scholarly journals Keanekaragaman Kapang Patogen dan Non Patogen pada Imago, Kokon, dan Larva Instar Keenam Ulat Sutera Liar Attacus atlas (Lepidoptera: Saturniidae)

2019 ◽  
Vol 37 (2) ◽  
pp. 232
Author(s):  
Agustin Indrawati ◽  
Damiana Rita Ekastuti ◽  
Erdina Pangestika ◽  
Reinilda Alwina
Keyword(s):  
Aspergillus Fumigatus ◽  
Fusarium Oxysporum ◽  
Aspergillus Flavus ◽  
Microscopic Observation ◽  
Potato Dextrose Agar ◽  
Aspergillus Versicolor ◽  
Wild Silkworm ◽  
Opportunistic Pathogenic

Attacus atlas is one of several mould species in Indonesia known as kupu-kupu gajah. Information about variety of mould is rarely known. The purpose of this research was to obtain data about variety of pathogenic or non pathogenic mould at imago,cocoon, and sixth larvae phase of wild silkworm A. atlas. Mould was isolated from cocoon, integument, alimentary duct and reproduction duct of imago, trachea, midgut and hindgut, also haemolymph of larvae. Isolated mould was cultured on potato dextrose agar. Isolated mould from cocoon and imago was identified by macroscopic and microscopic observation. The results showed that there were two kind of moulds from cocoon which were Fusarium oxysporum  and Aspergillus flavus. There were four kind of moulds from imago A. atlas which were Aspergillus flavus, Aspergillus versicolor, Aspergillus fumigatus, Fusarium dimerum, and Aspergillus sp.There were three kind of moulds from sixth larvae which were Aspergillus fumigatus, Aspergillus flavus, and Fusarium dimerum. The mould which has opportunistic pathogenic for Attacus atlas were Fusarium oxysporum, Aspergillus  flavus, Aspergillus fumigatus and Fusarium dimerum.

scholarly journals IDENTIFIKASI JAMUR PADA LALAT DI YAYASAN TUNAS MULIA BANTAR GEBANG

2019 ◽  
Vol 2 (1) ◽  
pp. 1-6
Author(s):  
Ika Nurfitrianti ◽  
Maulin Inggraini ◽  
Noor Andryan Ilsan
Keyword(s):  
Aspergillus Niger ◽  
Aspergillus Fumigatus ◽  
Fusarium Oxysporum ◽  
Aspergillus Flavus ◽  
Musca Domestica ◽  
Cross Sectional

Pendahuluan: Yayasan Tunas Mulia, Bantar Gerbang merupakan sekolah alam yang terdapat di daerah Bantar Gebang. Siswa Yayasan Tunas Mulia tinggal didaerah TPST Bantar Gebang sehingga banyak ditemukan lalat di sekitar tempat tinggal mereka. Kelompok lalat yang sering kita temui di lingkungan yaitu lalat rumah, lalat hijau dan lalat daging. Lalat dapat menularkan berbagai macam penyakit terutama penyakit yang disebabkan oleh jamur. Tujuan penelitan ini adalah untuk mengidentifikasi jamur pada lalat di Yayasan Tunas Mulia Bantar Gebang. Metode: Metode penelitian ini secara Cross Sectional yaitu mencuplikan seekor sampel lalat dalam satu waktu. Objek penelitian adalah Lalat yang ditangkap di Yayasan Tunas Mulia Bantar Gebang. Hasil: Hasil yang diperoleh dari penelitian ini adalah didapatkan dua jenis spesies lalat yaitu Musca domestica dan Chrysomya megachepala. Jamur yang di dapatkan pada permukaan tubuh lalat Musca domestica yaitu Aspergillus niger, Aspergillus flavus, Fusarium oxysporum, dan pada permukaan tubuh lalat Chrysomya megachepala yaitu Aspergillus fumigatus dan Aspergillus flavus Kesimpulan: Perlu dilakukan penelitian lanjutan untuk mengetahui penyakit yang disebabkan oleh jamur dari penyebaran lalat dilingkungan masyarakat.

