Formation of Antibodies for Blood-Group Testing by Immunization of Healthy Donors

Author(s):  
Gertraud Pilgrim
Author(s):  
Sujatha C. N

Blood group testing is one of the vital tasks in the area of medicine, in which it is very important during emergency situation before victim requires blood transfusion. Presently, the blood tests are conducted manually by laboratory staff members, which is time consuming process in the emergency situations. Blood group identification within shortest possible time without any human error is an important factor and very much essential. Image processing paves a way in determining blood type without human intervention. Images which are captured using high resolution microscopic camera during the blood slide test in the laboratory which are used for blood type evaluation. The image processing techniques which include thresholding and morphological operations are used. The blood image is separated into sample wise and blood type is decided based on the agglutination effects in those sample images. This project facilitates the identification of blood group even by common people who are unaware of the blood typing procedure.


2010 ◽  
Vol 4 (12) ◽  
pp. 852-853
Author(s):  
Divya Talwar ◽  
Amit Arora

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Transfusion ◽  
1973 ◽  
Vol 13 (4) ◽  
pp. 231-232
Author(s):  
L. N. Sussman ◽  
R. Solomon
Keyword(s):  

2019 ◽  
Vol 65 (06/2019) ◽  
Author(s):  
Satoshi Tada ◽  
Shoichi Kanayama ◽  
Akemi Miyagawa ◽  
Koji Murao

2020 ◽  
Vol 7 (6) ◽  
pp. 505-508
Author(s):  
Tuğçe Nur Yigenoğlu ◽  
Mehmet Bakırtaş ◽  
Semih Başcı ◽  
Bahar Uncu Ulu ◽  
Derya Şahin ◽  
...  

Objective: Many factors, including advanced age and female gender, have been identified as negative factors for peripheric blood hematopoietic stem cell (HSC) mobilization. Similarly, blood group antigens may have an effect on the release of HSCs from the bone marrow niche into the periphery. We aimed to study the effect of ABO and Rh blood groups on peripheral blood HSC mobilization in healthy donors. Material and Method: The data of 314 healthy donors who underwent peripheric blood HSC mobilization in our center were analyzed retrospectively. Results: The number of CD34+ cells collected on the first day and in total was the least in donors with blood group A. A statistically significant relation was found between ABO blood groups and the number of CD34+ cells collected on the first day and in total. No relation was found between Rh positivity and the number of CD34+ cells collected. Conclusion: According to our research in the literature, this is the first study that investigates whether blood groups have an effect on the release of HSCs from the bone marrow niche into the periphery and we observed that blood group A is a negative risk factor for peripheric blood HSC mobilization.


2013 ◽  
Vol 33 (1) ◽  
pp. 92-94
Author(s):  
Bo CHEN ◽  
Chun-hong GE ◽  
Yan LIN ◽  
Yin LIU ◽  
Ye ZHOU ◽  
...  

2021 ◽  
Vol 66 (11) ◽  
pp. 661-665
Author(s):  
Sergey Ivanovich Donskov ◽  
A. Yu. Bulanov ◽  
I. B. Simarova ◽  
V. V. Belyakova ◽  
O. A. Mayorova ◽  
...  

The frequency of AB0 and Rhesus blood groups was studied in 12120 patients with COVID-19, 5180 convalescent plasma donors and 118801 healthy donors from Moscow, Smolensk, Yakutsk, Minsk and Gomel. In infected individuals, the frequency of blood group A was significantly higher than in uninfected individuals (41,54 % and 34,39 % respectively, p<0,05), and the frequency of blood group 0, on the contrary, was significantly lower (27,69 % and 36,71 %, p<0,05). The frequency of blood group A was particularly high among patients who died from ARVI COVID-19 - 45,51 % vs. 34,39 %, p=0,008. In some groups of patients, there was a decrease in the frequency of Rh-negative individuals (2,23 % vs. 8,30 %, p<0,001).


Author(s):  
Debasish Mishra ◽  
Pankaj Parida ◽  
Smita Mahapatra ◽  
Binay Bhusan Sahoo

Background: Blood grouping consists of both forward grouping; reverse grouping and both procedures should agree with each other.A blood group discrepancy exists when results of red cell testing do not agree with serum testing, usually due to unexpected negative or positive results in either forward or reverse typing. ABO and Rh blood group discrepancy is associated with incompatible transfusion reaction.Blood group discrepancy should be resolved before transfusion and blood group to be properly labeled to prevent transfusion reaction.Methods: A prospective study was carried in SCB blood bank which is under the Department of Transfusion Medicine, SCB Medical College and Hospital, Cuttack, Odisha from January 2015 to October-2016. Total 25,559 blood samples of patients were included in the study and hemolysed samples excluded. The ABO and Rh D typing was done by tube technique using monoclonal IgM (Tulip Diagnostic P Ltd.) Anti-A, Anti-B, Anti-D and pooled A, B and O cell.Results: A total of 25,559 blood group testing were done where we found 57 blood group discrepancies with overall frequency was 0.22%. Out of 57 discrepancies we were found 20 (35.09%) cases of technical error and 37 (64.91%) cases of sample related error. Among these sample related problems, we found weak/missing antibody, weak antigen expression, rouleaux, cold autoantibodies, cold alloantibodies, Bombay phenotype with the frequency of 13.51%, 2.70%, 2.70%, 54.06%, 8.11%, 18.92% respectively.Conclusions: Mistyping either a donor or a recipient can lead to transfusion with ABO-incompatible blood, which can result in severe hemolysis and may even result in the death of the recipient. Any discrepancy between forward and reverse blood grouping methods should be resolved before transfusion of blood components.


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