scholarly journals Urine Culture Contamination Rates at a University Hospital

2021 ◽  
Vol 34 (3) ◽  
pp. 182-185
Author(s):  
Nuray Arı ◽  
Emine Yesilyurt Sölen ◽  
Neziha Yilmaz
Keyword(s):  
Urine Culture ◽  
University Hospital

URINARY INFECTION AT DEPARTMENT OF UROLOGY OF HUE UNIVERSITY OF MEDICINE AND PHARMACY HOSPITAL

2018 ◽  
pp. 100-108
Author(s):  
Dinh Khanh Le ◽  
Dinh Dam Le ◽  
Khoa Hung Nguyen ◽  
Xuan My Nguyen ◽  
Minh Nhat Vo ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urinary Tract Infections ◽  
Urine Culture ◽  
Clinical Manifestations ◽  
University Hospital ◽  
E Coli ◽  
Positive Urine ◽  
Tract Infections

Objectives: To investigate clinical characteristics, bacterial characteristics, drug resistance status in patients with urinary tract infections treated at Department of Urology, Hue University Hospital. Materials and Method: The study was conducted in 474 patients with urological disease treated at Department of Urology, Hue Universiry Hospital from July 2017 to April 2018. Urine culture was done in the patients with urine > 25 Leu/ul who have symptoms of urinary tract disease or infection symptoms. Patients with positive urine cultures were analyzed for clinical and bacterial characteristics. Results: 187/474 (39.5%) patients had symptoms associated with urinary tract infections. 85/474 (17.9%) patients were diagnosed with urinary tract infection. The positive urine culture rate was 45.5%. Symptoms of UTI were varied, and no prominent symptoms. E. coli accounts for the highest proportion (46.67%), followed by, Staphycoccus aureus (10.67%), Pseudomonas aeruginsa (8,0%), Streptococcus faecali and Proteus (2.67%). ESBL - producing E. coli was 69.23%, ESBL producing Enterobacter spp was 33.33%. Gram-negative bacteria are susceptible to meropenem, imipenem, amikacin while gram positive are vancomycin-sensitive. Conclusions: Clinical manifestations of urinary tract infections varied and its typical symptoms are unclear. E.coli is a common bacterium (46.67%). Isolated bacteria have a high rate of resistance to some common antibiotics especially the third generation cephalosporins and quinolones. Most bacteria are resistant to multiple antibiotics at the same time. Gram (+) bacteria are susceptible to vancomycin, and gram (-) bacteria are susceptible to cefoxitin, amikacin, and carbapenem. Key words: urinary tract infection

scholarly journals Prevalence of asymptomatic bacteriuria among low risk pregnant women attending antenatal care clinic in tertiary hospital: a cross sectional study

2019 ◽  
Vol 8 (7) ◽  
pp. 2742
Author(s):  
Ahmed M. Abbas ◽  
Moustafa Kamel ◽  
Mohammed Z. Abdelrahman ◽  
Ahmed A. Youssef ◽  
Mohammed A. Youssef
Keyword(s):  
Antenatal Care ◽  
Pregnant Women ◽  
Urine Culture ◽  
Asymptomatic Bacteriuria ◽  
Antibiotic Sensitivity ◽  
Low Risk ◽  
University Hospital ◽  
High Power Field ◽  
Cross Sectional ◽  
Care Clinic