QUANTITATIVE STUDIES ON THE SURVIVAL OF FUNGI IN FIVE-YEAR-OLD DRIED SOIL CULTURES

1954 ◽  
Vol 32 (5) ◽  
pp. 673-678 ◽  
Author(s):  
R. G. Atkinson
Keyword(s):  
Aspergillus Fumigatus ◽  
Fusarium Oxysporum ◽  
Population Level ◽  
Potato Dextrose Agar ◽  
Penicillium Janthinellum ◽  
Penicillium Purpurogenum ◽  
Rhizopus Nigricans ◽  
Quantitative Studies ◽  
Plate Counts

Of 51 dried soil cultures of fungi isolated from soil, only 12, comprising seven of the 32 genera represented, were viable after five years. Platings, directly from the cultures, of 5-, 50-, and 500-mgm. samples with oxgall-amended potato dextrose agar revealed extreme variations in viability of surviving fungi. Population levels were so high in cultures of Rhizopus nigricans, Aspergillus fumigatus, A. sydowi, and A. ustus that plate counts were not obtained, even when a 5-mgm. sample was apportioned among five plates. Cultures of Chaetomium indicum and of C. spirale contained an estimated population of approximately one and one half million, and 450,000 viable centers per gram, respectively, the highest counts obtained. The next most viable cultures were those of Fusarium oxysporum var. redolens and of Circinella spinosa, but these contained only about 60,000 and 20,000 centers per gram, respectively. Next came Penicillium janthinellum with an estimated 4000 centers per gram. The least viable cultures were those of Penicillium purpurogenum and of two isolates of F. oxysporum whose population level was down to about 100 to 200 viable centers per gram.

scholarly journals الكشف عن تواجد الفطريات في بعض الأغذية المنتجة محليا والمستوردة )دراسة أولية)

2019 ◽  
Vol 2 (3) ◽  
pp. 71-80
Author(s):  
خولة عبد السلام القمودي ◽  
مروى عبد الوهاب الشرشاري ◽  
مودة الكوني البكوش ◽  
نهى البشير كاكا ◽  
سارة عبد الرحمن هويسه ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Fusarium Oxysporum ◽  
Aspergillus Flavus ◽  
Alternaria Alternata ◽  
Potato Dextrose Agar ◽  
Sabouraud Dextrose Agar

تعد البقوليات بديلاً عن اللحوم لاحتوائها على نسبة عالية من البروتينات، وتدخل في العديد من وصفات الطعام. ويعتبر سوء التخزين من العوامل التي تؤدي إلى زيادة نمو الميكروبات والجراثيم خاصّة الفطريات التي تعمل على إفراز السموم بالأطعمة مما يؤدي إلى إتلافها. الكشف عن تواجد الفطريات في الأغذية المنتجة محلياً والمستوردة وشملت كلا من التوابل كالفلفل وبذور الحمص وثمار التمر المعروضة للبيع بالأسواق. جمعت العينات الغذائية بمنطقه الدراسة لكل من الصنف المحلي والمستورد، في أوعية معقمة لعدد ثلاث عينات من كل نوع تضمنت ( بذور الحمص والفلفل الأحمر والتمر) بمعدل وزني مقداره  500 غرام لكل عينة. ووضعت في كل من بيئة الآجار المغذية وماء الببتون لمدة 6 ساعات ومنها زرعت العينات على أوساط إنمائية وتشخيصية Potato dextrose agar Sabouraud Dextrose Agar لنمو الميكروبات (فطور وخمائر) وحضنت في 025م درجة مئوية لمدة 5 أيام، بعد الحصول على المزارع النقية حضرت العينة الفطرية للفحص المجهري للفطور. أظهرت نتائج العزل وجود خمسة أنواع من الفطريات بالعينات المدروسة حيث تبين أنّ كافة عينات بذور الحمص ملوثة بينما لوحظ أنّ عينات الفلفل الأحمر المستورد ملوثة أكثر من الفلفل المحلي. كما لوحظ عدم تلوث عينة واحدة من أصل عدد ثلاث عينات لثمار التمر. هذا وشملت الفحوص المجهرية للعزل الفطريات التالية: Alternaria alternata،   ,Fusarium  oxysporum Rhizopus spp، Aspergillus flavus و Aspergillus niger . وتشير النتائج إلى أن المواد الغذائية تتعرض للتلوث نتيجة لعدم إتباع الطرق الصحيحة في التخزين والعرض بالأسواق. يجب على مراكز التغّذية الاهتمام بالمواد الغذائية المستوردة بأن تخضع لشروط الصحة العامة بالجودة والقياس لتكون سليمة وصالحة للاستهلاك وكذلك نشر الوعي الثقافي للمستهلكين والموزعين في الحفاظ على الصحة الغذائية والتوصية أيضا باستكمال الدراسة بإجراء مسح شامل لعدد عينات أكثر والتعرف على سمية الفطريات المعزولة.

