scholarly journals OPTIMIZATION OF POMEGRANATE PEEL EXTRACTS FOR THE BIOCONVERSION OF THE ELLAGITANNINS TO ELLAGIC ACID USING ASPERGILLUS NIGER, RHIZOPUS ORYZAE AND MIXED CULTURE

2021 ◽  
pp. 59-68
Author(s):  
SUNDARALINGAM R. ◽  
NIREN ANDREW S. ◽  
PREMINA S.
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Mixed Culture ◽  
Ellagic Acid ◽  
Rhizopus Oryzae ◽  
Phenolic Antioxidants ◽  
Pomegranate Peel ◽  
Test Organisms ◽  
Microbiological Techniques ◽  
Pomegranate Peel Extract

Objective: The present study is aimed at optimization of concentration of substrate (pomegranate peel extract) for the production of Ellagic acid using Aspergillus niger and Rhizopus oryzae. Methods: Test organisms were isolated and identified using standard microbiological techniques. Collected pomegranate peels were dried, grained and used as the substrate for solid-state fermentation. Prepared spore suspension of the test organisms was inoculated and incubated at room temperature. At regular intervals of 24 h, samples were drawn and subjected to analysis of reducing sugar, soluble proteins, hydrolysable tannins and production of extracellular tannase using standard methods. Results: The results of the present investigation demonstrated the optimum substrate concentration for the active conversion of ellagitannins to ellagic was 15g of A. niger, 20g for R. oryzae and 15g for mixed culture. Conclusion: From the current work, it was concluded that solid-state bioprocessing of fruit substrates and fruit wastes using fungi has shown to enrich phenolic antioxidants and improve phytochemical consistency.

scholarly journals BIOCONVERSION OF ELLAGITANNINS TO ELLAGIC ACID FROM POMEGRANATE PEELS BY SOLID-STATE FERMENTATION USING ASPERGILLUS NIGER AND RHIZOPUS ORYZAE

2020 ◽  
pp. 1-4
Author(s):  
R. Sundaralingam ◽  
S. Premina ◽  
S. Niren Andrew
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Ellagic Acid ◽  
Fruits And Vegetables ◽  
Rhizopus Oryzae ◽  
Fermentation Broth ◽  
Antioxidant Properties ◽  
Mixed Cultures ◽  
Test Organisms

Ellagic acid an effective polyphenol antioxidant found in many fruits and vegetables. The antioxidant properties of ellagic acid have incited preliminary research into the impending health benefits of ellagic acid consumption. In the present research, an attempt is made to extract ellagic acid from pomegranate peels using solid state cultures of Aspergillus niger, Rhizopus oryzae and mixed cultures of both. The fungus has the potential to convert ellagitannins in pomegranate to ellagic acid as an intermediary metabolite. Test organisms were isolated from soil, identified and screened for production of tannase enzyme. Solid state fermentation of pomegranate husk (10% w/v) using A. niger, R. oryzae and mixed cultures was performed for the production of ellagic acid. Samples were collected daily from the fermentation broth and physico-chemical analyses like changes in the total tannin content and tannase enzyme assay were performed. After fermentation the production of ellagic acid in the medium was determined using HPLC. The amount of ellagic acid produced was 9.1mg (for A.niger), 79.1mg (for R. oryzae), 69.6mg (for mixed culture of both A. niger and R.oryzae).

Liberation and recovery of phenolic antioxidants and lipids in chokeberry (Aronia melanocarpa) pomace by solid-state bioprocessing using Aspergillus niger and Rhizopus oligosporus strains

LWT ◽  
2018 ◽  
Vol 87 ◽  
pp. 241-249 ◽  
Author(s):  
Francisc Vasile Dulf ◽  
Dan Cristian Vodnar ◽  
Eva-Henrietta Dulf ◽  
Zoriţa Diaconeasa ◽  
Carmen Socaciu
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Phenolic Antioxidants ◽  
Rhizopus Oligosporus ◽  
Aronia Melanocarpa

scholarly journals Ellagic Acid Recovery by Solid State Fermentation of Pomegranate Wastes by Aspergillus niger and Saccharomyces cerevisiae: A Comparison

