Results determination of need patients to implantology dentistry help in depending of extent’s occlusion defects in different territorial-administrative zone of Tajikistan

Aim. To define the needs of patient in implantology dentistry help in depending of extent defect teeth rows beside populations living in different region of the Republic of Tajikistan.Material and methods. In the article are presents the results determination of needs patients to implantology dentistry help in depending of extent defect teeth rows beside populations living in different region of the Republic of Tajikistan. In examined group entered dentistry patients both sexual difference with defect of the teeth rows which were divided into 3 groups: 1st group formed the 372 patients with small defect of the teeth rows (absence 1–3 teeth); in 2nd group entered 783 examined patients with average extent (absence 4–6 teeth) of the defect of teeth row; 3rd group formed 109 patients with greater defect of the teeth rows (absence more than 6 teeth). For the reason studies of extent defect teeth rows organized clinical and epidemiological examination of the orthopedic status in different territorial-administrative zone.Results. Amongst examined persons in respect of defect of the teeth rows by small extent exists reliable reduction their prevalence on measure of the increase the age, then comparatively occlusion defects with average and big extent noted reliable increase of the factor of prevalence.Conclusions. Got material allow to improve rendering of implant’s prosthesis in depending of extent occlusion defects and hereinafter completely shorten the risk of invalidation chewing device.

Evaluation of the Effectiveness of the Fungicide Comissar Against Rice Pyricularia

The results of research on the effectiveness of the fungicide Comissar are presented, EC (active agents propiconazole, 300 g/l and tebuconazole, 200 g/l) against rice Pyricularia, in the conditions of laboratory and vegetation experiments. The experiment was conducted in 2016-2017 in the Primorsky Territory. The fungicide was used at the consumption rate of 0.3 l/ha and 0.4 l/ha one time. In the primary laboratory tests on pure culture Pyricularia oryzae a high inhibitory activity of the drug against the coastal population of rice Pyricularia was revealed. It is noticed that Comissar significantly inhibits the growth of fungal colonies in all tested concentrations. Treatment of the nutriculture medium with solutions of the drug provides a significant preventive effect on the development of P. oryzae compared to an untreated control. The effectiveness of the drug was 100% on the 7th day and 89.3-90.4% on the 14th day. It was found that the use of the drug as a fungicide for the treatment of vegetative rice plants significantly reduces the development of Pyricularia. Comissar has a high biological efficacy against the pathogen at 47.4% (0.3 l/ha) and 62.7% (0.4 l/ha). The use of the fungicide contributed to the active growth of plants and it increased the main productivity indicators: the length of the panicle by 2.08-32.17 cm, the number of filled grains by 3.0-4.5 pcs. and their mass by 0.21-0.24 g per plant, as well as the mass of 1000 grains by 4.91-5.51 g. The maximum reliable increase in grain yield by 3.77 g/vessel was obtained in the variant using a fungicide at the minimum consumption rate.

Experience of long-term use of denosumab in women with osteoporosis and various concomitant diseases

Background: Possible differences in the results of planned RCTs and real clinical practice were the reason for the analysis of long-term therapy with denosumab in patients with osteoporosis (OP) of various origins on an outpatient basis.Aim: To assess the effectiveness of long-term administration of denosumab in terms of the effect on BMD and markers of bone metabolism, tolerance and consequences of drug withdrawal in patients with OP of various etiologies.Materials And Methods: A retrospective analysis of the outpatient records of women with OP of various etiology, who were observed at the FSBI «NMRC TPM» from 1 to 10 years and regularly received denosumab 60 mg once every 6 months subcutaneously (at least 2 injections), was carried out. All completed examination and anthropometric research; DXA of the lumbar spine and proximal femur (PF); laboratory tests: marker of bone resorption CTx (β-crosslaps) in blood serum; survey on the presence of adverse events.Results: The study included 148 patients who were divided into 2 groups: 1 (N=98) - did not take anti-osteoporotic therapy (AT), 2 (N=50) - who took AT before the appointment of denosumab. Long-term therapy with denosumab was associated with a steady and reliable increase in BMD in the spine and PF, as well as a decrease in the concentration of CTx of both those who didn’t take and who previously took AT. In 54% of patients BMD in the spine reached values of osteopenia, in 43.4% of women target BMD values in the femoral neck were determined. During the first year of therapy, there was a decrease in the concentration of CTx by 67% in those who didn’t take AT and by 58% in those who had previously taken AT. Discontinuation of denosumab therapy without subsequent administration of AT was associated with a significant decrease in BMD in the spine (by 4.4-8.2%) during the first year after discontinuation of the drug.Conclusion: Denosumab therapy effectively increases BMD in the spine and PF and decreases CTx levels both in untreated patients and in those who previously received AT. It is necessary to discontinue therapy, further management of the patient should be discussed to prevent «withdrawal syndrome».

