Enzymatic activity of strains of bacteria Bacillus subtilis isolated from frozen soils

2020 ◽  
Vol 1 (1) ◽  
pp. 73-79
Author(s):  
M.P. Scriabina ◽  
◽  
A.M. Stepanova ◽  
N.P. Tarabukina ◽  
M.P. Neustroev ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Enzymatic Activity ◽  
Frozen Soils

PRODUCTION OF CYTASES ACTIVE ON BARLEY GUM BY BACTERIA OF THE GENUS BACILLUS

1953 ◽  
Vol 31 (1) ◽  
pp. 28-32 ◽  
Author(s):  
A. C. Blackwood
Keyword(s):  
Bacillus Subtilis ◽  
Enzymatic Activity ◽  
Nitrogen Source ◽  
Freeze Drying ◽  
Shake Flask ◽  
Sole Nitrogen Source ◽  
Genus Bacillus ◽  
Original Activity ◽  
Bacterial Cultures ◽  
Bacillus Genus

One hundred and fourteen bacterial cultures representing most of the species in the Bacillus genus were tested for the production of extracellular barley gum cytase. Assays were made on shake-flask cultures grown on a medium containing glucose and yeast extract. Although all the organisms had some enzymatic activity, certain strains of Bacillus subtilis gave the best yields of cytase. On a medium with asparagine as the sole nitrogen source even higher yields were obtained. The crude cytase preparations were stable and after freeze-drying most of the original activity remained.

scholarly journals The Importance of the Interaction of CheD with CheC and the Chemoreceptors Compared to Its Enzymatic Activity during Chemotaxis in Bacillus subtilis

PLoS ONE ◽  
2012 ◽  
Vol 7 (12) ◽  
pp. e50689 ◽  
Author(s):  
Wei Yuan ◽  
George D. Glekas ◽  
George M. Allen ◽  
Hanna E. Walukiewicz ◽  
Christopher V. Rao ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Enzymatic Activity

Immobilization of Bacillus subtilis lipase on a Cu-BTC based hierarchically porous metal–organic framework material: a biocatalyst for esterification

2016 ◽  
Vol 45 (16) ◽  
pp. 6998-7003 ◽  
Author(s):  
Yu Cao ◽  
Zhuofu Wu ◽  
Tao Wang ◽  
Yu Xiao ◽  
Qisheng Huo ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Enzymatic Activity ◽  
Metal Organic Framework ◽  
Porous Metal ◽  
Esterification Reaction ◽  
Hierarchically Porous ◽  
Metal Organic ◽  
First Time ◽  
High Enzymatic Activity ◽  
Organic Framework

Bacillus subtilislipase (BSL2) has been successfully immobilized into a Cu-BTC based hierarchically porous MOF material for the first time. The immobilized BSL2 presents high enzymatic activity and perfect reusability during the esterification reaction.

scholarly journals Biodegradation Capacity and Activity Enzymatic of Bacillus subtilis against Low-Density Polyethylene

2021 ◽  
pp. 9-22
Author(s):  
Ivana Ortega Rojas ◽  
Adriana Rodríguez Pérez ◽  
Juan Fernando Cárdenas González ◽  
Víctor Manuel Martínez Juárez ◽  
Erika Enriquez Domínguez ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Enzymatic Activity ◽  
Laccase Activity ◽  
Dry Weight ◽  
Extracellular Protein ◽  
Low Density Polyethylene ◽  
Low Density ◽  
Extracellular Laccase ◽  
Bacterium Bacillus ◽  
Static Conditions

