scholarly journals BIOCONVERSION OF ELLAGITANNINS TO ELLAGIC ACID FROM POMEGRANATE PEELS BY SOLID-STATE FERMENTATION USING ASPERGILLUS NIGER AND RHIZOPUS ORYZAE

2020 ◽  
pp. 1-4
Author(s):  
R. Sundaralingam ◽  
S. Premina ◽  
S. Niren Andrew
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Ellagic Acid ◽  
Fruits And Vegetables ◽  
Rhizopus Oryzae ◽  
Fermentation Broth ◽  
Antioxidant Properties ◽  
Mixed Cultures ◽  
Test Organisms

Ellagic acid an effective polyphenol antioxidant found in many fruits and vegetables. The antioxidant properties of ellagic acid have incited preliminary research into the impending health benefits of ellagic acid consumption. In the present research, an attempt is made to extract ellagic acid from pomegranate peels using solid state cultures of Aspergillus niger, Rhizopus oryzae and mixed cultures of both. The fungus has the potential to convert ellagitannins in pomegranate to ellagic acid as an intermediary metabolite. Test organisms were isolated from soil, identified and screened for production of tannase enzyme. Solid state fermentation of pomegranate husk (10% w/v) using A. niger, R. oryzae and mixed cultures was performed for the production of ellagic acid. Samples were collected daily from the fermentation broth and physico-chemical analyses like changes in the total tannin content and tannase enzyme assay were performed. After fermentation the production of ellagic acid in the medium was determined using HPLC. The amount of ellagic acid produced was 9.1mg (for A.niger), 79.1mg (for R. oryzae), 69.6mg (for mixed culture of both A. niger and R.oryzae).

scholarly journals OPTIMIZATION OF POMEGRANATE PEEL EXTRACTS FOR THE BIOCONVERSION OF THE ELLAGITANNINS TO ELLAGIC ACID USING ASPERGILLUS NIGER, RHIZOPUS ORYZAE AND MIXED CULTURE

2021 ◽  
pp. 59-68
Author(s):  
SUNDARALINGAM R. ◽  
NIREN ANDREW S. ◽  
PREMINA S.
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Mixed Culture ◽  
Ellagic Acid ◽  
Rhizopus Oryzae ◽  
Phenolic Antioxidants ◽  
Pomegranate Peel ◽  
Test Organisms ◽  
Microbiological Techniques ◽  
Pomegranate Peel Extract

Objective: The present study is aimed at optimization of concentration of substrate (pomegranate peel extract) for the production of Ellagic acid using Aspergillus niger and Rhizopus oryzae. Methods: Test organisms were isolated and identified using standard microbiological techniques. Collected pomegranate peels were dried, grained and used as the substrate for solid-state fermentation. Prepared spore suspension of the test organisms was inoculated and incubated at room temperature. At regular intervals of 24 h, samples were drawn and subjected to analysis of reducing sugar, soluble proteins, hydrolysable tannins and production of extracellular tannase using standard methods. Results: The results of the present investigation demonstrated the optimum substrate concentration for the active conversion of ellagitannins to ellagic was 15g of A. niger, 20g for R. oryzae and 15g for mixed culture. Conclusion: From the current work, it was concluded that solid-state bioprocessing of fruit substrates and fruit wastes using fungi has shown to enrich phenolic antioxidants and improve phytochemical consistency.

Ellagic acid production by Aspergillus niger in solid state fermentation of pomegranate residues

2008 ◽  
Vol 35 (6) ◽  
pp. 507-513 ◽  
Author(s):  
Armando Robledo ◽  
Antonio Aguilera-Carbó ◽  
Raúl Rodriguez ◽  
José Luis Martinez ◽  
Yolanda Garza ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Ellagic Acid ◽  
Acid Production

Pectinase production from Aspergillus niger IBT-7 using solid state fermentation

2018 ◽  
Vol 47 (3) ◽  
pp. 473-478 ◽  
Author(s):  
Roheena Abdullah ◽  
Iqra Farooq ◽  
Afshan Kaleem ◽  
Mehwish Iqtedar ◽  
Tehreema Iftikhar
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Fruits And Vegetables ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Local Soil ◽  
Microscopic Studies ◽  
Pectinase Production

Different fungal strains were isolated from the local soil, fruits and vegetables on the basis of pectin hydrolysis. All the isolated strains were identified through microscopic studies and screened for pectinase production using solid state fermentation. The fungal strain identified as Aspergillus niger IBT-7 showed the highest pectinase production. The selected strain was further subjected to optimization through different physical and nutritional parameters to enhance the production of pectinase. Amongst seven different media tested M1 containing rice bran, moistened with Czapek’s nutrient medium showed the highest pectinase production. During optimization maximum pectinase production was achieved after 72 hrs of incubation at 30 ml of moisture content, pH 5.0 and 30°C. Xylose (1.5%) and yeast extract (1%) proved to be best supplemented carbon and nitrogen sources, respectively which gave the highest pectinase production (39.1 U/ml/min).

