The Antibacterial Activity of Locally Grown Turmeric (Guyana) Using Ethanol Extracts at Different Concentrations against Escherichia Coli, Proteus Vulgaris, Pseudomonas Aeruginosa and Staphylococcus Aureus

2020 ◽  
Vol 7 (1) ◽  
pp. 8-18
Author(s):  
Nirvanie Persaud ◽  
Paulette Ragobeer ◽  
Ruth Daniel
2019 ◽  
Vol 12 (2) ◽  
Author(s):  
Gino Nemesio Cepeda ◽  
Meike Meilan Lisangan ◽  
Isak Silamba ◽  
Nitia Nilawati ◽  
Eka Syartika

ABSTRACT  Akway (Drimys piperita) is a woody, evergreen and aromatic plant that belongs to family winteraceae. This plant is used by Sougb tribe lived in Sururey village, District of Anggi, to enhance the vitality of body. The objectives of the research were to determine antimicrobial stability of akway bark extracts influenced by heating time of 100OC, levels of acidity (pH) and salt contents.. Antimicrobial assays were done by using agar well diffusion method against four species of bacteria, i.e.  Escherichia coli ATCC25922, Bacillus cereus ATCC10876, Pseudomonas aeruginosa ATCC27853 and Staphylococcus aureus ATCC25923. The results showed that ethanolic extracts of akway bark only inhibited growth of B. cereus and S. aureus with minimum inhibitory concentration 0,99% and 0,89% . The levels of concentration and acidity of ethanol extracts  influenced the antimicrobial capacity of extracts.. Whereas heating time on 100OC during 25 minutes and salt contents  up to 5% of extract solution did not influence the antimicrobial stability of  akway bark extracts. Key words : akway, extracts, antimicrobe, pH, Heating, salt ABSTRAK Akway (Drimys piperita) adalah tumbuhan berkayu, aromatik dan hijau sepanjang tahun dan tergolong dalam suku winteraceae. Tumbuhan ini digunakan oleh Suku Sougb yang bermukim di desa Sururey Distrik Anggi, untuk mengobati malaria dan meningkatkan vitalitas tubuh. Tujuan dari penelitian ini adalah untuk menentukan stabilitas antimikroba ekstrak kulit kayu akway pada waktu pemanasan ekstrak pada 100OC, tingkat keasaman (pH) dan kandungan garam. Pengujian antimikroba dilakukan dengan menggunakan metode difusi agar terhadap empat spesies bakteri yaitu Escherichia coli ATCC25922, Bacillus cereus ATCC10876, Pseudomonas aeruginosa ATCC27853 and Staphylococcus aureus ATCC25923. Hasil menunjukkan bahwa ekstrak etanol kulit kayu akway hanya dapat menghambat bakteri Bacillus cereus dan Staphylococcus aureus dengan konsentrasi hambat minimum masing-masing adalah 0,99% dan 0,89%. Tingkat konsentrasi dan keasaman (pH) mempengaruhi kapasitas antimikroba ekstrak etanol kulit kayu akway. Sedangkan perlakuan pemanasan pada suhu 100OC dengan lama pemanasan sampai dengan 25 menit dan penambahan garam NaCl sampai konsentrasi 5%  tidak berpengaruh pada stabilitas antimikroba ekstrak etanol kulit kayu akway.  


Author(s):  
Rajanikanth Garapati ◽  
N. Ramesh

Objective: In vitro investigated the potential of methanol extracts of micro-propagated C. orchiodes in the antimicrobial property against the three gram-negative bacteria, two gram-positive and one fungal filament.Methods: The micro propagated callus methanol extract was examined against Escherichia coli, Proteus vulgaris, Salmonella typhimurium, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus and Candida albicans. The zone of inhibitions are determined at 10 mg/ml concentration of methanol extracts of callus on agar well plate and MIC against tested microorganism.Results: The highest antibacterial activity recorded in Staphylococcus aureus Bacillus cereus and followed by Candida albicans. Antibacterial activity of leaf extracts of A. reticulata was also significant against the tested microorganisms Escherichia coli, Salmonella typhi, Proteus vulgaris, Pseudomonas aeruginosa compared to ciprofloxacin.Conclusion: Based on the above observations, these extracts were further evaluated for their effect on microorganisms causing infections like typhoid fever, urinary tract infections, septicemia, toxic shock syndrome, skin infection, nosocomial infection, arthritis and diarrhoea. The results also suggest that these plants serve a therapeutic purpose in the treatment bacterial infections.


