Evaluation of topical therapies for the treatment of dermatophyte-infected hairs from dogs and cats

1995 ◽  
Vol 31 (3) ◽  
pp. 250-253 ◽  
Author(s):  
N White-Weithers ◽  
L Medleau
Keyword(s):  
Antifungal Activity ◽  
Sodium Hypochlorite ◽  
Povidone Iodine ◽  
Fungal Growth ◽  
In Vitro Study ◽  
Test Period ◽  
Microsporum Canis ◽  
Sodium Hypochlorite Solution ◽  
Hypochlorite Solution

Seven commonly used, topical antifungal products (i.e., lime sulfur, chlorhexidine, captan, povidone-iodine, sodium hypochlorite, and enilconazole solutions, and ketoconazole shampoo) were evaluated for their antifungal activity on Microsporum canis-infected hairs from dogs and cats in an in vitro study. Hairs were soaked or shampooed in each product for five minutes twice a week for four weeks. Of the seven products used in this study, lime sulfur and enilconazole solutions were superior in inhibiting fungal growth; no growth occurred on fungal cultures after two treatments with either product. Chlorhexidine and povidone iodine solutions were effective after four treatments, and sodium hypochlorite solution and ketoconazole shampoo inhibited fungal growth after eight treatments. Captan did not inhibit fungal growth during the test period.

scholarly journals The Antibacterial Efficacy of Biopure MTAD in Root Canal Contaminated with Enterococcus faecalis

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Blerim Kamberi ◽  
Donika Bajrami ◽  
Miranda Stavileci ◽  
Shuhreta Omeragiq ◽  
Fatmir Dragidella ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Sodium Hypochlorite Solution ◽  
Antibacterial Efficacy ◽  
Human Teeth ◽  
Exact Test ◽  
Hypochlorite Solution ◽  
Root Canals ◽  
Significant Difference ◽  
Level Of Significance

Aim. The purpose of this in vitro study was to assess the antimicrobial efficacy of Biopure MTAD against E. faecalis in contaminated root canals. Materials and Methods. Forty-two single rooted extracted human teeth were inoculated with E. faecalis and incubated for four weeks. The samples were divided in two control and five experimental groups irrigated with 1.5% sodium hypochlorite solution (NaOCl); 3% NaOCl; BioPure MTAD; 1.5% NaOCl/17% EDTA; or 3% NaOCl/17% EDTA. After a one-week incubation, complete disinfection was confirmed by the absence of turbidity in the incubation media. Dentin shavings were taken from samples with no turbidity to verify whether E. faecalis was present in dentin tubules. Results were analyzed statistically using Fisher's exact test, with the level of significance set at . Results. Statistical analysis of the data obtained at Day 7 and after dentin shaving analysis showed that BioPure MTAD had significantly greater antibacterial activity than 1.5% NaOCl, 1.5% NaOCl/17% EDTA and 3% NaOCl/17% EDTA. No significant difference was detected between MTAD and 3% NaOCl. Conclusions. These findings suggest that BioPure MTAD possesses superior bactericidal activity compared with NaOCl and EDTA against E. faecalis.

Comparison of the Efficacy of a Hydrogen Peroxide Dry-Mist Disinfection System and Sodium Hypochlorite Solution for Eradication of Clostridium difficile Spores

2009 ◽  
Vol 30 (6) ◽  
pp. 507-514 ◽  
Author(s):  
F. Barbut ◽  
D. Menuet ◽  
M. Verachten ◽  
E. Girou
Keyword(s):  
Hydrogen Peroxide ◽  
Sodium Hypochlorite ◽  
Environmental Contamination ◽  
Bacterial Count ◽  
Sodium Hypochlorite Solution ◽  
Hypochlorite Solution ◽  
Colony Forming Units ◽  
In Situ Experiments

