Formulation and Evaluation of Antioxidant Peel-Off Face Mask Containing Red Dragon Fruit Rind Extract (Hylocereus polyrhizus Haw.)

Peel-Off face mask is one of the cosmetics that is used to treat skin from free radicals. Peel-off face mask can minimize the effects of free radicals because it contains antioxidant. One of the antioxidant sources is red dragon fruit’s rind (Hylocereuspolyrhizus Haw.). The aim of this study is to obtain Peel-Off face mask of red dragon fruit’s rind extract that contains anthocyanin. Red dragon fruit’s rind was macerated by ethanol 96%, ethyl acetate and n-hexane added citric acid (4:1) solvents. TLC test used Chloroform: ethyl acetate: n-butanol (5:4:1) eluent and sprayed by AlCl3. Antioxidant activity of extract was tested using DPPH (1,1-Diphenyl-2-Picryhidrazyl) method. Peel-Off face mask was made using PVA, HPMC, methyl paraben, propylparaben, propylenglycol, extract, ethanol 96% and aquadest. The rendemen of ethanol 96% extract was 9,476%, ethyl acetate extract was 0,783% and n-hexane extract was 0,631%. The results of TLC test showed yellow spots on the ethyl acetate and ethanol 96% extracts which indicated that extract contained flavonoids (anthocyanin). The results of antioxidant activity test showed that IC50 value of ethanol 96% extract was 189,7422 (AAI=0,2087), ethyl acetate extract was 196,9398 (AAI=0,2011), and n-hexane extract was 385,3664 (AAI=0,1027). The result of the product evaluations showed that all the formulas complied the product requirements such as the organoleptic, homogenity, pH, dispersive power and drying time. Peel-off face mask already meet the product requirements but further research is needed to test the product stability and activity.

STABILITY COMPARISON OF TULASI (Ocimum tenuiflorum L.) LEAF GEL HAND SANITIZER USING 0.5% AND 1% CMC-Na

Background: Hand sanitizers contain 62% alcohol, softener and moisturizer. High alcohol content is able to irritate and make hands dry. A suitable moisturizer is needed for this preparation. Objective: The aim of this study is to evaluate the physical quality of gel hand sanitizer formulation of Ocimum tenuiflorum with CMC-Na as a gelling agent at a concentration of 0.5% and 1% CMC-Na. Methods: Data were collected at week-0,1,2,3,4, and replication is performed three times at each concentration of each measurement. The test results of the spread and adhesion data analysis using SPSS with a 95% confidence level. Results: Dispersive power and adhesion showed no significant difference (p <0.05) at week 0 and week 4 means second-hand sanitizer gel formulation concentration is not stable. Gel hand sanitizer leaves of Tulasi (Ocimum tenuiflorum) at a concentration of 1% CMC-Na stable from week 0 to week 3, while the concentration of 0.5% CMC-Na change at week 2. Gel hand sanitizer leaves of Tulasi (Ocimum tenuiflorum) with a gelling agent CMC-Na should need to be titrated. Conclusion: Gel hand sanitizer leaves of Tulasi (Ocimum tenuiflorum) that use concentrations of 1% CMC-Na as a gelling agent have a physical quality that is more stable than the organoleptic test concentration of 0.5% CMC-Na. Keywords: Hand sanitizer, Ocimum tenuiflorum L., CMC-Na, Physical quality evaluation

Formulasi Sediaan Krim Anti Jerawat Ekstrak Etanol Biji Pepaya Muda (Carica papaya L.) Serta Uji Aktivitasnya Terhadap Bakteri Pseudomonas aeruginosaATCC 27825 dan Staphylococcus aureusATCC 25923 (Anti Acne Cream Formulation for Young Papaya Seed (Carica papaya L.) Ethanol Extract and Activity Test Against Pseudomonas aeruginosa ATCC 27825 and Staphylococcus aureus ATCC 25923)

