scholarly journals AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAN n-HEKSANA DARI DAUN RUMPUT SANTA MARIA (Artemisia vulgaris L.) PADA MINYAK IKAN

2014 ◽  
Vol 14 (2) ◽  
pp. 129
Author(s):  
Erpi Bangol ◽  
Lidya I. Momuat ◽  
Jemmy Abidjulu
Keyword(s):  
Antioxidant Activity ◽  
Fish Oil ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Artemisia Vulgaris ◽  
Leaves Of Grass ◽  
Hexane Extracts ◽  
Santa Maria

ABSTRAK Penelitian ini bertujuan untuk menentukan kandungan total fenolik dan aktivitas antioksidan ekstrak etanol dan n-heksana dari daun Artemisia vulgaris L. Total fenolik diukur menggunakan metode Follin-Ciocalteau dengan asam galat sebagai larutan standar. Aktivitas antioksidan diukur berdasarkan analisis kadar peroksida dan malonaldehida (MDA) dari minyak ikan. Hasil penelitian menunjukkan bahwa ekstrak etanol memiliki kandungan total fenolik sebesar 81.22 mg/kg dan ekstrak n-heksana sebesar 20.10 mg/kg. Kemampuan penghambatan peroksida maupun MDA oleh ekstrak etanol lebih tinggi daripada ekstrak n-heksana. Sebagai pembanding, α-tokoferol memiliki aktivitas penghambatan peroksida dan MDA lebih tinggi daripada ekstrak n-heksana, tetapi lebih rendah daripada ekstrak etanol. Kata kunci :Artemisia vulgaris L., antioksidan, fenolik, peroksida, malonaldehida   ANTIOXIDANT ACTIVITY OF THE ETHANOL AND n-HEXANE EXTRACTS OF LEAVES OF GRASS SANTA MARIA (Artemisia vulgaris L.) IN FISH OIL ABSTRACT The purpose of the study was to determine the total phenolic content and antioxidant activity of the ethanol and n-hexane extracts of leaves of Artemisia vulgaris L. Total phenolic was determined using Follin-Ciocalteau method with gallic acid as a standard solution. The antioxidant activity was measured based on the analysis of the levels of peroxide and malonaldehida (MDA) in fish oil. The results showed that the ethanol extract had a total phenolic content of 81.224 mg/kg and n-hexane extracts of 20.101 mg/kg. The ability of peroxide and MDA inhibition by ethanol extract was higher than that by n-hexane extract. For comparison, α-tocopherol has higher inhibitory activity on peroxide and MDA than n-hexane extracts, but lower than ethanol extract. Keywords: Artemisia vulgaris L., antioxidants, phenolic, peroxide, malonaldehida

scholarly journals AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAN n-HEKSANA DARI DAUN RUMPUT SANTA MARIA (Artemisia vulgaris L.) PADA MINYAK IKAN

2014 ◽  
Vol 14 (2) ◽  
pp. 129 ◽  
Author(s):  
Erpi Bangol ◽  
Lidya I. Momuat ◽  
Jemmy Abidjulu
Keyword(s):  
Antioxidant Activity ◽  
Fish Oil ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Artemisia Vulgaris ◽  
Leaves Of Grass ◽  
Hexane Extracts ◽  
Santa Maria

ABSTRAK Penelitian ini bertujuan untuk menentukan kandungan total fenolik dan aktivitas antioksidan ekstrak etanol dan n-heksana dari daun Artemisia vulgaris L. Total fenolik diukur menggunakan metode Follin-Ciocalteau dengan asam galat sebagai larutan standar. Aktivitas antioksidan diukur berdasarkan analisis kadar peroksida dan malonaldehida (MDA) dari minyak ikan. Hasil penelitian menunjukkan bahwa ekstrak etanol memiliki kandungan total fenolik sebesar 81.22 mg/kg dan ekstrak n-heksana sebesar 20.10 mg/kg. Kemampuan penghambatan peroksida maupun MDA oleh ekstrak etanol lebih tinggi daripada ekstrak n-heksana. Sebagai pembanding, α-tokoferol memiliki aktivitas penghambatan peroksida dan MDA lebih tinggi daripada ekstrak n-heksana, tetapi lebih rendah daripada ekstrak etanol. Kata kunci :Artemisia vulgaris L., antioksidan, fenolik, peroksida, malonaldehida   ANTIOXIDANT ACTIVITY OF THE ETHANOL AND n-HEXANE EXTRACTS OF LEAVES OF GRASS SANTA MARIA (Artemisia vulgaris L.) IN FISH OIL ABSTRACT The purpose of the study was to determine the total phenolic content and antioxidant activity of the ethanol and n-hexane extracts of leaves of Artemisia vulgaris L. Total phenolic was determined using Follin-Ciocalteau method with gallic acid as a standard solution. The antioxidant activity was measured based on the analysis of the levels of peroxide and malonaldehida (MDA) in fish oil. The results showed that the ethanol extract had a total phenolic content of 81.224 mg/kg and n-hexane extracts of 20.101 mg/kg. The ability of peroxide and MDA inhibition by ethanol extract was higher than that by n-hexane extract. For comparison, α-tocopherol has higher inhibitory activity on peroxide and MDA than n-hexane extracts, but lower than ethanol extract. Keywords: Artemisia vulgaris L., antioxidants, phenolic, peroxide, malonaldehida

