Expression profiles of gonadotropin receptors during ovary development in the orange-spotted grouper (Epinephelus coioides)

2013 ◽  
Vol 19 (6) ◽  
pp. 915-922 ◽  
Author(s):  
Xuesong HU ◽  
Xiaochun LIU ◽  
Yong ZHANG ◽  
Shuisheng LI ◽  
Huapu CHEN ◽  
...  
Keyword(s):  
Expression Profiles ◽  
Ovary Development ◽  
Epinephelus Coioides ◽  
Gonadotropin Receptors

scholarly journals Genome-wide transcriptome profiling uncovers differential miRNAs and lncRNAs in ovaries of Hu sheep at different developmental stages

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Samina Shabbir ◽  
Prerona Boruah ◽  
Lingli Xie ◽  
Muhammad Fakhar-e-Alam Kulyar ◽  
Mohsin Nawaz ◽  
...  
Keyword(s):  
Target Genes ◽  
Expression Profiles ◽  
Ovarian Follicle ◽  
Follicular Development ◽  
Differentially Expressed ◽  
Ovary Development ◽  
Estrogen Metabolism ◽  
Genome Wide ◽  
Micro Rnas ◽  
Hu Sheep

AbstractOvary development is an important determinant of the procreative capacity of female animals. Here, we performed genome-wide sequencing of long non-coding RNAs (lncRNAs) and mRNAs on ovaries of 1, 3 and 8 months old Hu sheep to assess their expression profiles and roles in ovarian development. We identified 37,309 lncRNAs, 45,404 messenger RNAs (mRNAs) and 330 novel micro RNAs (miRNAs) from the transcriptomic analysis. Six thousand, seven hundred and sixteen (6716) mRNAs and 1972 lncRNAs were significantly and differentially expressed in ovaries of 1 month and 3 months old Hu sheep (H1 vs H3). These mRNAs and target genes of lncRNAs were primarily enriched in the TGF-β and PI3K-Akt signalling pathways which are closely associated with ovarian follicular development and steroid hormone biosynthesis regulation. We identified MSTRG.162061.1, MSTRG.222844.7, MSTRG.335777.1, MSTRG.334059.16, MSTRG.188947.6 and MSTRG.24344.3 as vital genes in ovary development by regulating CTNNB1, CCNA2, CDK2, CDC20, CDK1 and EGFR expressions. A total of 2903 mRNAs and 636 lncRNAs were differentially expressed in 3 and 8 months old ovaries of Hu sheep (H3 vs H8); and were predominantly enriched in PI3K-Akt, progesterone-mediated oocyte maturation, estrogen metabolism, ovulation from the ovarian follicle and oogenesis pathways. These lncRNAs were also found to regulate FGF7, PRLR, PTK2, AMH and INHBA expressions during follicular development. Our result indicates the identified genes participate in the development of the final stages of follicles and ovary development in Hu sheep.

Molecular cloning of two gonadotropin receptors and their distinct mRNA expression profiles in daily oogenesis of the wrasse Pseudolabrus sieboldi

2011 ◽  
Vol 172 (2) ◽  
pp. 268-276 ◽  
Author(s):  
Hajime Kitano ◽  
Susumu Irie ◽  
Kohei Ohta ◽  
Toshiaki Hirai ◽  
Akihiko Yamaguchi ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Molecular Cloning ◽  
Expression Profiles ◽  
Gonadotropin Receptors

Differentially Expressed lncRNAs After the Activation of Primordial Follicles in Mouse

2018 ◽  
Vol 26 (8) ◽  
pp. 1094-1104
Author(s):  
Liping Zheng ◽  
Ruichen Luo ◽  
Tie Su ◽  
Liaoliao Hu ◽  
Fengxin Gao ◽  
...  
Keyword(s):  
Messenger Rna ◽  
Expression Profiles ◽  
Ovarian Follicle ◽  
Primordial Follicle ◽  
Differentially Expressed ◽  
Ovary Development ◽  
Primordial Follicles ◽  
Primordial Follicle Activation ◽  
Follicle Activation

The activation of primordial follicles is critical to ovarian follicle development, which directly influences female fertility and reproductive life span. Several studies have suggested a role for long noncoding RNAs (lncRNAs) in ovarian function. However, the precise involvement of lncRNAs in the initiation of primordial follicles is still unknown. Here, an in vitro culture model was used to investigate the roles of lncRNAs in primordial follicle activation. We found that primordial follicles in day 3 mouse ovaries were activated after culturing for 8 days in vitro, as indicated by ovarian morphology changes, increases in primary follicle number, and downregulation of mammalian Sterile 20-like kinase messenger RNA (mRNA) and upregulation of growth differentiation factor 9 mRNA. We next examined lncRNA expression profiles by RNA sequencing at the transcriptome level and found that among 60 078 lncRNAs, 6541 lncRNA were upregulated and 2135 lncRNA were downregulated in 3-day ovaries cultured for 8 days in vitro compared with ovaries from day 3 mice. We also found that 4171 mRNAs were upregulated and 1795 were downregulated in the cultured ovaries. Gene ontology and pathway analyses showed that the functions of differentially expressed lncRNA targets and mRNAs were closely linked with many processes and pathways related to ovary development, including cell proliferation and differentiation, developmental processes, and other signaling transduction pathways. Additionally, many novel identified lncRNAs showed inducible expression, suggesting that these lncRNAs may be good candidates for investigating mouse primordial follicle activation. This study provides a foundation for further exploring lncRNA-related mechanisms in the initiation of mouse primordial follicles.

