganii, Brevundimonas vesicularis, Nitrococcus mobilis, Fusobacterium aquatile, dan Fusobacterium necrogenes. Bakteri yang resisten terhadap merkuri tersebut dapat dimanfaatkan sebagai agen untuk remediasi wilayah yang tercemar limbah merkuri

scholarly journals POLA BAKTERI AEROB YANG BERPOTENSI MENYEBABKAN INFEKSI NOSOKOMIAL DI RUANG ICU BLU RSUP PROF. DR. R. D. KANDOU MANADO

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Prilly V. Londok ◽  
Heriyannis Homenta ◽  
Velma Buntuan
Keyword(s):  
Bacillus Subtilis ◽  
Nosocomial Infections ◽  
Medical Equipment ◽  
Pathogenic Bacteria ◽  
Enterobacter Cloacae ◽  
Ambient Air ◽  
Aerobic Bacteria ◽  
Klebsiella Pneumonia ◽  
Enterobacter Agglomerans ◽  
Serratia Rubidaea

Abstract: Infections that can be found during the treatment in the ICU are due to contamination of pathogenic bacteria as the source of nosocomial infections. This nosocomial infections appear and show symptoms in patients treated or after being treated in the hospital. This study aimed to determine the sources and patterns of aerobic bacteria that could potentially cause nosocomial infections: in the walls, floors, medical equipment, and in the ambient air of ICU Room, Prof. Dr. R. D. Kandou Hospital, Manado. This was a descriptive study designed with a prospective approach. Samples were bacteria in the walls, floors, medical equipment, and the ambient air of the ICU Room. The results showed that of the 27 samples taken, there were 24 bacteria found: Enterobacter agglomeranse (25%), Bacillus subtilis (25%), Enterobacter cloacae (16.7%), Staphylococcus sp. (8.4%), Gram-negative cocci (16.4%), Serratia rubidaea (4.1%), and Klebsiella pneumonia (4.1%).Keywords: ICU, nosocomial infections, patterns of aerobic bacteriaAbstrak: Infeksi yang dapat ditemukan pada perawatan di ICU karena terkontaminasi dengan sumber bakteri patogen yaitu infeksi nosokomial. Infeksi nosokomial adalah infeksi yang muncul dan menunjukkan gejala selama pasien dirawat atau setelah dirawat di rumah sakit. Penelitian ini bertujuan untuk mengetahui sumber dan pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial di dinding, lantai, peralatan medis, dan udara di ruang ICU BLU RSUP Prof. dr. R. D. Kandou Manado. Desain penelitian ini bersifat deskriptif dengan pendekatan prospektif. Sampel penelitian ialah bakteri pada dinding, lantai, peralatan medis, dan udara di ruang ICU BLU RSUP Prof. Dr. R. D. Kandou Manado. Hasil penelitian memperlihatkan dari 27 sampel yang diambil, terdapat 24 bakteri yaitu Enterobacter agglomerans (25%), Bacillus subtilis (25%), Enterobacter cloacae (16,7%), Staphylococcus sp. (8,4%), Kokus Gram negatif (16,4%), Serratia rubidaea (4,1%), dan Klebsiella pneumonia (4,1%).Kata kunci: ICU, infeksi nosokomial, pola bakteri aerob

Effect of Trans-Cinnamaldehyde on preformed biofilm and biofilm formation of Escherichia coli isolated from urine specimens

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Eman Adel El-Haddad ◽  
Soha Abdel Rahman El-Hady ◽  
Amira Esmail Abdel Hamid ◽  
Hisham Abdel Majeed Fahim
Keyword(s):  
Escherichia Coli ◽  
Biofilm Formation ◽  
Defense Mechanisms ◽  
Colorimetric Assay ◽  
Biofilm Inhibition ◽  
Hospital Acquired Infection ◽  
E Coli ◽  
Before And After ◽  
Tract Infections ◽  
Hospital Acquired

Abstract Introduction Bacteria in most environments exist as communities of sessile cells in a selfproduced polymeric matrix known as biofilms. Biofilms are responsible for more than 80% of infections, including urinary tract infections (UTI). UTI is the most common hospital acquired infection, caused mainly by Escherichia coli (E.coli). E. coli can readily form biofilm in such infections, specially in the presence of indwelling urinary catheter. It’s difficult to eradicate bacteria in biofilms, since they are shielded from the host defense mechanisms as phagocytes and antibodies, as well as antibiotics. Searching for alternative or adjuvant substances for prevention and eradication of biofilm associated infections are therefore urgently needed. Aim of the work Studying the efficacy of the trans-cinnamaldehyde (TC) for preventing E. coli biofilm formation. Materials and methods Thirty isolates of E.coli were obtained from urine samples. To test the effect of TC on E.coli biofilm formation and preformed biofilms, microtitre plates (MTP) were inoculated with the isolated E.coli and were treated with different concentrations of TC and incubated at 37° C. A colorimetric assay was used to assess biofilm inhibition and inactivation and optical densities (OD) were compared before and after adding different TC concentrations. Results The mean OD of the isolated E.coli biofilms was 1.3 and significantly decreased when mixed with TC different concentrations. TC had high activity in inhibition of preformed E.coli biofilms, where no biofilm was detected on MTP treated with 1.25% and 1.5% TC. Conclusion TC inhibited the biofilm forming ability of E.coli isolates could fully inactivate formed biofilms, suggesting its possibility to be used as an anti-biofilm agent or adjuvant in preventing and treating UTI caused by biofilm producing E.coli.