scholarly journals Characterization of the mbsA Gene Encoding a Putative APSES Transcription Factor in Aspergillus fumigatus

2021 ◽  
Vol 22 (7) ◽  
pp. 3777
Author(s):  
Yong-Ho Choi ◽  
Sang-Cheol Jun ◽  
Min-Woo Lee ◽  
Jae-Hyuk Yu ◽  
Kwang-Soo Shin
Keyword(s):  
Aspergillus Fumigatus ◽  
Pathogenic Fungus ◽  
Hyphal Growth ◽  
Spore Wall ◽  
Mrna Levels ◽  
Chitin Synthesis ◽  
Helix Loop Helix ◽  
Growth Development ◽  
Opportunistic Pathogenic

The APSES family proteins are transcription factors (TFs) with a basic helix-loop-helix domain, known to regulate growth, development, secondary metabolism, and other biological processes in Aspergillus species. In the genome of the human opportunistic pathogenic fungus Aspergillus fumigatus, five genes predicted to encode APSES TFs are present. Here, we report the characterization of one of these genes, called mbsA (Afu7g05620). The deletion (Δ) of mbsA resulted in significantly decreased hyphal growth and asexual sporulation (conidiation), and lowered mRNA levels of the key conidiation genes abaA, brlA, and wetA. Moreover, ΔmbsA resulted in reduced spore germination rates, elevated sensitivity toward Nikkomycin Z, and significantly lowered transcripts levels of genes associated with chitin synthesis. The mbsA deletion also resulted in significantly reduced levels of proteins and transcripts of genes associated with the SakA MAP kinase pathway. Importantly, the cell wall hydrophobicity and architecture of the ΔmbsA asexual spores (conidia) were altered, notably lacking the rodlet layer on the surface of the ΔmbsA conidium. Comparative transcriptomic analyses revealed that the ΔmbsA mutant showed higher mRNA levels of gliotoxin (GT) biosynthetic genes, which was corroborated by elevated levels of GT production in the mutant. While the ΔmbsA mutant produced higher amount of GT, ΔmbsA strains showed reduced virulence in the murine model, likely due to the defective spore integrity. In summary, the putative APSES TF MbsA plays a multiple role in governing growth, development, spore wall architecture, GT production, and virulence, which may be associated with the attenuated SakA signaling pathway.

scholarly journals Discrimination of Aspergillosis, Mucormycosis, Fusariosis, and Scedosporiosis in Formalin-Fixed Paraffin-Embedded Tissue Specimens by Use of Multiple Real-Time Quantitative PCR Assays

2016 ◽  
Vol 54 (11) ◽  
pp. 2798-2803 ◽  
Author(s):  
Elham Salehi ◽  
Mohammad T. Hedayati ◽  
Jan Zoll ◽  
Haleh Rafati ◽  
Maryam Ghasemi ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Aspergillus Flavus ◽  
Ffpe Tissue ◽  
Content Type ◽  
Real Time Quantitative Pcr ◽  
Tissue Sections ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Tissue Specimens ◽  
Formalin Fixed

