Inhibition of 5-Fluorouracil Activation by Oxipurinol in Preparations of Normal Rat Tissues and Jensen Sarcoma: Tumor-Selective Stimulation by Inosine

1991 ◽  
Vol 3 (9) ◽  
pp. 271-276
Author(s):  
Richard E. Beltz ◽  
LeAnn L. Poland
Keyword(s):  
Rat Tissues ◽  
Selective Stimulation ◽  
Normal Rat ◽  
Tumor Selective

Expression of vascular permeability factor/vascular endothelial growth factor in normal rat tissues

1993 ◽  
Vol 264 (4) ◽  
pp. C995-C1002 ◽  
Author(s):  
W. T. Monacci ◽  
M. J. Merrill ◽  
E. H. Oldfield
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
In Situ Hybridization ◽  
Rat Tissues ◽  
Vascular Endothelial ◽  
Vascular Permeability Factor ◽  
Organ Specific ◽  
Normal Rat ◽  
Endothelial Growth Factor ◽  
The Brain

Vascular permeability factor (VPF)/vascular endothelial growth factor (VEGF) is a approximately 43-kDa secreted protein that has been shown in bioassays to induce endothelial proliferation, angiogenesis, and capillary hyperpermeability. VPF has been suggested to play an important role in the physiology of normal vasculature. To further elucidate the natural functions of VPF in vivo, the expression of VPF in normal tissues was examined using Northern blot analysis and in situ hybridization histochemistry. VPF mRNA is expressed in the brain, kidney, liver, lung, and spleen of the healthy adult rat. On Northern blots, the relative abundance of VPF mRNA observed in these tissues was highest in the lung and lowest in the spleen. As determined by in situ hybridization, the patterns of VPF expression are organ specific. Hybridization of an antisense VPF probe was concentrated in the cerebellar granule cell layer of the brain and in the glomeruli and tubules of the kidney. In the liver and lung, intense hybridization was observed homogeneously throughout both tissues, demonstrating that VPF mRNA is present in virtually every hepatocyte and pulmonary alveolar cell. Hybridization to the spleen was weaker and more diffuse. The widespread expression and organ-specific distribution of VPF mRNA in normal rat tissues supports the suggestion of an extensive role for this factor in the physiology of normal vasculature.

scholarly journals The distribution of collagenase in normal rat tissues.

1975 ◽  
Vol 23 (12) ◽  
pp. 910-920 ◽  
Author(s):  
I Montfort ◽  
R Pérez-Tamayo
Keyword(s):  
Connective Tissue ◽  
Immunoglobulin G ◽  
Basement Membranes ◽  
Rat Tissues ◽  
Collagenase Activity ◽  
Rat Uterus ◽  
Triceps Muscle ◽  
Normal Rat ◽  
Immunohistochemical Methods ◽  
Tail Tendon

A rabbit monospecific anti-rat uterus collagenase antibody has been used to study the distribution of collagenase in normal rat tissues by immunohistochemical methods. Indirect staining was performed with fluorescein-conjugated goat anti-rabbit immunoglobulin G antibody. The organs studied were brain, lung, myocardium, liver, spleen, kidney, adrenal, testes, uterus, xiphoid cartilage, tail tendon, skeletal (triceps) muscle and skin. Collagenase is widely present throughout the connective tissue structures in all organs examined. The enzyme is apparently bound to collagen fibers, reticulum fibers and basement membranes. The results suggest that control of collagenase activity depends on factors other than the presence of the enzyme in tissues.

Distribution of basic azo-dye-binding protein in normal rat tissues and carcinogen-induced liver tumors

1973 ◽  
Vol 11 (2) ◽  
pp. 398-411 ◽  
Author(s):  
G. A. Bannikov ◽  
V. I. Guelstein ◽  
T. A. Tchipysheva
Keyword(s):  
Azo Dye ◽  
Liver Tumors ◽  
Binding Protein ◽  
Rat Tissues ◽  
Dye Binding ◽  
Normal Rat

scholarly journals Spexin Expression in Normal Rat Tissues

2010 ◽  
Vol 58 (9) ◽  
pp. 825-837 ◽  
Author(s):  
Andrea Porzionato ◽  
Marcin Rucinski ◽  
Veronica Macchi ◽  
Carla Stecco ◽  
Ludwik K. Malendowicz ◽  
...  
Keyword(s):  
Rat Tissues ◽  
Normal Rat

Acth Immunoactivity in Normal rat Tissues: Modulation by Hypophysectomy and Adrenalectomy

1992 ◽  
Vol 18 (1) ◽  
pp. 59-75 ◽  
Author(s):  
Campbell V. Kyle ◽  
William D. Odell
Keyword(s):  
Rat Tissues ◽  
Normal Rat

scholarly journals THE HISTOCHEMICAL DEMONSTRATION OF NUCLEASE ACTIVITY WITH FILMS OF SOLUBLE RIBONUCLEIC ACID AND POLYADENYLIC ACID

1972 ◽  
Vol 20 (5) ◽  
pp. 350-357 ◽  
Author(s):  
R. DAOUST ◽  
R. MORAIS
Keyword(s):  
Enzyme Activity ◽  
Ribonucleic Acid ◽  
Nuclease Activity ◽  
Histochemical Demonstration ◽  
The Other ◽  
Rat Tissues ◽  
Other Hand ◽  
Polyadenylic Acid ◽  
Normal Rat

Films of soluble ribonucleic acid (sRNA) and polyadenylic acid (poly-A) were used to investigate the distribution of nuclease activity in normal rat tissues. The reactions obtained with films of sRNA were similar to those previously observed with standard RNA and both substrates apparently reveal the same group of nucleases. On the other hand, the distribution of enzyme activity shown by films of poly-A differed markedly from that observed with RNA films, and it appears that films of poly-A demonstrate a different group of nucleases.

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