scholarly journals УСКОРЕННЫЙ МЕТОД ПОДСЧЕТА МЕЗОФИЛЬНЫХ АЭРОБНЫХ И ФАКУЛЬТАТИВНО-АНАЭРОБНЫХ МИКРООРГАНИЗМОВ В ПТИЦЕПРОДУКТАХ

2019 ◽  
Author(s):  
S.S. Kozak ◽  
V.G. Semenov ◽  
R.T. Abdraimov ◽  
Y.A. Kozak
Keyword(s):  
Nutrient Medium ◽  
Classical Method ◽  
Raw Materials ◽  
High Sensitivity ◽  
Food Samples ◽  
Water Soluble ◽  
Gelling Agent ◽  
Comparative Tests

Проведена апробация и произведены сравнительные испытания тест-пластин, предназначенных для подсчета количества мезофильных аэробных и факультативно-анаэробных микроорганизмов (КМАФАнМ) в птицепродуктах. Лабораторные испытания включали в себя выявление диапазонов чувствительности тест-пластин для определения КМАФАнМ, подсчет процента их извлекаемости ( всхожести) с поверхности тест-пластин, а также проведение их сравнительных испытаний с помощью классического метода анализа. Тест-пластины типа 1 содержат готовую питательную среду, гель и тетразолиевый индикатор используются для подсчета КМАФАнМ в образцах пищевых продуктов, продовольственном сырье, а также при мониторинге окружающей среды. Тест-пластины типа 2 предназначены для экспрессного подсчета КМАФАнМ в пищевых продуктах и объектах внешней среды, содержат готовую питательную среду, водорастворимый желирующий агент, цветной индикатор. При сравнении чувствительности классических сред и тест-пластин типа 1 и типа 2 при определении КМАФАнМ была установлена высокая чувствительность как питательного агара, так и испытуемых тест-пластин. Для плотных сред она составляет не менее 0,1 мл суспензии из 6-го разведения. В наших исследованиях на всех чашках и тест-пластинах из 6 и 7 разведений выявлен рост типичных колоний, что свидетельствует о высокой чувствительности. Установлено, что процент извлекаемости на тест-пластинах хороший: на тест-пластинах типа 1 98,9, на тест-пластинах типа 2 86,8. При сравнительном исследовании классического метода и тест-пластин был получен хороший результат. Во всех случаях результаты исследований на КМАФАнМ тест-пластин сопоставим с классическим методом: процент извлекаемости апробируемого материала составил более 80, в некоторых случаях даже 100. Тест-пластины для определения КМАФАнМ просты в использовании, надежны, имеют длительный срок хранения (1 год), с их применением уменьшаются сроки проведения анализа, уменьшается трудоемкость исследований.Approbation and comparative tests of test-plates designed to count the number of mesophilic aerobic and facultative anaerobic microorganisms (NMAFAnM) in poultry products were carried out. Laboratory tests included identifying the sensitivity ranges of test plates for determining NMAFAnM, calculating the percentage of their recoverability ( germination) from the surface of the test-plates, as well as conducting their comparative tests using the classical analysis method. Type 1 test-plates contain ready-made nutrient medium, gel and tetrazolium indicator they are used to calculate NMAFAnM in food samples, food raw materials, and also when monitoring the environment. Type 2 test-plates are intended for express counting of NMAFAnM in food products and environmental objects they contain ready-made nutrient medium, a water-soluble gelling agent and a color indicator. When comparing the sensitivity of classical media and type 1 and type 2 test-plates when determining NMAFAnM, a high sensitivity of both nutrient agar and test test-plates was established. For solid media, it is at least 0.1 ml of a suspension of 6 dilutions. In our studies, the growth of typical colonies was revealed on all plates and test-plates from 6 and 7 dilutions, which indicates high sensitivity. The recovery rate on the test-plates was found to be good: 98.9 on type 1 test-plates and 86.8 on type 2 test-plates. In a comparative study of the classical method and test-plates, a good result was obtained. In all cases, the results of studies on NMAFAnM test-plates are comparable with the classical method: the percentage of extractability of the tested material was more than 80, in some cases even 100. Test-plates for determining NMAFAnM are easy to use, reliable, have a long shelf life (1 year), with their use, the analysis time is reduced, and the complexity of research is reduced.

