Application of three automatic blood group testing instruments in cross matching test

2013 ◽  
Vol 33 (1) ◽  
pp. 92-94
Author(s):  
Bo CHEN ◽  
Chun-hong GE ◽  
Yan LIN ◽  
Yin LIU ◽  
Ye ZHOU ◽  
...  
Keyword(s):  
Blood Group ◽  
Group Testing ◽  
Matching Test ◽  
Testing Instruments ◽  
Cross Matching

Selection of Blood (Packed RBCs) for Transfusion in Newborn Baby up to the Age of 4 Months

2012 ◽  
Vol 1 (1) ◽  
pp. 36-40
Author(s):  
Ghulam Mostafa Khan
Keyword(s):  
Blood Group ◽  
Whole Blood ◽  
Placental Transfer ◽  
Newborn Baby ◽  
Older Children ◽  
Proper Selection ◽  
Group A ◽  
Cross Matching ◽  
Packed Rbcs ◽  
Selection Of

Proper selection of donor’s blood group is essential to prevent transfusion hazards. It is known that ABO antigen is fully developed at birth but the newborn baby does not produce ABO antibodies until 3 to 6 months of age. The ABO antibodies present in the serum of newborn babies are derived from mother’s blood due to placental transfer. So the blood group of the newborn baby is done by ABO antigen grouping (forward grouping) only, antibody grouping (reverse grouping) is not required. In case of transfusion of blood in newborn under 4 months of age, cross-matching of donor’s blood is done with the mother’s blood if it is available. We know, recipient’s same group of blood is always preferable in case of transfusion in adults or older children. But selection of blood for transfusion in the infants under 4 months of age depends on the mother’s blood group as well. If the mother’s blood group differs from the infant’s blood group, the infant’s same group of blood may not be selected for transfusion. For example, if the mother’s blood group is “O” and the newborn blood group is “A” or “B”, infant’s same group “A” or “B” group blood could not be transfused, because the anti-A & anti-B antibodies can be derived in the infant’s serum from mother’s blood which may react with the “A” or “B” antigen of the donor’s blood. In this case “O” group packed RBCs should be selected for transfusion. “O” group whole blood may contain IgG anti-A and anti-B antibodies in the plasma which can react with the “A” or “B” antigen of the infant’s blood. So to avoid anti-A & anti-B antibodies in “O” group, plasma should be discarded and the packed RBCs should be transfused. In case of Rh-negative mother with Rh positive baby, Rh antibody may develop in mother’s blood and Rh antibody may enter into baby’s circulation, in this case the infant should be transfused with Rh-negative blood to avoid Rh antigen & antibody reaction. So for the selection of blood for transfusion in newborn baby up to the age of 4 months mother’s blood group is important to select the appropriate blood. DOI: http://dx.doi.org/10.3329/jemc.v1i1.11138J Enam Med Col 2011; 1(1): 36-40

scholarly journals 2-mercaptoethanol (2-ME)-based IATs or polybrene method mitigates the interference of anti-CD38 immunotherapy on blood compatibility tests

2020 ◽  
Author(s):  
Ye Zhou ◽  
Leiyu Chen ◽  
Tianshu Jiang ◽  
Liangfeng Fan ◽  
Hang Lei ◽  
...  
Keyword(s):  
Clinical Laboratory ◽  
Blood Compatibility ◽  
Positive Control ◽  
Routine Practice ◽  
Blood Group Antigens ◽  
Time Saving ◽  
Antibody Screening ◽  
Matching Test ◽  
Working Solution ◽  
Cross Matching

Abstract Background Treating red blood cells (RBCs) with dithiothreitol (DTT) is a wildly-recommended to overcome the interference of anti-CD38 immunotherapy with blood compatibility testing. Nevertheless, DTT can be hard to obtain in clinical laboratory, while its use in routine practice may be time-consuming. In the following study, we explored the feasibility of using a commercial 2-mercaptoethanol (2-ME) working solution or the time-saving polybrene method to mitigate the daratumumab (DARA) interference. Materials and Methods Antibody screening and cross-matching were performed using 2-ME or DTT-based indirect antiglobulin tests (IATs) and polybrene method (human IgG anti-E same IATs titer as DARA as positive control) on 37 samples, and these samples were from patients enrolled in the “Several methods resolve the interference of anti-CD38 monoclonal antibody on blood compatibility tests” clinical trial (www.chictr.org.cn identifier: ChiCTR2000040761). Most clinically important blood group antigens on RBCs were detected after treatment with 2-ME or DTT. Hemoglobin values were compared after 69 units RBCs were transfused with compatible cross-matching results by a 2-ME-based method or polybrene test. Results Treating RBCs with 2-ME eliminates the DARA interference with the antibody screening or cross-matching; yet, K antigen is denatured during treatment. DARA with 2+ agglutinations of anti-E control does not interfere with antibody screening and cross-matching via polybrene method. After RBCs transfusion with a negative cross-matching test by 2-ME-based IATs or polybrene method, hemoglobin significantly increased without adverse transfusion reactions. Conclusion 2-ME-based IATs or polybrene method could be used to mitigate DARA interference as DTT.