Objective.To compare a hydrogen peroxide dry-mist system and a 0.5% hypochlorite solution with respect to their ability to disinfect Clostridium difficile-contaminated surfaces in vitro and in situ.Design.Prospective, randomized, before-after trial.Setting.Two French hospitals affected by C. difficile.Intervention.In situ efficacy of disinfectants was assessed in rooms that had housed patients with C. difficile infection. A prospective study was performed at 2 hospitals that involved randomization of disinfection processes. When a patient with C. difficile infection was discharged, environmental contamination in the patient's room was evaluated before and after disinfection. Environmental surfaces were sampled for C. difficile by use of moistened swabs; swab samples were cultured on selective plates and in broth. Both disinfectants were tested in vitro with a spore-carrier test; in this test, 2 types of material, vinyl polychloride (representative of the room's floor) and laminate (representative of the room's furniture), were experimentally contaminated with spores from 3 C. difficile strains, including the epidemic clone ribotype 027-North American pulsed-field gel electrophoresis type 1.Results.There were 748 surface samples collected (360 from rooms treated with hydrogen peroxide and 388 from rooms treated with hypochlorite). Before disinfection, 46 (24%) of 194 samples obtained in the rooms randomized to hypochlorite treatment and 34 (19%) of 180 samples obtained in the rooms randomized to hydrogen peroxide treatment showed environmental contamination. After disinfection, 23 (12%) of 194 samples from hypochlorite-treated rooms and 4 (2%) of 180 samples from hydrogen peroxide treated rooms showed environmental contamination, a decrease in contamination of 50% after hypochlorite decontamination and 91% after hydrogen peroxide decontamination (P < .005). The in vitro activity of 0.5% hypochlorite was time dependent. The mean (±SD) reduction in initial log10 bacterial count was 4.32 ± 0.35 log10 colony-forming units after 10 minutes of exposure to hypochlorite and 4.18 ± 0.8 logl0 colony-forming units after 1 cycle of hydrogen peroxide decontamination.Conclusion.In situ experiments indicate that the hydrogen peroxide dry-mist disinfection system is significantly more effective than 0.5% sodium hypochlorite solution at eradicating С difficile spores and might represent a new alternative for disinfecting the rooms of patients with C. difficile infection.

scholarly journals Comparing the Antifungal Effect of Sodium Hypochlorite Gel versus Different Types of Root Canal Medicaments at Different Time Intervals Using the Agar Diffusion Test: An In Vitro Study

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Mohamed El Sayed ◽  
Nikta Ghanerad ◽  
Zeinab Shabanpour ◽  
Mahin Shabanpoor ◽  
Fatemeh Rahimi
Keyword(s):  
Antifungal Activity ◽  
Calcium Hydroxide ◽  
Sodium Hypochlorite ◽  
In Vitro Study ◽  
Significant Inhibition ◽  
Time Interval ◽  
Time Intervals ◽  
Significance Level ◽  
Agar Diffusion Test

Aims. The purpose of this study was to compare the antifungal activity of sodium hypochlorite gel to those of four intracanal medicaments at various time intervals. Materials and Methods. The agar well diffusion technique was utilized to test the antifungal activity of the following medicaments against Candida albinans (C. albicans): sodium hypochlorite gel, chlorhexidine gel, calcium hydroxide paste, Ledermix, and Diapex Plus. The inhibition zone related to each medicine was measured in millimeter after 24, 48, and 72 hours of incubation at 37°C. The data were analyzed using one-way ANOVA and Games–Howell tests, at a significance level of P < 0.05 . Results. Except for Ledermix and Diapex Plus, which had no antifungal action, all of the medicaments showed varied inhibitory zones for C. albicans. At all periods, the NaOCl gel had the most significant inhibition zones, followed by the CHX gel and calcium hydroxide. At all time intervals, the NaOCl gel demonstrated comparable antifungal efficacy. When compared to a 24-hour time interval, the CHX gel showed an increased antifungal activity at the 48-hour and 72-hour intervals. Calcium hydroxide, on the other hand, showed a decrease in its antifungal activity at the 72-hour interval. Conclusion. The antifungal activity of sodium hypochlorite gel was the highest among the investigated medicaments. Chlorhexidine gel's antifungal activity improved over time, whereas calcium hydroxide's antifungal activity declined. Diapex Plus and Ledermix did not have any antifungal properties.

scholarly journals Evaluation of ph stability, concentration and antimicrobial activity in commercial brands of sodium hypochlorite solution

2021 ◽  
Vol 10 (3) ◽  
pp. e54910313658
Author(s):  
Bianca Maria Beserra Costa ◽  
Maria Katharina Peixoto da Silva ◽  
Sandra Maria Alves Sayão Maia ◽  
Paulo Maurício Reis de Melo Júnior ◽  
Natália Gomes de Oliveira ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Sodium Hypochlorite ◽  
In Vitro Study ◽  
Chlorine Content ◽  
Active Chlorine ◽  
Diffusion Method ◽  
Sodium Hypochlorite Solution ◽  
Disk Diffusion Method ◽  
Storage Location