scholarly journals Determination of phenolic contents and antioxidant activity test of ethanol extract of Sirih merah (Piper crocatum Ruiz & Pav.) leaves using the DPPH method

2021 ◽  
Vol 13 (2) ◽  
pp. 137-142
Author(s):  
Kasta Gurning ◽  
◽  
Sifikal Lumbangaol ◽  
Risanti F. R. Situmorang ◽  
Saronom Silaban ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Extraction Process ◽  
Total Phenolic ◽  
Betel Leaf ◽  
Dpph Method

The research objectives were to identify the secondary metabolite components, total phenolic content and determine the antioxidant activity of the ethanol extract of red betel leaf (Piper crocatum Ruiz & Pav.). The extraction process was carried out by materation using ethanol as a solvent. Determination of total phenolic content was carried out colorimetrically with Folin-Ciocalteu reagent measured at a maximum wavelength of 765 nm. Determination of antioxidant activity using the DPPH method measured by spectrophotometry at a maximum wavelength of 517 nm. The results of phytochemical screening of the ethanolic extract of red betel leaf contain secondary metabolites, including flavonoid, phenolic, tannin, alkaloids, steroids, and triterpenoids. The total phenolic content of the red betel leaf ethanol extract was 0.949±0.003 mg GAE/g d.w. and has antioxidant activity (IC50) 84,656 including strong category as an antioxidant. Keywords: Piper crocatum Ruiz & Pav., Antioxidant, Ethanol extract, Folin-Ciocalteu and DPPH

scholarly journals KORELASI KANDUNGAN FENOLIK DAN AKTIVITAS ANTIOKSIDAN DAUN JAMBU METE

Biomedika ◽  
2011 ◽  
Vol 3 (2) ◽  
Author(s):  
Ika Trisharyanti Dian Kusumowati ◽  
Rosita Melannisa ◽  
Kartikaning Ratri
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Anacardium Occidentale ◽  
Total Phenolic ◽  
Positive Correlation

The ethanolic extract of Anacardium occidentale L. leaves contains phenolic compounds,  avonoids, alkaloids, saponins, tannins, and steroids that could have antioxidant activity. The aim of this research was to determine the correlation between the antioxidant activity and the phenolic compounds of the ethanol extract of Anacardium occidentale L. leaf. The DPPH methode was used to determine the antioxidant activity. The total phenolic determined using Folin-Ciocalteu reagent. There was a positive correlation between antioxidant activity and total phenolic content in the ethanolic extract of cashew leaf with correlation coef cient R2 = 0.5888.Keywords: Anacardium occidentale L., antioxidant, phenolic compounds

scholarly journals Antioxidant activity, total phenolic and flavonoid content of several indigenous species of ferns in East Kalimantan, Indonesia

2019 ◽  
Vol 20 (2) ◽  
pp. 576-580
Author(s):  
HENNY NURHASNAWATI ◽  
REKSI SUNDU ◽  
SAPRI SAPRI ◽  
RISA SUPRININGRUM ◽  
HARLINDA KUSPRADINI ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Colorimetric Method ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Indigenous Species ◽  
Flavonoid Content ◽  
East Kalimantan ◽  
Phenolic And Flavonoid

