scholarly journals Evaluation of the effect of probiotic bacteria on growth performance and survival rate of carp, Cyprinus carpio

2019 ◽  
Vol 7 (1) ◽  
pp. 243
Author(s):  
Jeane T Sumaraw ◽  
Henky Manoppo ◽  
Reiny A. Tumbol ◽  
I.F.M Rumengan ◽  
Henny A. Dien ◽  
...  
Keyword(s):  
Body Weight ◽  
Survival Rate ◽  
Growth Performance ◽  
Cyprinus Carpio ◽  
Probiotic Bacteria ◽  
Pathogenicity Test ◽  
Feeding Frequency ◽  
Biochemical Tests ◽  
Growth Performances ◽  
Carp Cyprinus Carpio

The objective of this research was to obtain probiotic bacteria isolated from the digestive tract of catfish Clarias batracus, to evaluate its effect on growth performances and survival rate of carp. After purified, the bacteria was identified through biochemical tests and selection was carried out through proteolytic, amylolytic, cellulolytic, tests as well as pathogenicity test. Probiotic candidate was administered orally to fish at different concentrations. The fish were fed for 30 days respectively as much as 3% of body weight per day with feeding frequency of two times a day. The results showed that addition of probiotics into feed significantly increased growth performance but not survival rate.Keywords: Probiotic, Clarias batracus,  growth performance, aquaculture. ABSTRAKTujuan dari penelitian ini adalah untuk mendapatkan bakteri probiotik yang diisolasi dari saluran pencernaan ikan lele Clarias batracus, dan mengevaluasi pengaruhnya terhadap kinerja pertumbuhan dan kelangsungan hidup benih ikan mas. Bakteri probiotik diidentifikasi melalui uji biokimia dan seleksi dilakukan melalui proteolitik, amilolitik, tes selulolitik dan patogenisitas. Kandidat probiotik diberikan pada ikan secara oral dengan konsentrasi berbeda Pemberian pakan berprobiotik berlangsung selama 30 hari sebanyak  3% dari berat badan per hari dengan frekuensi pemberian dua kali sehari. Hasil penelitian menunjukkan bahwa penambahan probiotik ke dalam pakan secara signifikan meningkatkan kinerja pertumbuhan namun tidak memengaruhi kelangsungan hidup benih ikan mas.Kata kunci : Probiotik, Clarias batracus, kinerja pertumbuhan, budidaya

scholarly journals ADDITION OF POTENTIAL PROBIOTIC BACTERIA TO INCREASE GROWTH PERFORMANCE AND FEED EFFICIENCY OF COMMON CARP (Cyprinus carpio)

2017 ◽  
Vol 11 (1) ◽  
pp. 31-34
Author(s):  
Cut Yulvizar ◽  
Irma Dewiyanti ◽  
Cut Nanda Defira ◽  
T.M. Iqbalsyah ◽  
Suhartono Suhartono
Keyword(s):  
Survival Rate ◽  
Growth Performance ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Feed Efficiency ◽  
Intestinal Bacteria ◽  
Probiotic Bacteria ◽  
Daily Growth ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio

Three intestinal bacteria isolates (namely C1, C2, and C3) from common carp (Cyprinus carpio) were isolated and identified as new potential probiotics. This research aimed to study the effect of three isolates potential probiotics addition on growth performance, survival rate and feed efficiency of common carp. Completely randomized design was assigned to fourth diet treatments with five replicates. As a control, we used the feed without any supplemention of potential probiotic. The result showed that the addition of potential probiotic bacteria (C1, C2, and C3) gave significant effect on daily growth rate, survival rate and feed efficiency and could be used in aquaculture industry to reduce feeding cost.

scholarly journals Evaluation of Hatchability from Various Intraspecific Crosses of Carp and Effect of Frequencies of Feeding on Stocking Density of Common Carp Fry

2019 ◽  
Vol 1 ◽  
pp. 88-95
Author(s):  
O O Jacob ◽  
G S Solomon ◽  
P A Annune
Keyword(s):  
Body Weight ◽  
Single Dose ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Stocking Density ◽  
Fertilization Rate ◽  
Feeding Frequency ◽  
Male And Female ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio

Common carp (Cyprinus carpio) germplasm were sourced from Bauchi and Ibadan aimed at studying their hatchability from the various intraspecific crosses and effect of frequencies of feeding on stocking density of Carp fry. Both male and female were injected with Ovaprim at 0.5ml/kg body weight intramuscularly at a single dose. The number of eggs in 1 gram mass was determined, Fertilization rate and percentage of hatchability were calculated. One hundred and eighty (180) fry of Cyprinus carpio were randomly picked and distributed in nine bowls of 50 litres at three stocking densities with replicates for each treatment. The frequencies of feeding were in these order; Ten (10) fry per bowl of 50 litres were fed twice a day, Twenty ( 20) fry per bowl of 50 litres were fed four times a day, Thirty (30) fry per bowl of 50 litres were fed six times a day, respectively for 8 weeks with 30gm of 0.2mm Coppens starter diet. The Ib♀ x Ba♂ cross had the highest hatchability though highest fertilization rate was recorded by Ba♀ X Ba♂ cross. The various crosses had above 50% hatchability, though percentagehatchability differed significantly at (P <0.05). While the growth pattern of the different stocking densities at different feeding frequencies differed significantly (p<0.05). The intra specific crossing of different sexes from the two sources gave a higher yield as can be seen with the IB♀ x BA♂. Whereas carp fry stocked at a lower stocking density with a fewer feeding frequency had best growth.

