Sulfur-starvation-induced Desulfurization Mechanism of Pseudomonas delafieldii R-8*

2010 ◽  
Vol 2009 (2) ◽  
pp. 235-239
Author(s):  
Zhijian YU ◽  
Huanjie LI ◽  
Haisheng WANG ◽  
Yuguang LI ◽  
Yanchun YAN ◽  
...  
Keyword(s):  
Sulfur Starvation ◽  
Pseudomonas Delafieldii

scholarly journals The Recovery from Sulfur Starvation is Independent from the mRNA Degradation Initiation Enzyme PARN in Arabidopsis

Plants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 380 ◽  
Author(s):  
Armbruster ◽  
Uslu ◽  
Wirtz ◽  
Hell
Keyword(s):  
Stress Responses ◽  
Splice Variants ◽  
Marker Genes ◽  
Sulfate Assimilation ◽  
Sulfur Deficiency ◽  
Protective Measures ◽  
Sulfur Starvation ◽  
Sulfate Assimilation Pathway ◽  
Growth Promoting

When plants are exposed to sulfur limitation, they upregulate the sulfate assimilation pathway at the expense of growth-promoting measures. Upon cessation of the stress, however, protective measures are deactivated, and growth is restored. In accordance with these findings, transcripts of sulfur-deficiency marker genes are rapidly degraded when starved plants are resupplied with sulfur. Yet it remains unclear which enzymes are responsible for the degradation of transcripts during the recovery from starvation. In eukaryotes, mRNA decay is often initiated by the cleavage of poly(A) tails via deadenylases. As mutations in the poly(A) ribonuclease PARN have been linked to altered abiotic stress responses in Arabidopsis thaliana, we investigated the role of PARN in the recovery from sulfur starvation. Despite the presence of putative PARN-recruiting AU-rich elements in sulfur-responsive transcripts, sulfur-depleted PARN hypomorphic mutants were able to reset their transcriptome to pre-starvation conditions just as readily as wildtype plants. Currently, the subcellular localization of PARN is disputed, with studies reporting both nuclear and cytosolic localization. We detected PARN in cytoplasmic speckles and reconciled the diverging views in literature by identifying two PARN splice variants whose predicted localization is in agreement with those observations.

Desulfurization of Model Oil Containing Dibenzothiophene or 4,6-Dimethyl Dibenzothiophene by Alginate Immobilized Cells

2011 ◽  
Vol 233-235 ◽  
pp. 1171-1176
Author(s):  
Ming Fang Luo ◽  
Hui Zhou Liu
Keyword(s):  
Immobilized Cells ◽  
Resting Cells ◽  
Negative Bacterium ◽  
Simple Method ◽  
Alginate Gel ◽  
The Stability ◽  
Model Oil ◽  
Pseudomonas Delafieldii ◽  
Ca Alginate ◽  
Desulfurization Activity

Gram-negative bacterium, Pseudomonas delafieldii R-8 (CGMCC 0570) is capable of desulfurizing dibenzothiophene (DBT) and 4,6-dimethyldibenzothiophene (4,6-DMDBT) to produce corresponding monohydroxydimethylbiphenyl. The immobilization of the resting cells of this strain in Ca-alginate gel effectively improved the stability of the cells and the desulfurization ability per amount of cells. 1 mmol/L of DBT and 4,6-DMDBT could be completely degraded in about 1 d. About 39 percent of the activity for 4,6-DMDBT was recovered after immobilization. The desulfurization activity was increased with the decrease of the diameter of the beads. The Ca-alginate immobilized cells could be used repeatedly for over 190 h with the addition of calcium ions to strengthen them. A thin layer of hydrophobic polyurea was coated on the surface of Ca-alginate gel using a simple method. The desulfurization activity was enhanced after the coating.

scholarly journals Simultaneous Removal of Thiophene and Dibenzothiophene by Immobilized Pseudomonas delafieldii R-8 cells