In a retrospective multicenter study, 102 formalin-fixed paraffin-embedded (FFPE) tissue specimens with histopathology results were tested. Two 4- to 5-μm FFPE tissue sections from each specimen were digested with proteinase K, followed by automated nucleic acid extraction. Multiple real-time quantitative PCR (qPCR) assays targeting the internal transcribed spacer 2 (ITS2) region of ribosomal DNA, using fluorescently labeled primers, was performed to identify clinically important genera and species of Aspergillus , Fusarium , Scedosporium , and the Mucormycetes . The molecular identification was correlated with results from histological examination. One of the main findings of our study was the high sensitivity of the automated DNA extraction method, which was estimated to be 94%. The qPCR procedure that was evaluated identified a range of fungal genera/species, including Aspergillus fumigatus , Aspergillus flavus , Aspergillus terreus , Aspergillus niger , Fusarium oxysporum , Fusarium solani , Scedosporium apiospermum , Rhizopus oryzae , Rhizopus microsporus , Mucor spp., and Syncephalastrum . Fusarium oxysporum and F. solani DNA was amplified from five specimens from patients initially diagnosed by histopathology as having aspergillosis. Aspergillus flavus , S. apiospermum , and Syncephalastrum were detected from histopathological mucormycosis samples. In addition, examination of four samples from patients suspected of having concomitant aspergillosis and mucormycosis infections resulted in the identification of two A. flavus isolates, one Mucor isolate, and only one sample having both R. oryzae and A. flavus . Our results indicate that histopathological features of molds may be easily confused in tissue sections. The qPCR assay used in this study is a reliable tool for the rapid and accurate identification of fungal pathogens to the genus and species levels directly from FFPE tissues.

Study on Inhibitory Activity and Mechanism of Chitosan Oligosaccharides on Aspergillus Flavus and Aspergillus Fumigatus

2021 ◽  
pp. 118673
Author(s):  
Yuan Ke ◽  
Beibei Ding ◽  
Miaomiao Zhang ◽  
Tongjun Dong ◽  
Yang Fu ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Aspergillus Flavus ◽  
Inhibitory Activity ◽  
Chitosan Oligosaccharides

scholarly journals عزل وتعريف واختبار كفاءة بعض الفطريات في تحلل الهيدروكربون من التربة الملوثة بالنفط

2020 ◽  
Vol 3 (1) ◽  
pp. 78-90
Author(s):  
سعاد محمد خليفة أبوالغيث ◽  
أحلام القمودي محمد زعيط
Keyword(s):  
Aspergillus Niger ◽  
Aspergillus Fumigatus ◽  
Aspergillus Nidulans ◽  
Aspergillus Flavus ◽  
Malt Extract Agar ◽  
Malt Extract ◽  
Extract Agar

استهدفت هذه الدراسة عزل بعض أنواع الفطريات من التربة الملوثة بالهيدروكربون بمصفاة الزاوية لتكرير النفط، حيث تم عزل وتعريف بعض الفطريات مثل Rhizopus, Aspergillus fumigatus, Aspergillus niger, Aspergillus flavus, Aspergillus nidulans وأوضحت نتائج هذه الدراسة أن تواجد وتنوع فطر Aspergillus قد تفوق معنويا مقارنة بتواجد وتنوع فطرRhizopus. كما تم في هذه الدراسة اختبار قدرة وكفاءة الفطريات المعزولة على النمو واستغلال المركبات الهيدروكربونية المتمثلة في زيت الحمادة وزيت الشرارة بتركيز 1% و3%، حيث أوضحت النتائج بأن جنس Rhizopus سجل أعلى معدل للنمو على الوسط الغذائي Malt Extract Agar، وسجل كلا من فطر A. fumigatus وفطر A. flavus معدّل النمو القطري أعلى معنويا من النمو القطري لفطرA. niger  وفطر A. nidulans. هذه المعدّلات العالية تدل على إمكانية استخدام الفطريات المعزولة في المعالجة البيولوجية للتربة الملوّثة بالنفط.

scholarly journals PRESENTATION PATTERN AND FUNGAL AGENTS SPECTRUM CAUSING OTOMMYCOSIS

2018 ◽  
Vol 11 (1) ◽  
pp. 408
Author(s):  
Kassim Dekhil
Keyword(s):  
Candida Albicans ◽  
Aspergillus Niger ◽  
Aspergillus Fumigatus ◽  
Influencing Factors ◽  
Aspergillus Flavus ◽  
Species Distribution ◽  
Alternaria Alternata ◽  
Otitis Externa ◽  
Fungal Species ◽  
The Public