scholarly journals Blood Group Detection using Matlab

2021 ◽  
Vol 9 (VI) ◽  
pp. 3089-3093
Author(s):  
Sujatha C. N
Keyword(s):  
Image Processing ◽  
Blood Group ◽  
Human Error ◽  
Group Identification ◽  
Group Testing ◽  
Emergency Situation ◽  
Blood Type ◽  
Morphological Operations ◽  
Laboratory Staff ◽  
Emergency Situations

Blood group testing is one of the vital tasks in the area of medicine, in which it is very important during emergency situation before victim requires blood transfusion. Presently, the blood tests are conducted manually by laboratory staff members, which is time consuming process in the emergency situations. Blood group identification within shortest possible time without any human error is an important factor and very much essential. Image processing paves a way in determining blood type without human intervention. Images which are captured using high resolution microscopic camera during the blood slide test in the laboratory which are used for blood type evaluation. The image processing techniques which include thresholding and morphological operations are used. The blood image is separated into sample wise and blood type is decided based on the agglutination effects in those sample images. This project facilitates the identification of blood group even by common people who are unaware of the blood typing procedure.

scholarly journals Blood group testing in developing countries: an ignored concern

2010 ◽  
Vol 4 (12) ◽  
pp. 852-853
Author(s):  
Divya Talwar ◽  
Amit Arora
Keyword(s):  
Developing Countries ◽  
Blood Group ◽  
Full Text ◽  
Group Testing

This item has no abstract: use the links below to access the full text.

Another Pitfall in Blood Group Testing for Nonpaternity

Transfusion ◽  
1973 ◽  
Vol 13 (4) ◽  
pp. 231-232
Author(s):  
L. N. Sussman ◽  
R. Solomon
Keyword(s):  
Blood Group ◽  
Group Testing

Method for ABO Blood Group Testing Using a General-Purpose Automated Biochemical Analyzer

2019 ◽  
Vol 65 (06/2019) ◽  
Author(s):  
Satoshi Tada ◽  
Shoichi Kanayama ◽  
Akemi Miyagawa ◽  
Koji Murao
Keyword(s):  
Blood Group ◽  
Group Testing ◽  
General Purpose ◽  
Abo Blood Group ◽  
Biochemical Analyzer

Aroma Impact Compounds in Three Citrus Oils: Cross-matching Test and Correspondence Analysis Approach

2006 ◽  
Vol 71 (1) ◽  
pp. S54-S58 ◽  
Author(s):  
Masahiro Chida ◽  
Keiko Yamashita ◽  
Yuri Izumiya ◽  
Kokichi Watanabe ◽  
Hirotoshi Tamura
Keyword(s):  
Correspondence Analysis ◽  
Analysis Approach ◽  
Matching Test ◽  
Cross Matching

ANTI-GLOBULIN CROSS-MATCHING TEST

The Lancet ◽  
1958 ◽  
Vol 271 (7022) ◽  
pp. 664-667 ◽  
Author(s):  
E.E. French
Keyword(s):  
Matching Test ◽  
Cross Matching

scholarly journals Phenotypic and Genetic Characterization for Incompatible Cross-Match Cases in the Feline AB Blood Group System

2021 ◽  
Vol 8 ◽  
Author(s):  
Yumiko Uno ◽  
Masami Yaguchi ◽  
Tasuku Kobayashi ◽  
Eri Onozawa ◽  
Kazuhiko Ochiai ◽  
...  
Keyword(s):  
Blood Group ◽  
Blood Type ◽  
Blood Group System ◽  
Transfusion Medicine ◽  
Type B ◽  
Blood Typing ◽  
Group System ◽  
Blood Types ◽  
Incompatible Cross ◽  
Cross Matching

The feline AB blood group system (blood types A, B, and AB) encoding the cytidine monophosphate-N-acetylneuraminic acid hydroxylase (CMAH) gene is the most significant in transfusion medicine and hemolysis of the newborn for cats. Blood typing and cross-matching in pre-transfusion testing are crucial to determining blood compatibility and thus prevent hemolytic transfusion reactions. We here performed serological and genetic investigations to characterize blood samples from cats with discordant results for card agglutination (CARD) and the alloantibody agglutination test for blood typing in two cats (subjects K and R). Subject K showed incompatible cross-matching in pre-transfusion testing. Red blood cells from subjects K and R determined blood type B from the CARD method showed blood type AB by alloanti-A and alloanti-B antibodies in agglutination testing. Genomic DNA sequencing of the coding region (exons 1a to 14) for the cat CMAH gene showed that subject K had four mutations with heterozygosity at c.139C>T, c.179G>T, c.327A>C, and c.364C>T. Similarly, the CMAH gene of subject R carried six mutations with heterozygosity at c.142G>A, c.187A>G, c.268T>A, c.327A>C, c.773G>A and c.1603G>A, representing a new diplotype including a novel synonymous single nucleotide polymorphism (SNP) in exon 7 (c.773 G>A: Arg258Gln). The CMAH diplotype in subjects K and R was different from major diplotype in blood type B cats. This study is the first to report CMAH variants in cats with discordant blood types between CARD and TUBE methods. These results could assist in the classification of feline AB blood types for transfusion medicine to avoid blood incompatibilities.

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