scholarly journals UJI AKTIVITAS FAGOSITOSIS MAKROFAG EKSTRAK ETANOL DAUN SUJI (Dracaena angustifolia (Medik.)Roxb. ) SECARA IN VITRO

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Nestri Handayani
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Immunomodulatory Activity ◽  
Macrophage Activity ◽  
Macrophage Phagocytosis ◽  
Phagocytosis Index ◽  
Layer Chromatography

UJI AKTIVITAS FAGOSITOSIS  MAKROFAG  EKSTRAK ETANOL  DAUN SUJI (Dracaena angustifolia (Medik.)Roxb. ) SECARA  IN VITRONestri Handayani 1,2*), Subagus Wahyuono2), Triana Hertiani2), Retno Murwanti2)1)Program Studi Farmasi, FMIPA, Universitas Sebelas Maret Surakarta2)Fakultas Farmasi Universitas Gadjah Mada Yogyakarta*Email : [email protected]  ABSTRACT Immunomodulatory activity has been carried out on ethanol extract of suji leaf (Dracaena angustifolia (Medik.) Roxb with in vitro macrofag phagocytosis method. . Suji is a plant that is often used as a food coloring and has long been used medicinally. This study aimed to test in vitro macrophage phagocytosis on ethanol extract of suji leaf. The suji leaves are extracted by maceration method. The obtained viscous extract was then tested for Thin Layer Chromatography (TLC) and in vitro macrophage phagocytosis test. The parameters used are Phagocytosis Index and Macrophage Capacity. The results of in vitro macrophage phagocytosis test with 4 concentrations of 10.25,50 and 100 microgram / ml, showed for the largest average Fagocytosis (IF) Index shown at concentration 10 mcg / ml, while concentrations 25 and 50 mcg / ml showed values The same phagocytic (KF) capacity. Keywords :  Suji leaf, Phagocytic Macrophage Activity,  ethanolic  extract   ABSTRAKUji aktivitas imunomodulator telah dilakukan terhadap ekstrak etanol  daun suji (Dracaena angustifolia (Medik.)Roxb..  Suji merupakan tanaman yang sering digunakan sebagai pewarna makanan dan telah lama digunakan untuk obat. Penelitian ini bertujuan untuk melakukan uji fagositosis makrofag in vitro terhadap ekstrak etanol daun suji. Daun suji diekstraksi dengan metode maserasi. Ekstrak kental yang diperoleh kemudian dilakukan uji Kromatografi Lapis Tipis (KLT) dan uji fagositosis makrofag in vitro. Parameter yang digunakan adalah Indeks fagositosis dan Kapasitas makrofag. Hasil uji fagositosis makrofag in vitro dengan 4 konsentrasi sebesar 10,25,50 dan 100 mikrogram/ml, menunjukkan untuk Indeks Fagositosis (IF) rata-rata terbesar ditunjukkan pada konsentrasi 10 mcg/ml, sedangkan konsentrasi 25 dan 50 mcg/ml menunjukkan nilai  Kapasitas Fagositosis (KF) yang sama besar. Kata kunci : Daun suji, fagositosis makrofag, ekstrak etanol    

scholarly journals High performance thin-layer chromatography and in vitro cytotoxic studies on ethanol extract of Matricaria chamomilla L (Asteraceae) flowers

2021 ◽  
Vol 18 (9) ◽  
pp. 1969-1976
Author(s):  
Mohammed Al Bratty ◽  
Lalitha K. Govindaram ◽  
Lalitha K. Govindaram ◽  
Neelaveni Thangavel ◽  
Hassan A. Alhazmi ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mtt Assay ◽  
High Performance ◽  
Cytotoxic Effect ◽  
Ethanol Extract ◽  
Matricaria Chamomilla ◽  
Layer Chromatography ◽  
Mcf 7

Purpose: To develop a high performance thin-layer chromatography (HPTLC procedure for quantitation of apigenin in ethanol extract of Matricaria chamomilla (Babunaj) flowers, and to evaluate the extract for in vitro cytotoxic effect on MCF-7 cell lines. Methods: Quantification of apigenin was carried out using a CAMAG TLC system. A combination of toluene, ethyl acetate and formic acid (4.5:3.5:0.2 v/v/v) was used as mobile phase, with densitometry detection at 336 nm. The HPTLC procedure was subjected to validation as per ICH guidelines. The cytotoxicity of the extract was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Results: A sharp apigenin band at Rf of 0.51 was obtained, and the content of apigenin in the extract was 0.062 % w/w. The detection limit (LOD) and quantification limit (LOQ) were 0.19 and 0.57 ng/band, respectively. MTT assay results indicate that M. chamomilla was cytotoxic to Michigan Cancer Foundation-7 (MCF-7) cells, with half-maximal concentration (IC50) of 74 µg/mL. Conclusion: The developed HPTLC method is linear, precise, accurate and specific for the determination of apigenin. M. chamomilla exerts cytotoxic effect on MCF-7 cell line via induction of apoptosis.

