scholarly journals Influence of the temperature factor on regeneration features of Silene Jailensis N.I. Rubtzov and Crepis Purpurea (Willd.) M. Bieb. and the content of phenolic substances in vitro

2020 ◽  
pp. 87-96
Author(s):  
I. V. Mitrofanova ◽  
N. N. Ivanova ◽  
A. E. Paliy ◽  
I. N. Paliy ◽  
O. V. Mitrofanova
Keyword(s):  
Phenolic Compounds ◽  
Biochemical Study ◽  
Adventitious Shoots ◽  
Maximum Growth ◽  
Rare Plant ◽  
Temperature Influence ◽  
Phenolic Substances ◽  
Morphogenetic Potential ◽  
High Concentrations

The results of temperature influence on the regeneration of microshoots and the level of phenolic compounds in microshoots of two rare plant species under in vitro conditions are presented. It is shown that the maximum growth of the main and the formation of adventitious shoots, leaves occurred at a temperature of 21- 23°C. Reconnaissance experiments on the biochemical study of organs and tissues of the studied species were conducted. It was found that the leaves contain high concentrations of phenolic substances and are characterized by a wide variety of components. It is shown that as the temperature increased, the content of the sum of phenolic substances increased, which negatively affected the morphogenetic potential of the studied species.

scholarly journals Long-term maintenance of in vitro cultures affects growth and secondary metabolism of St. John's Wort

2010 ◽  
Vol 40 (10) ◽  
pp. 2115-2121 ◽  
Author(s):  
Adriana de Andrade Figueiró ◽  
Cynthia Manira Correa ◽  
Leandro Vieira Astarita ◽  
Eliane Romanato Santarém
Keyword(s):  
Phenolic Compounds ◽  
Secondary Metabolism ◽  
Adventitious Shoots ◽  
In Vitro Shoots ◽  
Culture Production ◽  
Short And Long Term ◽  
Short Term Experiment ◽  
Term Maintenance

Hypericum perforatum is a traditional medicinal plant with wound healing and antidepressant properties. Efficiency of micropropagation is often related to the long term maintenance of tissues in culture, which may alter the secondary metabolism of plants. The objective of this study was to evaluate growth and secondary metabolism of in vitro shoots of H. perforatum on short and long term maintenance of cultures (30 and 100 days). The effect of BA and NAA supplementation was evaluated during 30 days of culture. Adventitious shoots were cultivated on MS medium supplemented with 4.4mM BA alone or in combination with 0.05mM NAA for 30 days. A hormone-free medium was used as control. Shoots cultivated for 100 days were maintained in presence of 4.4mM BA. Biomass, multiplication of shoots, contents of phenolic compounds, flavonoids and hypericin were evaluated. No difference between BA and BA+NAA was observed on growth, multiplication of shoots and levels of flavonoids at the end of 30 days of culture. Production of phenolic compounds was promoted by addition of BA+NAA to the medium, whereas hypericin was increased by the presence of BA. The time of culture (30 and 100 days) affected all the parameters analyzed, except the levels of flavonoids in the short term experiment.

Mineral requirements for the growth of Anabaena spiroides in vitro

1968 ◽  
Vol 46 (5) ◽  
pp. 619-630 ◽  
Author(s):  
Sherry L. Volk ◽  
Harry K. Phinney
Keyword(s):  
Dry Weight ◽  
Maximum Growth ◽  
Major Elements ◽  
Major Nutrient ◽  
Initial Culture ◽  
Upper Klamath Lake ◽  
High Concentrations ◽  
Initial Medium ◽  
Klamath Lake

Anabaena spiroides Klebahn, isolated from Upper Klamath Lake, Klamath County, Oregon, was cultured in Gerloff's modification of Chu's number 10 medium. Preliminary to a study of the major nutrient mineral requirements, the initial culture medium was modified by elimination of sodium silicate, the addition of Tris(hydroxymethyl)aminomethane, and the substitution of hydrogen ferric ethylenediamine di-o-hydroxyphenylacetate (EDDHA) for ferric citrate – citric acid.Provision of mineral nitrogen in the medium was found unnecessary for growth, strongly suggesting that A. spiroides can fix nitrogen, but final proof awaits the development of bacteria-free cultures. Although phosphorus was required in relatively high concentrations to obtain maximum growth, it was apparently accumulated in the cells, allowing them to survive long exposure in phosphorus-deficient media. Several other major elements either were required in very minute amounts or were accumulated by the alga. Iron provided in the alkaline-stable chelate EDDHA was required in lower concentration than in Gerloff's medium. A definite, high requirement for calcium was found.A balanced medium was developed that supported an increase of 100% in dry weight, 23% in chlorophyll content, and 10% in organic nitrogen above that obtained in the initial medium.

scholarly journals Screening of Human CYP1A2 and CYP3A4 Inhibitors from Seaweed In Silico and In Vitro

