ANALYSIS OF MT2A AND MT3 GENE EXPRESSION IN RAT'S LIVER AND KIDNEY IN RESPONSE TO CADMIUM CHLORIDE POISONING

The aim of this study was to investigate the expression of metallothionein genes in the liver and kidneys of rats with acute cadmium poisoning.Simulation of poisoning with cadmium chloride was carried out on white outbred female rats, divided into 4 groups depending on the dose of the injected toxicant. RNA samples isolated from rat liver and kidneys were used as research materials.The multiplicity of expression of the MT3 gene in the kidneys increased at the lowest dose of CdCl2 , which was used in this experiment (0.029 mg / kg); with increasing dosage, the expression level decreased, but not lower than the control values. Analysis of the expression of the same gene in the liver showed a tendency towards a decrease in the content of transcripts with increasing dose. The frequency of expression of the MT2A gene at higher doses of CdCl2 increased both in the liver and in the kidneys.In the present work, statistically significant dose-dependent changes in the expression multiplicity of metallothionein genes were detected 24 hours after CdCl2 administration. The revealed differences in the level of transcriptional activity of metallothionein genes require further investigation, since there are probably differences in the level of gene expression at earlier and later periods of toxicant action.

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Regulation of rat Na+/Pi cotransporter-1 gene expression: the roles of glucose and insulin

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.1996.271.6.e1021 ◽

1996 ◽

Vol 271 (6) ◽

pp. E1021-E1028 ◽

Cited By ~ 7

Author(s):

H. Li ◽

P. Ren ◽

M. Onwochei ◽

R. J. Ruch ◽

Z. Xie

Keyword(s):

Gene Expression ◽

Glucose Metabolism ◽

Primary Culture ◽

Rat Liver ◽

Inorganic Phosphate ◽

Rat Hepatocytes ◽

Cdna Clones ◽

Homeostatic Regulation ◽

Liver And Kidney ◽

Streptozotocin Induced Diabetes

Cytosolic inorganic phosphate (P(i)) is important for glucose metabolism. It plays a role in homeostatic regulation of glucose by insulin and glucagon. Recently, we isolated two cDNA clones for rat Na+/P(i) cotransporter-1 (rNaPi-1) and demonstrated that they are expressed primarily in the rat liver and kidney. We now report that the expression of rNaPi-1 in these tissues is regulated by fasting and streptozotocin-induced diabetes. Using rat hepatocytes in primary culture, we also demonstrate that glucose and insulin upregulate rNaPi-1 expression, whereas glucagon and elevated intracellular adenosine 3',5'-cyclic monophosphate levels downregulate its expression. Because 2-deoxyglucose exhibits no effect on rNaPi-1 gene expression, we suggest that some metabolite accumulated during glucose metabolism may be responsible for the effects of glucose and insulin on rNaPi-1 gene expression. Our data also reveal that other known Na+/P(i) cotransporter genes, NaPi-2 and Ram-1 (a receptor for amphotropic murine retrovirus), are not regulated by insulin and glucose. It is therefore proposed that various subtypes of Na+/P(i) cotransporters are differentially regulated and that each subtype may be involved in a specific cellular function, rNaPi-1 may be responsible for Pi uptake by liver and kidney for glucose metabolism, whereas NaPi-2 may play a key role in P(i) reabsorption in the kidney.

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Regulation of heme oxygenase gene expression by cobalt in rat liver and kidney

European Journal of Biochemistry ◽

10.1111/j.1432-1033.1990.tb19263.x ◽

1990 ◽

Vol 192 (3) ◽

pp. 577-582 ◽

Cited By ~ 45

Author(s):

Jane H.-C. LIN ◽

Patricio VILLALON ◽

Pavel MARTASEK ◽

Nader G. ABRAHAM

Keyword(s):

Gene Expression ◽

Heme Oxygenase ◽

Rat Liver ◽

Liver And Kidney

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The Effectiveness of Dietary Byproduct Antioxidants on Induced CYP Genes Expression and Histological Alteration in Piglets Liver and Kidney Fed with Aflatoxin B1 and Ochratoxin A

Toxins ◽

10.3390/toxins13020148 ◽

2021 ◽

Vol 13 (2) ◽

pp. 148

Author(s):

Roua Gabriela Popescu ◽

Cristina Bulgaru ◽

Arabela Untea ◽

Mihaela Vlassa ◽

Miuta Filip ◽

...

