EFEK FUNGISTATIS, FUNGISIDAL EKSTRAK KAYU MANIS TERHADAP CANDIDA ALBICANS DAN EFEK BAKTERISTATIS BAKTERISIDAL TERHADAP STAPHYLOCOCCUS AUREUS DARI DENTURE STOMATITIS

Cinnamon is one of the spices that has been used for a long time, even before 2100 BC as an essential material to mummify a king's body and also as addition for foods and drinks to increase the flavours. Cinnamon has three active components such as cinnamaldehyd, eugenol and linalool. Cinnamon has good fungicidal and fungistatic effect against Candida albicans and bacteristatic, bactericidal effect against Staphylococcus aureus. Cinnamon can be used to decrease the amount of Candida albicans and Staphylococcus aureus colonies, so the researcher is interested to examine the fungistatic, fungicidal, bacteristatic dan bactericidal effect against Candida albicans and Staphylococcus aureus. The goal of this research is to determine the effect of different concentrations of cinnamon extract against Candida albicans and Staphylococcus aureus.In this research, the extract's concentrations that are used are 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,56%, 0,78%, 0,39%, 0,195% and also two controls which are formaldehyd and aquadest. The extract used in this research is obtained by extraction method. Dilution technique is used in the test with three times replication. This research is experimental laboratorium type with pretest-postest design. Kruskal-Wallis and Mann-Whitney test is used to analyze the data.The result show that concentration 0,78 % extract has shown fungistatic effect and concentration 25% has shown fungicidal effect against Candida albicans, and concentration 1,562% bacteristatic, concentration 50% bactericidal Staphylococcus aureus. From double comparison table, we can conclude that there are significant difference between each concentration's effect against Candida albicans and Staphylococcus aureus.The result shows that the increase of cinnamon extract's concentration will lessen the remaining colonies of Candida albicans.

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Pengaruh perendaman plat resin akrilik dalam ekstrak kayu manis (Cinnamomum burmanii) terhadap jumlah blastospora Candida Albicans

e-GIGI ◽

10.35790/eg.1.2.2013.3106 ◽

2013 ◽

Vol 1 (2) ◽

Author(s):

Christian Dama

Keyword(s):

Candida Albicans ◽

Acrylic Resin ◽

T Test ◽

Control Group ◽

Dilution Method ◽

Denture Stomatitis ◽

Cinnamon Extract ◽

Acrylic Plate ◽

Removable Denture ◽

Significant Difference

Resin akrilik sering digunakan sebagai bahan dari gigi tiruan, khususnya basis gigi tiruan. Adanya rongga-rongga mikro pada akrilik menjadi tempat perlekatan sisa-sisa makanan yang dapat meningkatkan jumlah mikroorganisme dalam rongga mulut, salah satunya ialah jamur Candida albicans. Pertumbuhan yang pesat dari jamur Candida albicans menjadi penyebab utama infeksi pada mukosa rongga mulut pemakai gigi tiruan lepasan akrilik, disebut denture stomatitis. Ekstrak kayu manis yang mengandung minyak atsiri, sinamaldehid, eugenol dan juga senyawa seperti flavonoid, Saponin, serta tanin memiliki efek antifungi. Tujuan penelitian ini untuk mengetahui pengaruh dari ekstrak kayu manis dan konsentrasi ekstrak kayu manis terhadap jumlah blastospora Candida albicans pada plat akrilik sehingga mampu mencegah dan menanggulangi penyakit denture stomatitis. Plat akrilik yang telah terkontaminasi dengan jamur Candida albicans direndam dalam ekstrak kayu manis dengan konsentrasi 20%, 30%, 40%, 50%, dan aquades sebagai kontrol. selanjutnya, plat akrilik di getarkan untuk menjatuhkan Candida albicans dalam tabung reaksi dan dihitung jumlah blastosporanya dengan metode pengenceran menggunakan cairan NaCl dan metode hitung langsung pada mikroskop. Hasil perhitungan statistik dengan uji Independent t-test diketahui terdapat perbedaan yang bermakna antara kelompok ekstrak kayu manis dan kelompok kontrol (p ≤ 0,05). Dari hasil penelitian dapat disimpulkan bahwa ekstrak kayu manis dapat memengaruhi pertumbuhan jumlah blastospora Candida albicans pada plat resin akrilik dan meningkat seiring dengan meningkatnya konsentrasi ekstrak kayu manis (cinnamomum burmanii) yang digunakan.Kata kunci: Resin akrilik, Candida albicans, ekstrak kayu manis.ABSTRACTAcrylic resin is often used as an ingredient of artificial teeth, denture base in particular. The existence of micro cavities in acrylic attachment to place leftover food that can increase the number of microorganisms in the oral cavity, one of which is Candida albicans. The rapid growth of Candida albicans is a main cause of infection in the oral mucosa acrylic removable denture wearers, called denture stomatitis. Cinnamon extract that contains essential oils, sinamaldehid, eugenol and also compounds such as flavonoids, saponins, and tannins have antifungal effects. The purpose of this study to determine the effect of cinnamon extract and cinnamon extract concentrations against Candida albicans blastospora number on acrylic plate so as to prevent and control disease denture stomatitis. Acrylic plate that has been contaminated with Candida albicans soaked in cinnamon extract with a concentration of 20%, 30%, 40%, 50%, and sterile water as a control. furthermore, acrylic plate vibrate to drop Candida albicans in a test tube and counted the number of blastospora by using saline dilution method and direct count method on the microscope. The results of calculations with the statistical test known Independent t-test significant difference between cinnamon extract group and the control group (p ≤ 0,05). From the results of this study concluded that cinnamon extract may affect the growth of Candida albicans blastospora on acrylic resin plate and increases with increasing concentration of the extract of cinnamon (Cinnamomum burmanii) were used.Keywords : Acrylic resin, Candida albicans, cinnamon extract.