Aims: The objective of this work was to determine the degradation capacity of low-density polyethylene by the bacterium Bacillus subtilis and analyze the production of extracellular laccase activity. Methodology: The experiments was realized in 50 mL of culture medium, added with a fragment of known dry weight (1 cm2 colorless polyethylene bag squares), and were incubated at 28°C, pH 6.5, for 6 months under static conditions, determining the growth of the bacterium by dry weight (68, 75, and 91 mg), the production of extracellular protein (271, 234, and 326.1 mg/mL), and the degradation of the substrate by dry biodegraded (8.57%, 5.88%, and 11.76%). Results: The production of extracellular laccase enzyme was analyzed in presence of polyethylene, finding an enzymatic activity of laccase of 2.06, 1.49, and 2,03 U/mL, while in the control without substrate, no enzymatic activity was observed, which suggests that this enzyme may participate in the degradation of polyethylene. In addition, some characteristics of the extracellular enzymatic activities were analyzed, such as stability at 4oC and 28oC, optimal pH and temperature, the effect of protein and substrate concentration. Conclusion: The extracellular protein production and dry weight of the bacterium are higher in the presence of low-density polyethylene. The laccase activity is very stable at 4oC and 28oC, the most effective pH and temperature, were 4.5 and 28oC, and present an incubation time of 5 minutes, and this data suggest that this enzymatic activitiy may participate in the degradation of low density polyethylene.

scholarly journals Improvement of the nutritional value and growth of rotifer (Brachionus plicatilis) by different enrichment period with Bacillus sp.

2011 ◽  
Vol 10 (1) ◽  
pp. 67
Author(s):  
Sutia Budi ◽  
. Zainuddin ◽  
Siti Aslamsyah
Keyword(s):  
Bacillus Subtilis ◽  
Enzymatic Activity ◽  
Nutritional Value ◽  
Brachionus Plicatilis ◽  
Bacillus Sp ◽  
Fiber Volume ◽  
Culture Experiment ◽  
Growth Optimum ◽  
Rotifer Brachionus Plicatilis

<p><em>Brachionus</em><em> plicatilis</em> is a widely used as natural feed in hatchery. Characteristics of rotifers as biocapsule can improve the quality of rotifers in a practical way. <em>Bacillus</em><em> </em>sp. is capable of improving the nutrition rotifers to produce vitamins, food detoxication or through enzymatic activity such as protease, lipase, and amylase. This research aimed at observing the influence of the enrichment length of <em>Bacillus </em>sp. on the quality of nutrition and growth of rotifers. The research container was a <em>polyearbonate</em> vessel of one ton volume which contained <em>Nannochloropsis</em> sp. with the density of 10<sup>5</sup> cell/mL, it was then scattered with rotifer with the density of 1,000 ind./mL. The types of bacteria used were <em>Bacillus subtilis</em>, <em>B. pumilus</em> and <em>B. licheformis</em> with the density of 2x10<sup>10</sup> cfu/g. The experiments tried with different enrichment length were A = 0 hour, B = 5 hours, C = 10 hours and D = 10 hours with triplicate. As the comparing data, rotifer culture experiment was carried out with <em>Nannochlorophsis </em>sp.as the control. The number of containers were 24 which consists of 12 for threatment and 12 pieces as control. Variables measured were proximate to each treatment and the growth of rotifers. The result of the research revealed the enrichment length of rotifers with <em>Bacillus</em> sp. has significantly (P<0,05) towards increased protein, crude fiber, ash, BETN, and growth. Optimum enrichment time was obtained at 5 hours.</p> <p>Key words: rotifers, <em>Bacillus</em>, enrichment period, proximate</p> <p> </p> <p>ABSTRAK</p> <p><em>Brachionus plicatilis</em> merupakan pakan alami yang banyak digunakan dalam pembenihan. Karakteristik rotifer sebagai biokapsul secara praktis dapat meningkatkan kualitas rotifer. <em>Bacillus </em>sp<em>.</em> mampu memperbaiki nutrisi rotifer dengan memproduksi vitamin, detoksikasi pangan maupun melalui aktivitas enzimatis seperti <em>protease</em>, <em>lipase</em>, dan <em>amilase</em>. Penelitian ini bertujuan untuk melihat pengaruh pengkayaan <em>Bacillus </em>sp<em>.</em> terhadap kualitas nutrisi dan pertumbuhan rotifer. Wadah penelitian menggunakan bak fiber volume 1 ton yang berisikan <em>Nannochloropsis</em> sp<em>.</em> dengan kepadatan 10<sup>5</sup> cell/mL, kemudian ditebari rotifer dengan kepadatan 1.000 ind./mL. Jenis bakteri yang digunakan berupa <em>Bacillus</em> <em>subtilis</em>, <em>B. Pumilus </em>dan<em> B. Licheformis </em>dengan kepadatan 2x10<sup>10</sup> cfu/g. Perlakuan yang diujikan adalah lama pengkayaan probiotik <em>Bacillus </em>sp<em>.</em> yang berbeda, yaitu perlakuan A (0 jam),  perlakuan B (5 Jam), perlakuan C (10 jam) dan perlakuan D (15 jam). Sebagai data pembanding dilakukan percobaan kultur rotifer dengan <em>Nannochloropsis</em> sp<em>.</em> sebagai kontrol. Jumlah wadah penelitian yang dipergunakan sebanyak 24 buah yang terdiri atas 12 buah untuk perlakuan dan 12 buah sebagai kontrol. Peubah yang diukur adalah proksimat pada masing-masing perlakuan dan pertumbuhan rotifer.  Hasil penelitian menunjukkan bahwa perlakuan lama pengkayaan rotifer dengan <em>Bacillus</em> sp<em>.</em> meningkatkan (P<0,05) terhadap kadar protein, serat kasar, abu, BETN, dan pertumbuhan rotifer. Lama pengkayaan optimum diperoleh pada 5 jam.</p> <p>Kata kunci: rotifer, <em>Bacillus</em>, lama pengkayaan, proksimat</p>