Keyword(s):

Gene Expression ◽

Ochratoxin A ◽

Aflatoxin B1 ◽

Basal Diet ◽

Sea Buckthorn ◽

Expression Level ◽

Control Group ◽

Tissue Samples ◽

Perform Gene Expression ◽

Liver And Kidney

The purpose of this study was to investigate the potential of a byproduct mixture derived from grapeseed and sea buckthorn oil industry to mitigate the harmful damage produced by ochratoxin A and aflatoxin B1 at hepatic and renal level in piglets after weaning. Forty cross-bred TOPIGS-40 hybrid piglets after weaning were assigned to three experimental groups (E1, E2, E3) and one control group (C), and fed with experimental diets for 30 days. The basal diet was served as a control and contained normal compound feed for starter piglets without mycotoxins. The experimental groups were fed as follows: E1—basal diet plus a mixture (1:1) of two byproducts (grapeseed and sea buckthorn meal); E2—the basal diet experimentally contaminated with mycotoxins (479 ppb OTA and 62ppb AFB1); and E3—basal diet containing 5% of the mixture (1:1) of grapeseed and sea buckthorn meal and contaminated with the mix of OTA and AFB1. After 4 weeks, the animals were slaughtered, and tissue samples were taken from liver and kidney in order to perform gene expression and histological analysis. The gene expression analysis showed that when weaned piglets were fed with contaminated diet, the expression of most analyzed genes was downregulated. Among the CYP450 family, CYP1A2 was the gene with the highest downregulation. According to these results, in liver, we found that mycotoxins induced histomorphological alterations in liver and kidney and had an effect on the expression level of CYP1A2, CYP2A19, CYP2E1, and CYP3A29, but we did not detect important changes in the expression level of CY4A24, MRP2 and GSTA1 genes.

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Analysis of the Mouse CSF-1 Gene Promoter in a Transgenic Mouse Model1

Journal of Histochemistry & Cytochemistry ◽

10.1177/002215540305100709 ◽

2003 ◽

Vol 51 (7) ◽

pp. 941-949 ◽

Cited By ~ 6

Author(s):

Sherry L. Abboud ◽

Maria Bunegin ◽

Nandini Ghosh-Choudhury ◽

Kathleen Woodruff

Keyword(s):

Gene Expression ◽

Transgenic Mice ◽

Transgene Expression ◽

Transcriptional Activity ◽

Molecular Mechanisms ◽

Tissue Expression ◽

Cellular Level ◽

Liver And Kidney

CSF-1 stimulates monocyte and osteoclast populations. However, the molecular mechanisms involved in regulating CSF-1 gene expression are unclear. To identify regulatory regions that control normal CSF-1 gene expression, a −774/+183-bp fragment of the murine CSF-1 promoter was analyzed in vitro and in vivo. Transcriptional activity was high in cultured osteoblasts that express CSF-1 mRNA compared to ARH-77 B cells that lack CSF-1 gene expression. Transient transfection of osteoblasts with promoter deletion constructs showed that the −774-bp fragment conferred the highest transcriptional activity and contained activator and repressor sequences. To assess the ability of the CSF-1 promoter to confer normal tissue expression of CSF-1, transgenic mice containing the −774/+183-bp region driving the E. coli β-galactosidase (lacZ) reporter gene were generated. β-Gal analysis of whole tissue extracts showed transgene expression in all tissues tested except liver and kidney. At the cellular level, the pattern of β-gal expression in the spleen, thymus, bone, lung, and testes of adult transgenic mice mimicked normal endogenous CSF-1 mRNA expression in non-transgenic littermates detected by in situ hybridization. This region also directed appropriate transgene expression to sites in other tissues known to synthesize CSF-1, with the exception of the liver and kidney. These findings indicate that the −774-bp fragment contains cis-acting elements sufficient to direct CSF-1 gene expression in many tissues. CSF-1 promoter/lacZ mice may be useful for studying the transcriptional mechanisms involved in regulating CSF-1 gene expression in tissues throughout development.

