scholarly journals Detection of oral spirochete (Treponema denticola) by modified Fontana staining technique from sub-gingival plaques of patients suffering from periodontal disease and to analyse the morphology of spirochetes with respect to the disease’s status

2020 ◽  
Vol 6 (8) ◽  
pp. 286
Author(s):  
Sunil Paudel ◽  
Jyoti M. Nagamoti ◽  
Ved Prakash Mishra ◽  
Suraj Twanabasu ◽  
Susan Kusma
Keyword(s):  
Periodontal Disease ◽  
Screening Method ◽  
Staining Technique ◽  
Laboratory Diagnosis ◽  
Global Scale ◽  
Personal Hygiene ◽  
Treponema Denticola ◽  
Periodontal Pathogens ◽  
Periodontal Tissues ◽  
Periodontal Infection

<p class="abstract"><strong>Background:</strong> Infections pertaining to mankind particularly those affecting the periodontal tissues are of serious concerns worldwide and are increasing on a global scale particularly in the tropical and subtropical countries like India. The clinical presentation, though very typical of periodontal infection, is often confused with other oral infection, making laboratory diagnosis and confirmation necessary. The management of periodontal infections needs personal hygiene, awareness of infection, proper diagnosis and medication. The present study was undertaken to demonstrate the oral spirochete (<em>Treponema denticola</em>) from periodontal pathogens suffering from periodontal disease.</p><p class="abstract"><strong>Methods:</strong> A total of 55 clinically diagnosed selected patients of periodontal infection attending the outpatient Department of Periodontics, V. K. Institute of Dental Science, KLE University, Belgaum were studied. Dental plaque was taken as specimens from the patients were processed by modified Fontana staining and observed in microscope.  </p><p class="abstract"><strong>Results:</strong> A total number of 55 plaque samples were stained by modified Fontana staining technique. 30 cases were positive and 25 cases were negative. Males were affected more as compared to the females and the age group ranging from 15 to 65. Farmers were more affected compared to other occupation.</p><p class="abstract"><strong>Conclusions:</strong> Microscopic method by modified silver nitrate staining can be a very useful screening method for evaluation of oral spirochetes in a clinical setting if used judiciously keeping in mind the variables that can affect the results.</p>

Comparison of Sampling Techniques For qPCR Quantification of Periodontal Pathogens

2018 ◽  
Vol 68 (12) ◽  
pp. 2853-2856 ◽  
Author(s):  
Igor Jelihovschi ◽  
Cristian Drochioi ◽  
Aida Corina Badescu ◽  
Raoul Vasile Lupusoru ◽  
Alexandra Elena Munteanu ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Periodontal Diseases ◽  
Pcr Analysis ◽  
Treponema Denticola ◽  
Subgingival Plaque ◽  
Periodontal Pathogens ◽  
Radiographic Evidence ◽  
Gingival Bleeding ◽  
As Species ◽  
Qpcr Quantification

The diagnosis of periodontal disease is mainly based on use of clinical and radiographic evidence. In this study we employed a quantitative PCR analysis of Aggregatibacter actinomycetemcomitans and Treponema denticola as species strongly involved in periodontal diseases, burden in periodontal pockets to detect the main sampling factors that interfere with qPCR results. From 22 patients with advanced periodontal disease, subgingival plaque was comparatively collected by paper points and periodontal Gracey curettes. Samples were collected from the same situs in presence of gingival bleeding and absence of bleeding. The concordance and agreement of results between samples were assessed. The present study demonstrates that subgingival plaque sampling with sterile absorbable paper points is often accompanied by gingival bleeding resulting in quantification biases of periodontal pathogens.

scholarly journals Invasion of Oral and Aortic Tissues by Oral Spirochete Treponema denticola in ApoE−/−Mice Causally Links Periodontal Disease and Atherosclerosis

2014 ◽  
Vol 82 (5) ◽  
pp. 1959-1967 ◽  
Author(s):  
Sasanka S. Chukkapalli ◽  
Mercedes F. Rivera ◽  
Irina M. Velsko ◽  
Ju-Youn Lee ◽  
Hao Chen ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Alveolar Bone ◽  
Tissue Growth ◽  
Aortic Tissue ◽  
Treponema Denticola ◽  
Rapid Progression ◽  
Causative Role ◽  
Igg Antibody ◽  
Content Type ◽  
Periodontal Infection