Molecules ◽  
2019 ◽  
Vol 24 (20) ◽  
pp. 3689 ◽  
Author(s):  
Federica Moccia ◽  
Adriana C. Flores-Gallegos ◽  
Mónica L. Chávez-González ◽  
Leonardo Sepúlveda ◽  
Stefania Marzorati ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Solid State ◽  
Aspergillus Niger ◽  
Ellagic Acid ◽  
Fold Increase ◽  
Industrial Applications ◽  
Solvent Ratio ◽  
State Culture ◽  
Acid Recovery ◽  
Solid State Culture

Fermentation in solid state culture (SSC) has been the focus of increasing interest because of its potential for industrial applications. In previous studies SSC of pomegranate wastes by Aspergillus niger has been extensively developed and optimized for the recovery of ellagic acid (EA), a high value bioactive. In this study we comparatively investigated the SSC of powdered pomegranate husks by A. niger and Saccharomyces cerevisiae and evaluated the recovery yields of EA by an ultrasound and microwave-assisted 7:3 water/ethanol extraction. Surprisingly enough, the yields obtained by S. cerevisiae fermentation (4% w/w) were found 5-fold higher than those of the A. niger fermented material, with a 10-fold increase with respect to the unfermented material. The EA origin was traced by HPLC analysis that showed a significant decrease in the levels of punicalagin isomers and granatin B and formation of punicalin following fermentation. Other extraction conditions that could warrant a complete solubilization of EA were evaluated. Using a 1:100 solid to solvent ratio and DMSO as the solvent, EA was obtained in 4% yields from S. cerevisiae fermented husks at a high purity degree. Hydrolytic treatment of S. cerevisiae fermented pomegranate husks afforded a material freed of the polysaccharides components that gave recovery yields of EA up to 12% w/w.

Ellagic acid production by Aspergillus niger in solid state fermentation of pomegranate residues

2008 ◽  
Vol 35 (6) ◽  
pp. 507-513 ◽  
Author(s):  
Armando Robledo ◽  
Antonio Aguilera-Carbó ◽  
Raúl Rodriguez ◽  
José Luis Martinez ◽  
Yolanda Garza ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Ellagic Acid ◽  
Acid Production

scholarly journals Anti-Inflammatory Effects of Pomegranate Peel Extract in THP-1 Cells Exposed to Particulate Matter PM10

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Soojin Park ◽  
Jin Kyung Seok ◽  
Jun Yup Kwak ◽  
Hwa-Jin Suh ◽  
Young Mi Kim ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Cell Adhesion ◽  
Particulate Matter ◽  
Adhesion Molecule ◽  
Ellagic Acid ◽  
Interleukin 1 ◽  
Intercellular Adhesion Molecule ◽  
Pomegranate Peel ◽  
Pomegranate Peel Extract ◽  
Peel Extract

Epidemiological and experimental evidence support health risks associated with the exposure to airborne particulate matter with a diameter of <10 μM (PM10). PM10 stimulates the production of reactive oxygen species (ROS) and inflammatory mediators. Thus, we assumed that natural antioxidants might provide health benefits attenuating hazardous effects of PM10. In the present study, we examined the effects of pomegranate peel extract (PPE) on THP-1 monocytic cells exposed to PM10. PM10 induced cytotoxicity and the production of ROS. It also increased the expression and secretion of inflammatory cytokines, such as tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), and monocyte chemoattractant protein-1 (MCP-1), and cell adhesion molecules, such as intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). PPE at 10–100 μg mL−1attenuated the production of ROS and the expression of TNF-α, IL-1β, MCP-1, and ICAM-1, but not VCAM-1, in THP-1 cells stimulated by PM10 (100 μg mL−1). PPE also attenuated the adhesion of PM10-stimulated THP-1 cells to EA.hy926 endothelial cells. PPE constituents, punicalagin and ellagic acid, attenuated PM10-induced monocyte adhesion to endothelial cells, and punicalagin was less cytotoxic compared to ellagic acid. The present study suggests that PPE and punicalagin may be useful in alleviating inflammatory reactions due to particulate matter.

