ISOLASI DAN SKRINING AKTIVITAS ANTIBAKTERI DARI BAKTERI SIMBION NUDIBRANCHIA Phyllidiella pustulosa dan Thuridilla lineolata

2020 ◽  
Vol 8 (2) ◽  
pp. 40
Author(s):  
Ade Kurniawan Arie ◽  
Rosita A J Lintang ◽  
Remy E P Mangindaan ◽  
Agung B Windarto ◽  
Fitje Losung ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Symbiotic Bacteria ◽  
Bacterial Isolates ◽  
Defense Systems ◽  
Self Defense ◽  
Solid Media ◽  
Antibacterial Assay ◽  
And Staphylococcus Aureus

Nudibranchs are marine invetebrates belongs to the class of Gastropoda that are able to camouflage and develop a self-defense systems. Marine bacteria contain chemicals compunds that have potentials on marine drugs discovery through the secondary metabolism. The purpose of this study was to isolate the symbiotic bacteria from nudibranches Phylidiella pustulosa and Thuridilla lineolate and to screen the antibacterial activity of these bacterial isolates against Escherichia coli and Staphylococcus aureus. Isolation and culture of the symbiotic bacteria was made on B1 (solid) media. The results of the antibacterial assay showed that the symbiotic bacterial isolates from Phylidiella pustulosa and Thuridilla lineolate were able to inhibit the growth of the Staphylococcus aureus and Escherichia coli test. Keywords: nudibranch, Phyllidiella pustulosa, Thuridilla lineolate, bacteria, antibacterialAbstrakNudibranch adalah avetebrata laut dalam kelas Gastropoda yang mampu melakukan kamuflase dan mengembangkan sistem pertahanan diri. Bakteri laut mengandung senyawa kimia yang berpotensi sebagai bahan obat-obatan yang dihasilkan melalui metabolit sekunder. Tujuan dari penelitian ini yaitu untuk mengisolasi bakteri yang bersimbion dengan nudibranch Phylidiella pustulosa dan Thuridilla lineolate, kemudian mengamati aktivitas antibakteri dari isolat bakteri tersebut terhadap Escherichia coli danStaphylococcus aureus. Isolasi dan kultur bakteri yang bersimbion dibuat pada media B1 (padat). Skrining aktivitas antibakteri menunjukkan isolat bakteri yang bersimbion dengan nudibranch Phylidiella pustulosa dan Thuridilla lineolate mampu menghambat pertumbuhan organisme uji Staphylococcus aureus dan Escherichia coli. Kata kunci: nudibranch, Phyllidiella pustulosa, Thuridilla lineolate, bakteri, antibakteri

scholarly journals ISOLASI BAKTERI YANG BERSIMBION DENGAN ASCIDIAN Herdmania momus YANG MEMILIKI AKTIVITAS ANTIBAKTERI

2020 ◽  
Vol 8 (2) ◽  
pp. 20
Author(s):  
Silvia E Koyongian ◽  
Deiske A Sumilat ◽  
Rosita A J Lintang ◽  
Stenly Wullur ◽  
Sandra O Tilaar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Marine Invertebrates ◽  
Nutrient Agar ◽  
Symbiotic Bacteria ◽  
Bacterial Isolates ◽  
Marine Broth ◽  
Antibacterial Screening ◽  
Coral Reef Ecosystems ◽  
And Staphylococcus Aureus

 Ascidian is marine invertebrates in coral reef ecosystems that produce many bioactive compounds for pharmacology. The presence of symbiotic bacteria with marine organisms is protected the host biota by producing secondary metabolites. The purpose of this study is to obtain symbiotic bacterial isolates with Herdmania momus ascidian, then to observe the antibacterial activity of these bacterial isolates against Escherichia coli, and Staphylococcus aureus. Isolation and culture of the symbiotic bacteria were made on Nutrient Agar and Zobell Marine Broth media. The antibacterial screening showed that the Herdmania momus symbiotic bacteria were able to inhibit the growth of Staphylococcus aureus and Escherichia coli.Keywords: ascidians, Herdmania momus, bacteria, isolation, antibacterialAbstak          Ascidian adalah avetebrata laut di ekosistem terumbu karang yang banyak menghasilkan senyawa bioaktif untuk bidang farmakologi. Keberadaan bakteri yang bersimbion dengan organisme laut pada umumnya untuk melindungi biota yang ditumpanginya dan dirinya dengan cara menghasilkan senyawa metabolit sekunder. Tujuan dari penelitian ini yaitu untuk mendapatkan isolat bakteri yang bersimbion dengan ascidian Herdmania momus, kemudian mengamati aktivitas antibakteri dari isolat bakteri tersebut terhadap Escherichia coli, dan Staphylococcus aureus. Isolasi dan kultur bakteri yang bersimbion dengan ascidian dibuat pada media Nutrient Agar dan Zobell Marine Broth. Skrining aktivitas antibakteri menunjukkan isolat bakteri yang bersimbion dengan ascidian Herdmania momus mampu menghambat pertumbuhan organisme uji Staphylococcus aureus dan Escherichia coli.Kata kunci: ascidian, Herdmania momus, bakteri, isolasi, antibakteri