2003 ◽  
Vol 58 (11-12) ◽  
pp. 850-854 ◽  
Author(s):  
Ayşen Özdemir Türk ◽  
Meral Yılmaz ◽  
Merih Kıvanç ◽  
Hayrettin Türk

Abstract In this study, the antimicrobial activity of the acetone, diethyl ether and ethanol extracts of the lichen Cetraria aculeata has been investigated. The extracts were tested against twelve bacteria and eight fungi and found active against Escherichia coli, Staphylococcus aureus, Aeromonas hydrophila, Proteus vulgaris, Streptococcus faecalis, Bacillus cereus, Bacillus subtilis, Pseudomonas aeruginosa, Listeria monocytogenes. No antimicrobial activity against the fungi was detected. It was determined that only one substance in the extracts has antimicrobial activity and it was characterized as protolichesterinic acid. The MICs of the extracts and protolichesterinic acid were also determined.


PHARMACON ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 897
Author(s):  
Falinry Woran ◽  
Defny S. Wewengkang ◽  
Meilani Jayanti

ABSTRACTLissoclinum badium is a type of ascidian that contains bioactive compounds. This study aims to determine of presence of antibacterial activity from extracts and fractions of Lissoclinum badium collected from Mantehage Island Manado against Escherichia coli and Staphylococcus aureus bacteria. Samples were extracted by maceration method using 95% ethanol solvent and fractionated using solvents of chloroform, n-hexane and methanol. Antibacterial activity was carried out by the disk diffusion agar method. The results showed that the ethanol extracts an methanol fraction had activity to inhibit the growth of Escherichia coli bacteria with strong category. Meanwhile, against the Staphylococcus aureus the ethanol extracts, chloroform and n-hexane fractions had ability to inhibit the growth of bacteria with weak category.. Keywords: Antibacterial activity, Lissoclinum badium, Escherichia coli, Staphylococcus aureus.  ABSTRAKLissoclinum badium merupakan salah satu jenis tunikata yang memiliki kandungan senyawa bioaktif yang dapat digunakan untuk pengobatan berbagai penyakit. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antibakteri dari ekstrak dan fraksi  Lissoclinum badium yang diperoleh dari Pulau Mantehage Manado terhadap Escherichia coli dan Staphylococcus aureus. Sampel diekstraksi dengan metode maserasi menggunakan pelarut etanol 95% dan fraksinasi menggunakan pelarut metanol, kloroform, dan n-heksan. Aktivitas antibakteri dilakukan dengan  metode difusi agar cakram kertas. Hasil penelitian menunjukkan bahwa ekstrak etanol dan fraksi metanol memiliki aktivitas untuk menghambat bakteri Escherichia coli dan Staphylococcus aureus dengan daya hambat kuat. Sedangkan untuk fraksi kloroform dan fraksi n-heksan memiliki aktivitas untuk menghambat bakteri Staphylococcus aureus saja dengan daya hambat sedang. Kata kunci: Aktivitas antibakteri, Lissoclinum badium, Escherichia coli, Staphylococcus aureus.


2005 ◽  
Vol 60 (9-10) ◽  
pp. 693-700 ◽  
Author(s):  
Simone M. de Souza ◽  
Franco Delle Monache ◽  
Artur Smânia

Abstract The antibacterial activity of coumarin per se and other 45 coumarin derivatives was tested against strains of Bacillus cereus MIP 96016, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Staphylococcus aureus ATCC 25923. The inhibitory effects of coumarins were affected by their substitution patterns. Osthenol (44) showed the most effective antibacterial activity against Gram-positive bacteria with MIC values ranging between 125 and 62.5 μg/ml. These results suggested that the prenyl chain of 44 at position 8 and the presence of OH at position 7 of the benzenic ring are required for the antibacterial activity against these strains.


2017 ◽  
Vol 6 (2) ◽  
pp. 115
Author(s):  
Desi Sagita ◽  
Muhammad Nurul Ichwani ◽  
Linuria Linuria

Abstract   Background : Sisik naga herbs (Pyyrosiapiloselloides (L) M.G. Price) is an epiphyte plants with compounds of flavonoids and tannins. The compound provides antibacterial potency. This plant is effective for wounds, canker sores, constipation and dysentery. The antibacterial activity of the plant is potentially for development of medicinal plant compounds. The extraction process by  maceration for 3 days with three repetitions. From 1 kg of weight of Sisik naga obtained 4.95% extract. Antibacterial test using the dilution methods. The results showed the Sisik naga herbs provide antibacterial activity with MIC 256 μg/mL and MBC 512 μg/mL against bacteria Pseudomonas aeruginosa and Staphylococcus aureus. As a positive control used a solution of chloramphenicol.(Objective) This research is aimed to knowing the antibacterial activity of ethanol extracts from Sisik naga hersb. Method : The extraction process by  maceration for 3 days with three repetitions with ethanol. Antibacterial avtivity tes by dilution methods. Results :. From 1 kg of Sisik naga obtained 4.95% extract.. The results showed the Sisik naga herbs provide antibacterial activity with MIC 256 μg/mL and MBC 512 μg/mL against bacteria Pseudomonas aeruginosa and Staphylococcus aureus. As a positive control used a solution of chloramphenicol Conclusion : herb Sisik naga extracts showed antibacterial activity to Pseudomonas aeruginosae and Staphylococcus aureus   Key words: antibacterial, sisik naga , dilution methods