ABSTRACTTreponema denticolais a predominantly subgingival oral spirochete closely associated with periodontal disease and has been detected in atherosclerosis. This study was designed to evaluate causative links between periodontal disease induced by chronic oralT. denticolainfection and atherosclerosis in hyperlipidemic ApoE−/−mice. ApoE−/−mice (n= 24) were orally infected withT. denticolaATCC 35404 and were euthanized after 12 and 24 weeks.T. denticolagenomic DNA was detected in oral plaque samples, indicating colonization of the oral cavity. Infection elicited significantly (P= 0.0172) higher IgG antibody levels and enhanced intrabony defects than sham infection.T. denticola-infected mice had higher levels of horizontal alveolar bone resorption than sham-infected mice and an associated significant increase in aortic plaque area (P≤ 0.05). Increased atherosclerotic plaque correlated with reduced serum nitric oxide (NO) levels and increased serum-oxidized low-density lipoprotein (LDL) levels compared to those of sham-infected mice.T. denticolainfection altered the expression of genes known to be involved in atherosclerotic development, including the leukocyte/endothelial cell adhesion gene (Thbs4), the connective tissue growth factor gene (Ctgf), and the selectin-E gene (Sele). Fluorescentin situhybridization (FISH) revealedT. denticolaclusters in both gingival and aortic tissue of infected mice. This is the first study examining the potential causative role of chronicT. denticolaperiodontal infection and vascular atherosclerosisin vivoin hyperlipidemic ApoE−/−mice.T. denticolais closely associated with periodontal disease and the rapid progression of atheroma in ApoE−/−mice. These studies confirm a causal link for active oralT. denticolainfection with both atheroma and periodontal disease.

scholarly journals MICRO BIOLOGY OF PERIODONTAL DISEASE- A REVIEW

2021 ◽  
Vol 5 (6) ◽  
Author(s):  
Jageer Chinna ◽  
Jannat Sharma
Keyword(s):  
Periodontal Disease ◽  
Periodontal Diseases ◽  
Disease Process ◽  
Fusobacterium Nucleatum ◽  
Host Cells ◽  
Bacterial Invasion ◽  
Periodontal Pathogens ◽  
Tissue Destruction ◽  
Campylobacter Rectus ◽  
Periodontal Tissues

Periodontal diseases are inflammatory and destructive diseases of the dentogingival complex associated with specific periodontal pathogens inhabiting periodontal pockets. Periodontal diseases lead to damage of the periodontal tissues supporting the teeth (bone and connective tissue) and affect the quality of life of the affected individuals: poor alimentation, tooth loss, social and financial problems. Although it is generally considered that the disease has multifactorial etiology, data show that some specific Gram-negative microorganisms in the subgingival plaque biofilm play a major role in the initiation and progression of periodontitis. Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia form a consortium in the subgingival biofilm and are regarded as the principal periodontopathogenic bacteria. Other microorganisms that have been implicated as predominant species in the disease process are: Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Prevotella intermedia, Campylobacter rectus, Peptostreptococcus migros, Eikenella corrodens. In periodontitis, the initiation of the disease is the colonization of the tissues by these pathogenic species. The next step is bacterial invasion or invasion by pathogenic products into the periodontal tissues, interactions of bacteria or their substances with host cells, and this directly/indirectly causes degradation of the periodontium, resulting in tissue destruction. Keywords: periodontal disease, periodontal pathogens, microbiology.

