scholarly journals IDENTIFIKASI JAMUR Aspergillus sp PADA ROTI TAWAR SEBELUM MASA KADALUARSA DI PASAR BURUNGTUNGKU KOTA BANDUNG

2021 ◽  
Vol 9 (2) ◽  
pp. 122-130
Author(s):  
Ni'matul - Murtafi'ah
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Aspergillus Fumigatus ◽  
Aspergillus Flavus ◽  
Cotton Blue ◽  
Aspergillus Sp

Roti tawar adalah salah satu makanan yang berbahan dasar tepung terigu di fermentasikan dengan ragi (Saccharomyces cerevisiae) dan penambahan bahan lainnya. Jamur merupakan mikroorganisme utama berperan penting dalam proses pembuatan dan pembusukan roti. Jamur Aspergillus sp merupakan jamur kontaminan umum pada berbagai substrat salah satunya pada roti tawar.  Tujuan penelitian ini adalah untuk mengidentifikasi jamur Aspergillus sp pada roti tawar sebelum masa kadaluarsa di Pasar Minggu Burung Tungku Kota Bandung. Penelitian dilakukan dengan menggunakan metode deskriptif yaitu dengan isolasi jamur pada media SDA (Sabaroud Dextrose Agar) kemudian disimpan selama 5 hari di inkubator pada suhu 27oC, setelah itu di periksa di bawah mikroskop dengan penambahan larutan LPCB (Lactophenol Cotton Blue). Berdasarkan hasil pengamatan dari 4 jenis roti tawar 3 jenis roti tawar 2 hari sebelum masa kadaluarsa hasilnya positif di temukan jamur kontaminan pada sampel roti tawar 1, 3, dan roti tawar 4. Pada sampel roti tawar 1 dan 3 ditemukan jamur kontaminan Aspergillus fumigatus dan pada sampel roti tawar 4 ditemukan jamur kontaminan Aspergillus flavus. Dari hasil penelitian di atas dapat disimpulkan bahwa roti tawar 2 hari sebelum masa kadaluarsa terkontaminasi oleh jamur golongan Ascomycetes yaitu Aspergillus sp. Kata Kunci: Roti Tawar, Aspergillus sp, Aspergillus flavus, Aspergillus fumigatus.

scholarly journals Isoprenoid phenol and quinone precursors of ubiquinones and dihydroubiquinones [ubiquinones(H2)] in fungi

1971 ◽  
Vol 123 (3) ◽  
pp. 331-339 ◽  
Author(s):  
Ah Law ◽  
D. R. Threlfall ◽  
G. R. Whistance
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Aspergillus Niger ◽  
Aspergillus Fumigatus ◽  
Neurospora Crassa ◽  
Aspergillus Flavus ◽  
Rhodotorula Glutinis ◽  
Alternaria Solani ◽  
Phycomyces Blakesleeanus ◽  
Agaricus Campestris ◽  
Ubiquinone 10

1. Ten moulds and two yeasts were analysed for the presence of 2-polyprenylphenols, 2-polyprenyl(H2)phenols, 6-methoxy-2-polyprenylphenols, 6-methoxy-2-polyprenyl(H2)phenols, 6-methoxy-2-polyprenyl-1,4-benzoquinones, 6-methoxy-2-polyprenyl(H2)-1,4-benzoquinones, 5-demethoxyubiquinones, 5-demethoxyubiquinones(H2), ubiquinones and ubiquinones(H2). 2. The organisms were found to be of three types: (a) those that contained only ubiquinones (Aspergillus fumigatus and Penicillium brevi-compactum) or ubiquinones(H2) (Alternaria solani, Claviceps purpurae and Penicillium stipitatum); (b) those that contained 5-demethoxyubiquinones and ubiquinones (Agaricus campestris, Aspergillus niger, Phycomyces blakesleeanus, Rhodotorula glutinis and Saccharomyces cerevisiae) or 5-demethoxyubiquinones(H2) and ubiquinones(H2) (Aspergillus quadrilineatus and Neurospora crassa); (c) one that contained 2-decaprenyl(H2)phenol, 6-methoxy-2-decaprenyl(H2)phenol, 6-methoxy-2-decaprenyl(X-H2)-1,4-benzoquinone, 5-demethoxyubiquinone-10(X-H2) and ubiquinones(H2) (Aspergillus flavus). 3. Studies were made on the biosynthesis of ubiquinones and ubiquinones(H2) by Asp. flavus, Phyc. blakesleeanus and S. cerevisiae. These provided evidence that in Phyc. blakesleeanus 5-demethoxyubiquinone-9 is a precursor of ubiquinone-9 and that in S. cerevisiae 5-demethoxyubiquinone-6 is a precursor of ubiquinone-6. In addition they yielded results that may be interpreted as providing evidence that in Asp. flavus 6-methoxy-2-decaprenyl(X-H2)-1,4-benzoquinone and 5-demethoxyubiquinone-10(X-H2) are precursors of ubiquinone-10(X-H2).