scholarly journals Transcriptional response of Mar, Sox and Rob regulon against concentration gradient carbapenem stress within Escherichia coli isolated from hospital acquired infection

2020 ◽  
Author(s):  
Shiela Chetri ◽  
Bhaskar Jyoti Das ◽  
Deepshikha Bhowmik ◽  
Debadatta Dhar Chanda ◽  
Atanu Chakravarty ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Concentration Gradient ◽  
Efflux Pump ◽  
Transcriptional Response ◽  
Multiple Antibiotic Resistance ◽  
Pump System ◽  
Hospital Acquired Infection ◽  
Outer Membrane Porin ◽  
Hospital Acquired

Abstract Objective The present study was carried out to investigate the transcriptional response of marA (Multiple antibiotic resistance A gene), soxS (Superoxide S gene) and rob (Right-origin-binding gene) under carbapenem stress. Results 12 isolates over-expressing AcrAB-TolC efflux pump system and showing reduced expression of OmpF (Outer membrane porin) gene were selected for further study. Among them, overexpression of marA and rob was observed in 7 isolates. Increasing pattern of expression of marA and rob against meropenem was observed. The clones of marA and rob showed reduced susceptibility towards carbapenems.

scholarly journals Isolasi dan identifikasi bakteri aerob yang berpotensi menjadi sumber penularan infeksi nosokomial di ruang ICU Rsad Robert Wolter Mongisidi Teling Manado

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Hilary G. Sarlin ◽  
Heriyannis Homenta ◽  
John Porotu'o
Keyword(s):  
Klebsiella Pneumoniae ◽  
Serratia Marcescens ◽  
Nosocomial Infections ◽  
Enterobacter Cloacae ◽  
Cross Sectional Study ◽  
Aerobic Bacteria ◽  
Bacillus Sp ◽  
Cross Sectional ◽  
Serratia Liquefaciens ◽  
Serratia Rubidaea

Abstract: Nosocomial infections are infections that arise during the patient was hospitalized and starting to show symptoms for a person being treated or after completion of treatment. Patients in the ICU may have a tendency to nosocomial infections. The purpose of the study is to identify and knowing the most common aerobic bacteria that cause nosocomial infections in the ICU RSAD Robert Wolter Monginsidi Teling Manado.This research is a descriptive cross sectional study. The study samplesare bacteria on the floor, walls, air, bed, and ECG in the ICU RSAD Teling Robert Wolter Monginsidi Manado. The results showed 26 samples out of 26 samples contained bacteria, Bacillus sp. eight samples (30.7%), Enterobacter aerogens six samples (23%), Staphylococcus sp. six samples (23%) and Enterobacter cloacae, Serratia liquefaciens, Serratia rubidaea, Serratia marcescens, Klebsiella pneumoniae, Proteus sp. each found as many as one sample (3.8%). Conclusion: The most common bacteria is Bacillus sp.Keywords: nosocomial infection, ICU, aerobic bacteria Abstrak: Infeksi nosokomial adalah Infeksi yang muncul selama penderita tersebut dirawat dirumah sakit dan mulai menunjukkan suatu gejala selama seseorang itu dirawat atau setelah selesai dirawat. Pasien yang berada di ruang ICU dapat mempunyai kecenderungan terkena infeksi nosokomial. Penelitian ini bertujuan untuk mengidentifikasi dan mengetahui bakteri aerob yang terbanyak penyebab infeksi nosokomial di ruang ICU RSAD Robert Wolter Mongisidi Teling Manado. Desain penelitian ini bersifat deskriptif dengan studi cross sectional. Sampel penelitian ialah bakteri pada lantai, dinding, udara, tempat tidur, dan EKG di ruang ICU RSAD Robert Wolter Mongisidi Teling Manado. Hasil penelitian memperlihatkan dari 26 sampel terdapat 26 sampel yang tumbuh yaitu, Bacillus sp. sebanyak delapan sampel (30,7%), Enterobacter aerogens ditemukan sebanyak enam sampel (23%), Staphylococcus sp. Ditemukan sebanyak enam sampel (23%) sedangkan Enterobacter cloacae, Serratia liquefaciens, Serratia rubidaea, Serratia marcescens, Klebsiella pneumoniae, Proteus sp. masing-masing ditemukan sebanyak satu sampel (3,8%). Simpulan: bakteri yang paling banyak ditemukan adalah Bacillus sp. Kata kunci: infeksi nosokomial, ICU, bakteri aerob

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