Background: The current study aims to identify the prevalence of asymptomatic bacteriuria among low-risk pregnant women attending the antenatal care clinic of tertiary University Hospital and to detect the most common causative organisms.Methods: A prospective cross-sectional study conducted at a tertiary University Hospital in the period between 1st November 2017 and 31st June 2018. All eligible women attending the outpatient clinic for antenatal care were approached to participate in the study. The recruited women were assessed through detailed history and clinical examination. Urine samples were taken for culture and sensitivity within two hours, in order to avoid bacterial multiplication. Urine culture and antibiotic sensitivity test were done.Results: The study included 250 women. The mean age of included women was 25.89±5.49 (18-42 years). The urine analysis results showed that 32% of cases had pus cells >5 per high power field and 10.4% of cases have protein (+). Casts were present in 0.8% of cases. RBCs >5 per high power field were present in 4.8% of cases. ASB is defined as urine culture with more than 100,000 colony forming unit; this was present in 30 cases. This indicates that the prevalence of ASB among studied cases was 12%. Enterococcus was the most prevalent organism as it was present in 36.7% among positive cases. Antibiotic sensitivity test was performed to the growing organisms indicated that teicoplanin, imipenem, cefoxitin, cefotaxime + clavulanic acid, Entrapenem, and trimethoprim + sulfamethoxazole are the most effective antibiotics against the most common organisms causing asymptomatic bacteriuria.Conclusions: The prevalence of asymptomatic bacteriuria among low-risk pregnant women is about 12%. Urine culture for asymptomatic bacteriuria should be the standard of care for evaluation of pregnant patient during antenatal care as regard presence or absence of UTI.

scholarly journals Comparison of Urine Flow Cytometry on the UF-1000i System and Urine Culture of Urine Samples from Urological Patients

2021 ◽  
pp. 1-10
Author(s):  
Moritz Fritzenwanker ◽  
Marcel Oliver Grabitz ◽  
Borros Arneth ◽  
Harald Renz ◽  
Can Imirzalioglu ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Urine Culture ◽  
Pearson Correlation ◽  
Urine Flow ◽  
Urine Samples ◽  
University Hospital ◽  
Predictive Values ◽  
Colony Forming Units ◽  
Significant Bacteriuria ◽  
Urine Flow Cytometry

<b><i>Introduction:</i></b> The aims of this study were to evaluate urine flow cytometry (UFC) as a tool to screen urine samples of urological patients for bacteriuria and to compare UFC and dipstick analysis with urine culture in a patient cohort at a urological department of a university hospital. <b><i>Methods and Material:</i></b> We screened 662 urine samples from urological patients (75.2% male; 80.7% inpatients; mean age 58 years). UFC results were compared to microbiological urine culture. <b><i>Results:</i></b> The accuracy in using the UFC-based parameters for detecting cultural bacteriuria was 91.99% and 88.97% for ≥10<sup>5</sup> colony-forming units (CFU)/mL and ≥10<sup>4</sup> CFU/mL, respectively. UFC and leukocyte dipstick analysis measured leukocyturia similarly (Pearson correlation coefficient 0.87, <i>p</i> value &#x3c;0.01%), but dipstick analysis scored less accurately on bacteriuria (accuracy 59.37% and 62.69%). UFC remained effective in subgroup analysis of patients of both sexes and with different urological conditions with its overall use only slightly impaired when assessing gross hematuria (NPV 84.62% for ≥10<sup>4</sup> CFU/mL). UFC also reliably removed those urine samples below cutoffs with negative predictive values of 99.28% for ≥10<sup>5</sup> CFU/mL and 95.86% for ≥10<sup>4</sup> CFU/mL. <b><i>Conclusion:</i></b> Counting bacteria with UFC is an accurate and rapid method to determine significant bacteriuria in urological patients and is superior to dipstick analysis or indirect surrogate parameters such as leukocyturia. When UFC is available, we recommend it to be used for the diagnosis of bacteriuria over findings obtained by dipstick analysis.

scholarly journals Resensitization of Fluconazole-Resistant Urinary Candida spp. Isolates by Amikacin through Downregulation of Efflux Pump Genes

2020 ◽  
Vol 69 (1) ◽  
pp. 73-84
Author(s):  
EVA A. EDWARD ◽  
NELLY M. MOHAMED ◽  
AZZA S. ZAKARIA
Keyword(s):  
Efflux Pump ◽  
Urine Culture ◽  
University Hospital ◽  
Quantitative Real Time Pcr ◽  
Candida Spp ◽  
Recent Approach ◽  
Pump Activity ◽  
Fluconazole Resistant ◽  
Tract Infections ◽  
The Impact