2007 ◽  
Vol 2 (9) ◽  
pp. 1934578X0700200 ◽  
Author(s):  
Messaoud Ramdani ◽  
Oualida Rached ◽  
Hocine Laouer ◽  
Meriem El Koli ◽  
Takia Lograda

The essential oils obtained by hydrodistillation from the green branchlets of Cupressus dupreziana A. Camus growing wild in Tassili n'Ajjer (Algeria) were analyzed by GC and GC/MS. The main constituents of the essential oils were α-pinene (27.2% - 44.2%), germacrene D (16.2% - 27.2%) and Δ3-carene (14.2% - 26.7%). The oils showed insignificant activity on the growth of Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853) and Staphylococcus aureus (ATCC 25923), when investigated by the diffusion method.


2002 ◽  
Vol 57 (5-6) ◽  
pp. 483-488 ◽  
Author(s):  
Moacir G. Pizzolatti ◽  
Andreia F. Venson ◽  
Artur Smânia Júnior ◽  
Elza de F. A. Smânia ◽  
Raimundo Braz-Filho

A mixture of flavalignan cinchonains Ia and Ib was isolated from the bark of Trichilia catigua. The structures were established on the basis of spectroscopic data of the natural products and their methylated derivatives including 2D NMR experiments, and compared with data in the literature. These flavalignans exhibited antibacterial activity against Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus.


2021 ◽  
Vol 4 (2) ◽  
pp. e183
Author(s):  
David Mutisya Musyimi ◽  
Tracy Ann Ashioya ◽  
George Opande ◽  
William Omuketi Emitaro

Human pathogenic microorganisms have developed resistance in response to indiscriminative use of commercial drugs. Plants produce many secondary metabolites with microbiocidal activity hence their use in traditional medicine. Herbalists in Kenya use medicinal plants including Solanum incanum in treating microbial infections. Though S. incanum has been used to treat different diseases in humans and animals, there is little information on antimicrobial activities of its extracts against Escherichia coli and Staphylococcus aureus. In this study, phytochemical analysis and antibacterial activity of solanum incanum leaves, roots and seeds extracts were determined. Ethanolic and aqueous extracts of leaf, root and seed of concentrations 25, 50, 75 and 100, and amoxicillin 25 mg/ml (control) with three replications were used for antibacterial analysis by the agar-well diffusion method. The results were subjected to analysis of variance at P < 0.05. Phytochemical screening revealed the presence of alkaloids, flavonoids, glycosides, saponins, steroids and tannins. Solanum incanum exhibited significant antibacterial effect against the two test bacteria. Ethanol extracts were more active than extracts against the bacteria. Ethanol extracts at 100% inhibited growth of Staphylococcus aureus more than the Escherichia coli. The zones of inhibition for Staphylococcus aureus were 35.0±0.6 mm, 30.94±0.3 mm and 30.14±0.64mm for seed, root and leaves respectively.On the other hand, the zones of inhibition for Escherichia coliat 100% ethanol were 27.20±0.06, 23.14±0.12 and 21.0±0.4 seed, root and leaves respectively.The results validate the use of these plants in ethnomedicine and potential of this plant in treating infections caused by the two bacteria.


2014 ◽  
Vol 2 (01) ◽  
pp. 82-85
Author(s):  
R. Kiruthiga ◽  
R. Rakkimuthu ◽  
K. M. Aravinthan

Crotalaria pallida Aiton. Fabaceae has been used for the treatment of various diseases like. The aim of the present study is to assess antibacterial activity of methanolic leaves extract of Crotalaria pallida Aiton. Escherichia coli and Klebsilla pneumonieae showed maximum susceptibility 19±1 and 18.66±0.57 at 25 mg/ml concentration respectively while Pseudomonas aeruginosa, Bacillus sp. and Staphylococcus aureus showed less susceptibility 11±1, 11.33±1.5 and 15±1 at 25 mg/ml concentration respectively. Overall results have proved that C. pallida leaves posses significant antibacterial activity.


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