scholarly journals IMMOBILIZATION OF CELLULASES ON CHITOSAN: APPLICATION FOR SUGARCANE BAGASSE HYDROLYSIS

2021 ◽  
Vol 55 (7-8) ◽  
pp. 829-837
Author(s):  
MARIANA BISINOTTO PEREIRA ◽  
BRUNO LEMOS NOGUEIRA ◽  
INTI DORACI CAVALCANTI MONTANO ◽  
DASCIANA DE SOUSA RODRIGUES ◽  
CARLOS ALBERTO GALEANO SUAREZ
Keyword(s):  
Lignocellulosic Biomass ◽  
Sugarcane Bagasse ◽  
Protein G ◽  
Enzyme Loading ◽  
Glucose Content ◽  
Chitosan Concentration ◽  
Immobilized Cellulase ◽  
Gel Preparation ◽  
Pretreated Sugarcane Bagasse ◽  
Chitosan Gel

In this work, a commercial cellulolytic cocktail was immobilized on glutaraldehyde activated chitosan gel. The chitosan concentration in the gel preparation, pH, immobilization time and enzymatic loading were evaluated. Immobilized cellulases showed better hydrolysis performance when an enzyme loading of 134 mg protein/g carrier was used for immobilization at pH 9.0 for 30 minutes. Hydrolysates with a glucose content of 13.43 and 10.35 g/L were obtained when Avicel and pretreated sugarcane bagasse were used as substrate, respectively. Immobilized cellulase lost 60% of its hydrolysis performance after 8 cycles using Avicel, and 75% after 6 cycles for sugarcane bagasse. The hydrolysis performance associated with the reuse of the immobilized cellulases indicates that an improvement in the immobilization of cellulases, coupled with an improvement in the pretreatment of lignocellulosic biomass, will allow the development of a continuous hydrolysis system with the enzyme retained in the reactor.

scholarly journals On-site integrated production of cellulases and β-glucosidases by Trichoderma reesei Rut C30 using steam-pretreated sugarcane bagasse

2018 ◽  
Author(s):  
Marcella Fernandes de Souza ◽  
Elba Pinto da Silva Bon ◽  
Ayla Sant’ Ana da Silvab
Keyword(s):  
Carbon Source ◽  
Lignocellulosic Biomass ◽  
Trichoderma Reesei ◽  
Sugarcane Bagasse ◽  
Enzyme Preparation ◽  
Integrated Approach ◽  
Aspergillus Awamori ◽  
Pretreated Sugarcane Bagasse ◽  
Hydrolysis Of ◽  
Rut C30

AbstractThe high cost of commercial cellulases still hampers the economic competitiveness of the production of fuels and chemicals from lignocellulosic biomasses. This cost may be decreased by the on-site production of cellulases with the integrated use of the lignocellulosic biomass as carbon source. This integrated approach was evaluated in the present study whereby steam-pretreated sugarcane bagasse (SPSB) was used as carbon source for the production of cellulases by Trichoderma reesei Rut C30 and the produced enzymes were subsequently used for SPSB hydrolysis. An enzyme preparation with a high cellulase activity, of 1.93 FPU/mL, was obtained, and a significant β-glucosidase activity was achieved in buffered media, indicating the importance of pH control during enzyme production. The hydrolysis of SPSB with the laboratory-made mixture resulted in a glucose yield of 80%, which was equivalent to those observed for control experiments using commercial enzymes. Even though the supplementation of this mixture with external β-glucosidase from Aspergillus awamori was found to increase the initial hydrolysis rates, it had no impact on the final hydrolysis yield. It was shown that SPSB is a promising carbon source for the production of cellulases and β-glucosidases by T. reesei Rut C30 and that the enzyme preparation obtained is effective for the hydrolysis of SPSB, supporting the on-site integrated approach to decrease the cost of the enzymatic hydrolysis of lignocellulosic biomass.

Production of cellulases by solid state fermentation using natural and pretreated sugarcane bagasse with different fungi

2019 ◽  
Vol 17 ◽  
pp. 1-6 ◽  
Author(s):  
Gabriella Soares Borges Salomão ◽  
Jacyele Clarindo Agnezi ◽  
Larissa Bastos Paulino ◽  
Luana Borchardt Hencker ◽  
Taísa Shimosakai de Lira ◽  
...  
Keyword(s):  
Solid State ◽  
Sugarcane Bagasse ◽  
Solid State Fermentation ◽  
Pretreated Sugarcane Bagasse

FORMULASI GELHAND SANITIZER DARI KITOSAN DENGAN BASIS NATRIUM KARBOKSIMETILSELULOSA

2017 ◽  
Vol 1 (1) ◽  
pp. 31
Author(s):  
Supomo Supomo ◽  
Yullia Sukawati ◽  
Fredi Basyar
Keyword(s):  
Phase Separation ◽  
Side Effect ◽  
Physical Stability ◽  
Biocompatible Polymers ◽  
Homogeneity Test ◽  
Consistency Test ◽  
Hand Sanitizer ◽  
Gel Preparation ◽  
Dispersive Power ◽  
Chitosan Gel

Chitosan has been widely used in industrial, food, pharmaceuticals and agriculture. Chitosan is a natural biocompatible polymers means that as nature does not have the side effect, non-toxic, can not be easily digested and broken down by microbes (biodegradable). This study aims to determine whether the chitosan may be formulated into dosage gel hand sanitizer that meets the requirements of the physical stability of the gel.Chitosan is formulated with 3 varying concentrations of Na CMC basis of 3%, 4.5% and 6%. Tests conducted gel formulation is the physical stability test which includes organoleptic test, homogeneity, pH test, test dispersive power, viscosity test and test consistency. Testing is done every week for 4 weeks of storage.Results of testing physical properties of chitosan gel hand sanitizer has the shape and color stable but the resulting aroma change during storage. pH gel meet the requirements, the consistency test of phase separation does not occur, the homogeneity test showed no homogeneous gel, gel dispersive power does not meet the requirements, the viscosity of the gel preparation third formula does not meet the requirements of viscosity gel

Addition of Surfactants and Non-Hydrolytic Proteins and Their Influence on Enzymatic Hydrolysis of Pretreated Sugarcane Bagasse

2016 ◽  
Vol 181 (2) ◽  
pp. 593-603 ◽  
Author(s):  
Johanna Méndez Arias ◽  
Anelize de Oliveira Moraes ◽  
Luiz Felipe Amarante Modesto ◽  
Aline Machado de Castro ◽  
Nei Pereira Jr
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Pretreated Sugarcane Bagasse ◽  
Hydrolysis Of
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