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Prevalence of Candida albicans and Streptococcus aureus on maxillary obturators, maxillary defects and in saliva: A cross-sectional study

Brazilian Dental Science ◽

10.14295/bds.2017.v20i3.1366 ◽

2017 ◽

Vol 20 (3) ◽

Author(s):

Tamer Celakil ◽

Emrah Baca ◽

Emine Nursen Topcuoglu ◽

Bilge Gökcen Röhlig ◽

Gülümser Evlioglu ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Candida Albicans ◽

Oral Cavity ◽

Control Group ◽

Cross Sectional ◽

Denture Stomatitis ◽

Lining Material ◽

Renewal Period ◽

Maxillary Defects ◽

And Staphylococcus Aureus

Objective: This study investigated on the incidence of Candida albicans and Staphylococcus aureus adhesion onto maxillary obturator prostheses with soft lining materials placed by prosthodontists. Material and Methods: Between years 2013 and 2014, patients with maxillary defects due to maxillary tumor surgery and acquired obturator prostheses (n = 21, study group) were clinically evaluated and microbiological outcomes were compared with complete maxillary denture wearers (n = 21, control group). Data were collected on demographic features, histological type of cancer, presence of chemotherapy or radiotherapy, identification of soft lining materials used on obturator prostheses, cleansing methods practiced for obturator and maxillary complete prostheses. The presence of Candida albicans was determined on soft lining materials or polymethylmethacrylate surfaces and in the oral cavity, and Staphylococcus aureus in nasal mucosa and saliva. Data were analyzed using Kruskal Wallis, Fisher-Freeman-Halton tests (p < 0.05). Results: The highest amount of Candida albicans was found on surfaces of prostheses, in the saliva and on maxillary defects on the hard palate and on definitive soft lining material with 90%, 90%, and 70%, respectively. The highest amount of Staphylococcus aureus was observed in the saliva and nasal cavity in the definitive soft lining material group (50%). Conclusion: Short renewal period of lining materials may cause less Candida albicans colonisation and denture stomatitis in the oral cavity. Patients who have undergone maxillectomy were more prone to Staphylococcus aureus colonisation.KeywordsCandida albicans; Denture liners; Maxillary prosthesis; Palatal obturators; Staphylococcus aureus.

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Titanium Dioxide Addition to Heat Polymerized Acrylic Resin Denture Base Effect on Staphylococcus aureus and Candida albicans

Journal of Indonesian Dental Association ◽

10.32793/jida.v1i1.286 ◽

2018 ◽

Vol 1 (1) ◽

Author(s):

Dwi Tjahyaning Putranti ◽

Afrina Fadilla

Keyword(s):

Staphylococcus Aureus ◽

Titanium Dioxide ◽

Candida Albicans ◽

Tio2 Nanoparticles ◽

Base Material ◽

Acrylic Resin ◽

Denture Base ◽

Denture Stomatitis ◽

Significant Difference ◽

Almost All

Introduction: Heat-polymerized acrylic resin is used to manufacture almost all denture bases. One of the disadvantages of acrylic resin is its porosity and surface roughness; food scraps attach easily. If the dentures are not cleaned, they become a place for microbial species development and cause denture stomatitis, with Staphylococcus aureus and Candida albicans as the major etiologic agents. Adding nanoparticles of titanium dioxide to heat-polymerized acrylic resin may provide antimicrobial activity to the acrylic resin base. Objectives: This study aimed to determine whether adding TiO2 nanoparticles to heat-polymerized acrylic resin denture base materials affected the amounts of Staphylococcus aureus and Candida albicans. Methods: The samples in this study used heat-polymerized acrylic resin without the addition of TiO2 nanoparticles and with the addition of 2%, 3%, and 4% TiO2 nanoparticles on test bar sizes of 10 mm × 10 mm × 1 mm. There were 48 total test samples: 24 of Staphylococcus aureus and 24 of Candida albicans. The obtained data were analyzed by a one-way ANOVA test. Results: The results showed that adding TiO2 nanoparticles influenced the amount of Staphylococcus aureus (p<0.05) and Candida albicans (p<0.05). A least significant difference test showed differences of effect in the amounts of Staphylococcus aureus when TiO2 concentrations of 2%, 3%, and 4% were added to heat-polymerized acrylic resin denture base and there were differences in effect to amounts of Candida albicans when TiO2 concentrations of 2%, 3%, and 4% were added to heat-polymerized acrylic resin denture base. Conclusion: Adding TiO2 nanoparticles to heat-polymerized acrylic resin denture base material affects the amounts of Staphylococcus aureus and Candida albicans.

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