scholarly journals Isolasi dan Identifikasi Aspergillus Sp. pada Kantung Hawa Puyuh (Cortunix Japonica)

2021 ◽  
Vol 9 (2) ◽  
pp. 134-142
Author(s):  
Mega Cempaka Putri ◽  
Erina Erina ◽  
Mahdi Abrar ◽  
M. Daud AK
Keyword(s):  
Aspergillus Niger ◽  
Aspergillus Fumigatus ◽  
Aspergillus Flavus ◽  
Coturnix Japonica ◽  
Aspergillus Sp

Aspergillosis merupakan penyakit yang disebabkan oleh jamur Aspergillus sp. yang dapat menyerang puyuh muda secara akut dan sebagai penyakit menahun pada puyuh dewasa. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi Aspergillus sp. pada kantung hawa puyuh (Coturnix japonica). Sampel yang digunakan dalam penelitian ini adalah 35 kantung hawa puyuh yang tidak produktif lagi (afkir) diambil secara acak. Isolasi dan identifikasi Aspergillus sp. dilakukan sesuai dengan menggunakan metode Thompson. Sampel dicuci dengan aquadest steril berisi antibiotic gentamicin selanjutnya ditanamkan pada media Sabouraud’s Dextrose Agar (SDA) kemudian diinkubasikan pada suhu kamar selama 3-7 hari. Pertumbuhan koloni Aspergillus sp. diamati morfologinya secara makroskopis dan mikroskopis. Data yang diperoleh dianalisis secara deskriptif. Hasil pemeriksaan menunjukkan bahwa Aspergillus sp. dapat diisolasi dan diidentifikasi pada 24 dari 35 sampel kantung hawa. Berdasarkan hasil penelitian dapat disimpulkan bahwa 68,57% sampel yang diperiksa positif terinfeksi Aspergillus sp. dengan persentase masing-masing spesies Aspergillus fumigatus 40%, Aspergillus flavus 25,7%, dan Aspergillus niger 17,1%.

scholarly journals Seroprevalencia de micosis pulmonares en pacientes con sintomatología de enfermedad respiratoria crónica baja. Alto Huallaga, Perú 2004

2013 ◽  
Vol 67 (2) ◽  
pp. 134
Author(s):  
Walter Gómez ◽  
Miriam Guevara ◽  
Carlos Cortegana ◽  
Pedro Obregon ◽  
Juan Motta ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Histoplasma Capsulatum ◽  
Paracoccidioides Brasiliensis ◽  
Aspergillus Sp ◽  
San Martín