Dose-dependent effect of chronic exposure to lead acetate on the dynamics of the content of delta-aminolevulinic acid and essential trace elements in the serum of laying hens

One of the most global and dangerous pollutants is considered to be lead, due to its ability to accumulate in living organisms, to be included in the metabolic cycle, to form highly toxic organometallic compounds, not being biodegradable. To study the chronic effects of lead acetate, an experiment was performed on laying hens of Lohmann Brown cross. For research, one control and three experimental groups were formed, 20 chickens in each. Birds of the experimental groups were administered lead acetate daily with compound feed in doses (in terms of metal): Group I – 50 mg/kg of feed, II – 75 mg/kg of feed, III – 150 mg/kg of feed. Access to water was not restricted. The birds of the control group received a complete diet without lead acetate. On the 30th, 90th, 120th days of the experiment and the 20th day after withdrawal of the toxicant (the 140th day of the experiment), we took blood samples from birds for biochemical and toxicological studies. The concentration of delta-aminolevulinic acid (δ-ALA) in the serum of laying hens was determined by reversed-phase high-performance liquid chromatography. Determination of the content of lead in the serum of laying hens was performed by X-ray fluorescence analysis. The concentration of total calcium, inorganic phosphorus, and magnesium in the serum of the laying hens was determined using production kits. We detected the dose-dependent chronic effect of lead acetate on the dynamics of the content of delta-aminolevulinic acid and essential trace elements in the serum of laying hens. The administration of lead acetate into the body of productive poultry for 120 days in doses of 50, 75, and 150 mg/kg with feed did not cause visible clinical signs of poisoning, but caused a violation of porphyrin metabolism, manifested in a reliable increase in the concentration of delta-aminolevulinic acid in serum on the 30th, 90th and 120th days of the experiment. It has been experimentally proven that the administration into the body of laying hens of lead acetate in doses of 50, 75 and 150 mg/kg of feed for 120 days leads to the maximum accumulation of lead in the serum on the 30th, 90th, and 120th days of the experiment. Prolonged administration of lead acetate to laying hens at high concentrations is characterized by a reliable decrease in the content of total calcium, inorganic phosphorus and magnesium in the serum of laying hens and a reliable increase in the content of ferrum. In the future it is necessary to study the intensity of lipid peroxidation processes in laying hens under chronic exposure to lead acetate.

Application of chelated fertilizers on irrigated vegetable crops in the Saratov Zavolzhye

The effect of chelated fertilizers that produced by the NPO "Power of Life" on the productivity of the main vegetable crops was studied in field experiments on irrigated terraced dark chestnut soils of the Saratov Zavolzhye (Trans-Volga region). It was established that the yield of the vegetable crops under study increases with the use of fertilizers based on humic acids. The increase in yield due to reasil micro hydro mix was: 35.8% of cucumber, 28.3% of red beet roots, 22.6% of tomato, 20.9% of late white cabbage, 9.1% of onion, 6.8% of carrot roots. The reasil micro hydro mix was more effective than potassium-sodium humate with trace elements, for all the studied vegetable crops under study. The average increase in productivity was 20.6% versus 16.0%. The effect of chelated microfertilizers on the yield of vegetable crops was ambiguous. A significant reliable increase in yield due to all the studied chelated microfertilizers, used both together with reasil micro hydro mix, and together with potassium-sodium humate with trace elements, was established only when growing onions. An additional increase in the yield of red beet roots 4.42 t / ha was obtained only when treated with reasil humic N on the background of potassium-sodium humate with trace elements. An additional increase in the yield of late white cabbage was 9.23 and 8.15 t / ha when treated with reasil Mg, respectively, against the background of reasil micro hydro mix and potassium-sodium humate with trace elements. On tomatoes, chelated microfertilizers were effective only against the background of potassium-sodium humate with trace elements. Cucumber responded positively to all chelated microfertilizers on the background of potassium-sodium humate with trace elements, as well as to reasil humic N and reasil Ca/Mg/B on the background of reasil micro hydro mix. On carrots, a significant increase in yield was obtained when treated with reasil Ca, reasil Ca/ Mg/ B and reasil humic N against the background of reasil micro hydro mix.