Phytochemical, Analytical and Medicinal Studies of Holoptelea integrifolia Roxb. Planch - A Review

2019 ◽  
Vol 5 (4) ◽  
pp. 270-277 ◽  
Author(s):  
Vijay Kumar ◽  
Simranjeet Singh ◽  
Ragini Bhadouria ◽  
Ravindra Singh ◽  
Om Prakash
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Biologically Active ◽  
Toxicological Studies ◽  
Wide Range ◽  
Layer Chromatography

Holoptelea integrifolia Roxb. Planch (HI) has been used to treat various ailments including obesity, osteoarthritis, arthritis, inflammation, anemia, diabetes etc. To review the major phytochemicals and medicinal properties of HI, exhaustive bibliographic research was designed by means of various scientific search engines and databases. Only 12 phytochemicals have been reported including biologically active compounds like betulin, betulinic acid, epifriedlin, octacosanol, Friedlin, Holoptelin-A and Holoptelin-B. Analytical methods including the Thin Layer Chromatography (TLC), High-Performance Thin Layer Chromatography (HPTLC), High-Performance Liquid Chromatography (HPLC) and Liquid Chromatography With Mass Spectral (LC-MS) analysis have been used to analyze the HI. From medicinal potency point of view, these phytochemicals have a wide range of pharmacological activities such as antioxidant, antibacterial, anti-inflammatory, and anti-tumor. In the current review, it has been noticed that the mechanism of action of HI with biomolecules has not been fully explored. Pharmacology and toxicological studies are very few. This seems a huge literature gap to be fulfilled through the detailed in-vivo and in-vitro studies.

scholarly journals Profile of Thin-Layer Chromatography and UV-Vis Spectrophotometry of Akar Kuning Stem Extract (Arcangelisia flava)

2018 ◽  
Vol 1 (2) ◽  
pp. 72-76 ◽  
Author(s):  
Mohammad Rizki Fadhil Pratama ◽  
Suratno Suratno ◽  
Evi Mulyani
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Ethanol Extract ◽  
Retention Factor ◽  
Polar Solvents ◽  
Central Kalimantan ◽  
Stem Extract ◽  
Rf Values ◽  
Layer Chromatography ◽  
Chloroform Methanol

This study aims to obtain the profile of Thin-Layer Chromatography (TLC) and Ultraviolet-Visible (UV-Vis) spectrophotometry from ethanol extract of akar kuning stems (Arcangelisia flava) from Central Kalimantan. The TLC method is used with the orientation phase of the combination of polar-non-polar solvents resulting from orientation, while ethanol is used as the solvent for UV-Vis spectrophotometers. TLC results showed the formation of 3 stains on a combination of polar solvents chloroform : methanol : water while in a non-polar solvent combination n-hexane : ethyl acetate did not show any stains. Comparison of retention factor (Rf) values show the best combination of polar solvents to separate stains at a ratio of 5 : 2 : 1, respectively. Separation in 2-dimensional TLC with polar solvents showed a similar pattern with 1-dimensional separation in the form of 3 stains. UV-Vis spectrophotometer results showed 4 main peaks with wavelength 227.2; 267.4; 345.2; and 425.3 nm, respectively. The profile of the peak formed is very similar to that shown by berberine, one of the main metabolites of akar kuning. TLC and UV-Vis spectrophotometers profiles obtained are expected to support further research using akar kuning stems, especially those from Central Kalimantan.

scholarly journals The phytochemical screenings and thin layer chromatography analysis of chemical compounds in ethanol extract of labu siam fruit (Sechium edule Jacq. Swartz.)