Objetivo: Detectar por serología la presencia de micosis de localización pulmonar (Paracoccidioides brasiliensis, Aspergillus sp. e Histoplasma capsulatum), en pacientes con síntomas de enfermedad respiratoria crónica. Diseño: Estudio transversal. Lugar: Alto Huallaga (selva de los departamentos de Huánuco y San Martín), Perú. Pacientes: Se incluyó personas procedentes de seis distritos (Rupa Rupa, Tocache, Uchiza, Santa Lucia, Aucayacu y Puerto Inca - Sungaro) con diagnóstico de enfermedad respiratoria crónica baja, tres episodios o más de bronquitis al año y/o tos con expectoración por más de tres meses y en quienes se descartó tuberculosis pulmonar mediante baciloscopia directa. Intervenciones: Por cada persona se llenó una ficha clínica-epidemiológica durante abril a septiembre de 2004, seguido de la toma de una muestra de suero (10 mL) para las pruebas de inmunodifusión para Histoplasma capsulatum, Paraccocidiodes brasiliensis, Aspergilus sp, y Aspergillus fumigatus. Principales medidas de resultados: Seroprevalencia de micosis a Histoplasma capsulatum, Aspergilosis spp. y Paracoccidioides brasiliensis. Resultados: De 640 casos, 327 (51,1%) fueron de sexo masculino, edad promedio 37,1 años y tiempo de enfermedad de 7,4 meses. La seroprevalencia de micosis fue 1,7% (11/640): 4 Histoplasma capsulatum y 7 Aspergilosis spp. No se obtuvo casos positivos de Paracoccidioides brasiliensis y, en los casos positivos, solo se encontró asociación con 'baja de peso' (p = 0,004). Conclusión: Si bien la frecuencia micoserológica obtenida fue baja, se sugiere en esta zona endémica considerar a las micosis dentro del diagnóstico diferencial de casos con enfermedades respiratorias crónicas bajas, así como la utilización de técnicas de inmunodifusión para el diagnóstico de estas etiologías.

scholarly journals عزل وتعريف واختبار كفاءة بعض الفطريات في تحلل الهيدروكربون من التربة الملوثة بالنفط

2020 ◽  
Vol 3 (1) ◽  
pp. 78-90
Author(s):  
سعاد محمد خليفة أبوالغيث ◽  
أحلام القمودي محمد زعيط
Keyword(s):  
Aspergillus Niger ◽  
Aspergillus Fumigatus ◽  
Aspergillus Nidulans ◽  
Aspergillus Flavus ◽  
Malt Extract Agar ◽  
Malt Extract ◽  
Extract Agar

استهدفت هذه الدراسة عزل بعض أنواع الفطريات من التربة الملوثة بالهيدروكربون بمصفاة الزاوية لتكرير النفط، حيث تم عزل وتعريف بعض الفطريات مثل Rhizopus, Aspergillus fumigatus, Aspergillus niger, Aspergillus flavus, Aspergillus nidulans وأوضحت نتائج هذه الدراسة أن تواجد وتنوع فطر Aspergillus قد تفوق معنويا مقارنة بتواجد وتنوع فطرRhizopus. كما تم في هذه الدراسة اختبار قدرة وكفاءة الفطريات المعزولة على النمو واستغلال المركبات الهيدروكربونية المتمثلة في زيت الحمادة وزيت الشرارة بتركيز 1% و3%، حيث أوضحت النتائج بأن جنس Rhizopus سجل أعلى معدل للنمو على الوسط الغذائي Malt Extract Agar، وسجل كلا من فطر A. fumigatus وفطر A. flavus معدّل النمو القطري أعلى معنويا من النمو القطري لفطرA. niger  وفطر A. nidulans. هذه المعدّلات العالية تدل على إمكانية استخدام الفطريات المعزولة في المعالجة البيولوجية للتربة الملوّثة بالنفط.

scholarly journals BIBLICAL CHRISTIAN WORLDVIEW ON POST-HARVEST PATHOGENIC FUNGI IN THE TRADITIONAL MARKET CORN SEEDS

2021 ◽  
Vol 5 (1) ◽  
pp. 86
Author(s):  
Grace Purnamasari Christian ◽  
Wahyu Irawati
Keyword(s):  
Aspergillus Niger ◽  
Aspergillus Flavus ◽  
Complex Structure ◽  
Toxic Substance ◽  
Pathogenic Fungi ◽  
Post Harvest ◽  
The Bible ◽  
Aspergillus Sp ◽  
In The Beginning ◽  
And Function