A Study to Evaluate Awareness of Psychosomatic Factors as a Possible Risk Factor for Periodontal Diseases in General Dental Practitioners of Mangalore City

2011 ◽  
Vol 5 (1) ◽  
pp. 19-23
Author(s):  
Dilip G Nayak ◽  
Sangeeta Umesh Nayak ◽  
Ashita Uppoor ◽  
Keshava Pai
Keyword(s):  
Periodontal Disease ◽  
Psychological Factors ◽  
Periodontal Diseases ◽  
Primary Care Providers ◽  
Epidemiological Studies ◽  
Care Providers ◽  
Periodontal Tissues ◽  
Periodontal Infection ◽  
Dental Practitioners ◽  
As Stress

ABSTRACT Background Clinical observations and epidemiological studies suggest that some negative life events and psychological factors may contribute to an increased susceptibility to periodontal diseases. Management of periodontal diseases is a fundamental part of general practice. This study explored general dental practitioner's awareness about psychosomatic factors and their role in periodontal disease progression. Aim To evaluate the awareness of general dental practioners of Mangalore city and their professional behavior towards patients with stress and depression and periodontal infection. Method Data were collected from 55 general dental practitioners of Mangalore city. 27 respondents were purely general practitioners and other were academicians who were practicing general dentistry. Results the respondents had awareness about the effect of stress and depression on periodontal tissues and their management. They were able to identify the periodontal conditions which occur primarily because of stress. Conclusion The general dental practitioners play a key role in the success of the treatment of periodontal disease since they are the primary care providers. The respondents were significantly aware about the psychological factors such as stress and depression and its significance on periodontium. This may be due to the involvement of more number of young practitioners who were well educated about psychosomatic factors and their effects on periodontium in their dental education.

scholarly journals Porphyromonas gingivalisandTreponema denticolaMixed Microbial Infection in a Rat Model of Periodontal Disease

2010 ◽  
Vol 2010 ◽  
pp. 1-10 ◽  
Author(s):  
Raj K. Verma ◽  
Sunethra Rajapakse ◽  
Archana Meka ◽  
Clayton Hamrick ◽  
Sheela Pola ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Rat Model ◽  
Alveolar Bone ◽  
Treponema Denticola ◽  
Periodontal Pathogens ◽  
Microbial Infection ◽  
Morphometric Measurements ◽  
Th2 Immune Response ◽  
Microbial Infections ◽  
Antibody Levels

Porphyromonas gingivalisandTreponema denticolaare periodontal pathogens that express virulence factors associated with the pathogenesis of periodontitis. In this paper we tested the hypothesis thatP. gingivalisandT. denticolaare synergistic in terms of virulence; using a model of mixed microbial infection in rats. Groups of rats were orally infected with eitherP. gingivalisorT. denticolaor mixed microbial infections for 7 and 12 weeks.P. gingivalisgenomic DNA was detected more frequently by PCR thanT. denticola. Both bacteria induced significantly high IgG, IgG2b, IgG1, IgG2a antibody levels indicating a stimulation of Th1 and Th2 immune response. Radiographic and morphometric measurements demonstrated that rats infected with the mixed infection exhibited significantly more alveolar bone loss than shaminfected control rats. Histology revealed apical migration of junctional epithelium, rete ridge elongation, and crestal alveolar bone resorption; resembling periodontal disease lesion. These results showed thatP. gingivalisandT. denticolaexhibit no synergistic virulence in a rat model of periodontal disease.

scholarly journals Evaluation of Oral Hygiene in Patients with Generalized Periodontitis of II Degree and Stage II Hypertension

2016 ◽  
Vol 23 (4) ◽  
pp. 2016419
Author(s):  
Tetiana Vivcharenko ◽  
Mykola Rozhko
Keyword(s):  
Oral Cavity ◽  
Periodontal Disease ◽  
Oral Hygiene ◽  
Main Group ◽  
Stage Ii ◽  
Personal Hygiene ◽  
Control Group ◽  
Poor Oral Hygiene ◽  
Periodontal Tissues ◽  
The Status

