scholarly journals STUDY OF TOLERANCE OF THE DRUG BASED ON QUARTZETIN BY BLOOD INDICATORS IN CLINICALLY HEALTHY DOGS

2021 ◽  
Vol 22 (1) ◽  
pp. 152-159
Author(s):  
N. Lisova ◽  
M. Zhyla ◽  
A. Rozumnyuk ◽  
N. Shkodyak ◽  
O. Pyatnychko ◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Drug Application ◽  
Malonic Dialdehyde ◽  
Biologically Active ◽  
Medicinal Products ◽  
Biochemical Studies ◽  
Flavonoid Quercetin ◽  
Healthy Dogs ◽  
Hematological And Biochemical Parameters ◽  
Reliable Increase

The article presents the results of clinical trials of tolerance of the drug, containing the flavonoid quercetin, on clinically healthy dogs. Quercetin is a biologically active substance that can have antioxidant, membrane, gastro-, nephro-, hepato-, cardioprotective, antiplatelet, anti-inflammatory effects. The flavonoid quercetin belongs to the aglycones of many plant flavonoid glycosides of higher plants. Some pharmacological effects of quercetin have been discovered recently, but the prospects of its use in veterinary medicine are being intensively studied, given its promising positive effects on the animals` body. The bioavailability of quercetin is highest in combination with pectin and insoluble oligosaccharides, which is associated with changes in the quantitative and qualitative composition of the mammals’ intestinal micro flora. A prerequisite for the development and testing of new veterinary medicinal products in accordance with the requirements of the international community for the registration of medicinal products is the conduct of clinical trials in compliance with the requirements of "Good Clinical Practice" (GCP). The introduction of biologically active drugs in the practice of veterinary medicine requires careful researches on target species of animals. Therefore, to assess the tolerability, safety and efficiency of the drug containing quercetin, a comprehensive clinical trial was performed on small pets (dogs). The study was conducted in veterinary clinics on healthy dogs of all ages, genders and breeds. The drug was used at a dose of 4 mg/kg body weight orally with food for 30 days. Blood sampling in test animals was performed before drug use and on days 30 and 60 of the experiment. The effect of the drug on the body was evaluated by clinical indicators, hematological and biochemical parameters of dogs' blood. Stabilized EDTA blood was used for morphological studies and blood serum for biochemical studies. According to the evaluation of the animals’ clinical condition and the obtained data of laboratory studies, it was found that the tolerance of the studied drug, administered orally to healthy dogs, was good. According to the results of comparison of clinical, hematological and biochemical parameters on the 30th and 60th day of the study with the initial data obtained before the use of the study drug, no negative changes were detected. Hematological studies indicated the activation of hematopoietic processes, normalization of leukogram parameters on 30th day after the drug application. Changes in the lymphocytes` content in the leukogram and the γ-globulin fraction in the protein-gram indicated a strengthening of dogs` immune protection. The results of biochemical studies of dogs's blood on the 30th day showed a reliable increase, within normal limits, in glucose, creatinine contents, increased catalase and alanine aminotransferase activity and decreased gamma-glutamyltransferase activity and the contents of diene conjugates and malonic dialdehyde. Activation of protein metabolism was established, which was indicated by a reliable increase in the total serum protein content and albumin content in the dogs` proteinogram. On the 60th day of the drug application no significant changes in hematological and biochemical parameters of the blood of dogs were detected. There was a slight decrease in the activity of catalase and SOD and a decrease in the content of malonic dialdehyde in the serum of dogs, compared with the values on the 30th day. This, in turn, showed a decrease in the intensity of the formation of toxic compounds in animals and pronounced antioxidant properties of the drug Hepanephran.

scholarly journals STUDY OF TOLERANCE OF DRUG BASED ON QUARTZETIN ON CLINICALLY HEALTHY CATS

2020 ◽  
Vol 21 (2) ◽  
pp. 96-104
Author(s):  
N. E. Lisova ◽  
M. I. Zhyla ◽  
N. V. Shkodyak ◽  
A. V. Rozumniuk ◽  
O. P. Laptiy
Keyword(s):  
Clinical Trials ◽  
Catalase Activity ◽  
Biochemical Parameters ◽  
Physiological State ◽  
Malonic Dialdehyde ◽  
Biologically Active ◽  
Medicinal Products ◽  
Diene Conjugates ◽  
Morphological Studies ◽  
Hematological And Biochemical Parameters