<p>The post-harvest fungi is one of the greatest cause of various diseases. Particularly, in fungus that infects seeds, the toxic substance called aflatoxin is found. This has caused us to wonder: Has fungi been pathogenic fungi since the beginning of creation? The purpose of this research is to study: 1) The Place of Fungi in God’s Original Creation and the Origin of Pathogenic Fungi, 2) Characterization of post-harvest fungus on corn seeds, 3) Aspergillus sp.’s Structure as Proof of God’s Wonderful Original Creation and Providence, and 4) The factors that contribute to the growth of fungi in post-harvest corn seeds and our responsibility. We conclude that in the beginning, fungi were a part of God’s originally good and perfect creation. These fungi were created by God with the structure and function to support its operation in fulfilling God’s creative purpose. The complex structure of Aspergillus niger and Aspergillus flavus that infected the corn seeds showed God’s providence after the Fall. Pathogenic fungi were the result of the Fall of man into sin. Their growth is caused by the failure to give proper care to the crops. But this should not discourage us since the Bible tells us that God is continually working to restore His creation. Therefore, we are called to be stewards of His creation, to develop and preserve whatever is entrusted in our hands, including the crops for God’s glory and under His authority until He has fully renewed and restored everything.</p><p><strong>BAHASA INDONESIA ABSTRACT:</strong> Jamur pasca panen merupakan salah satu penyebab terbesar timbulnya berbagai penyakit, khususnya disebabkan oleh jamur yang menginfeksi biji-bijian dan menghasilkan substansi beracun disebut dengan aflatoksin. Keadaan ini menimbulkan pertanyaan apakah jamur patogenik ada sejak awal penciptaan? Penelitian ini bertujuan untuk mempelajari: 1) Posisi jamur patogenik di awal penciptaan dan asal mula jamur patogenik, 2) karakteristik jamur pasca panen pada biji jagung, 3) struktur <em>Aspergillus</em> sp sebagai bukti dari providensi Allah akan ciptaanNya yang sangat luar biasa, 4) faktor-faktor yang berkontribusi terhadap pertumbuhan jamur pasca panen biji jagung dan Tanggung jawab kita. Dari hasil penelitian ini disimpulkan bahwa pada mulanya, jamur merupakan bagian ciptaan Allah yang sungguh amat baik dan sempurna. Struktur yang kompleks dari <em>Aspergillus niger</em> dan <em>Aspergillus flavus </em>yang menginfeksi biji jagung menunjukkan providensi Allah setelah kejatuhan dosa. Pertumbuhan jamur patogenik merupakan gambaran kejatuhan manusia ke dalam dosa yaitu kegagalan manusia untuk memberikan pemeliharaan yang memadahi pada biji-bijian pasca panen. Namun demikian, keadaan ini tidak mematahkan semangat kita karena Alkitab mengatakan bahwa Allah masih terus bekerja untuk memulihkan ciptaanNya. Oleh karena itu, diharapkan kita semua menjadi pelayan atas ciptaanNya untuk mengembangkan dan menjaga apa yang dipercayakan kepada kita di bawah otoritasNya termasuk biji-bijian untuk memuliakan Allah hingga Dia secara penuh memperbaharui dan memulihkan segala sesuatu</p>

scholarly journals Keanekaragaman Kapang Patogen dan Non Patogen pada Imago, Kokon, dan Larva Instar Keenam Ulat Sutera Liar Attacus atlas (Lepidoptera: Saturniidae)

2019 ◽  
Vol 37 (2) ◽  
pp. 232
Author(s):  
Agustin Indrawati ◽  
Damiana Rita Ekastuti ◽  
Erdina Pangestika ◽  
Reinilda Alwina
Keyword(s):  
Aspergillus Fumigatus ◽  
Fusarium Oxysporum ◽  
Aspergillus Flavus ◽  
Microscopic Observation ◽  
Potato Dextrose Agar ◽  
Aspergillus Versicolor ◽  
Wild Silkworm ◽  
Opportunistic Pathogenic

Attacus atlas is one of several mould species in Indonesia known as kupu-kupu gajah. Information about variety of mould is rarely known. The purpose of this research was to obtain data about variety of pathogenic or non pathogenic mould at imago,cocoon, and sixth larvae phase of wild silkworm A. atlas. Mould was isolated from cocoon, integument, alimentary duct and reproduction duct of imago, trachea, midgut and hindgut, also haemolymph of larvae. Isolated mould was cultured on potato dextrose agar. Isolated mould from cocoon and imago was identified by macroscopic and microscopic observation. The results showed that there were two kind of moulds from cocoon which were Fusarium oxysporum  and Aspergillus flavus. There were four kind of moulds from imago A. atlas which were Aspergillus flavus, Aspergillus versicolor, Aspergillus fumigatus, Fusarium dimerum, and Aspergillus sp.There were three kind of moulds from sixth larvae which were Aspergillus fumigatus, Aspergillus flavus, and Fusarium dimerum. The mould which has opportunistic pathogenic for Attacus atlas were Fusarium oxysporum, Aspergillus  flavus, Aspergillus fumigatus and Fusarium dimerum.

