Identifying cryptic species in pollinating-fig wasps by PCR-RFLP on mtDNACOIgene

SummaryResistance to activated protein C is the most common hereditary cause for thrombosis and significantly linked to factor V Leiden. In this study, primers were designed to identify the factor V mutation by allele-specific PCR amplification. 126 patients with thromboembolic events were analysed using this technique, PCR-RFLP and direct sequencing. The concordance between these techniques was 100%. In 27 patients a heterozygous factor VGln506 mutation was detected, whereas one patient with recurrent thromboembolism was homozygous for the point mutation. Due to its time- and cost-saving features allele-specific amplification should be considered for screening of factor VGln506.

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PCR-RFLP Detection od PAI-2 Gene Variants: Prevelence in Ethnic Groups and Disease Relationship in patients Undergoing corony Angiography

Thrombosis and Haemostasis ◽

10.1055/s-0038-1656084 ◽

1997 ◽

Vol 77 (05) ◽

pp. 0955-0958 ◽

Cited By ~ 8

Author(s):

Carole A Foy ◽

Peter J Grant

Keyword(s):

Gene Variants ◽

Genotype Distribution ◽

Pima Indians ◽

Significant Difference ◽

Pcr Rflp ◽

History Of ◽

Caucasian Patients ◽

Clinical Conditions ◽

Rflp Assay ◽

Coronary Atheroma

SummaryPAI-2 is a fibrinolytic inhibitor produced predominantly by monocytes. Most PAI-2 is intracellular making study in clinical conditions difficult. Abnormalities in production may be associated with inflammation and fibrinolysis at sites of tissue damage such as the atherosclerotic plaque.PAI-2 gene variants have been described: variant A consists of Asn120, Asn404 and Ser413 and variant B consists of Asp120, Lys404 and Cys413. We designed a PCR-RFLP assay using primers spanning the region containing Asn/Lys404 and Ser/Cys413. Variant B contains an Mwol restriction site. We analysed 302 Pima Indians and 286 healthy Caucasian volunteers. To investigate relationships between genotype and vascular disease we analysed 333 Caucasian patients undergoing coronary angiography.Gene variant B was more common in the Pimas than in Caucasians (p <0.0001). There was no significant difference in genotype distribution between the volunteers and patients. In the patients there was no association between genotype and either a history of MI or extent of coronary atheroma.

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Differentiation Of Clarias Batrachus, C. Gariepinus And Heteropneustes Fossilis By Pcr-Sequencing Of Mitochondrial 16s Rrna Gene

Journal of the Asiatic Society of Bangladesh Science ◽

10.3329/jasbs.v41i1.46190 ◽

2015 ◽

Vol 41 (1) ◽

pp. 51-58

Author(s):

Mohammad Shamimul Alam ◽

Hawa Jahan ◽

Rowshan Ara Begum ◽

Reza M Shahjahan

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Fish Larva ◽

16S Rrna Genes ◽

Rrna Genes ◽

Rrna Gene ◽

Valuable Insight ◽

Multiple Sequence ◽

Pcr Rflp ◽

Alternative Means

Heteropneustesfossilis, Clariasbatrachus and C. gariepinus are three major catfishes ofecological and economic importance. Identification of these fish species becomes aproblem when the usual external morphological features of the fish are lost or removed,such as in canned fish. Also, newly hatched fish larva is often difficult to identify. PCRsequencingprovides accurate alternative means of identification of individuals at specieslevel. So, 16S rRNA genes of three locally collected catfishes were sequenced after PCRamplification and compared with the same gene sequences available from othergeographical regions. Multiple sequence alignment of the 16S rRNA gene fragments ofthe catfish species has revealed polymorphic sites which can be used to differentiate thesethree species from one another and will provide valuable insight in choosing appropriaterestriction enzymes for PCR-RFLP based identification in future. Asiat. Soc. Bangladesh, Sci. 41(1): 51-58, June 2015

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Genetic Variation in Yellowfin Tuna Thunnus Albacares (Bonnaterre, 1788) Along Indian Coast Using Pcr-Rflp Analysis of Mitochondrial Dna D-Loop Region

International Journal of Scientific Research ◽

10.15373/22778179/jan2014/8 ◽

2012 ◽

Vol 3 (1) ◽

pp. 25-30

Author(s):

Swaraj Priyaranjan Kunal ◽

◽

Girish Kumar Girish Kumar ◽

Maria Rosalia Menezes

Keyword(s):

Mitochondrial Dna ◽

Genetic Variation ◽

Rflp Analysis ◽

Yellowfin Tuna ◽

Thunnus Albacares ◽

Indian Coast ◽

Loop Region ◽

Pcr Rflp ◽

D Loop

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Identification of sibling species in the genus Eucyclops from water-bodies of Ukraine and Russia using crossbreeding studies

Ecology and Noospherology ◽

10.15421/031406 ◽

2014 ◽

Vol 25 (1-2) ◽

pp. 61-68 ◽

Cited By ~ 1

Author(s):

V. I. Monchenko ◽

L. P. Gaponova ◽

V. R. Alekseev

Keyword(s):

Cryptic Species ◽

River Basin ◽

Black Sea ◽

Species Complex ◽

River Basins ◽

Water Bodies ◽

Similar Species ◽

Basin Water ◽

Different Populations ◽

Baltic Sea Basin

Crossbreeding experiments were used to estimate cryptic species in water bodies of Ukraine and Russia because the most useful criterion in species independence is reproductive isolation. The problem of cryptic species in the genus Eucyclops was examined using interpopulation crosses of populations collected from Baltic Sea basin (pond of Strelka river basin) and Black Sea basin (water-reservoires of Dnieper, Dniester and Danube rivers basins). The results of reciprocal crosses in Eucyclops serrulatus-group are shown that E. serrulatus from different populations but from water bodies belonging to the same river basin crossed each others successfully. The interpopulation crosses of E. serrulatus populations collected from different river basins (Dnipro, Danube and Dniester river basins) were sterile. In this group of experiments we assigned evidence of sterility to four categories: 1) incomplete copulation or absence of copulation; 2) nonviable eggs; 3) absence of egg membranes or egg sacs 4) empty egg membranes. These crossbreeding studies suggest the presence of cryptic species in the E. serrulatus inhabiting ecologically different populations in many parts of its range. The same crossbreeding experiments were carries out between Eucyclops serrulatus and morphological similar species – Eucyclops macruroides from Baltic and Black Sea basins. The reciprocal crossings between these two species were sterile. Thus taxonomic heterogeneity among species of genus Eucyclops lower in E. macruroides than in E. serrulatus. The interpopulation crosses of E. macruroides populations collected from distant part of range were fertile. These crossbreeding studies suggest that E. macruroides species complex was evaluated as more stable than E. serrulatus species complex.

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