 Objective: This study was aimed to identify the public pattern of presentation, influencing factors, and sort the fungal species, distribution of sex of patients with otomycosis.Results: The predominant complaints were pruritus and found in 76 patients (88.73%), discomfort and pain found in 62 patients (72.09%), aural fullness in 48 patients (55.81%), tinnitus in 34 patients (39.53%), hearing impairment in 50 cases (58.31%), ear discharge in 22 patients (25.58%), and most of the symptoms seen in 36 patients (68.14%). The results showed a total of eight fungal species belong to six different genera, namely, Aspergillus, Candida, Penicillium, Rhizopus, Alternaria, and Cephalosporium were isolated during this study. Among identified fungi, Aspergillus niger was found to be the most prevalent fungal species with 35.71% followed by Candida albicans (27.55%), Aspergillus flavus (10.20%), Aspergillus fumigatus (8.16), Penicillium digitatum (6.12%) and Cephalosporium species (4.08%), and Rhizopus species (5.1%), while Alternaria alternata had the lowest percentage (6.54%).Conclusion: Otomycosis/mycotic otitis externa is still a common problem and there is a rise in the occurrence of otomycosis in latest years, especially in tropical and subtropical humid climates.

Disinfection of selected Aspergillus spp. using ultraviolet germicidal irradiation

2004 ◽  
Vol 50 (3) ◽  
pp. 221-224 ◽  
Author(s):  
Christopher F Green ◽  
Pasquale V Scarpino ◽  
Paul Jensen ◽  
Nancy J Jensen ◽  
Shawn G Gibbs
Keyword(s):  
Aspergillus Fumigatus ◽  
Aspergillus Flavus ◽  
Fungal Spores ◽  
Petri Dish ◽  
Testing Unit ◽  
Ultraviolet Germicidal Irradiation ◽  
Airborne Fungal Spores ◽  
Agar Surface ◽  
Petri Dishes ◽  
Higher Doses

Aims: The efficacy of ultraviolet germicidal irradiation (UVGI) and the UVGI dose necessary to inactivate fungal spores on an agar surface for cultures of Aspergillus flavus and Aspergillus fumigatus were determined. Methods and results: A four-chambered UVGI testing unit with a 9-W, Phillips, low pressure, mercury UVGI lamp in each chamber was used in this study. An aperture was adjusted to provide 50, 100, 150, and 200 µW/cm2 of uniform flux to the surfaces of the Petri dish, resulting in a total UVGI dose to the surface of the Petri dishes ranging from 12 to 96 mJ/cm2. The UVGI dose necessary to inactivate 90% of the A. flavus and A. fumigatus was 35 and 54 mJ/cm2, respectively. Conclusions: UVGI can be used to inactivate culturable fungal spores. Aspergillus flavus was more susceptible than A. fumigatus to UVGI. Significance and impact of the study: These results may not be directly correlated to the effect of UVGI on airborne fungal spores, but they indicate that current technology may not be efficacious as a supplement to ventilation unless it can provide higher doses of UVGI to kill spores traveling through the irradiated zone.Key words: Aspergillus, ultraviolet germicidal irradiation, fungi.

scholarly journals In VitroInteractions of Antifungal Agents and Tacrolimus against Aspergillus Biofilms

2015 ◽  
Vol 59 (11) ◽  
pp. 7097-7099 ◽  
Author(s):  
Lujuan Gao ◽  
Yi Sun
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Fumigatus ◽  
Amphotericin B ◽  
Aspergillus Flavus ◽  
Inhibitory Activity ◽  
Aspergillus Terreus ◽  
Antifungal Agents ◽  
Broth Microdilution ◽  
Content Type ◽  
Antagonistic Effects

ABSTRACTAspergillusbiofilms were prepared fromAspergillus fumigatus,Aspergillus flavus, andAspergillus terreusvia a 96-well plate-based method, and the combined antifungal activity of tacrolimus with azoles or amphotericin B againstAspergillusbiofilms was investigated via a broth microdilution checkerboard technique system. Our results suggest that combinations of tacrolimus with voriconazole or amphotericin B have synergistic inhibitory activity againstAspergillusbiofilms. However, combinations of tacrolimus with itraconazole or posaconazole exhibit no synergistic or antagonistic effects.

Sign in / Sign up

Export Citation Format

Share Document