The present work refers to an in vitro study of different commercial brands of sodium hypochlorite (manipulated, Soda Chlorine and Brilux) being evaluated by means of pH, antimicrobial action and concentration, at time intervals of 0, 7 and 14 days, in a refrigerated environment and at room temperature, and with receiving and absence of light. The antimicrobial assay of the solutions was performed by the disk diffusion method. Enterococcus faecalis was grown on Tryptic Soil Agar (TSA) (Merk) and incubated at ± 35ºC for 24 hours. The active chlorine content was verified by iodometric titration, and the pH, by pH measuring tape. The results suggest that there was no statistically significant relevance in the results of the active chlorine content. The pH became higher with time, and the antimicrobial activity was altered according to the storage location and time.

scholarly journals MICROPROPAGATION OF GLADIOLUS × HYBRIDUS HORT. ‘ПЕРМСКИЙ СУВЕНИР ’, WHICH WAS BRED IN BOTANICAL GARDEN OF PSU

2020 ◽  
pp. 97-102
Author(s):  
N. L. Shibanova ◽  
◽  
M. A. Chertkova ◽  
T. D. Chemarova ◽  
◽  
...  
Keyword(s):  
Survival Rate ◽  
Sodium Hypochlorite ◽  
Botanical Garden ◽  
Sodium Hypochlorite Solution ◽  
Hypochlorite Solution ◽  
Sterile Culture ◽  
Murashige And Skoog Medium ◽  
Gladiolus Hybridus

The article presents data on micropropagation of new Gladiolus × hybridus cultivar ‘Пермский Сувенир’. It is found that for the sterilization of corms and cormels better use the 7% sodium hypochlorite solution (15 min or 20-25 min) and then 96% ethanol (30 sec or 60 sec). Sterile culture was more than 56% for corms and 76% for cormels. At the micropropagation good results were obtained when vitamins (0.1 mg/l thiamine, 0.5 mg/l pyridoxine, 0.5 mg/l nicotinic acid) and β-indolylacetic acid (0.1-1 mg/l) were added to the Murashige and Skoog medium. The breeding rate for cormels was 2-3, for corm fragments was 2–4. The survival rate of plants transferred from in vitro to in vivo conditions was 70%. The diameter of the obtained juvenile corms from in vitro culture and the number of cormels formed on them did not have statistically significant differences from those planted in vivo (t = [1.16; 1.22] <t05 = 1.96).

An In Vitro Analysis of Sodium Hypochlorite Decontamination for the Reuse of Implant Healing Abutments

2020 ◽  
Author(s):  
Ahmad Almehmadi
Keyword(s):  
Sodium Hypochlorite ◽  
Bacterial Culture ◽  
Brain Heart Infusion ◽  
In Vitro Study ◽  
Vitro Study ◽  
Sem Images ◽  
Streptococcus Sanguis ◽  
Implant Dentistry ◽  
Vitro Analysis

Abstract The re-use of healing abutments (HAs) has become common practice in implant dentistry for economic concerns and the aim of this in-vitro study was to assess the effect of sodium hypochlorite (NaOCl) in decontamination of HAs. 122 HAs (Used and sterilized n=107; New n=15) were procured from 3 centers, of which 3 samples were discarded due to perforation in sterilization pouch.  For sterility assessment, the used HAs (n=80) were cultured in Brain Heart Infusion Broth (BHI) and Potato Dextrose Agar (PDA), bacterial isolates were identified in 7 samples. Also, 24 used HAs were stained with Phloxine B, photographed and compared to new HAs (n=5). Scanning electron microscope (SEM) assessed the differences between the two sets of HAs, following which the 7 contaminated HAs along with 24 used HAs from staining experiment (Total=31) were subsequently treated with sodium hypochlorite (NaOCl) and SEM images were observed. About 8.75% of HAs tested positive in bacterial culture; Streptococcus sanguis, Dermabacter hominis, Staphylococcus haemolyticus, and Aspergillus species were isolated. Phloxine B staining was positive for used and sterilized HAs when compared to controls. The SEM images revealed deposits in the used HAs and although treatment with NaOCl eliminated the contamination of cultured HAs, the SEM showed visible debris in the HA thread region. This in-vitro study concluded that SEM images showed debris in used HAs at screw-hole and thread regions even though they tested negative in bacterial culture. The treatment with NaOCl of used HAs showed no bacterial contamination but the debris was observed in SEM images. Future studies on the chemical composition, biological implications, and clinical influence is warranted before considering the reuse of HAs.

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