The article presents the results of clinical trials of tolerability of the drug, containing the flavonoid quercetin, on clinically healthy cats. Quercetin is a biologically active substance found in many plants. Some pharmacological effects of quercetin have been discovered recently and are being intensively studied. The results of numerous studies show that quercetin may possess antioxidant, membraneprotective, gastro-, nephro-, hepato-, cardio-protective, antiplatelet, anti-inflammatory action. The bioavailability of quercetin is highest in combination with pectin and insoluble oligosaccharides, which is associated with changes in the quantitative and qualitative composition of the intestinal microflora. One of the prerequisites for the development and testing of new veterinary medicinal products, both in the context of national legislation and the requirements of the international community for the registration of medicinal products, is to conduct clinical trials in accordance with Good Clinical Practice (GCP). This is especially true of drugs with a new substance, the pharmacological properties and effectiveness of which have not been sufficiently studied. Therefore, to assess the tolerability, safety and efficacy of the drug containing quercetin, a comprehensive clinical trial was performed on small pets (cats). The study was conducted in veterinary clinics on healthy cats of all ages, genders and breeds. The drug was administered orally at a dose of 4 mg/kg body weight with food for 30 days. Blood sampling was performed before drug use and on 30 and 60 days of the experiment. The effect of the drug was assessed by clinical indicators, as well as hematological and biochemical parameters of the blood of cats. Stabilized EDTA blood was used for morphological studies and blood serum for biochemical studies. According to the assessment of the animal clinical condition and laboratory data, the tolerability of the studied drug when administered orally to clinically healthy cats was good. According to the results of comparison of clinical, hematological and biochemical parameters obtained on the 30th and 60th day of the experiment, and before the use of the drug, no negative changes were detected. The results of biochemical tests of cats serum blood on 30th day indicated a reliable increase, within normal limits, in glucose, urea, creatinine content, increased alanine aminotransferase and catalase activity, decreased gamma-glutamyltransferase activity and decreased content of diene conjugates and malonic dialdehyde – main peroxide oxidation indexes. Serum protein spectrum parameters were stable throughout the experiment. On the 60th day of drug application, recovery to the initial values of majority cats serum parameters was detected, compared with the values obtained on the 30th day of the experiment. According to the indicators of the antioxidant system of cats, the activity of serum superoxide dismutase and the content of diene conjugates during this period of the experiment remained at the level of the 30th day. Serum catalase activity decreased, compared to the values on the 30th day, but did not differ significantly from initial indices, and the content of malonic dialdehyde in the serum of animals decreased reliably. This indicated a decrease in the intensity of the formation of toxic compounds in the cats body and pronounced antioxidant properties of the drug “Hepanephran”. The results of the studies show that when using the drug for 30 days there was an increase in body tone, activation of hematopoiesis and antioxidant protection, stability of protein metabolism, serum biochemical profile and improvement of the physiological state of the study animals as a whole.

Influence of increased doses of fenbendazole supramolecular complex on sheep’s organism

2018 ◽  
Vol 12 (2) ◽  
pp. 46-52 ◽  
Author(s):  
A.I. Varlamova
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Biochemical Parameters ◽  
Negative Influence ◽  
Supramolecular Complex ◽  
Control Group ◽  
Blood Cell Count ◽  
Russian Scientific ◽  
Russian Scientific Research Institute ◽  
Hematological And Biochemical Parameters

The purpose of the research: study of the influence of increased doses of fenbendazole supramolecular complex (FSMC) on sheep’s organism. Materials and methods. The experiment was carried out at the Podolsk Department of All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K. I. Skryabin on 20 manorial invasion-free sheep aged 2-3 years old. Animals were divided according to the principle of analogues into 4 groups, 5 heads in each group. Animals of the 1, 2 and 3 group were orally administered with FSMC given as a single dose of 2, 6, 10 mg/kg, respectively, according to the active substance, i.e in therapeutic and in a dose increased by 3 and 5 times. Sheep of the fourth group didn’t receive the drug and they were as control. Study of clinical, hematological and biochemical parameters of animals from all groups was conducted 1 day before and in 1, 3, 5 days after administration of the drug by means of standard methods. Results and discussion. FSMC in therapeutic dose as well as in a dose increased by 3 and 5 times doesn’t have negative influence on clinical, hematological and biochemical parameters of the sheep. State of the sheep, which received the drug in doses of 20, 60, 100 mg/kg, was within the physiologically normal state and didn’t differ from the state before administration of the drug and from the animals of the control group. Drug security index exceeds 5. Red blood cell count, white blood cell count, hemoglobin count, leukogram parameters as well as biochemical parameters of blood: activity of alkaline phosphatase and amylase, bilirubin, creatinine, urea and glucose counts were within normal limits and didn’t differ from the parameters of the control animals.