Background: Young papaya seeds (Carica papaya L.) contained terpenoids, alkaloids karpain and flavonoids that have been examined has antibacterial activity. Purpose: This study aims to obtain anti-acne cream from extract of young papaya seeds (Carica papaya L.) that possess antibacterial activity against Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27825. Methods: Extract of young papaya seeds (Carica papaya L.) was obtained by maceration process using ethanol 96% and the antibacterial activity of extract conducted by well diffusion method. then the extract formulated into anti acne cream dosage and then tested to characteristic properties including pH test, dispersive power test, organoleptic test, homogeneity test, viscosity test and irritation test. Result: The results showed anti acne creams of extract young papaya seeds (Carica papaya L.) produced qualified standard and did not cause irritation. Conclusion: Anti acne cream containing extract concentration of 10% can inhibited bacteria of S. aureus ATCC 25923 with inhibition zone of 17,5 mm and P. aeruginosa ATCC 27825 with inhibition zone of 19,3 mm.Keyword: seeds of papaya (Carica papaya L.), anti acne creamLatar Belakang: Biji pepaya muda (Carica papaya L) mengandung terpenoid, alkaloid karpain dan flavonoid, yang telah diteliti memiliki aktivitas antibakteri. Tujuan: Penelitian ini bertujuan untuk memperoleh sediaan krim anti jerawat dari ekstrak biji pepaya muda (Carica papaya L) yang memiliki aktivitas anti bakteri terhadap bakteri Staphylococcus aureus ATCC 25923 dan Pseudomonas aureginosa ATCC 27825. Metode: Ekstrak biji pepaya muda (Carica papaya L) diperoleh melalui proses maserasi menggunakan pelarut etanol 96%, serta uji aktivitas antibakteri ekstrak dilakukan dengan metode sumuran. Ekstrak selanjutnya diformulasikan menjadi sediaan krim anti jerawat dan dilakukan uji karakterisasi meliputi uji pH, uji daya sebar, uji organoleptik, uji homogenitas, uji viskositas dan uji iritasi. Hasil: Hasil pengamatan menunjukkan sediaan krim anti jerawat ekstrak biji pepaya muda (Carica papaya L) yang dihasilkan memenuhi syarat standar nilai pH, daya sebar dan viskositas. Hasil uji iritasi memperlihatkan formula krim tidak menimbulkan iritasi. Simpulan: Sediaan krim anti jerawat dengan konsentrasi ekstrak 10% dapat menghambat bakteri S.aureus ATCC 25923 dengan hambatan sebesar 17,5 mm dan P. aeruginosa ATCC 27825 dengan hambatan sebesar 19,3 mm.Kata Kunci: biji pepaya muda (Carica papaya L), krim anti jerawat

FORMULATION AND CHARACTERIZATION OINMENT OF ARUM MANIS MANGO (Mangifera indica L.) LEAVES METHANOL EXTRACTS AS AN ANTIBACTERIAL AGAINST Propionibacterium acnes

Background: Acne is caused by several factors including the active secretion of sebaceous sweat glands, hyperkeratosis in the hair infundibulum and the effects of bacteria. One of the plants that has the potential as an antibacterial is the extract of arumanis mango leaves. Method: Determine the Minimum Inhibitory Concentration (MIC) of methanol extract of mango leaves which can inhibit Propionibacterium acnes activity. The antibacterial activity tests were performed using agar diffusion. The ointment formulation, the characteristics of ointment preparations and the ointment activity against P. Acnes are discussed. Result: MIC of methanol extract of mango leaves value is 5 ppm with an inhibition zone of 1 mm. The ointment obtained is white, has distinctive smell, semisolid form, possesses a pH of 4.92 - 5.87, dispersive power of 5.05 - 6.30 cm, adhesive power of 1 - 3.67 seconds, homogeneous and protective. Ointment preparations of methanol extract of mango leaves has activity on P. acnes on the 0 and 15th day of storage. The activities of ointment preparation on day 0 with concentrations of 0, 5, 10 and 15 ppm are 0.00 mm; 10.20 mm; 19.97 mm and 23.60 mm respectively, while the inhibition zones produced by the preparation of ointment on day 15 with concentrations of 0, 5, 10 and 15 ppm are 0.00 mm; 5.71 mm; 9.58 mm and 21.88 mm respectively. Conclusion: Methanol extract of mango leaves (Mangifera indica L.) and oinment preparation are able to inhibit the growth of Propionibacterium acnes.