STUDY OF TOLERANCE OF THE DRUG BASED ON QUARTZETIN BY BLOOD INDICATORS IN CLINICALLY HEALTHY DOGS

The article presents the results of clinical trials of tolerance of the drug, containing the flavonoid quercetin, on clinically healthy dogs. Quercetin is a biologically active substance that can have antioxidant, membrane, gastro-, nephro-, hepato-, cardioprotective, antiplatelet, anti-inflammatory effects. The flavonoid quercetin belongs to the aglycones of many plant flavonoid glycosides of higher plants. Some pharmacological effects of quercetin have been discovered recently, but the prospects of its use in veterinary medicine are being intensively studied, given its promising positive effects on the animals` body. The bioavailability of quercetin is highest in combination with pectin and insoluble oligosaccharides, which is associated with changes in the quantitative and qualitative composition of the mammals’ intestinal micro flora. A prerequisite for the development and testing of new veterinary medicinal products in accordance with the requirements of the international community for the registration of medicinal products is the conduct of clinical trials in compliance with the requirements of "Good Clinical Practice" (GCP). The introduction of biologically active drugs in the practice of veterinary medicine requires careful researches on target species of animals. Therefore, to assess the tolerability, safety and efficiency of the drug containing quercetin, a comprehensive clinical trial was performed on small pets (dogs). The study was conducted in veterinary clinics on healthy dogs of all ages, genders and breeds. The drug was used at a dose of 4 mg/kg body weight orally with food for 30 days. Blood sampling in test animals was performed before drug use and on days 30 and 60 of the experiment. The effect of the drug on the body was evaluated by clinical indicators, hematological and biochemical parameters of dogs' blood. Stabilized EDTA blood was used for morphological studies and blood serum for biochemical studies. According to the evaluation of the animals’ clinical condition and the obtained data of laboratory studies, it was found that the tolerance of the studied drug, administered orally to healthy dogs, was good. According to the results of comparison of clinical, hematological and biochemical parameters on the 30th and 60th day of the study with the initial data obtained before the use of the study drug, no negative changes were detected. Hematological studies indicated the activation of hematopoietic processes, normalization of leukogram parameters on 30th day after the drug application. Changes in the lymphocytes` content in the leukogram and the γ-globulin fraction in the protein-gram indicated a strengthening of dogs` immune protection. The results of biochemical studies of dogs's blood on the 30th day showed a reliable increase, within normal limits, in glucose, creatinine contents, increased catalase and alanine aminotransferase activity and decreased gamma-glutamyltransferase activity and the contents of diene conjugates and malonic dialdehyde. Activation of protein metabolism was established, which was indicated by a reliable increase in the total serum protein content and albumin content in the dogs` proteinogram. On the 60th day of the drug application no significant changes in hematological and biochemical parameters of the blood of dogs were detected. There was a slight decrease in the activity of catalase and SOD and a decrease in the content of malonic dialdehyde in the serum of dogs, compared with the values on the 30th day. This, in turn, showed a decrease in the intensity of the formation of toxic compounds in animals and pronounced antioxidant properties of the drug Hepanephran.

Prospects for the use of calcium ammonium nitrate on acidic soils of the Non-Black Earth Area

The results of the effectiveness of the application of calcium ammonium nitrate (CAN) in winter wheat crops of the “Moskovskaya-39” variety on the dark-gray forest, heavy-loamy soil in the Ryazan region is presented. The advantage of CAN-a a universal form of nitrogen fertilizer, which has a neutral reaction and creates optimal conditions for feeding plants with nitrogen in the zone of its application, where the main mass of plant roots is located, is revealed. Along with nitrogen, fertilizer contains calcium and magnesium, which are essential for the formation of winter wheat productivity, the exchange of carbohydrates and proteins. In the field experience with winter wheat on soils not saturated with bases, the results were obtained, indicating the effectiveness of a physiologically neutral form of nitrogen fertilizer, which showed an advantage over physiologically acidic fertilizer - ammonia nitrate. Dynamics of the formation of the productivity of sowing showed that the area of leaves reached the maximum sizes by the flowering phase of 61.6-76.6 cm2/plant, the largest value of the indicator 76.6 cm2/plant was in the variant with the use of CAN feeding at a dose of 54 kg/ha of nitrogen. The use of CAN provided a reliable increase in the harvest of winter wheat grain in comparison with ammonium nitrate. The grain harvest in the experiment was 63.7 - 67.1 cents per hectare, statistically close were the values of yield in variants using CAN in doses of 68 and 54 kg/ha of nitrogen, the increase in the harvest was 3.8-5.3% respectively. The creation of optimal conditions for winter wheat during the growing season was accompanied by an improvement in the quality of the grain, in comparison with the variant with the use of ammonium nitrate.

Study of the effect of active coal feed additive on the productivity of meat geese

The study of new effective fodder additives produced from byproducts of various industries, including the timber processing industry, is a very relevant issue. Research has been carried out on the study of active coal fodder phytoaddition in feedstuffs for geese fed to meat. According to the results of the scientific experiment, it was established that when introducing the tested active coal fodder additive in the diets of young geese of the Lindov breed, a positive tendency to increase the live weight of poultry, gross and average daily increases was noted, feed costs per 1 kg of increase in live weight were reduced, slaughter yield increased, and the level of profitability of growing geese per meat increased. Reliable increase of mass of muscular tissue of geese in case of slaughter in the second group by 3.3% has been established.