Abstract. Nurhasnawati H, Sundu R, Sapri, Supriningrum R, Kuspradini H, Arung ET. 2019. Antioxidant activity, total phenolic and flavonoid content of several indigenous species of ferns in East Kalimantan, Indonesia. Biodiversitas 20: 576-580. This study aimed to determine the total phenolic and flavonoids content and antioxidant activity of ethanol extract of several indigenous species of ferns in East Kalimantan. Total phenolic content was determined by Folin-Ciocalteau method and flavonoid content was measured by colorimetric method. Antioxidant activity was evaluated by DPPH (2,2-diphenyl-1-picrylhydrazyl) method. The result of photochemical screening indicated that the leaves of Plagiogyria pycnophylla, Plagiogyria glauca, and Stenochlaena palustris contained alkaloid, flavonoid, tannin, saponin, and steroid while Acrostichum aureum contained flavonoid, tannin, saponin and steroid. The result showed extract Acrostichum aureum had the highest total phenolic content (366.4573 ± 2.2117 mg GAE.g-1), flavonoid content (228.6087 ± 2.2548 mg QE.g-1), and very strong antioxidant activity with IC50 value 29.5303 ppm. There is positive correlation between total phenolic content, flavonoid with antioxidant activity.

scholarly journals Total phenolic content and antioxidant activity of ginger extract and SNEDDS with eel fish bone oil (Anguilla spp.)

2018 ◽  
Vol 10 (3) ◽  
pp. 164-169
Author(s):  
IMAS IFRIAN WIJAYANTI ◽  
AGUNG BUDIHARJO ◽  
ARTINI PANGASTUTI ◽  
FEA PRIHAPSARA ◽  
ANIF NUR ARTANTI
Keyword(s):  
Antioxidant Activity ◽  
Fish Oil ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Fish Bone ◽  
Total Phenolic ◽  
Ginger Extract ◽  
Sod Activity ◽  
Treatment Groups

Wijayanti II, Budiharjo A, Pangastuti A, Prihapsara F, Artanti AN. 2018. Total phenolic content and antioxidant activity of ginger extract and SNEDDS with eel fish bone oil (Anguilla spp.). Nusantara Bioscience 10: 164-169. Aims of this study were to measure phenolic content of ginger (Zingiber officinale) and to observe the comparison of antioxidant activity between pure ginger extract and ginger extract in SNEDDS (Self Nanoemulsifying Drug Delivery System) with bone of eel fish oil (Anguilla spp.) as a conductor. Analysis was done based on IC50 value using DPPH (2,2 diphenyl-1-picrylhydrazyl) method and activity of Superoxide Dismutase (SOD) in vivo using Adrenochrome Assay. Determination of total phenolic content of ginger extract was measured by UV/Vis Spectrophotometric using Folin-Ciocalteau reagents then the absorbances were measured at wavelength 756 nm. Calculation of IC50 DPPH of the ginger extract and SNEDDS were measured using spectrophotometer UV/Vis at 517 nm wavelength. Measurements of SOD was based on the ability of SOD inhibit spontaneous auto reduction of epinephrine using Adrenochrome Assay at 12 male Wistar rats that divided into 4 treatment groups with 3 replicates for each group. Total phenolic content of ginger extract from 3 replications were 4,8519 ± 0,037 µg/mL equivalent with gallic acid standard solutions (GAE) or 485,1847 ± 3,7045 mgGAE/100 g dry weight. Antioxidant scavenging activity by DPPH assay of ginger was higher than SNEDDS. IC50 of ginger was 385.4 mg/mL, and IC50 of SNEDDS was 428.4 mg/mL. The result of descriptive analysis of Kruskal Wallis of SOD activity assay indicated significant differences between treatment groups (P = 0.001 (P <0.05)). SOD activity test performed in vivo showed that ginger extract made into SNEDDS system with eel fish oil used as the conductor has a significant influence compared with ginger extract group.

scholarly journals PHYTOCHEMICAL PROFILING, ASSESSMENT OF TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID CONTENT, AND ANTIOXIDANT ACTIVITY OF ETHNOMEDICINAL PLANT, MEYNA SPINOSA FROM ASSAM

2019 ◽  
pp. 61-63
Author(s):  
REKHA BORA ◽  
SEEMA KHAKHALARY ◽  
TAPAN DUTTA
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Radical Scavenging Assay