2005 ◽  
Vol 3 (1) ◽  
pp. 26-31 ◽  
Author(s):  
SOERYA DEWI MARLIANA ◽  
VENTY SURYANTI ◽  
SUYONO SUYONO
Keyword(s):  
Thin Layer ◽  
Petroleum Ether ◽  
Thin Layer Chromatography ◽  
Soxhlet Extraction ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Thin Layer Chromatography Analysis ◽  
Chromatography Analysis ◽  
Sechium Edule ◽  
Layer Chromatography

The phytochemical screenings and analysis of chemical compounds in ethanol extract of labu siam fruit (Sechium edule Jacq. Swartz.) with Thin Layer Chromatography (TLC) has been carried out. Isolation was done by Soxhlet extraction for 6 hours with petroleum ether and the residue was extracted by maceration during 24 hours with ethanol.The isolated compounds in ethanol extract were identified by phytochemical screenings method and TLC. The result showed the presence of alkaloid, saponin, cardenolin/bufadienol and flavonoid.

THE ACTION OF DIBUTYRYL CYCLIC 3′,5′-ADENOSINE MONOPHOSPHATE ON THE MOUSE THYROID GLAND IN VITRO

1970 ◽  
Vol 47 (3) ◽  
pp. 333-338 ◽  
Author(s):  
PAT KENDALL-TAYLOR ◽  
D. S. MUNRO
Keyword(s):  
Thyroid Gland ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Adenosine Monophosphate ◽  
Thyroid Stimulating Hormone ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Layer Chromatography ◽  
Mouse Thyroid

SUMMARY The effects of dibutyryl cyclic 3′,5′-adenosine monophosphate (DBc-AMP) on the mouse thyroid gland have been investigated in the in-vitro assay of Brown & Munro (1967). The distribution of 131I-labelled compounds in the glands and the supporting medium have been analysed by thin-layer chromatography and the changes induced by cyclic 3′,5′-adenosine monophosphate (c-AMP), DBc-AMP or thyroid-stimulating hormone (TSH) compared. The release of 131I was increased when the glands were incubated with DBc-AMP, c-AMP or TSH. The potency of DBc-AMP was approximately 50 times that of c-AMP on a basis of molarity. Like TSH, DBc-AMP increased the proportion of iodothyronines in the system as a whole, whereas c-AMP had little effect. The possible explanations for this are discussed.

scholarly journals UJI TOKSISITAS DAN IDENTIFIKASI SENYAWA EKSTRAK ALGA MERAH (Eucheuma cottonii) TERHADAP LARVA UDANG Artemia salina LEACH

ALCHEMY ◽  
2013 ◽  
Author(s):  
Noer Mardlatillah Sharo ◽  
Rachmawati Ningsih ◽  
Ahmad Hanapi ◽  
Ahmad Nasichuddin
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Red Algae ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Active Compounds ◽  
Layer Chromatography ◽  
Eucheuma Cottonii

<p><em>Eucheuma cottonii</em> is a multicellular algae (multicellular) that have bioactive compounds used in medicine, such as anticancer. The purpose of this research is to know the level of toxicity of the crude extract of ethanol and n-hexane red algae (<em>Eucheuma cottonii</em>) against larvae of shrimp <em>A</em><em>. salina</em> Leach and to know the active compounds contained in crude extracts of ethanol and n-hexane red algae (<em>Eucheuma cottonii</em>).</p><p>This research was conducted with the maceration extraction continuing by test toxicity against the shrimp larvae of <em>A</em><em>. salina</em> Leach (BSLT). The result of the toxicity test then proceeds with the test reagents and phytochemicals with thin layer chromatography for positive test of compound phytochemicals. Thin-layer chromatography using eluen n-hexane: ethyl acetate (7: 3) to ethanol extract and n-hexane: ethyl acetate (17: 3) for n-hexane extract. Data on toxicity of <em>A. salina Leach</em><em> </em>analyzed by probit analysis to find out the value of LC<sub>50</sub> on each extract.</p><p>The results of this research indicate that each of the rough red algae extract (<em>Eucheuma cottonii</em>) by using a solvent of ethanol and n-hexane have toxicity values (LC<sub>50</sub>) of shrimp larvae of <em>A</em><em>. salina</em> Leach consecutive 58,0128 ppm and 61,7571 ppm. Active compounds content of Phytochemical test results i.e. triterpenoid (ethanol extract) and steroids (n-hexsane extract). The TLC triterpenoid obtained 7 stains, and the results obtained steroids 8 TLC stains.</p>

Sign in / Sign up

Export Citation Format

Share Document