Marine Drugs ◽  
2020 ◽  
Vol 18 (12) ◽  
pp. 603
Author(s):  
Sung-Kun Yim ◽  
Kian Kim ◽  
SangHo Chun ◽  
TaeHawn Oh ◽  
WooHuk Jung ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
In Silico ◽  
Dependent Manner ◽  
Prosthetic Group ◽  
In Silico Modeling ◽  
Distal Surface ◽  
Ic50 Values ◽  
High Concentrations ◽  
Potential Inhibitors

Phenolic compounds and carotenoids are potential inhibitors of cytochrome P450s. Sixteen known compounds, phenolic compounds and carotenoids from seaweed were examined for potential inhibitory capacity against CYP1A2 and CYP3A4 in silico and in vitro. Morin, quercetin, and fucoxanthin inhibited the enzyme activity of CYP1A2 and CYP3A4 in a dose-dependent manner. The IC50 values of morin, quercetin, and fucoxanthin were 41.8, 22.5, and 30.3 μM for CYP1A2 and 86.6, 16.1, and 24.4 μM for CYP3A4, respectively. Siphonaxanthin and hesperidin did not show any significant effect on CYP1A2, but they slightly inhibited CYP3A4 activity at high concentrations. In silico modeling of CYP’s binding site revealed that the potential inhibitors bound in the cavity located above the distal surface of the heme prosthetic group through the 2a or 2f channel of CYPs. This study presents an approach for quickly predicting CYP inhibitory activity and shows the potential interactions of compounds and CYPs through in silico modeling.

scholarly journals Kinetin and 6-benzyladenine induce different morphogenetic responses in cotyledonary segments of royal poinciana

2019 ◽  
Vol 25 (3) ◽  
pp. 270-275
Author(s):  
Andrey de Oliveira Costa ◽  
Lázara Aline Simões Silva ◽  
Isabella Mendes Duarte ◽  
Vitor Fernandes Sampaio ◽  
Mariana Machado ◽  
...  
Keyword(s):  
Adventitious Shoots ◽  
Woody Species ◽  
Control Treatment ◽  
Mesophyll Cells ◽  
New Information ◽  
Different Types ◽  
High Concentrations ◽  
In Vitro Performance ◽  
Regeneration Systems

Abstract Understanding the in vitro performance of royal poinciana explants cultured in media supplemented with different types and concentrations of cytokinins may aid in the optimization of regeneration systems established for this woody species. In the present study we evaluate the in vitro performance of royal poinciana cotyledonary explants cultured in the presence of high concentrations of 6-benzyladenine (BA) and kinetin (KIN). Cotyledonary segments obtained from in vitro germinated seedlings were inoculated in Murashige and Skoog (MS) medium, supplemented with different concentrations (1, 2, 4, 8 mg L-1) of BA or KIN. In the control treatment, no plant growth regulators (PGRs) were added. After 40 days of culture, regardless of the concentration used, the treatments supplemented with BA presented higher calli percentage and fresh mass compared to treatments supplemented with KIN. Adventitious shoots were mainly observed in BA-treatments. Histological analysis showed that adventitious shoots formed at the periphery of callus formed from mesophyll cells in the regions of the explant sectioning. The results obtained provide new information for the establishment of a micropropagation system for royal poinciana, an important ornamental tree species.

scholarly journals The development of a novel autologous blood glue aiming to improve osseointegration in the bone-implant interface

2020 ◽  
Vol 9 (7) ◽  
pp. 402-411
Author(s):  
Anita Sanghani-Kerai ◽  
Melanie Coathup ◽  
Robyn Brown ◽  
George Lodge ◽  
Liza Osagie-Clouard ◽  
...  
Keyword(s):  
Fibrin Glue ◽  
Platelet Rich Plasma ◽  
Autologous Blood ◽  
Autologous Bone Marrow ◽  
Cost Effective ◽  
Maximum Growth ◽  
Implant Surface ◽  
Bioactive Substances ◽  
High Concentrations

Aims For cementless implants, stability is initially attained by an interference fit into the bone and osteo-integration may be encouraged by coating the implant with bioactive substances. Blood based autologous glue provides an easy, cost-effective way of obtaining high concentrations of growth factors for tissue healing and regeneration with the intention of spraying it onto the implant surface during surgery. The aim of this study was to incorporate nucleated cells from autologous bone marrow (BM) aspirate into gels made from the patient’s own blood, and to investigate the effects of incorporating three different concentrations of platelet rich plasma (PRP) on the proliferation and viability of the cells in the gel. Methods The autologous blood glue (ABG) that constituted 1.25, 2.5, and 5 times concentration PRP were made with and without equal volumes of BM nucleated cells. Proliferation, morphology, and viability of the cells in the glue was measured at days 7 and 14 and compared to cells seeded in fibrin glue. Results Overall, 2.5 times concentration of PRP in ABG was capable of supporting the maximum growth of cells isolated from the BM aspirate and maintain their characteristics. Irrespective of PRP concentration, cells in ABG had statistically significantly higher viability compared to cells in fibrin glue. Conclusion In vitro this novel autologous gel is more capable of supporting the growth of cells in its structure for up to 14 days, compared to commercially available fibrin-based sealants, and this difference was statistically significant. Cite this article: Bone Joint Res 2020;9(7):402–411.

scholarly journals LC-MS/MS Analysis of Phenolic Compounds and In Vitro Antioxidant Potential of Stachys lavandulifolia Vahl. var. brachydon Boiss.