Angiotensin II-induced thirst, but not sodium appetite, via AT1 receptors in organum cavum prelamina terminalis

1995 ◽  
Vol 268 (6) ◽  
pp. R1401-R1405 ◽  
Author(s):  
M. el Ghissassi ◽  
S. N. Thornton ◽  
S. Nicolaidis
Keyword(s):  
Angiotensin Ii ◽  
Salt Intake ◽  
High Sensitivity ◽  
Ang Ii ◽  
Nacl Solution ◽  
At1 Receptors ◽  
Sodium Appetite ◽  
Specific Antagonist

The angiotensin receptor specificity, with respect to fluid intake, of the organum cavum prelamina terminalis (OCPLT), a recently discovered discrete forebrain structure with high sensitivity to angiotensin II (ANG II), was investigated. ANG II (10 ng) microinjected into the OCPLT significantly increased water consumption but did not induce intake of a hypertonic (3%) NaCl solution. Losartan, an ANG II type 1 (AT1) receptor-specific antagonist, produced dose-related (1-100 ng) inhibition of ANG II-induced drinking. The ANG II type 2 receptor-specific antagonist CGP-42112A was ineffective. Intake of the 3% NaCl solution in response to microinjection of either of the antagonists into the OCPLT was never observed. These findings suggest that water intake produced by microinjection of ANG II into the OCPLT is mediated by AT1 receptors uniquely and that, in contrast to other regions of the brain, these receptors do not induce salt intake when stimulated by ANG II.

Evaluation of Dry Sheet Medium Culture Plate (Compactdry TC) Method for Determining Numbers of Bacteria in Food Samples

2000 ◽  
Vol 63 (5) ◽  
pp. 665-667 ◽  
Author(s):  
SHINGO MIZUOCHI ◽  
HIDEMASA KODAKA
Keyword(s):  
Cold Water ◽  
Correlation Coefficients ◽  
Food Samples ◽  
Water Soluble ◽  
Gelling Agent ◽  
Plate Count ◽  
Aerobic Bacteria ◽  
Suitable Alternative ◽  
Standard Plate Count ◽  
Standard Plate

The Compactdry, a ready-to-use and self-diffusible dry medium sheet culture system, has been developed by the Nissui Pharmaceutical Co. Ltd. for enumerating bacteria in food. The Compactdry consists of special spread sheet with culture medium that is the same as standard method nutrients, a cold water-soluble gelling agent, and a unique plastic dish. The procedure for bacterial examination in a sample solution (1 ml) is to just inoculate a test solution into the center of the self-diffusible medium and incubate at 35°C for 48 h. The Compactdry TC (CTC) for the enumeration of total aerobic bacteria from 97 food samples was compared with the standard plate count (SPC) method and 3M Petrifilm aerobic count plates (PAC). The correlation coefficients between the CTC and SPC method, the CTC and PAC, and the PAC and SPC method were 0.97, 0.99, and 0.97, respectively. The Compactdry system is useful for the enumeration of total aerobic bacteria in food and may be a possible suitable alternative to the conventional pour-plate or the Petrifilm plate methods.

scholarly journals No additional value of conventional and high-sensitivity cardiac troponin over clinical scoring systems in the differential diagnosis of type 1 vs. type 2 myocardial infarction

2018 ◽  
Vol 56 (5) ◽  
pp. 702-709 ◽  
Author(s):  
Luciano Consuegra-Sánchez ◽  
Juan José Martínez-Díaz ◽  
Luis García de Guadiana-Romualdo ◽  
Samantha Wasniewski ◽  
Patricia Esteban-Torrella ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Cardiac Troponin ◽  
Troponin I ◽  
Troponin T ◽  
Characteristic Curve ◽  
High Sensitivity ◽  
St Segment ◽  
Clinical Scoring

AbstractBackground:The distinction of type 1 and type 2 myocardial infarction (MI) is of major clinical importance. Our aim was to evaluate the diagnostic ability of absolute and relative conventional cardiac troponin I (cTnI) and high-sensitivity cardiac troponin T (hs-cTnT) in the distinction between type 1 and type 2 MI in patients presenting at the emergency department with non-ST-segment elevation acute chest pain within the first 12 h.Methods:We measured cTnI (Dimension Vista) and hs-cTnT (Cobas e601) concentrations at presentation and after 4 h in 200 patients presenting with suspected acute MI. The final diagnosis, based on standard criteria, was adjudicated by two independent cardiologists.Results:One hundred and twenty-five patients (62.5%)were classified as type 1 MI and 75 (37.5%) were type 2 MI. In a multivariable setting, age (relative risk [RR]=1.43, p=0.040), male gender (RR=2.22, p=0.040), T-wave inversion (RR=8.51, p<0.001), ST-segment depression (RR=8.71, p<0.001) and absolute delta hs-cTnT (RR=2.10, p=0.022) were independently associated with type 1 MI. In a receiver operating characteristic curve analysis, the discriminatory power of absolute delta cTnI and hs-cTnT was significantly higher compared to relative c-TnI and hs-cTnT changes. The additive information provided by cTnI and hs-cTnT over and above the information provided by the “clinical” model was only marginal.Conclusions:The diagnostic information provided by serial measurements of conventional or hs-cTnT is not better than that yielded by a simple clinical scoring model. Absolute changes are more informative than relative troponin changes.