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Differential regulation of prolactin receptor mRNA expression in rat liver and kidney by testosterone and oestradiol

Journal of Endocrinology ◽

10.1677/joe.0.1430383 ◽

1994 ◽

Vol 143 (2) ◽

pp. 383-392 ◽

Cited By ~ 9

Author(s):

K Sakaguchi ◽

T Ohkubo ◽

T Sugiyama ◽

M Tanaka ◽

H Ushiro ◽

...

Keyword(s):

Mrna Expression ◽

Rat Liver ◽

Short Form ◽

Differential Regulation ◽

Female Rats ◽

Male Rats ◽

Mrna Levels ◽

Female Rat ◽

Long Form ◽

Liver And Kidney

Abstract Prolactin (PRL) exerts a wide variety of physiological effects on mammalian tissues through its receptor (PRL-R) on the target cells. PRL-R in rat tissue consists of two isoforms, the long and the short form, and the regulatory mechanisms of their mRNA expression in tissues are complex and diverse. The present study reports the differential regulation of PRL-R mRNA expression in rat liver and kidney by testosterone and oestradiol. Using Northern blot analysis, short form PRL-R mRNA was clearly detected in female rat liver and male rat kidney, and long form PRL-R mRNA was faintly observed only in female rat liver. However, the reverse transcription-polymerase chain reaction method enabled efficient analysis of mRNA levels in short and long forms of PRL-R in the liver and kidney of both male and female rats. The mRNA levels for the long and short forms of PRL-R were depressed in the liver of male rats but not in that from female rats during sexual maturation. Castration of male rats resulted in the induction of the mRNAs for these two forms of PRL-R in the liver. Testosterone, but not oestradiol, completely blocked the induction by castration of liver PRL-R gene expression. In kidney, in contrast, mRNA levels for both forms of PRL-R were depressed in female rats but not in male rats after sexual maturation. Administration of oestradiol, but not of testosterone, caused marked repression of short form PRL-R mRNA, particularly in the kidney of male rats. The levels of long form PRL-R mRNA in the kidney was less affected by the administration of oestradiol. These results have suggested that the expression of PRL-R mRNAs in rat liver and kidney is differentially regulated by testosterone and oestrogen. Journal of Endocrinology (1994) 143, 383–392

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The chemical form of cadmium in microsomal and mitochondrial fractions from rat liver and kidney after long term administration of cadmium chloride

Toxicology Letters ◽

10.1016/0378-4274(81)90052-7 ◽

1981 ◽

Vol 7 (4-5) ◽

pp. 297-304 ◽

Cited By ~ 9

Author(s):

Hidemi Nakazawa ◽

Yasuo Masuzawa ◽

Keizo Waku

Keyword(s):

Rat Liver ◽

Cadmium Chloride ◽

Chemical Form ◽

Mitochondrial Fractions ◽

Term Administration ◽

Liver And Kidney

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THE EFFECT OF PARATHION ON RAT LIVER AND KIDNEY CARBOXYLESTERASES

Canadian Journal of Biochemistry ◽

10.1139/o65-179 ◽

1965 ◽

Vol 43 (10) ◽

pp. 1625-1632 ◽

Cited By ~ 4

Author(s):

Sheila I. Read ◽

W. P. McKinley

Keyword(s):

Vitamin A ◽

Rat Liver ◽

Young Female ◽

Female Rats ◽

Male Rats ◽

Vitamin A Supplementation ◽

Different Ages ◽

Liver And Kidney

Rats of different ages were fed a diet containing 10 p.p.m. parathion with or without vitamin A supplementation for various periods of time, and the effects on liver and kidney carboxylesterases were measured.Marked inhibition of carboxylesterases was observed shortly after the parathion diet was introduced. The degree of inhibition was not altered appreciably by continued feeding of the diet containing parathion. Young female rats showed some recovery of liver carboxylesterases on continued feeding of the parathion diet. After removal of vitamin A from the diet, the levels of liver carboxylesterases of male rats fed parathion increased appreciably.

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