Generalized periodontitis is the most common form of periodontal pathology, especially in the second half of life. Nowadays, the problem of periodontal disease is relevant due to its high prevalence, tendency to progression, multifaceted influence on the dentoalveolar system and the whole organism as well as uncertain treatment. Therefore, there is a need to find optimal ways of prevention and treatment of this disease. Close relationships between periodontal pathology and systemic diseases, such as hypertension, which affects every 2nd-3rd adult were determined.The objective of the research was to determine the status of oral cavity hygiene in patients with generalized periodontitis of II degree and stage II hypertension.Materials and methods. The study included 30 patients with generalized periodontitis of II degree and stage II hypertension being treated in the Ivano-Frankivsk Regional Clinical Cardiology Dispensary; the average age ranged from 35 to 54 years (the main group). The control group included 10 patients of the same age without generalized periodontitis and somatic pathology .The status of oral cavity hygiene was determined using the Green Vermillion index. The diagnosis of periodontal disease was made on the basis of the classification proposed by M.F. Danilevskyi. The obtained results were subjected to variation-statistical analysis using statistical package “Stat Soft 6.0”; classical methods of variational statistics were applied; mean values and their reliability were evaluated.Results. The results of examination showed poor oral hygiene in almost all patients. The analysis of hygienic indices showed the following results: in patients of the main group, the Green Vermillion index was 1.99 ± 0.13 points (p<0.001) which corresponds to unsatisfactory level of oral hygiene. In patients of the control group, this index was 1.10 ± 0.17 points which corresponds to satisfactory level of oral hygiene. According to the index, 9 (30.0%) patients of the main group had satisfactory oral hygiene, 12 (40.0%) patients had poor oral hygiene, and in 9 (30.0%) patients, poor oral hygiene was observed.In the control group, 2 (20.0%) patients had good oral hygiene, 6 (60.0%) patients had satisfactory oral hygiene and in 2 (20.0%) patients, unsatisfactory oral hygiene was observed. There were no patients with poor oral hygiene. The analysis of the indicators of the Green Vermillion index showed that in case of the pathological process exacerbation the oral hygiene status in patients deteriorated.Conclusions. The level of oral hygiene in patients of both groups was low due to incorrect selection of personal hygiene products or their untimely replacement. In patients with generalized periodontitis of II degree and stage II hypertension, the level of oral hygiene was lower than in somatically healthy persons: the worse status of oral cavity hygiene – the more pronounced changes in the periodontal tissues. We can suppose that high blood pressure affects the status of the oral cavity, creates a higher risk and exacerbates the periodontal diseases.

scholarly journals INVESTIGATION OF SPECIES COMPOSITION OF MICROFLORA IN PERIODONTAL POCKETS IN PATIENTS WITH GENERALIZED PERIODONTITIS AND METABOLIC SYNDROME

2021 ◽  
Vol 21 (3) ◽  
pp. 147-150
Author(s):  
T.A. Hlushchenko
Keyword(s):  
Metabolic Syndrome ◽  
Periodontal Disease ◽  
Species Composition ◽  
Periodontal Diseases ◽  
Oral Microbiome ◽  
Periodontal Pathogens ◽  
Periodontal Tissues ◽  
The Metabolic Syndrome ◽  
Microbial Invasion ◽  
Rank One

Among dental diseases, periodontal diseases rank one of the leading places and are considered as the most pressing issues of modern dentistry. The presence of concomitant somatic pathology, in particular, cardiovascular, endocrinological, autoimmune diseases is an important factor that considerably determines the course and prognosis of periodontal disease. Metabolic syndrome is regarded as an urgent social and medical issue due to its high prevalence among the general population and its contribution to the development and progression of cardiovascular disease. The number of reports and scientific interest in the metabolic syndrome has grown up significantly in recent years, but despite the significant number of studies, the oral microbiome in patients with periodontal disease and underlying metabolic syndrome is still remaining insufficiently studied. The aim of this work was to investigate the species composition of the microflora in periodontal pockets and the frequency of excretion of certain types of microorganisms in the acute generalized periodontitis in patients with metabolic syndrome. A microbiological study was performed in 30 people with metabolic syndrome and generalized periodontitis, who formed the main group, and in 30 people with generalized periodontitis without endocrinological pathology, who formed a comparison group. The results of microbiological examination indicate pathological changes in the oral microbiome in the patients with metabolic syndrome demonstrating a predominance of periodontal pathogens. It can be assumed that the components of the metabolic syndrome can initiate and support microbial invasion thus resulting in the inflammatory reaction of periodontal tissues. There is a similarity between pathogenetic mechanisms of metabolic syndrome and periodontal disease that lead to the impairment of all types of metabolism: protein, lipid, mineral, carbohydrate. As a consequence, this contributes to the progressive destruction of oral tissues. The obtained data enable to suggest the dependence between the presence of the patient's metabolic syndrome and the development of intensive damage to periodontal tissues.

