scholarly journals Biometry of fruit, germination and morphogenesis in vitro of citrus after asepsis of seeds in different concentrations of sodium hypochlorite

2020 ◽  
Vol 13 (3) ◽  
pp. 14
Author(s):  
D. Santos ◽  
W. J. Pereira ◽  
D. S. Miranda ◽  
J. L. C. Souza ◽  
L. A. Borges ◽  
...  
Keyword(s):  
Sodium Hypochlorite ◽  
In Vitro Cultivation ◽  
Sodium Hypochlorite Solution ◽  
Citrus Aurantium ◽  
Federal Institute ◽  
Growth Room ◽  
Morphogenesis In Vitro ◽  
Morphological Processes

In tissue culture it is necessary to use seeds of good quality and free of pathogens in order to avoid damages in the work to be carried out in the laboratory. Therefore, strict cleaning of the seeds intended for in vitro cultivation is essential. Thus, the objective of this study was to evaluate fruit biometry, germination and morphogenesis of 'Cravo' (Citrus limonia Osbeck) and Persia Lima (Citrus aurantium) seeds in vitro submitted to different concentrations of NaClO for asepsis. Fruits of 'Cravo' lemon and Lima of Persia. Subsequently, they were transported to the Biotechnology Laboratory of the Goiano Federal Institute Campus Urutaí-GO, where they performed the biometric characterization of the fruits. The seeds of these fruits were submitted to treatment with sodium hypochlorite solution in different concentrations: 0,0; 1.0; 1.5; 2.0 and 2.5% for 10 minutes, constituting then five treatments for each variety and placed in an acclimatized growth room to analyze the results. The variables evaluated were: biometry of fruits and seedlings; oxidation indexes; germination; contamination; and types of contamination. NaClO doses were compared from 95% confidence intervals. The analyzes were performed using the glm () function of software R version 3.4.1. It was verified that the aseptic treatment with NaClO for lime and lemon was promising for the morphological processes of germination and development of seedlings, as well as for the control of contaminating agents in vitro.

Cloning and Molecular Characterization of a Multicopy, Linear Plasmid-Carried, Repeat Motif-Containing Gene from Borrelia turicatae, a Causative Agent of Relapsing Fever

1998 ◽  
Vol 180 (18) ◽  
pp. 4974-4981 ◽  
Author(s):  
Jason A. Carlyon ◽  
Richard T. Marconi
Keyword(s):  
Transcriptional Start Site ◽  
Gene Families ◽  
In Vitro Cultivation ◽  
Relapsing Fever ◽  
Related Sequence ◽  
Site Analysis ◽  
Low Levels ◽  
Ribosomal Binding Site

ABSTRACT Borrelia turicatae is one of several spirochete species that can cause relapsing fever. Here, we describe the identification and characterization of a gene from B. turicatae and other relapsing-fever spirochetes that exhibits homology with therep + and ORF-E gene families of the Lyme disease spirochetes. This gene, which we have designatedrepA, encodes a putative protein of 30.2 kDa with an isoelectric point of 4.69. The central region of RepA harbors a series of amino acid repeat motifs which exhibit homology with casein kinase 2 phosphorylation sites. Through Southern hybridization analyses, we demonstrate that repA (or a closely related sequence) is multicopy in the relapsing-fever spirochetes and is carried on variably sized linear plasmids in bothBorrelia parkeri and B. turicatae. Transcriptional analyses demonstrate that repA is expressed, albeit at low levels, during in vitro cultivation ofB. turicatae. Transcriptional start site analysis revealed that repA is preceded by a consensus ribosomal binding site and an appropriately spaced promoter element. The sequence conservation, unique features, and multicopy status of repAand its homologs suggest that RepA may play an important genus-wide role in the biology of the Borrelia.

scholarly journals Seed disinfestation methods for in vitro cultivation of epiphyte orchids from Southern Brazil

2006 ◽  
Vol 24 (2) ◽  
pp. 217-220 ◽  
Author(s):  
Veronica M Alvarez-Pardo ◽  
Alfredo Gui Ferreira ◽  
Valter F Nunes
Keyword(s):  
Sodium Hypochlorite ◽  
Active Chlorine ◽  
In Vitro Cultivation ◽  
Southern Brazil ◽  
Efficient Procedure

Disinfestation of orchid seeds is necessary when the germination is performed in vitro. In such case, cultures are supplemented by a source of sugar and salts in order to obtain energy and other requirements. However, the presence of sugar and salts easily allows the development of unwanted microorganisms. To avoid such phenomenon, an efficient procedure must be done as a pretreatment of the seeds with liquid or gaseous substances. In this study with several Brazilian orchids, the seeds were disinfested with sodium hypochlorite solutions containing 0.4% or 0.8% active chlorine for five minutes and sodium hypochlorite or formaldehyde fumes up to two hours, this procedure being efficient for such purpose and making it possible to be used routinely.