Optimization of ellagic acid accumulation by Aspergillus niger GH1 in solid state culture using pomegranate shell powder as a support

2012 ◽  
Vol 47 (12) ◽  
pp. 2199-2203 ◽  
Author(s):  
L. Sepúlveda ◽  
A. Aguilera-Carbó ◽  
J.A. Ascacio-Valdés ◽  
R. Rodríguez-Herrera ◽  
J.L. Martínez-Hernández ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Ellagic Acid ◽  
State Culture ◽  
Solid State Culture ◽  
Acid Accumulation ◽  
Aspergillus Niger Gh1 ◽  
Shell Powder

On-line monitoring of Aspergillus niger GH1 growth in a bioprocess for the production of ellagic acid and ellagitannase by solid-state fermentation

2018 ◽  
Vol 247 ◽  
pp. 412-418 ◽  
Author(s):  
Pedro Aguilar-Zárate ◽  
Jorge E. Wong-Paz ◽  
Luis V. Rodríguez-Duran ◽  
Juan Buenrostro-Figueroa ◽  
Mariela Michel ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Ellagic Acid ◽  
On Line ◽  
Aspergillus Niger Gh1

Ellagic Acid Production from Biodegradation of Creosote Bush Ellagitannins by Aspergillus niger in Solid State Culture

2008 ◽  
Vol 2 (2) ◽  
pp. 208-212 ◽  
Author(s):  
Antonio Aguilera-Carbo ◽  
Juan S. Hernández ◽  
Christopher Augur ◽  
Lilia A. Prado-Barragan ◽  
Ernesto Favela-Torres ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Ellagic Acid ◽  
Acid Production ◽  
Creosote Bush ◽  
State Culture ◽  
Solid State Culture

scholarly journals Ethanol Extract of Pomegranate (Punica granatum) Peel in Increasing the Expression of Caspase-3 in DSS-Induced Mice

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Kusmardi Kusmardi ◽  
Lyanna Azzahra Baihaqi ◽  
Ari Estuningtyas ◽  
Nurhuda Sahar ◽  
Hadi Sunaryo ◽  
...  
Keyword(s):  
Ellagic Acid ◽  
Caspase 3 ◽  
Ethanol Extract ◽  
Punica Granatum ◽  
Normal Group ◽  
Pomegranate Peel ◽  
Cancer Proliferation ◽  
Pomegranate Peel Extract ◽  
Post Hoc ◽  
Peel Extract

Background. Colorectal cancer (CRC) is a malignancy derived from the glandular epithelial cells in the colon. Patients with inflammatory bowel disease (IBD) are more likely to develop CRC. Cancer proliferation is characterized by the loss of inhibition of apoptosis, which involves caspase-3 activation. This study examined the effects of the pomegranate peel extract on the expression of caspase-3 in mice crypt cells induced by dextran sodium sulfate (DSS) 2%. Methods. The experimental study was done in six groups. All treatments were done in 42 days. The groups were all induced by DSS through water drinking, except for the normal group, which was only given water. The treatments given included the pomegranate extract in two doses (240 mg and 480 mg/kg bw/day), aspirin, and ellagic acid. The specimens were then fixated and stained for the immunohistochemistry scoring for the expression of caspase-3, which was then analyzed statistically. Results. The H-scores of each treatment group were 213.23 ± 8.32 (DSS group), 243.81 ± 18.69 (normal group), 226.10 ± 12.38 (pomegranate peel extract of 240 mg/kg/d), 238.84 ± 15.81 (pomegranate peel extract of 480 mg/kg/d), 227.47 ± 12.15 (aspirin), and 224.01 ± 18.39 (ellagic acid). Statistical differences were found in one-way analysis of variance (ANOVA) and post hoc analysis among the DSS group, normal group, and dose 2 group (pomegranate peel extract of 480 mg/kg/day). Conclusions. The ethanol extract of pomegranate was able to induce apoptosis, which was demonstrated by the increase of caspase-3 expression.

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