scholarly journals PRESENTATION PATTERN AND FUNGAL AGENTS SPECTRUM CAUSING OTOMMYCOSIS

2018 ◽  
Vol 11 (1) ◽  
pp. 408
Author(s):  
Kassim Dekhil
Keyword(s):  
Candida Albicans ◽  
Aspergillus Niger ◽  
Aspergillus Fumigatus ◽  
Influencing Factors ◽  
Aspergillus Flavus ◽  
Species Distribution ◽  
Alternaria Alternata ◽  
Otitis Externa ◽  
Fungal Species ◽  
The Public

 Objective: This study was aimed to identify the public pattern of presentation, influencing factors, and sort the fungal species, distribution of sex of patients with otomycosis.Results: The predominant complaints were pruritus and found in 76 patients (88.73%), discomfort and pain found in 62 patients (72.09%), aural fullness in 48 patients (55.81%), tinnitus in 34 patients (39.53%), hearing impairment in 50 cases (58.31%), ear discharge in 22 patients (25.58%), and most of the symptoms seen in 36 patients (68.14%). The results showed a total of eight fungal species belong to six different genera, namely, Aspergillus, Candida, Penicillium, Rhizopus, Alternaria, and Cephalosporium were isolated during this study. Among identified fungi, Aspergillus niger was found to be the most prevalent fungal species with 35.71% followed by Candida albicans (27.55%), Aspergillus flavus (10.20%), Aspergillus fumigatus (8.16), Penicillium digitatum (6.12%) and Cephalosporium species (4.08%), and Rhizopus species (5.1%), while Alternaria alternata had the lowest percentage (6.54%).Conclusion: Otomycosis/mycotic otitis externa is still a common problem and there is a rise in the occurrence of otomycosis in latest years, especially in tropical and subtropical humid climates.

Disinfection of selected Aspergillus spp. using ultraviolet germicidal irradiation

2004 ◽  
Vol 50 (3) ◽  
pp. 221-224 ◽  
Author(s):  
Christopher F Green ◽  
Pasquale V Scarpino ◽  
Paul Jensen ◽  
Nancy J Jensen ◽  
Shawn G Gibbs
Keyword(s):  
Aspergillus Fumigatus ◽  
Aspergillus Flavus ◽  
Fungal Spores ◽  
Petri Dish ◽  
Testing Unit ◽  
Ultraviolet Germicidal Irradiation ◽  
Airborne Fungal Spores ◽  
Agar Surface ◽  
Petri Dishes ◽  
Higher Doses

Aims: The efficacy of ultraviolet germicidal irradiation (UVGI) and the UVGI dose necessary to inactivate fungal spores on an agar surface for cultures of Aspergillus flavus and Aspergillus fumigatus were determined. Methods and results: A four-chambered UVGI testing unit with a 9-W, Phillips, low pressure, mercury UVGI lamp in each chamber was used in this study. An aperture was adjusted to provide 50, 100, 150, and 200 µW/cm2 of uniform flux to the surfaces of the Petri dish, resulting in a total UVGI dose to the surface of the Petri dishes ranging from 12 to 96 mJ/cm2. The UVGI dose necessary to inactivate 90% of the A. flavus and A. fumigatus was 35 and 54 mJ/cm2, respectively. Conclusions: UVGI can be used to inactivate culturable fungal spores. Aspergillus flavus was more susceptible than A. fumigatus to UVGI. Significance and impact of the study: These results may not be directly correlated to the effect of UVGI on airborne fungal spores, but they indicate that current technology may not be efficacious as a supplement to ventilation unless it can provide higher doses of UVGI to kill spores traveling through the irradiated zone.Key words: Aspergillus, ultraviolet germicidal irradiation, fungi.

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