Effect of bisphenol A exposure on the expressions of five ovary development related genes in the oriental river prawn, Macrobrachium nipponense (Decapoda, Palaemonidae)

Crustaceana ◽  
2018 ◽  
Vol 91 (3) ◽  
pp. 321-334
Author(s):  
Xiu Li ◽  
Fengying Zhang ◽  
Keji Jiang ◽  
Weihong Zhao ◽  
Ming Zhao ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Fresh Water ◽  
Manufacturing Industry ◽  
Expression Profiles ◽  
Ovary Development ◽  
Control Group ◽  
Production Volume ◽  
Macrobrachium Nipponense ◽  
Severe Problem ◽  
Oriental River Prawn

The oriental river prawn,Macrobrachium nipponense, is one of the important commercial shrimp species. Recently a severe problem of precocious maturation has greatly hampered this shrimp aquaculture industry. Bisphenol A (BPA), a high-production-volume chemical substance used in the plastic manufacturing industry, has been thought of as an endocrine disruptor on the developmental processes of animals. In this study, we investigated the effect of BPA exposure on the expressions of five ovary development related genes such as Mago nashi, Tsunagi, Gustavus, Ubc9, and Von Willebrand factor D-Kazal inM. nipponense. Five concentration gradients (5.01, 7.76, 12.06, 18.62 and 28.84 mg/l) of BPA were set and the ovaries of exposed prawns were collected at different time points for expression analysis. Compared with the control group at day 19, BPA had a two-phase effect: a stimulating effect under low concentrations from 5.01 to 12.06 mg/l, whereas a negative effect was noted at high concentrations from 12.06 to 28.84 mg/l. The expression profiles under different BPA concentrations significantly changed along with the extension of exposure time. The medium concentration of BPA (12.06 mg/l) had a persistent influence on the expressions of the transcripts, while the effect was transient under the lowest concentration (5.01 mg/l). It is suggested that the concentration under 5.01 mg/l might be safe for the development ofM. nipponense, but exceeding 12.06 mg/l may be harmful. When exposed to clean fresh water without BPA, the gene expressions rebounded a little. This may indicate that the biological damage of BPA was partly reversible after the prawns had been placed in the fresh water without BPA. So the BPA pollutant concentration should be controlled at secure levels in order to ensure safety in aquaculture, in this respect. This study provides fundamental data for the relationship between BPA and precocious maturation of the prawn, and will most probably contribute to the understanding of the sexual maturation process in crustaceans.

scholarly journals Tissue-expression profiles unveils the gene interaction of hepatopancreas, eyestalk, and ovary in precocious female Chinese mitten crab, Eriocheir sinensis

2018 ◽  
Author(s):  
Xiaowen Chen ◽  
Jun Wang ◽  
Xin Hou ◽  
Wucheng Yue ◽  
Shu Huang ◽  
...  
Keyword(s):  
Genetic Basis ◽  
Expression Profiles ◽  
Tissue Expression ◽  
Eriocheir Sinensis ◽  
Lipid Transport ◽  
Vitelline Membrane ◽  
Ovary Development ◽  
Chinese Mitten Crab ◽  
Mitten Crab ◽  
Neuropeptide Hormone

AbstractSexual precocity is a serious and common biological phenomenon in animal species. Large amount of precocity individuals was identified in Chinese mitten crab, Eriocheir sinensis, which caused huge economical loss every year. However, the underlying genetic basis of precocity in E. sinensis is still lack. In this study, histology observation, comparative transcriptome was conducted among different stages of precocious one-year old and normal two-year old E. sinensis, tissue-expression profiles of ovary, hepatopancreas, and eyestalk tissues were presented and compared. Genes associated with lipid metabolic process, lipid transport, vitelline membrane formation, vitelline synthesis and neuropeptide hormone related genes were upregulated in the ovary, hepatopancreas and eyestalk of precocious E. sinensis. Our results indicated eyestalk involved in neuroendocrine system providing neuropeptide hormone that may induce vitellogenesis in hepatopancreas and further stimulate ovary development. Hepatopancreas is a site for energy storage, vitellogenin synthesis and may assist to induce oogenesis through lipid transport in precocious E. sinensis. The genetic basis of precocity in E. sinensis is an integrated gene regulatory network of eyestalk, hepatopancreas, and ovary tissues. Our study provides effective convenient phenotype measurement method for identification of potential precocious E. sinensis detection, and valuable genetic resources and novel insights into the research of molecular mechanism of precocity in E. sinensis.

Sign in / Sign up

Export Citation Format

Share Document