scholarly journals Comparative Study of the Effect of Various Forms of Quercetin on Experimental Diabetes

2018 ◽  
Vol 25 (4) ◽  
pp. 383-388
Author(s):  
Kateryna Posokhova ◽  
Iryna Stechyshyn ◽  
Inna Krynytska ◽  
Mariya Marushchak ◽  
Inna Birchenko ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 1 Diabetes ◽  
Comparative Study ◽  
Experimental Diabetes ◽  
Glycosylated Hemoglobin ◽  
Water Soluble ◽  
Soluble Form

Abstract Background and aims: Diabetes mellitus (DM) is a multifactorial metabolic disorder characterized by hyperglycaemia caused by insulin deficiency or insulin resistance. It is a global public health problem. This study aimed to determine specific pharmacological effect of quercetin in water soluble and liposomal preparations in experimental diabetes mellitus. Material and methods: We examined the effect of Corvitin and Lipoflavone (at the dose of 10 mg / kg body weight) in a comparative study in white rats with type 1 diabetes and type 2 diabetes coupled with obesity. To simulate the forms of diabetes mellitus most analogous to those in humans we used Streptozotocin at the doses of 30 mg / kg and 50 mg / kg. We tested the levels of glucose, glycosylated hemoglobin, C-reactive protein, and interleukins 6 and 4 in the blood. Results: In animals with type 1 and type 2 diabetes Lipoflavone significantly reduces glucose and glycosylated hemoglobin levels compared to the rats treated with Corvitin. When administered to animals with diabetes, the effect of quercetin in liposomal form on the concentrations of IL-6, IL-4 and Creactive protein is also larger compared to the water-soluble form. Conclusions: Water soluble quercetin preparation Corvitin and to a larger extent liposomal preparation of this flavonoid, Lipoflavone, show anti-inflammatory effect and restore key parameters of carbohydrate metabolism in experimental type 1 diabetes mellitus and type 2 diabetes coupled with obesity, reducing blood glucose and glycosylated hemoglobin levels.

P1730Serial high-sensitivity troponin I measurements to discriminate type 2 from type 1 myocardial infarction

2019 ◽  
Vol 40 (Supplement_1) ◽  
Author(s):  
D A Psyrakis ◽  
J Bormann ◽  
B Von Jeinsen ◽  
J S Wolter ◽  
M Weferling ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Troponin I ◽  
High Sensitivity ◽  
High Morbidity ◽  
Absolute Change ◽  
Coronary Syndrome ◽  
High Sensitivity Troponin ◽  
Rule Out

Abstract Background Acute myocardial infarction (MI) is associated with high morbidity and mortality. A robust differentiation between type 1 and type 2 MI (T1/T2MI) has prognostic and therapeutic implications. We investigated whether serial high-sensitivity cardiac troponin I measurements could reliably discriminate T1MI from T2MI in patients presenting with a non-ST elevation myocardial infarction (NSTEMI). Methods We used data from a prospective acute coronary syndrome biomarker registry of patients with suspected MI that presented at or were transferred to one of two study centres. Here, we analysed an unselected group of 265 NSTEMI patients (67.2% males). Blood was drawn on admission and after 3 hours. High-sensitivity troponin I (hs-cTnI) was measured in frozen samples by a technician blinded to patient characteristics. T1MI or T2MI was defined as the gold-standard study diagnosis by two independent cardiologists based on all available data according to the Third Universal Definition of MI. Results A diagnosis of T2MI was made in 55 patients (20.8%) in the NSTEMI cohort. T2MI patients did not differ from T1MI patients regarding age, gender, traditional risk factors, or percentage of those with a history of coronary artery disease. Median baseline hs-cTnI levels were higher in T1MI (436.25; IQR 63.7–1918.8 ng/L) than in T2MI patients (48.4; IQR 11.7–305.9 ng/L; p<0.001). Absolute change in hs-cTnI concentration between 0 and 3 h was greater in T1MI than in T2MI patients with Dhs-cTnI 93.6 ng/L (IQR 13.5–815.3 ng/L) vs. 20.4 ng/L (IQR 2.5–106.5 ng/L) (p<0.001). hs-cTnI yielded an area under the receiver operator characteristics (AUROC) curve for identifying T2MI at baseline of 0.71 (IQR 0.64–0.79) and after 3 h of 0.7 (IQR 0.61–0.78).Dhs-cTnI was associated with an AUROC of 0.68 (IQR 0.6–0.76). Regarding a rule-out approach, Youden-optimized cut-offs for hs-cTnI at baseline as well as for the absolute change in hs-cTnI concentration were calculated (186.5 ng/L; 154.4 ng/L). Use of these two criteria yielded a sensitivity of 89% (78–96%) and a negative predictive value of 95% (89–98%) to exclude T2MI. 49 of 55 T2MI patients would have been ruled out using this algorithm. Conclusion Our data show that hs-cTnI concentrations differ between patients presenting with T1 and T2MI. The concentration of hs-cTnI and its change over time has the potential to rule out T2MI and therefore to identify patients who might benefit from an early invasive management. The differentiation between T1MI and T2MI by using hs-cTnI is nevertheless challenging, and further research on specific algorithms is needed. Acknowledgement/Funding 3German Center for Cardiovascular Research (DZHK), Partnersite Rhein Main, Bad Nauheim, Germany