scholarly journals Antibacterial Activity of Indian Spices Against Escherichia coli

2020 ◽  
Vol 2 (1) ◽  
pp. 13
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Inhibitory Effect ◽  
Zone Of Inhibition ◽  
Agar Gel ◽  
Antibacterial Assay ◽  
And Staphylococcus Aureus ◽  
Inhibition Technique ◽  
Gel Diffusion

The aim of the present investigation is to study the antibacterial activity of Indian spices such as clove, garlic, ginger, and two forms of tea that is a granular form of leaf and dust form of tea and green leaf tea. The antibacterial assay was done by collecting the concentrate of the spices, and the leaves extract against the test strain Escherichia coli and Staphylococcus aureus by agar gel diffusion inhibition technique. Since the zone of inhibition was observed in the case of all spices, clove, ginger, garlic showed a good inhibitory effect on Staphylococcus aureus and Tea extracts were effective on Escherichia coli.

Preparation, Characterization and Antibacterial Activity of Chitosan Schiffbases

2011 ◽  
Vol 287-290 ◽  
pp. 1947-1951 ◽  
Author(s):  
Jing Xian Xu ◽  
Ye Ting Lin ◽  
Qiang Lin ◽  
Wen Yuan ◽  
Xue Qiong Yin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Schiff Bases ◽  
Optical Density ◽  
Elemental Analysis ◽  
Antibacterial Activities ◽  
Cp Mas Nmr ◽  
And Staphylococcus Aureus ◽  
Better Than

Two Schiff bases of chitosan (CTS) were synthesized from 4-methoxylbenzylaldehyde (CH3O-CTS) and 4-methylbenzylaldehyde(CH3-CTS). The Schiff bases were characterized by FTIR, DSC-TGA, solid13C CP-Mas NMR, and elemental analysis. Antibacterial activities of the Schiff bases against Escherichia coli and Staphylococcus aureus were measured by the optical density method. The antibacterial activity of the Schiff bases is better than that of the original CTS. The IC50of CH3O-CTS and CH3-CTS againstEscherichia coliis respectively 40.3 ppm and 43 ppm, which being 38.5 ppm and 39.5 ppm against Staphylococcus aureus, lower than IC50of chitosan, being 59.5 ppm and 52 ppm againstEscherichia coliand Staphylococcus aureus, respectively.

scholarly journals Pharmacological Role of Anions (Sulphate, Nitrate, Oxalate and Acetate) on the Antibacterial Activity of Cobalt(II), Copper(II) and Nickel(II) Complexes With Nicotinoylhydrazine-Derived ONO, NNO and SNO Ligands

1996 ◽  
Vol 3 (5) ◽  
pp. 211-217 ◽  
Author(s):  
Zahid Hussain Chohan ◽  
Abdur Rauf
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Metal Ion ◽  
Bacterial Species ◽  
Biologically Active ◽  
Schiff Base Ligands ◽  
Mixed Ligands ◽  
And Staphylococcus Aureus

Mixed ligands biologically active complexes of cobalt(II), copper(II) and nickel(II) with nicotinoylhydrazine-derived ONO, NNO and SNO donor schiff-base ligands having the same metal ion but different anions such as sulphate, nitrate, oxalate and acetate have been synthesised and characterised on the basis of their physical, analytical and spectral data. In order to evaluate the role of anions on their bioability, these ligands and their synthesised metal complexes with various anions have been screened against bacterial species such as Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus and the title studies have proved a definative role of anions in increasing the biological activity

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