HOW DO MILITARY CONFLICTS END? LESSONS FOR UKRAINE

The main ways of ending military conflicts, both between states and within the state, are considered. Among the four main ways to end the war are: military victory, negotiated settlement, negotiated truces, peace of sorts is imposed by third parties. Researchers conclude that the best way to promote sustainable peace is a negotiated settlement. The main factor here is the text of the peace agreement, which creates the rules of the game, according to which the key actors in the conflict agree to act. The models of democracy that can be laid down in a peace agreement are analyzed. Among them are consociational democracy, power-dividing, centrifugal and corporate models. The attention is paid to the consociational model, which according to the author, is the best alternative for resolving the armed conflict in the occupied territories of Luhansk and Donetsk regions of Ukraine. The basic idea of power-sharing or consociational democracy is to accommodate the interests of the political elite, which represents each segment in a divided society. The key characteristics of power-sharing are a grand coalition, segment’s autonomy, proportional representation, and mutual veto. All these features can be applied in different categories of power-sharing. These are political, military, economic, and territorial dimensions. Besides, there are three types of power-sharing: inclusive power-sharing, constraining power-sharing, and dispersive power-sharing. The division of different types helps to understand at what stage of conflict resolution, what kind of institutions of power-sharing should be implemented. An analysis of the Minsk Agreement revealed that its text was at odds with current conflict resolution practices, which was one of the reasons why the agreement does not affect conflict resolution.

Optimization of SRC (Semi Refined Carrageenan) and Glucomannan Concentration as Gelling Agent to the Physical Stability Sunscreen Gel of Dry Corncob Extract (Zea mays L.)

BACKGROUND: Corncob is one of crude drug which containing phenolic compounds that can be used as an active ingredient for sunscreen preparations. In this study, extracts of dried corn cob made into a gel formulation using SRC (Semi Refined Carrageenan) and glucomannan as a gelling agent. AIM: This study aims to determine the optimal concentration of SRC (Semi Refined Carrageenan) and glucomannan to the physical stability of the gel. METHODS: Gel made into four formulas with a ratio of 1: 4 and the concentration of each formula was 1%, 1.5%, 2%, 2.5%. Each formula was evaluated for 6 weeks of storage that includes organoleptic test, homogeneity, pH, viscosity, dispersive power, freeze-thaw test and centrifugation. RESULTS: The results showed the fourth formula did not change the organoleptic test and homogeneity test. In the test separation of the freeze-thaw method, the fourth formula was stable, while in the centrifugation test formula 1 and 2 was been separation. pH and viscosity results obtained from statistics with a two-way ANOVA showed significant differences in each formula. CONCLUSION: From the results of this study concluded that the formula 3 with a concentration of 2% was the optimal concentration as a gelling agent.

Formulasi dan Evaluasi Fisik Sediaan Emulgel Tabir Surya Ekstrak Kulit Batang Kayu Manis (Cinnamomum burmannii)

One of the natural ingredients can be used for sunscreen is Cinnamomum burmannii. Sinamaldehid chemical constituents in cinnamon bark extract that has potential as an antioxidant and can be used as a sunscreen content. This study was to determine the levels of cinnamon bark extract ( 1 %, 2 % and 3 % ) which can give physical characteristics, acceptability, which is optimal for preparations with HPMC as a gelling agent, Evaluations include pH, the power spread, the viscosity and acceptability. The results of organoleptic for formula I (1%), II (2%) and III (3%) have a soft texture grainy, the distinctive smell of cinnamon, and all formula has a pale brown color. From the analysis of One-Way ANOVA found significant differences for any dispersive power. But did not have significant differences in pH and viscosity. For acceptability evaluation formula III is the optimal formula to applied softness, easiest formula to flattened and easy to wash.