The contribution of fluconazole-resistant Candida spp. isolates to urinary tract infections in Egypt has become a nationwide problem. A recent approach to overcome such disaster is combining conventional antifungals with non-antifungals. This study investigated the interaction of amikacin with fluconazole against resistant Candida strains isolated from the urine culture of patients admitted to Alexandria Main University Hospital. Among the collected Candida spp. isolates, 42.9% were resistant to fluconazole with MICs ranging between 128 and 1,024 μg/ml. The resistance-modifying activity of amikacin (4,000 μg/ml) was studied against fluconazole-resistant isolates where amikacin sensitized 91.7 % of resistant Candida spp. isolates to fluconazole with a modulation factor ranging between 32 and 256. The rhodamine efflux assay was performed to examine the impact of amikacin on efflux pump activity. After 120 minutes of treatment, amikacin affected the efflux pump activity of the isolates tested with a percentage of reduction in the fluorescence intensity of 8.9%. Quantitative real-time PCR was applied to assess the amikacin effect on the expression of the efflux pump genes MDR1, CDR1, and CDR2. The downregulatory effect of amikacin on the expression of the studied genes caused a percentage of reduction in the expression level ranging between 42.1 and 94%. In conclusion, amikacin resensitized resistant Candida spp. isolates to fluconazole and could be used in combination in the management of candiduria with a higher efficiency or at lower administration doses. To the best of our knowledge, this is the first study evaluating the enhancement of fluconazole activity in combination with amikacin against Candida spp.

scholarly journals Prevalência e perfil de sensibilidade de bactérias isoladas da urina de gestantes atendidas no serviço de obstetrícia de um hospital terciário

2016 ◽  
Vol 26 (4) ◽  
pp. 24071
Author(s):  
Fernanda Aguirre Carvalho ◽  
Mônica De Abreu Rodrigues ◽  
Angelita Bottega ◽  
Rosmari Hörner
Keyword(s):  
Urinary Tract Infection ◽  
Pregnant Women ◽  
Urine Culture ◽  
University Hospital ◽  
Gram Negative ◽  
Positive Urine ◽  
Amoxicillin Clavulanic Acid ◽  
The University ◽  
Santa Maria ◽  
Sensitivity Profile

Aims: To evaluate the prevalence of bacteria in urine cultures of pregnant women seen at the outpatient clinic of the Department of Obstetrics at the University Hospital of Santa Maria, Brazil, and to determine the antibiotic sensitivity profile of these bacteria.Methods: The reports issued by the Laboratory of Clinical Analysis of the University Hospital of Santa Maria were retrospectively analyzed. All positive urine cultures of pregnant women seen at the Department of Obstetrics from January to December 2014 were included in the study. The tests for the identification of bacterial isolates and their sensitivity profiles were assessed by an automated system.Results: A total of 423 positive urine cultures were detected in the pregnant women. Gram-negative Escherichia coli was the most prevalent microorganism (46.50%). Gram-positive Staphylococcus saprophyticus was the second most prevalent bacterium (6.2%). Candida spp. was isolated from 94 (21.8%) urine samples. Nitrofurantoin and amoxicillin/clavulanic acid showed the lowest antimicrobial resistance against E. coli (91.33% and 90.77%, respectively). Ampicillin had the highest sensitivity among prevalent Gram-positive bacteria.Conclusions: The sensitivity profile found in this study allows us to suggest nitrofurantoin and/or amoxicillin/clavulanic acid for the treatment of urinary tract infection caused by Gram-negative bacteria during pregnancy. Given the prevalence rates detected in this study, these antimicrobials can be initiated empirically before the urine culture results are known, in the cases of symptomatic urinary tract infection. This study underscores the importance of urine culture in the prenatal period and in the third trimester because of the different microorganisms identified and the different sensitivity to the antimicrobials tested.