scholarly journals Effects of feeding frequency on growth performances and RNA : DNA ratio of the gangetic mystus (Mystus cavasius Hamilton, 1822) in laboratory condition

2018 ◽  
Vol 27 (1) ◽  
pp. 75-83
Author(s):  
Mosheur Rahman ◽  
Mohammad Shamimul Alam ◽  
Md Golam Rabbane
Keyword(s):  
Survival Rate ◽  
Growth Performance ◽  
Laboratory Condition ◽  
Condition Factor ◽  
Feeding Frequency ◽  
Laboratory Rearing ◽  
Laboratory Facilities ◽  
Growth Performances ◽  
Significant Difference ◽  
Rna:Dna Ratio

Experiments were conducted for a period of 90 days to estimate the effects of different feeding frequencies (one time per day: T1, two times per day: T2, three times per day:T3 and four times per day: T4) on growth performance of gulsha fish reared in laboratory facilities. Significantly higher condition factor was observed in T2 (1.61 ± 0.02) and lowest in T4 (1.40 ± 0.01). Significantly lowest SGR and ADG were recorded in T4 fish fed four times per day. No significant difference was obtained among different treatments for FCR and survival rate. The highest RNA : DNA ratio (0.93 ± 0.07) was observed in fish of T2 and the lowest ratio (0.57 ± 0.11) was observed in fish of T1. The results of the present study suggest that in laboratory rearing two meals per day could be supplied for better growth performances as per indication of condition factor and RNA:DNA ratio. Dhaka Univ. J. Biol. Sci. 27(1): 75-83, 2018 (January)

scholarly journals Isolasi bakteri probiotik dari organ pencernaan ikan mas (Cyprinus carpio)

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Sintia S. Bella
Keyword(s):  
Cyprinus Carpio ◽  
Challenge Test ◽  
Probiotic Bacteria ◽  
Pathogenicity Test ◽  
Gram Stain ◽  
Biochemical Tests ◽  
Gram Positive ◽  
Fish Hatchery ◽  
Digestive Organs ◽  
Probiotic Lactobacillus

The study aimed to isolate probiotic bacteria from the digestive organs of goldfish taken from Fish Hatchery, Tateli.  As much as 1 g of the intestine was crushed, put in 9 mL NaCl and centrifuged twice at 1000 rpm for 10 minutes. The supernatant was removed and diluted at a concentration of 10-2 - 10-3, then spread on the surface of the MRS media. The bacteria were incubated at 280C for 24-48 hours in the Incubator. The Gram Stain test found that the isolated bacterium was gram positive, biochemical tests found that bacterium isolated from the gut of carp was Lactobacillus sp. while pathogenicity test found that there was no mortality of fish until the 14th day after the challenge test.  So that it could be concluded that the bacteria obtained could be used as probiotics.Keywords:  probiotic, Lactobacillus sp., Gram Stain, pathogenesity

scholarly journals The Effectiveness of Candidate Probiotic Bacteria to Control Vibriosis Disease

2017 ◽  
Vol 5 (2) ◽  
pp. 1
Author(s):  
Mulyati Mulyati ◽  
Suryati Suryati ◽  
Irfani Baga
Keyword(s):  
Survival Rate ◽  
Sea Water ◽  
Challenge Test ◽  
Probiotic Bacteria ◽  
Pathogenicity Test ◽  
Inhibitory Ability ◽  
Significant Difference ◽  
Portunid Crab

The study aims to isolate, characterize, and examine probiotic bacteria's inhibitory ability against Vibrio harveyi bacteria, both in-vitro and in vivo. Methods used in the study consist of 1) An Isolation of Candidate Probiotic Bacteria, 2) An Antagonistic Test of Candidate Probiotic Bacteria in vitro, 3) An Identification of Bacteria, 4) A Pathogenicity Test of Candidate Probiotic Bacteria, 5) An Antagonistic Test of Candidate Probiotic Bacteria against V. harveyi in vivo. According to the isolation of candidate probiotic bacteria, there are 18 isolated candidate probiotic. After being tested for its inhibitory ability in vitro, there are 8 isolates with zone of inhibition as follows: isolate MM 7 from intestine (22 mm), isolate MM 6 from intestine (12 mm), isolate MM 10 from sea water (10 mm), isolate MM 5 from intestine (9 mm), isolate MM 4 from intestine (8 mm), isolate MM 3 from intestine (7 mm), isolate MM 2.2 from intestine (7 mm), isolate MM 2.1 from intestine (7 mm). Eight genera of the candidate probiotic bacteria is derived from Portunid crab, they are Staphylococcus, Streptococcus, bacillus, vibrio, Alcaligenes, Lactobacillus, micrococcus. Before proceeding the V. harveyi bacterial challenge test in vivo, three potential isolates consisting of MM6, MM7 and MM10 as the probiotic bacteria are pathogenicity-tested against V. harveyi. The survival rate of Portunid crab on pathogenicity test using MM6, MM7 and MM10 generates 91.11-100%, while the control generates 100% survival rate. Variance analysis result through post-hoc Tukey's Honest Significant Difference (HSD) test at 95% confidence interval indicates that isolate MM7 and MM10 are significantly able to increase hatchling Portunid crab's survival rate.

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