Surface phenomena related to applications regarding optimum dosages of casein superplasticizer in self-leveling underlayment cements

We experimentally investigated the optimum dosage of casein superplasticizer (SP) in self-leveling underlayments (SLUs). The undersaturated adsorption state of casein in cement pore solution was characterized by zeta potential measurements. Different amounts of casein were dosed in SLU pastes and their dispersion performance was investigated by a mini slump test. Flow values of the SLU pastes were found to increase with casein dosage, but an obvious segregation of the pastes was observed at high dose levels of SP. At an SP dosage of 0.12 wt.% bwob (by weight of binder), a homogeneous SLU paste with a spread flow of >15 cm over a time span of 30 min was obtained, which was identified as the optimum casein dosage for SLUs. Casein was found to adsorb significantly onto the surface of cement, thus producing strong dispersive power at low dose levels.

PENGARUH PEMBUATAN SALEP EKSTRAK DAUN AFRIKA (Vernonia amygdalina) DENGAN BASIS HIDROKARBON DAN ABSORBSI TERHADAP PENYEMBUHAN LUKA BAKAR PADA KELINCI (Oryctolagus cuniculus)

ABSTRACTBurn wound is damaged tissue caused by contact with a heat source which causes infection, so needed some drug for this burn wound. Ointment is a pharmaceutical dosage form used on the skin which is intended for topical effect. In African leaves or Vernonia amygdalina which has many chemical contents and useful effects. This study aimed to test the quality preparation of ointments made from African leaves ethanol extract with hydrocarbon and absorption and its effect on burn wound in rabbits in order to find out effective ointment base for burn wound. The procedures applied were to dry the samples and extract them by maceration. After that, the samples were soaked using ethanol and diaterbath to obtain their thick extract. Later, the base ointment was mixed with thick extract using hot mortar, and tested for its physical properties and effectiveness of burn wound. The results showed that the hydrocarbon and absorbtion ointment base has good quality except for absorption’s poor distribution capacity due to its small dispersive power. African leaves extract ointment with hydrocarbon base and absorption has good quality preparations except for the absorption base’ dispersion power for due to its small dispersive power. To conclude, this study proves that the African leaf extract ointment with hydrocarbon base is very effective for burn wound healing. Key Words: African Leaves, Burn Wound, Ointment. ABSTRAKLuka bakar merupakan rusaknya jaringan yang disebabkan kontak dengan sumber panas yang dapat menimbulkan infeksi, maka dari itu dibutuhkan sediaan yang dapat menangani luka bakar tersebut. Sediaan salep merupakan bentuk sediaan farmasi yang digunakan pada kulit yang dimaksudkan untuk efek topikal. Pada daun Afrika (Vernonia amygdalina) dapat ditemukan banyak kandungan kimia dan efek yang baik juga. Pada penelitian ini bertujuan untuk menguji salep ekstrak etanol daun Afrika dengan basis hidrokarbon dan absorbsi terhadap sediaan mutu dan juga terhadap luka bakar pada kelinci kemudian menemukan basis salep yang efektif untuk luka bakar. Sampel dikeringkan dan diekstraksi secara maserasi dan direndam menggunakan etanol dan diwaterbath untuk mendapatkan ekstrak kental, bahan basis salep dicampurkan dengan ekstrak kental menggunakan mortar panas, diuji sifat fisik dan efektifitas terhadap luka bakar. Basis hidrokarbon sangat efektif untuk luka bakar. Untuk basis hidrokarbon bersifat melunakkan lapisan kulit karena occlusive sehingga akan meningkatkan hidratasi kulit dengan menghambat penguapan air pada lapisan kulit, akibat hidratasi lapisan kulit, mungkin juga akan meningkatkan aktivitas obat. Absorbsi menunjukkan kemampuan basis dalam menyerap air, bukan kemampuan obat diabsorpsi oleh kulit. Salep ekstrak daun Afrika dengan basis hidrokarbon dan absorbsi memiliki sediaan mutu yang baik kecuali pada bagian daya sebar untuk basis absorbsi karena memiliki hasil daya sebar yang kecil, basis hidrokarbon salep ekstrak daun Afrika sangat efektif terhadap penyembuhan luka bakar. Kata Kunci: Daun Afrika, Luka Bakar, Salep.