Influence of vegetation treatment with growth stimulants and protection agents on seed productivity of meadow fescue Nadezhda

The article provides information about the yield, development of leaf-stem diseases, as well as the biological effectiveness of drugs on meadow fescue crops. The purpose of research is to study the influence of protective agents and growth stimulants that provide the most complete realization of the potential of seed productivity of meadow fescue. After applying protection products, crop structure for 2018–2019 Treatment of plants for vegetation in the phase of entering the tube – the beginning of earing, the following drugs were used: “Fitolavin” – 1.5 l/ha; “Lariksin” – 50 g/ha; “Kolosal’ Pro” – 0.5 l/ha; “Strekar” – 1.5 l/ha; “Fitolavin” – 1.5 l/ha + “Kolosal’ Pro” – 0.5 l/ha. Research methodology and methods. The experience consists of 18 plots. The total area of one plot is 42 m2, the accounting area of one plot is 28 m2. Plots are placed – blockwise, repetitions are rendomized, the repetition is 3-fold. Observations and studies were conducted according to the generally accepted methodology. Meteorological conditions in 2018–2019 significantly varied from the long-term average. The hydrothermal coefficient (GTC) for the growing season in 2018 was 1.4; in 2019, it was 1.77, which is higher than the long – term average (1.57). Results. The development of the disease on average for all tiers of leaves for two years (2018–2019) in the control was 23.7 %, the prevalence – 67.5 %. In the variants with fungicides and biologics, the development and prevalence of leaf-stem diseases ranged from 9.4 to 17.4 % and from 32.4 to 56.5 %, respectively. The use of plant protection products led to a decrease in the development of diseases by 1.4–2.5 times (by 6.3–14.3 %), and the prevalence – by 1.2–2.1 times (by 11.0–35.1 %).The biological effectiveness of plant protection products ranged from 26.6 to 60.3 %. The applied preparations on average for two years of research provided a reliable increase in seed yield from 0.11 to 0.26 t/ha (from 32 to 76 %). Scientific novelty. The increase in seed productivity according to two-year data (2018–2019) was obtained due to a significant increase in all components of the yield structure.

STUDY OF IRRITANT AND SKIN-RESORPTIVE ACTION OF SODIUM-CONTAINING DRUG

Sodium-containing preparations have antimicrobial, anti-inflammatory and wound-healing activity, which determines their use in veterinary medicine. This effect is due to a wide range of physiological effects of sodium ions on animals metabolism. The article presents the results of experimental studies of the irritant effect of sodium-containing drugs: known – 2 % glycetinate, its analogues – 2 % ovocid, 3 % ovocid, 1.5 % potassium-sodium drug (Na / K, 1.5%). Studies of irritant and skin-resorptive action of drugs were performed by immersing the tail of animals in a test tube with the substance on white rats weighing 250–270 g. Four groups of rats were formed with 3 animals each: rats of I group (control) tails were immersed in test tubes with 2 % glycetinate, animals of II group – in test tubes with 2% oocide, III group – in test tubes with 3 % oocide, and in IV group rats – with Na / K, 1.5% drug. The exposure time was 4 hours. The animals were subjected to clinical observations, assessing their general condition and the reaction of the tail’s skin. The effect of drugs on the morphological and biochemical parameters of the rats blood was studied. The study of the local irritant effect of the studied drugs in rats from I, II and III groups showed a moderate local irritant reaction, as evidenced by the appearance of redness and thickening of the skin, and in group IV animals appeared only slight redness on the skin of the tails. This indicates a weaker local irritant effect of potassium-sodium-containing drug (1.5% K, Na), compared with other studied drugs. According to hematological studies, in the rats’s blood of groups III and IV, a reliable increase, within normal limits, the number of leukocytes and a decrease in hemoglobin content, compared with those of animals of group I. It was found that at 4 hours of the experiment under the action of the studied potassium-sodium drugs increases the permeability of Sodium and Potassium through the rats’s skin into the blood. In particular, in serum of animals from groups II and IV there was a reliable increase of Potassium content by 2.1 times, respectively, and a significant increase in Sodium content in the blood of animals from groups III and IV, compared with control group. In animals of groups II and IV there was a reliable increase of serum aspartate aminotransferase activity, and in rats of group III – increase of serum alanine aminotransferase, alkaline phosphatase activity and in triacylglycerol’s content, compared with rats of group I, which indicates the effect of 2 % glycecin analogues on metabolic processes in animals.