2018 ◽  
Vol 72 ◽  
pp. 28-36
Author(s):  
Muhammed Nuri Bingol ◽  
Ercan Bursal
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Quinic Acid ◽  
Antioxidant Potential ◽  
Antioxidant Power ◽  
Liquid Chromatography Tandem Mass ◽  
Spectrometry Technique ◽  
High Concentrations ◽  
Ethanol Extracts

The identification and quantification of phenolic compounds of Stachys lavandulifolia Vahl. var. brachydon Boiss. by LC-MS/MS (Liquid Chromatography- tandem Mass Spectrometry) technique is the main purpose of the current study. The high concentrations of quinic acid (2534±12 ppb) and chlorogenic acid (1882±92 ppb) were detected by LC-MS/MS.. Another goal of the study is to evaluate the antioxidant activities of both ethanol and aqueous extracts of the plant material. The antioxidant potentials of extracts were determined by using five different in vitro methods including; ABTS (2,2′-Azino-bis-3-ethylbenzothiazoline-6-sulfonic acid), DPPH(1,1-diphenyl-2-picryl-hydrazyl), FRAP (Ferric ions Reducing Antioxidant Power), CUPRAC (Cupric ions Reducing Antioxidant Power), and ferric thiocyanate methods. The results revealed that the aqueous and ethanol extracts of S. lavandulifolia leaves have good antioxidant potential with high phenolic content.

scholarly journals Inhibition of the biosynthesis of sterols by phenyl and phenolic compounds in rat liver

1973 ◽  
Vol 134 (3) ◽  
pp. 737-743 ◽  
Author(s):  
S. Ranganathan ◽  
T. Ramasarma
Keyword(s):  
Phenolic Compounds ◽  
Organic Acids ◽  
Aromatic Ring ◽  
Rat Liver ◽  
Carboxyl Group ◽  
Free System ◽  
A Cell ◽  
High Concentrations

The presence of mitochondria increased the incorporation of [2-14C]mevalonate into sterols in a cell-free system from rat liver. Various phenyl and phenolic compounds inhibited the incorporation of mevalonate when added in vitro. p-Hydroxycinnamate, a metabolite of tyrosine, was the most powerful inhibitor among the compounds tested. Catechol, resorcinol and quinol were inhibitory at high concentrations. Organic acids lacking an aromatic ring were not inhibitory. Two hypocholesterolaemic drugs, Clofibrate (α-p-chlorophenoxyisobutyrate) and Clofenapate [α,4-(p-chlorophenyl)phenoxyisobutyrate], which are known to affect some step before the formation of mevalonate in the biosynthesis of cholesterol in vivo, showed inhibition at a step beyond the formation of mevalonate in vitro. The presence of the aromatic ring and the carboxyl group in a molecule appears to be necessary for the inhibition.

Phenolic compounds from in vitro cultures of Rindera graeca Boiss. & Feldr.

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
K Sykłowska-Baranek ◽  
A Pietrosiuk ◽  
K Graikou ◽  
H Damianakos ◽  
M Jeziorek ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
In Vitro Cultures

In Vitro Effects of Ethanol on Rabbit Platelet Aggregation, Secretion of Granule Contents, and Cyclic AMP Levels in the Presence of Prostacyclin

1989 ◽  
Vol 61 (02) ◽  
pp. 254-258 ◽  
Author(s):  
Margaret L Rand ◽  
Peter L Gross ◽  
Donna M Jakowec ◽  
Marian A Packham ◽  
J Fraser Mustard
Keyword(s):  
Cyclic Amp ◽  
Inhibitory Effect ◽  
Rabbit Platelet ◽  
Inhibitory Effects ◽  
Low Concentrations ◽  
Rabbit Platelets ◽  
High Concentrations ◽  
In Vitro Effects ◽  
Aggregation Of Platelets

SummaryEthanol, at physiologically tolerable concentrations, inhibits platelet responses to low concentrations of collagen or thrombin, but does not inhibit responses of washed rabbit platelets stimulated with high concentrations of ADP, collagen, or thrombin. However, when platelet responses to high concentrations of collagen or thrombin had been partially inhibited by prostacyclin (PGI2), ethanol had additional inhibitory effects on aggregation and secretion. These effects were also observed with aspirin- treated platelets stimulated with thrombin. Ethanol had no further inhibitory effect on aggregation of platelets stimulated with ADP, or the combination of ADP and epinephrine. Thus, the inhibitory effects of ethanol on platelet responses in the presence of PGI2 were very similar to its inhibitory effects in the absence of PGI2, when platelets were stimulated with lower concentrations of collagen or thrombin. Ethanol did not appear to exert its inhibitory effects by increasing cyclic AMP above basal levels and the additional inhibitory effects of ethanol in the presence of PGI2 did not appear to be brought about by further increases in platelet cyclic AMP levels.

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