scholarly journals Differential Gene Expression in Human Glioma Cells: Correlation With Presence or Absence of DNA-Dependent Protein Kinase

2003 ◽  
Vol 11 (1) ◽  
pp. 35-46 ◽  
Author(s):  
RONG AI ◽  
ANA SANDOVAL ◽  
PAUL LABHART
Keyword(s):  
Gene Expression ◽  
Protein Kinase ◽  
Differential Gene Expression ◽  
Glioma Cells ◽  
Human Glioma ◽  
Human Glioma Cells ◽  
Dependent Protein Kinase ◽  
Dependent Protein ◽  
Differential Gene

cDNA Cloning and Gene Expression of Human Type Iα cGMP-Dependent Protein Kinase

Hypertension ◽  
1996 ◽  
Vol 27 (3) ◽  
pp. 552-557 ◽  
Author(s):  
Naohisa Tamura ◽  
Hiroshi Itoh ◽  
Yoshihiro Ogawa ◽  
Osamu Nakagawa ◽  
Masaki Harada ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Kinase ◽  
Cdna Cloning ◽  
Dependent Protein Kinase ◽  
Human Type ◽  
Cgmp Dependent Protein Kinase ◽  
Dependent Protein

scholarly journals Chronic Elevation of Calmodulin in the Ventricles of Transgenic Mice Increases the Autonomous Activity of Calmodulin-Dependent Protein Kinase II, Which Regulates Atrial Natriuretic Factor Gene Expression

2000 ◽  
Vol 14 (8) ◽  
pp. 1125-1136 ◽  
Author(s):  
Josep M. Colomer ◽  
Anthony R. Means
Keyword(s):  
Gene Expression ◽  
Protein Kinase ◽  
Transgenic Mice ◽  
Atrial Natriuretic Factor ◽  
Ventricular Myocytes ◽  
Dependent Protein Kinase ◽  
Natriuretic Factor ◽  
Dependent Protein ◽  
Autonomous Activity

Abstract Although isoforms of Ca2+/calmodulin-dependent protein kinase II (CaMKII) have been implicated in the regulation of gene expression in cultured cells, this issue has yet to be addressed in vivo. We report that the overexpression of calmodulin in ventricular myocytes of transgenic mice results in an increase in the Ca2+/calmodulin-independent activity of endogenous CaMKII. The calmodulin transgene is regulated by a 500-bp fragment of the atrial natriuretic factor (ANF) gene promoter which, based on cell transfection studies, is itself known to be regulated by CaMKII. The increased autonomous activity of CaMKII maintains the activity of the transgene and establishes a positive feedforward loop, which also extends the temporal expression of the endogenous ANF promoter in ventricular myocytes. Both the increased activity of CaMKII and transcriptional activation of ANF are highly selective responses to the chronic overexpression of calmodulin. These results indicate that CaMKII can regulate gene expression in vivo and suggest that this enzyme may represent the Ca2+-dependent target responsible for reactivation of the ANF gene during ventricular hypertrophy.

scholarly journals Cysteine-rich 61-associated gene expression profile alterations in human glioma cells

2017 ◽  
Vol 16 (4) ◽  
pp. 5561-5567
Author(s):  
Rui Wang ◽  
Bo Wei ◽  
Jun Wei ◽  
Yu Tian ◽  
Chao Du
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Glioma Cells ◽  
Human Glioma ◽  
Human Glioma Cells

scholarly journals Opiates and cultured neuroblastoma X glioma cells. Effect on cyclic AMP and polyamine levels and on ornithine decarboxylase and protein kinase activities

1985 ◽  
Vol 227 (2) ◽  
pp. 389-395 ◽  
Author(s):  
D Benalal ◽  
U Bachrach
Keyword(s):  
Protein Kinase ◽  
Cyclic Amp ◽  
Drug Addiction ◽  
Ornithine Decarboxylase ◽  
Normal Values ◽  
Biological Activities ◽  
Glioma Cells ◽  
Acute Exposure ◽  
Dependent Protein Kinase ◽  
Dependent Protein

In cultured NG 108-15 neuroblastoma x glioma cells, opiates decreased cellular cyclic AMP and polyamine levels. This decrease was related to the inhibition of ornithine decarboxylase and cyclic AMP-dependent protein kinase activities during the acute exposure of the cells to the drugs. Growing the cells in the presence of opiates for several days led to drug addiction. In the tolerant-addicted cells, polyamine and cyclic AMP levels were close to normal values as were the activities of ornithine decarboxylase and cyclic AMP-dependent protein kinase. Removal of the opiate from ‘addicted’ cells, by either washing or by adding the antagonist naloxone, resulted in an increase in cyclic AMP and polyamine levels and the activities of ornithine decarboxylase and cyclic AMP-dependent protein kinase. The effect of opiates was closely related to their biological activities. Inactive enantiomorphs did not affect cyclic AMP or polyamine levels; neither did they decrease ornithine decarboxylase and cyclic AMP-dependent protein kinase activities.

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