ENZYMATIC ACTIVITY OF XYLANASES AND CELLULASES OF PROBIOTIC STRAINS BACILLUS SUBTILIS

2019 ◽  
Vol 3 ◽  
pp. 19-24
Author(s):  
Rish.S. Mukhammadiev ◽  
◽  
Rin.S. Mukhammadiev ◽  
L.R. Valiullin ◽  
V.V. Biryulya ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Enzymatic Activity ◽  
Probiotic Strains

scholarly journals Functional Roles of the Conserved Glu304 Loop of Bacillus subtilis Glutamine Synthetase

2010 ◽  
Vol 192 (19) ◽  
pp. 5018-5025 ◽  
Author(s):  
Lewis V. Wray ◽  
Susan H. Fisher
Keyword(s):  
Bacillus Subtilis ◽  
Glutamine Synthetase ◽  
Enzymatic Activity ◽  
Feedback Inhibition ◽  
Constitutive Expression ◽  
Methionine Sulfoximine ◽  
Wild Type ◽  
Functional Roles ◽  
Glutamate Binding ◽  
High Level

ABSTRACT The enzymatic activity of Bacillus subtilis glutamine synthetase (GS), which catalyzes the synthesis of glutamine from ammonium and glutamate, is regulated by glutamine feedback inhibition. The feedback-inhibited form of B. subtilis GS regulates the DNA-binding activities of the TnrA and GlnR nitrogen transcriptional factors. Bacterial GS proteins contain a flexible seven-residue loop, the Glu304 flap, that closes over the glutamate entrance to the active site. Amino acid substitutions in Glu304 flap residues were examined for their effects on gene regulation, enzymatic activity, and feedback inhibition. Substitutions in five of the Glu304 loop residues resulted in constitutive expression of both TnrA- and GlnR-regulated genes, indicating that this flap is important for regulating the activity of these transcription factors. The residues in the highly conserved Glu304 flap appear to be optimized for glutamate binding because mutant enzymes with substitutions in five of the flap residues had increased glutamate Km values compared to that for wild-type GS. The E304A and E304D substitutions increased the ammonium Km values compared to that for wild-type GS and conferred high-level resistance to inhibition by glutamine, glycine, and methionine sulfoximine. A model for the role of the Glu304 residue in glutamine feedback inhibition is proposed.

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