The influence of probiotic drug Biolax-U on morphological and biochemical parameters of blood in cows

2020 ◽  
pp. 70-76
Author(s):  
A. Farkhutdinova
Keyword(s):  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Experimental Studies ◽  
Positive Influence ◽  
Biologically Active ◽  
Feed Consumption ◽  
Control Group ◽  
Animal Blood ◽  
Black And White ◽  
White Breed

The expediency of using the probiotic drug Biolax-U in the rations of lactating and dry cows of Black-and-White breed has been studied and scientifically proved. The influence of this drug on the morphological and biochemical parameters of animal blood has been determined. The composition of the probiotic drug Biolax-U consists of eight strains of a balanced complex of living lactic acid bacteria, yeast and actinomycetes, as well as biologically active substances. For experimental studies conducted on 2 groups of cows have been selected by the method of pairs-analogs, the influence of feeding of feed have been prepared with the use of the microbiological drug with probiotic action Biolax-U on feed consumption, morphological and biochemical changes in blood has been studied. Analysis of the rations of cows indicates that the content of calcium, phosphorus, magnesium, sulfur and trace elements such as manganese, copper, zinc and cobalt in the feed has been reduced. It has been found by studies that the use of probiotic drug Biolax-U had the positive influence on the feed intake, digestibility and better assimilation of feed nutrients and contributed to the improvement of blood morphological and biochemical parameters. For example, in animals of the experimental group hemoglobin has increased by 3,6 % (Р < 0,05), red blood cells by 10,1 (P ≤ 0,05), the content of leukocytes by 4,43, carotene by 9,6, glucose by 3,4; reserve alkalinity by 15,6, calcium by 19,4 (P ≤ 0,01), and phosphorus by 18,4 % compared with the control group of animals. During the lactation significantly improved hematological parameters including alkalinity reserve by 5,11 %, calcium level by 11,1, phosphorus by 8,6, hemoglobin by 14,2 %, the content of erythrocytes by 12,3, leukocyte by 3,86, and carotene 11,1 % against the parameters of animals of control group. The improvement of morphological and biochemical parameters of blood of cows indicates about stimulating effect on hematopoiesis and the general clinical condition of animals that contributes to the improvement of metabolic processes when using feed prepared with the use of microbiological drug with probiotic action Biolax-U.

scholarly journals Exploring alterations in hematological and biochemical parameters, enzyme activities and serum cortisol in Besnoitia besnoiti naturally infected dairy cattle

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Luca Villa ◽  
Alessia Libera Gazzonis ◽  
Sergio Aurelio Zanzani ◽  
Silvia Mazzola ◽  
Alessia Giordano ◽  
...  
Keyword(s):  
Enzyme Activities ◽  
Biochemical Parameters ◽  
Statistical Significance ◽  
Clinical Signs ◽  
Serum Cortisol ◽  
Lower Percentage ◽  
Besnoitia Besnoiti ◽  
Laboratory Parameters ◽  
Bovine Besnoitiosis ◽  
Hematological And Biochemical Parameters

Abstract Background Besnoitia besnoiti is an Apicomplexan protozoa causative of bovine besnoitiosis, a chronic and debilitating disease of cattle, with a variety of pathological findings that could alter some laboratory parameters. A study was conducted in a bovine besnoitiosis endemically infected dairy herd located in Italy characterized by high intra-herd seroprevalence and cattle with clinical signs of the disease. In the study, alterations in laboratory parameters, i.e. hematological and biochemical parameters, enzyme activities and serum cortisol levels, in Besnoitia besnoiti naturally infected cows were investigated in depth. Methods Laboratory parameters in 107 cows, of which 61 were seronegative and 46 were seropositive to B. besnoiti, including 27 with clinical signs of bovine besnoitiosis, were compared. Generalized linear models were used to evaluate the effect of Besnoitia infection on the considered laboratory parameters. Results Hematological analyses revealed that B. besnoiti infection determined a significant alteration to the leukocyte differential, with a higher percentage of granulocytes and a lower percentage of lymphocytes in seropositive and clinically affected animals (Mann–Whitney U-test, P = 0.022); erythrocyte and platelet counts did not show any difference between the considered groups of cows. Biochemistry tests evidenced that the parasite infection influenced serum protein values in seropositive cows and glutamate dehydrogenase values in clinically affected animals. No or only slight differences were revealed for all of the other biochemical and enzyme activity parameters in B. besnoiti-infected animals. In addition, despite the lack of statistical significance, seropositive and clinically affected cows evidenced higher concentrations of serum cortisol values compared to seronegative animals. Conclusions Although physiological, pathological and farm-related factors could have influenced the results in investigated animals, further studies involving more animals from different farms would be advisable to infer the role of B. besnoiti on these alterations, since laboratory parameters could help veterinarians in the diagnosis of bovine besnoitiosis in cattle.

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