2012 ◽  
Vol 20 (1) ◽  
pp. 47-51 ◽  
Author(s):  
Huang TANG ◽  
Qiang LI ◽  
Zelong WANG ◽  
Daojiang YAN ◽  
Jianmin XING
Keyword(s):  
Simultaneous Removal ◽  
Pseudomonas Delafieldii

scholarly journals Global Regulation of the Response to Sulfur Availability in the Cheese-Related BacteriumBrevibacterium aurantiacum

2010 ◽  
Vol 77 (4) ◽  
pp. 1449-1459 ◽  
Author(s):  
Marie-Pierre Forquin ◽  
Agnès Hébert ◽  
Aurélie Roux ◽  
Julie Aubert ◽  
Caroline Proux ◽  
...  
Keyword(s):  
Expression Profiles ◽  
Sulfur Metabolism ◽  
Metabolic Reconstruction ◽  
Sulfate Assimilation ◽  
Sulfur Starvation ◽  
Expression Of Genes ◽  
Transsulfuration Pathway ◽  
Sulfate Assimilation Pathway ◽  
Genes Encoding ◽  
Sulfur Containing

ABSTRACTIn this study, we combined metabolic reconstruction, growth assays, and metabolome and transcriptome analyses to obtain a global view of the sulfur metabolic network and of the response to sulfur availability inBrevibacterium aurantiacum. In agreement with the growth ofB. aurantiacumin the presence of sulfate and cystine, the metabolic reconstruction showed the presence of a sulfate assimilation pathway, thiolation pathways that produce cysteine (cysEandcysK) or homocysteine (metXandmetY) from sulfide, at least one gene of the transsulfuration pathway (aecD), and genes encoding three MetE-type methionine synthases. We also compared the expression profiles ofB. aurantiacumATCC 9175 during sulfur starvation or in the presence of sulfate. Under sulfur starvation, 690 genes, including 21 genes involved in sulfur metabolism and 29 genes encoding amino acids and peptide transporters, were differentially expressed. We also investigated changes in pools of sulfur-containing metabolites and in expression profiles after growth in the presence of sulfate, cystine, or methionine plus cystine. The expression of genes involved in sulfate assimilation and cysteine synthesis was repressed in the presence of cystine, whereas the expression ofmetX,metY,metE1,metE2, andBL613, encoding a probable cystathionine-γ-synthase, decreased in the presence of methionine. We identified three ABC transporters: two operons encoding transporters were transcribed more strongly during cysteine limitation, and one was transcribed more strongly during methionine depletion. Finally, the expression of genes encoding a methionine γ-lyase (BL929) and a methionine transporter (metPS) was induced in the presence of methionine in conjunction with a significant increase in volatile sulfur compound production.

scholarly journals Nitrogen or Sulfur Starvation Differentially Affects Phycobilisome Degradation and Expression of the nblA Gene in Synechocystis Strain PCC 6803

2001 ◽  
Vol 183 (10) ◽  
pp. 2989-2994 ◽  
Author(s):  
Catherine Richaud ◽  
Gérald Zabulon ◽  
Annette Joder ◽  
Jean-Claude Thomas
Keyword(s):  
Sequence Data ◽  
Open Reading Frames ◽  
Northern Hybridization ◽  
Sulfur Starvation ◽  
P Limitation ◽  
Kanamycin Cassette ◽  
N Starvation ◽  
Pcc 7942 ◽  
Reading Frames ◽  
Pcc 6803