In vitro cultivation of Hematodinium sp. isolated from Atlantic snow crab, Chionoecetes opilio: partial characterization of late developmental stages

Parasitology ◽  
2014 ◽  
Vol 142 (04) ◽  
pp. 598-611 ◽  
Author(s):  
PETER H. GAUDET ◽  
RICHARD J. CAWTHORN ◽  
MELANIE A. BUOTE ◽  
J. FRANK MORADO ◽  
GLENDA M. WRIGHT ◽  
...  
Keyword(s):  
Developmental Stages ◽  
Partial Characterization ◽  
Snow Crab ◽  
In Vitro Cultivation ◽  
Chionoecetes Opilio

scholarly journals Antimicrobial Efficacy of Herbal Root Canal Irrigants and 3% Sodium Hypochlorite against Enterococcus faecalis: An In-vitro Study

2021 ◽  
pp. 74-78
Author(s):  
Shafie Ahamed ◽  
Vignesh Guptha Raju ◽  
Madhuram Krishnamurthy ◽  
V. Naveen Kumar ◽  
K. E. Selvendran
Keyword(s):  
Sodium Hypochlorite ◽  
Leaf Extract ◽  
Dental Treatment ◽  
Nigella Sativa ◽  
Dilution Method ◽  
Sodium Hypochlorite Solution ◽  
Root Canal Irrigants ◽  
Neem Leaf Extract ◽  
Aqueous Neem Leaf Extract

Nigella sativa (kalonji) has been used since ancient times as a nutritional supplement and for treating various infections and chronic ailments. As pathogens become resistant to most drugs, kalonji can be used as an alternative compound in modern medicines. The use of herbal extracts as endodontic irrigants might be beneficial as a part of a growing trend to seek natural remedies for dental treatment. Aim:  To compare the antibacterial potency of Aqueous Nigella sativa extract, Aqueous Neem leaf extract, and 3% Sodium Hypochlorite. Materials and Methods: Test solutions were tested against E. faecalis (ATCC 29212) to check for their Minimum Inhibitory Concentration (MIC) by double dilution method and Kill time to measure their antimicrobial potency to be used as an intracanal irrigant. Results: The study's limitations show that Aqueous Nigella sativa extract has a better antimicrobial effect than Aqueous Neem leaf extract and 3% Sodium Hypochlorite solution against E. faecalis.

scholarly journals Formation and survival of in vitro protocormos of Cerrado Orchid of Goiás

2020 ◽  
Vol 13 (6) ◽  
pp. 1
Author(s):  
D. C. S. Lemos ◽  
G. R. Sousa ◽  
A. D. V. Souza ◽  
J. B. A. França ◽  
M. R. Rosa ◽  
...  
Keyword(s):  
Bacterial Contamination ◽  
Sodium Hypochlorite Solution ◽  
Ms Medium ◽  
In Vitro Development ◽  
Federal Institute ◽  
In The Wild ◽  
Vitro Development ◽  
Mature Seeds ◽  
Pot Plants

The Cerrado has wide plant diversity, including orchids. These plants need symbiosis with fungi for their germination to occur in the wild. In vitro or asbiotic cultivation is an alternative for the production of seedlings of this species. This diversity of orchids makes it much appreciated as pot plants, landscaping, with high commercial value. The objective of this work was to carry out the study for the development of a protocol for the germination and in vitro development of orchids. The experiment was carried out at the LABIOTEC Biotechnology Laboratory of the Federal Institute Goiano Campus Urutaí-GO. Catasetum sp native capsules from Goiás State, containing mature seeds were collected and disinfected in 50% sodium hypochlorite solution for 30 minutes and in laminar flow were washed four times in distilled and autoclaved water. They were then seeded in vitro in 258 mL glass vials containing 30 mL of MS medium enriched with vitamins and 0.0 Tryptone; 1.0; 2.0 and 3.0 g L-1. The pH of the medium was adjusted to 5.2 ± 0.1. The average time for germination and development of the protocorms varied from 3 to 7 days after sowing for the species under study. The dosage of 2.0 g L-1 is efficient for the development and maintenance of in vitro Catasetum protocorms up to 333 days. There was no development of shoot of Catasetum seedlings in any of the evaluated treatments. It was observed that the 3.0 g L-1 Tryptone dosage presented higher rates of fungal and bacterial contamination and oxidation

Evaluation of topical therapies for the treatment of dermatophyte-infected hairs from dogs and cats

1995 ◽  
Vol 31 (3) ◽  
pp. 250-253 ◽  
Author(s):  
N White-Weithers ◽  
L Medleau
Keyword(s):  
Antifungal Activity ◽  
Sodium Hypochlorite ◽  
Povidone Iodine ◽  
Fungal Growth ◽  
In Vitro Study ◽  
Test Period ◽  
Microsporum Canis ◽  
Sodium Hypochlorite Solution ◽  
Hypochlorite Solution

Seven commonly used, topical antifungal products (i.e., lime sulfur, chlorhexidine, captan, povidone-iodine, sodium hypochlorite, and enilconazole solutions, and ketoconazole shampoo) were evaluated for their antifungal activity on Microsporum canis-infected hairs from dogs and cats in an in vitro study. Hairs were soaked or shampooed in each product for five minutes twice a week for four weeks. Of the seven products used in this study, lime sulfur and enilconazole solutions were superior in inhibiting fungal growth; no growth occurred on fungal cultures after two treatments with either product. Chlorhexidine and povidone iodine solutions were effective after four treatments, and sodium hypochlorite solution and ketoconazole shampoo inhibited fungal growth after eight treatments. Captan did not inhibit fungal growth during the test period.

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