Objective: The present study qualitative phytoconstituents examine the total phenol, total flavonoid content (TFC), and antioxidant efficiencies traditionally used plant, Meyna spinosa. Methods: Chemical profiling, estimation of total phenolic content (TPC), TFC, and antioxidant activity of ethanol extracts of M. spinosa have performed by applying standard protocols. Antioxidant activity of leaf and stem was evaluated by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay. TPC and TFC of the plant were assessed using Folin–Ciocalteu colorimetric and aluminum colorimetric assay, respectively. Results: The findings of the study exhibit that ethanol extract of M. spinosa is proved to be the presence of phytoconstituents (7/9) such as alkaloids, terpenoids, saponins, tannins, phytosterols, flavonoids, and phenolic compounds, while carbohydrate, fixed oils, and fats are unavailable. In addition, phenolic compositions of ethanol extract of leaf and stem; 93.21±2.93 and 54.33±0.69 mg gallic acid equivalents/g extract, respectively; TFCs of leaf and stem have recorded as 61.55±1.21 and 37.55±1.28 mg quercetin equivalents/g extract, respectively. Antioxidant efficiency of both leaf and stem is tested using DPPH radical scavenging assay as IC50 20.68±0.32 and 50.99±0.56 μg/ml, respectively. Conclusion: From the above results, it has concluded that the ethanol extract of the M. spinosa leaves and stems seizes rich phytoconstituents which can be applied in food technology, drug industries, ethnopharmacological fields, etc., for the development of healthiness and to battle against negative health consequences.

scholarly journals CONTRIBUTION TO THE PHYTOCHEMICAL STUDY AND CHEMICAL TESTS OF THE EXTRACTS OF Citrus limonium (LEMON) and Capsicum frutescens L. (CHILLI PEPPER)

2015 ◽  
Vol 1 ◽  
pp. 21
Author(s):  
Laiza Cristina Carlos Freire ◽  
Emanuel Isaias Mota Araújo ◽  
Francisca Ítala da Silva Paiva Valdevino ◽  
Francisco Felipe Maia da Silva ◽  
Leonardo Alcântara Alves ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Total Phenolic ◽  
Capsicum Frutescens ◽  
Chilli Pepper ◽  
Phytochemical Study ◽  
Natural Medicine

Use of natural medicine has been used by man since time immemorial, and this use has grown considerably in recent times. Thus, this study aims to make a comparator analysis regarding the phytochemical test, antioxidant activity and total phenolic content of the extracts of the leaves in ethanol and their respective fractions of Citrus limonium (lemon) and Capsicum frutescens L. (chilli pepper). To compare them was observed that ethanol extract of both plants showed the better antioxidant activity, Capsicum frutescens L. with 204.60 ppm and 99.07 ppm with Citrus limonium. With regard to the phenolic content of the hexane extract and lemon extract in ethanol pepper were those that showed a higher content. Overall, it was noted that Citrus limonium excelled both in relation to their antioxidant activity and the phenolic content.  

scholarly journals IN VITRO EVALUATION OF ANTIOXIDANT POTENTIAL AND TOTAL PHENOLIC CONTENT OF BARLERIA LONGIFLORA LEAF EXTRACTS

2019 ◽  
pp. 212-215
Author(s):  
JOTHIMUNIYANDI M ◽  
JAYACHITRA A
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Metal Chelating ◽  
Reducing Ability

Objective: The aim of this present study is to estimate the antioxidant potential found in the leaves of Barleria longiflora that belongs to the family Acanthacea. Methods: Antioxidant activity of six different solvent extracts system of B. longiflora leaves was assayed for 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical activity, scavenging of hydrogen peroxide (H2O2), ferric reducing antioxidant activity, metal chelating ability assay, and total reducing ability. Total phenolic content of the extracts was determined by Folin–Ciocalteu method. Results: The radical scavenging activity was evaluated by DPPH of ethanol extract at concentration of 100 μg/mg was found to be 56.5% followed by methanol 48.4%, whereas maximum scavenging of H2O2 was observed in ethanol 83.4% followed by chloroform 70.8%. Ethanolic extract of B. longiflora leaves showed the highest value in ferric reducing antioxidant power assay 74.8%, metal chelating activity 61.6%, and total reducing ability 0.76±0.02 when compared to the standard ascorbic acid. Conclusion: The results suggest that the antioxidant potential of the ethanol extract has the highest activity in compared to other five extracts of B. longiflora leaves.

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