On-line monitoring of Aspergillus niger GH1 growth in a bioprocess for the production of ellagic acid and ellagitannase by solid-state fermentation

2018 ◽  
Vol 247 ◽  
pp. 412-418 ◽  
Author(s):  
Pedro Aguilar-Zárate ◽  
Jorge E. Wong-Paz ◽  
Luis V. Rodríguez-Duran ◽  
Juan Buenrostro-Figueroa ◽  
Mariela Michel ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Ellagic Acid ◽  
On Line ◽  
Aspergillus Niger Gh1

Exploring the Degradation of Gallotannins Catalyzed by Tannase Produced by Aspergillus niger GH1 for Ellagic Acid Production in Submerged and Solid-State Fermentation

2017 ◽  
Vol 185 (2) ◽  
pp. 476-483 ◽  
Author(s):  
Mónica L. Chávez-González ◽  
Sylvain Guyot ◽  
Raul Rodríguez-Herrera ◽  
Arely Prado-Barragán ◽  
Cristóbal N. Aguilar
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Ellagic Acid ◽  
Acid Production ◽  
Aspergillus Niger Gh1

scholarly journals Food Waste-Assisted Metal Extraction from Printed Circuit Boards: The Aspergillus niger Route

2021 ◽  
Vol 9 (5) ◽  
pp. 895
Author(s):  
Carlotta Alias ◽  
Daniela Bulgari ◽  
Fabjola Bilo ◽  
Laura Borgese ◽  
Alessandra Gianoncelli ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Food Waste ◽  
Solid State Fermentation ◽  
Printed Circuit Boards ◽  
Electronic Waste ◽  
Metal Extraction ◽  
Circuit Boards ◽  
Printed Circuit ◽  
Waste Printed Circuit Boards

A low-energy paradigm was adopted for sustainable, affordable, and effective urban waste valorization. Here a new, eco-designed, solid-state fermentation process is presented to obtain some useful bio-products by recycling of different wastes. Urban food waste and scraps from trimmings were used as a substrate for the production of citric acid (CA) by solid state fermentation of Aspergillus niger NRRL 334, with a yield of 20.50 mg of CA per gram of substrate. The acid solution was used to extract metals from waste printed circuit boards (WPCBs), one of the most common electronic waste. The leaching activity of the biological solution is comparable to a commercial CA one. Sn and Fe were the most leached metals (404.09 and 67.99 mg/L, respectively), followed by Ni and Zn (4.55 and 1.92 mg/L) without any pre-treatments as usually performed. Commercial CA extracted Fe more efficiently than the organic one (123.46 vs. 67.99 mg/L); vice versa, biological organic CA recovered Ni better than commercial CA (4.55 vs. 1.54 mg/L). This is the first approach that allows the extraction of metals from WPCBs through CA produced by A. niger directly grown on waste material without any sugar supplement. This “green” process could be an alternative for the recovery of valuable metals such as Fe, Pb, and Ni from electronic waste.

scholarly journals Biochemical characterisation of a glucoamylase from Aspergillus niger produced by solid-state fermentation

2011 ◽  
Vol 54 (3) ◽  
pp. 559-568 ◽  
Author(s):  
Christiane Trevisan Slivinski ◽  
Alex Vinicius Lopes Machado ◽  
Jorge Iulek ◽  
Ricardo Antônio Ayub ◽  
Mareci Mendes de Almeida
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Biochemical Characterisation

scholarly journals Characterization of Hemicellulolytic Enzymes Produced by Aspergillus niger NRRL 328 under Solid State Fermentation on Soybean Husks

BioResources ◽  
2014 ◽  
Vol 9 (4) ◽  
Author(s):  
Valesca Weingartner Montibeller ◽  
Luciana Porto de Souza Vandenberghe ◽  
Antonella Amore ◽  
Carlos Ricardo Soccol ◽  
Leila Birolo ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation
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