ABSTRACT Nitrogen (N) limitation in cyanobacteria is well documented: a reduced growth rate is observed, accompanied by a cessation of phycobiliprotein synthesis and an ordered degradation of phycobilisomes (PBS). This leads to a dramatic bleaching phenomenon known as chlorosis. In Synechococcus strain PCC 7942, bleaching due to PBS degradation is also observed under sulfur (S) or phosphorus (P) limitation, and all three are under the control of the nblAgene product, a 59-amino-acid polypeptide which is overexpressed under N, S, and P starvation (J. L. Collier, and A. R. Grossman, EMBO J. 13:1039–1047, 1994). Cyanobase sequence data forSynechocystis strain PCC 6803 indicate the presence of two tandem open reading frames (sll0452 and sll0453) homologous tonblA. We cloned the two genes, identified a unique 5′ mRNA end suggestive of a single transcription start site, and studiednblA expression under conditions of N or S starvation by Northern hybridization: transcripts were detected only under N starvation (no signal is detected in replete medium or with S starvation), whether nblA1 or nblA2 was used as a probe. Mutations in nblA1 and nblA2 were constructed by insertion of a kanamycin cassette; both mutations were nonbleaching under N starvation. Synechocystis strain PCC 6803 does not bleach under S starvation, consistent with the absence ofnblA induction in these conditions. These results were confirmed by analysis of the PBS components: sequential degradation of phycocyanin and associated linkers was observed only under conditions of N starvation. This indicates differences betweenSynechocystis strain PCC 6803 and Synechococcusstrain PCC 7942 in their regulatory and signaling pathways leading to N- and S-starved phenotypes.

scholarly journals A Single Monooxygenase, Ese, Is Involved in the Metabolism of the Organochlorides Endosulfan and Endosulfate in an Arthrobacter sp

2006 ◽  
Vol 72 (5) ◽  
pp. 3524-3530 ◽  
Author(s):  
Kahli M. Weir ◽  
Tara D. Sutherland ◽  
Irene Horne ◽  
Robyn J. Russell ◽  
John G. Oakeshott
Keyword(s):  
Sole Source ◽  
Amino Acid Identity ◽  
Open Reading Frames ◽  
Sulfur Source ◽  
Toxic Metabolite ◽  
16S Rrna Sequence ◽  
Soil Microbial ◽  
Sulfur Starvation ◽  
Soil Microbial Population ◽  
Reading Frames

ABSTRACT In this paper we describe isolation of a bacterium capable of degrading both isomers of the organochloride insecticide endosulfan and its toxic metabolite, endosulfate. The bacterium was isolated from a soil microbial population that was enriched with continuous pressure to use endosulfate as the sole source of sulfur. Analysis of the 16S rRNA sequence of the bacterium indicated that it was an Arthrobacter species. The organochloride-degrading activity was not observed in the presence of sodium sulfite as an alternative sulfur source, suggesting that the activity was part of the sulfur starvation response of the strain. A gene, ese, encoding an enzyme capable of degrading both isomers of endosulfan and endosulfate was isolated from this bacterium. The enzyme belongs to the two-component flavin-dependent monooxygenase family whose members require reduced flavin for activity. Nuclear magnetic resonance analyses identified the metabolite of endosulfan as endosulfan monoalcohol and the metabolite of endosulfate as endosulfan hemisulfate. The ese gene was located in a cluster of 10 open reading frames encoding proteins with low levels of sulfur-containing amino acids. These open reading frames were organized into two apparent divergently orientated operons and a gene encoding a putative LysR-type transcriptional regulator. The operon not containing ese did contain a homologue whose product exhibited 62% amino acid identity to the ese-encoded protein.

scholarly journals A global response to sulfur starvation inPseudomonas putidaand its relationship to the expression of low-sulfur-content proteins

2007 ◽  
Vol 267 (2) ◽  
pp. 184-193 ◽  
Author(s):  
Colin Scott ◽  
Margaret E. Hilton ◽  
Christopher W. Coppin ◽  
Robyn J. Russell ◽  
John G. Oakeshott ◽  
...  
Keyword(s):  
Sulfur Content ◽  
Global Response ◽  
Sulfur Starvation ◽  
Low Sulfur Content ◽  
Low Sulfur

scholarly journals Transcriptional and Proteomic Profiling of Aspergillus flavipes in Response to Sulfur Starvation

PLoS ONE ◽  
2015 ◽  
Vol 10 (12) ◽  
pp. e0144304 ◽  
Author(s):  
Ashraf S. A. El-Sayed ◽  
Marwa A. Yassin ◽  
Gul Shad Ali
Keyword(s):  
Proteomic Profiling ◽  
Sulfur Starvation ◽  
Aspergillus Flavipes
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