scholarly journals 56 ‘A Closed-Loop Audit on The Appropriateness of Urine Testing Carried Out on Surgical Patients in The Acute Surgical Unit in University Hospital Limerick as Compared with Hospital Guidelines’

2021 ◽  
Vol 108 (Supplement_6) ◽  
Author(s):  
B Murphy ◽  
K Amin ◽  
N Ali
Keyword(s):  
Nursing Staff ◽  
Urine Culture ◽  
Antimicrobial Treatment ◽  
University Hospital ◽  
Education Session ◽  
High Turnover ◽  
Laboratory Staff ◽  
Urine Testing ◽  
Surgical Unit

Abstract Aim The acute surgical unit (ASU) in University Hospital Limerick (UHL) has a high turnover of patients of all surgical specialties, many of whom are sent home without the need for admission or follow-up. Urinalysis is very accessible, and is carried out on many patients, prior to them being seen by a doctor. Inappropriate urinalysis sets off a cascade of inappropriate urine culture, antimicrobial treatment and a waste of resources and time of nursing, medical and laboratory staff. We aim to identify and reduce inappropriate testing. Method The notes of all ASU patients during a one-week period were analysed using the ‘Therefore Navigator’ application, where all patient notes are scanned on leaving the ASU. Data relating to presenting complaint, presence or absence of urinary symptoms and abdominal pain, and urine dipstick and culture results (if performed) was collected for each patient. The results were compared with UHL guidelines on testing, an information sheet was designed and placed in ASU, and an education session for nursing staff was carried out. The second cycle was performed one month later. Results In the first cycle, 68% of the 99 ASU patients had urinalysis, and 48% had urine culture performed. Of cultures performed, only 23 of 47 (49%) were deemed indicated according to the guidelines. In the second cycle, 39% had urinalysis. 28% had urine cultured, 79% of these were deemed to have been indicated. Conclusions Inappropriate urine testing in the ASU decreased following teaching for nursing staff, together with an information leaflet being placed in the ASU.

scholarly journals Bacteriuria in Pregnancy in a Danish Contemporary Cohort of Women

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Vinnie H. Greve ◽  
Thomas Greve ◽  
Rikke B. Helmig
Keyword(s):  
Escherichia Coli ◽  
Pregnant Women ◽  
Urine Culture ◽  
University Hospital ◽  
Historical Cohort ◽  
Asian Origin ◽  
Fetal Medicine ◽  
Uropathogenic Bacteria ◽  
Mixed Ethnicity ◽  
In Pregnancy

Introduction. The purpose of this study is to describe bacteriuria with regard to the uropathogens found in relation to the frequency of urine culture tests in a contemporary cohort of pregnant Danish women. Methods. A historical cohort study of 24,817 pregnant women registered in the Danish Fetal Medicine Database at Aarhus University Hospital, from 2010 to 2014. Social security numbers were linked to the microbiological database with the registration of 17,233 urine cultures in 8,807 women. Bacteriuria was defined as 1×105 CFU/ml, with a maximum of two urinary pathogens. Streptococcus agalactiae (GBS) was included with 1×104 CFU/ml. Data are presented as numbers and proportions in percent. Logistic regression on predictors are presented as crude and adjusted odds ratios (ORc/ORa) with 95% confidence intervals (CIs). Results. 42% had a urine sample culture test at the hospital—the majority only once during pregnancy. 96% of all urine culture tests were negative. The bacteriuria incidence was 5.6%. The most frequent uropathogenic bacteria isolated were Escherichia coli (49%), GBS (29%), and Enterococci (10%). We identified subgroups of women with increased likelihood of bacteriuria during pregnancy: age<25 years, ORa 1.60 (CI 1.26 to 2.02, p<0.001); age>34 years, ORa 1.28 (CI 1.01 to 1.61, p=0.040); Afro-Caribbean origin, ORa 1.872 (CI 1.13 to 3.07, p=0.014); Asian origin, ORa 2.07 (CI 1.29 to 3.32, p=0.002); and mixed ethnicity ORa 2.34 (CI 1.23 to 4.46, p=0.010). Women delivering preterm were more likely to have an episode of bacteriuria during pregnancy OR 2.05 (CI 1.36 to 3.09, p=0.001). Conclusions. 96% of urine culture tests in pregnant women are negative. Optimized urine sampling may change this. Escherichia coli and GBS are predominant uropathogens. Younger and elder women, certain ethnical groups, and women delivering preterm seem more likely to have bacteriuria during pregnancy.