Multibiomarker model to discriminate Type 1 and Type 2 myocardial infarction

2020 ◽  
Vol 41 (Supplement_2) ◽  
Author(s):  
J Neumann ◽  
N.A Soerensen ◽  
T.S Hartikainen ◽  
P.M Haller ◽  
J Lehmacher ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Emergency Department ◽  
Troponin I ◽  
External Validation ◽  
Area Under The Curve ◽  
High Sensitivity ◽  
Funding Source ◽  
Multivariable Logistic Regression Model

Abstract Background The discrimination of patients with type 1 myocardial infarction (T1MI) from patients with type 2 MI (T2MI) is often challenging in the emergency department. Earlier we presented a discrimination model, which based on clinical variables, as well as on troponin concentrations. In the present analyses we sought to investigate the discriminative power of 28 biomarkers in patients with T1MI and T2MI. Methods Patients presenting to the emergency department with symptoms suggestive of MI were recruited. The final diagnosis of all patients was adjudicated by two physicians in a blinded fashion and based on the fourth universal definition of MI. For the present analyses only patients with T1MI and T2MI were used. In total 28 biomarkers were measured in blood samples collected directly at admission. A multivariable logistic regression model for T1MI vs T2MI as the dependent variable was used and the predictors were chosen via backward step-down selection. Results In total 138 patients (107 T1MI and 31 T2MI) were available for the analyses. The median age of the study population was 65 years and 66.7% were males. Hypertension was present in 77.4% and dyslipidemia in 41.3%. In the multivariable model four biomarkers (apolipoprotein A-II, n-terminal prohormone of brain natriuretic peptide, copeptin and high-sensitivity troponin I) were significant discriminators between T1MI and T2MI (Table 1). Internal validation of the model via bootstrap shows a for overoptimism corrected area under the curve of 0.82. Conclusion Using a multibiomarker approach discrimination between T1MI and T2MI could be improved. External validation of our findings is warranted. Funding Acknowledgement Type of funding source: Public grant(s) – National budget only. Main funding source(s): Research fellowship by the Deutsche Forschungsgemeinschaft

PO159 Mortality at 4 Years In Patients With Type 2 MI Compared to Type 1 MI and Stable High Sensitivity Troponin T Elevations

Global Heart ◽  
2018 ◽  
Vol 13 (4) ◽  
pp. 416
Author(s):  
J.K. French ◽  
A. Etaher ◽  
O. Gibbs ◽  
Y. Saad ◽  
S. Frost ◽  
...  
Keyword(s):  
Troponin T ◽  
High Sensitivity ◽  
High Sensitivity Troponin ◽  
High Sensitivity Troponin T

scholarly journals Fabrication of Gas-Sensor Chips Based on Silicon–Carbon Films Obtained by Electrochemical Deposition

Chemosensors ◽  
2019 ◽  
Vol 7 (4) ◽  
pp. 52 ◽  
Author(s):  
Tatiana N. Myasoedova ◽  
Mikhail N. Grigoryev ◽  
Nina K. Plugotarenko ◽  
Tatiana S. Mikhailova
Keyword(s):  
Gas Sensor ◽  
Electrochemical Deposition ◽  
Recovery Time ◽  
Carbon Film ◽  
High Sensitivity ◽  
Carbon Films ◽  
Silicon Carbon ◽  
Response And Recovery

In this study, we designed two types of gas-sensor chips with silicon–carbon film, doped with CuO, as the sensitive layer. The first type of gas-sensor chip consists of an Al2O3 substrate with a conductive chromium sublayer of ~10 nm thickness and 200 Ω/□ surface resistance, deposited by magnetron sputtering. The second type was fabricated via the electrochemical deposition of a silicon–carbon film onto a dielectric substrate with copper electrodes formed by photoelectrochemical etching. The gas sensors are sensitive to the presence of CO and CH4 impurities in the air at operating temperatures above 150 °C, and demonstrated p- (type-1) and n-type (type-2) conductivity. The type-1 gas sensor showed fast response and recovery time but low sensitivity, while the type-2 sensor was characterized by high sensitivity but longer response and recovery time. The silicon–carbon films were characterized by the presence of the hexagonal 6H SiC polytype with the impurities of the rhombohedral 15 R SiC phase. XRD analysis revealed the presence of a CuO phase.

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