Effect of Sodium Hyaluronate on Inflammatory Cytokine Levels in Synovial Fluid of Patients with Knee Osteoarthritis

Background/Aims: The bradykinin B2 receptor (BDKRB2) +9/-9 gene polymorphisms have been shown to be associated with the susceptibility and severity of osteoarthritis (OA); however, the underlying mechanisms are unclear. In this study, we investigated the correlation between the BDKRB2 +9/-9 polymorphisms and pro-inflammatory cytokine levels in OA and the molecular mechanisms involved. Methods: A total of 156 patients with primary knee OA and 121 healthy controls were enrolled. The BDKRB2 +9/-9 polymorphisms were genotyped. The tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-8 levels were determined using Enzyme-linked immunosorbent assay (ELISA). The toll-like receptor (TLR)-2 and TLR-4 mRNA levels were determined by quantitative real-time PCR. The basal and bradykinin-stimulated pro-inflammatory cytokine secretion in human OA synoviocytes and the involvement of TLR-2 and mitogen-activated protein kinases (MAPKs) were investigated. Results: The presence of -9 bp genotype is associated with higher TNF-α, IL-6, and IL-8 levels and higher TLR-2 expression in OA patients. The basal and bradykinin-induced TLR-2 expressions in human OA synoviocytes were significantly reduced by specific inhibitors of p38, JNK1/2, and ERK1/2. Both the B2 receptor antagonist MEN16132 and TLR-2 silencing inhibited IL-6 and IL-8 secretion in human OA synoviocytes. Conclusion: The data suggested that the BDKRB2 +9/-9 polymorphisms influence pro-inflammatory cytokine levels in knee osteoarthritis by altering TLR-2 expression.

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Effects of exercise therapy on knee joint function and synovial fluid cytokine levels in patients with knee osteoarthritis

Molecular Medicine Reports ◽

10.3892/mmr.2012.1168 ◽

2012 ◽

Vol 7 (1) ◽

pp. 183-186 ◽

Cited By ~ 19

Author(s):

SHAO-LAN ZHANG ◽

HONG-QI LIU ◽

XIAO-ZU XU ◽

JUAN ZHI ◽

JIAO-JIAO GENG ◽

...

Keyword(s):

Synovial Fluid ◽

Knee Joint ◽

Knee Osteoarthritis ◽

Exercise Therapy ◽

Joint Function ◽

Cytokine Levels

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Inflammatory cytokine levels in synovial fluid 3, 4 days postoperatively and its correlation with early-phase functional recovery after anterior cruciate ligament reconstruction: a cohort study

Journal of Experimental Orthopaedics ◽

10.1186/s40634-016-0067-z ◽

2016 ◽

Vol 3 (1) ◽

Cited By ~ 4

Author(s):

Makiko Inoue ◽

Takeshi Muneta ◽

Miyoko Ojima ◽

Kaori Nakamura ◽

Hideyuki Koga ◽

...

Keyword(s):

Anterior Cruciate Ligament ◽

Cohort Study ◽

Synovial Fluid ◽

Anterior Cruciate Ligament Reconstruction ◽

Inflammatory Cytokine ◽

Ligament Reconstruction ◽

Cruciate Ligament ◽

Cruciate Ligament Reconstruction ◽

Cytokine Levels ◽

Anterior Cruciate

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Clinical Therapy of Shujin Huoxue Tablet Combined with Sodium Hyaluronate on Inflammatory Factors of Synovial Fluid (IL-6, TNF-α) in the Treatment of Knee Osteoarthritis: Clinical Trial Study

Pakistan Journal of Zoology ◽

10.17582/journal.pjz/20200418160406 ◽

2020 ◽

Author(s):

Guangyu Wang

Keyword(s):

Clinical Trial ◽

Synovial Fluid ◽

Knee Osteoarthritis ◽

Sodium Hyaluronate ◽

Inflammatory Factors ◽

Clinical Therapy ◽

Tnf Α

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Influence of Isometric Exercise Combined With Electromyostimulation on Inflammatory Cytokine Levels, Muscle Strength, and Knee Joint Function in Elderly Women With Early Knee Osteoarthritis

Frontiers in Physiology ◽

10.3389/fphys.2021.688260 ◽

2021 ◽

Vol 12 ◽

Author(s):

Sunhee Park ◽

Sukyung Min ◽

Si-Hwa Park ◽

Jaehyun Yoo ◽

Yong-Seok Jee

Keyword(s):

Muscle Strength ◽

Knee Osteoarthritis ◽

Inflammatory Cytokine ◽

Elderly Women ◽

Isometric Exercise ◽

Knee Function ◽

Whole Body ◽

Controlled Study ◽

Knee Oa ◽

Cytokine Levels

Background and Objectives: Muscle strengthening exercise is suggested to beneficial for patients with knee osteoarthritis (OA) and electrical muscular stimulation is reported to be effective in improvement of muscle strength. This study examined whether isometric exercise combined with whole body-electromyostimulation (WB-EMS) can improve serum cytokine levels, muscle strength, and knee function in elderly women with early knee OA.Materials and Methods: This randomized controlled study included 75 participants assigned into three groups: the control group (CON), isometric exercise group (ISOM), and isometric exercise and electromyostimulation group (ISOM + EMS). The two exercise groups performed their respective programs for 8 weeks, 3 days a week, 30 min a day. The main exercises for both groups were performed continuously during the 20 min in an alternation of a 6-s contraction with a 4-s break. At pre- and post-intervention, anthropometric variables, muscle strength, Knee Injury and Osteoarthritis Outcome Score (KOOS), and blood sampling for biomarkers including interleukin-6, tumor necrosis factor-α, C-reactive protein, and resistin were performed.Results: All variables at pre-intervention showed no significant differences among the three groups. However, there were significant differences between groups for body composition, muscle strength, KOOS subscale scores, and biomarkers. ISOM + EMS group resulted in a significant reduction in body weight, fat mass, fat percentage, inflammatory cytokine levels, and increased muscle strength. An ISOM + EMS group had the best KOOS score among all groups.Conclusion: Isometric exercise combined with WB-EMS resulted in the best overall improvements in knee function and alleviating the pain and symptoms of patients with early knee OA. Further, reduced levels of inflammatory cytokines were observed. These non-pharmacologic, non-invasive interventions should be considered by healthcare specialists for elderly patients with early knee OA.

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