scholarly journals Prevalence and bacterial susceptibility of hospital acquired urinary tract infection

2003 ◽  
Vol 18 (suppl 5) ◽  
pp. 36-38 ◽  
Author(s):  
José Anastácio Dias Neto ◽  
Leonardo Dias Magalhães da Silva ◽  
Antonio Carlos Pereira Martins ◽  
Ricardo Brianezi Tiraboschi ◽  
André Luis Alonso Domingos ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antibiotic Susceptibility ◽  
Urine Culture ◽  
Generation Cephalosporin ◽  
University Hospital ◽  
Third Generation ◽  
Bacterial Strains ◽  
Hospital Acquired

PURPOSE: Urinary tract infection is the most common nosocomially acquired infection. It is important to know the etiology and antibiotic susceptibility infectious agents to guide the initial empirical treatment. OBJECTIVE: To determine the prevalence of bacterial strains and their antibiotic susceptibility in nosocomially acquired urinary tract infection in a university hospital between January and June 2003. METHODS: We analyzed the data of 188 patients with positive urine culture (= 10(5) colony-forming units/mL) following a period of 48 hours after admission. RESULTS: Half of patients were male. Mean age was 50.26 ± 22.7 (SD), range 3 months to 88 years. Gram-negative bacteria were the agent in approximately 80% of cases. The most common pathogens were E. coli (26%), Klebsiella sp. (15%), P. aeruginosa (15%) and Enterococcus sp. (11%). The overall bacteria susceptibility showed that the pathogens were more sensible to imipenem (83%), second or third generation cephalosporin and aminoglycosides; and were highly resistant to ampicillin (27%) and cefalothin (30%). It is important to note the low susceptibility to ciprofloxacin (42%) and norfloxacin (43%). CONCLUSION: This study suggests that if one can not wait the results of urine culture, the best choices to begin empiric treatment are imipenem, second or third generation cephalosporin and aminoglycosides. Cefalothin and ampicillin are quite ineffective to treat these infections.

An Ultrastructural Study of the Proliferation Patterns of the Reserve Cells of the Human Cervix Uteri

1972 ◽  
Vol 30 ◽  
pp. 80-81
Author(s):  
Takanori Sohda ◽  
Hiroshi Saito ◽  
Goro Asano ◽  
Katsunari Fukushi ◽  
Katsuya Suzuki ◽  
...  
Keyword(s):  
Ultrastructural Study ◽  
Age Groups ◽  
Point Of View ◽  
University Hospital ◽  
Cervix Uteri ◽  
Morphological Aspect ◽  
Functional Aspect ◽  
Pathological Conditions ◽  
Human Cervix ◽  
Morphological Patterns

Recently, the functional aspect as well as morphological aspect of the reserve cells in the cervix uteri drew much attention in view of the carcinogenesis in squamocolumunar junction. In this communication, the authors elucidate the ultrastructural features of the reserve cells in patients of various age groups visiting our university hospital and affiliated hospital.From conventional light microscopic point of view, the reserve cells tend to be pronounced in various pathological conditions, such as the persisting inflammation, proliferative disorders and irritation of hormones. The morphological patterns of the reserve cells from various stage and degree of irritation were observed.

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