Abstract TP85: Impact of Periodontal Disease and -Bacteria on Intracranial Aneurysms

Stroke ◽  
2016 ◽  
Vol 47 (suppl_1) ◽  
Author(s):  
Shotaro Yoshioka
Keyword(s):  
Periodontal Disease ◽  
Intracranial Aneurysms ◽  
Inflammatory Responses ◽  
Periodontal Diseases ◽  
Gram Negative Bacteria ◽  
Periodontal Pathogens ◽  
Treatment Needs ◽  
Gram Negative ◽  
Periodontal Tissues ◽  
Periodontal Index

Introduction: Periodontal Gram-negative bacteria and their products can initiate inflammatory responses in periodontal tissues with systemic consequences. They are associated with the pathogenesis of atherosclerosis and ischemic stroke. Local inflammation and oxidative stress play a crucial role in the pathophysiology of intracranial aneurysms (IAs). Under the hypothesis that the severity of periodontal disease is associated with the formation and rupture of IAs we assessed which periodontal pathogens contribute to the pathogenesis of IAs. Methods: We enrolled patients with ruptured- (n=5, age 60±11.9) and unruptured IAs (n=13, age 67±6.1) and controls without IAs (n=7, age 58±8.5); their prior informed consent was obtained. The severity of periodontitis was recorded using the community periodontal index (CPI) of the Treatment Needs code. Subgingival plaques (n=23) were evaluated with the quantitative real-time PCR assay to check for the Gram-negative bacteria Aggregatibacter actinomycetemcomitans (Aa), Fusobacterium nucleatum, Treponema denticola, Prevotella intermedia (Pi), Tannerella forsythia, and Porphyromonas gingivalis (Pg). Plasma IgG titers of antibody against Pg, Pi, Aa, and Eikenella corrodens were evaluated by ELISA. Results: The CPI was significantly higher in patients with IAs than the controls (2.7 vs 1.9, p<0.05) and their DNA level of subgingival plaques and their plasma IgG titers of Pg were also higher. Periodontal disease was more severe and the plasma IgG titers of Pg were higher in patients with ruptured- than unruptured IAs, suggesting that Pg is associated not only with the formation but also the rupture of IAs. Conclusions: We present evidence that severe periodontal disease and Pg infection may be involved in the pathophysiology of IAs. The management of periodontal diseases may help to prevent the formation and rupture of IAs.

scholarly journals Review- The periodontal pathogen Treponema denticola: an atherosclerosis risk factor

2021 ◽  
Vol 10 (1) ◽  
pp. e25810111637
Author(s):  
Pâmela Beatriz do Rosário Estevam dos Santos ◽  
Patrícia Michelle Nagai de Lima ◽  
Ana Luiza do Rosário Palma ◽  
Amjad Abu Hasna ◽  
Rodnei Dennis Rossoni ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Periodontal Diseases ◽  
Systemic Disease ◽  
Periodontal Pathogen ◽  
In Vivo Studies ◽  
Treponema Denticola ◽  
Periodontal Pathogens ◽  
Atherosclerosis Risk Factor

Objective: Treponema denticola “T. denticola” is a pathogen associated with periodontal diseases that exhibits capacity for adherence, invasion, and colonization of host tissues, which allows alternating its location and damage in different sites of human body. This review aimed to discuss different studies that detected T. denticola in atherosclerotic plaques, demonstrating the importance of periodontal disease on the systemic health and the necessity of exploring the outcome of this colonization apart from the oral cavity. Methodology: Fifty-five studies were identified and gathered in this review according to the following topics: Periodontal disease, atherosclerosis and T. denticola. In vitro and in vivo studies published between 2002 and 2020 were searched on PubMed, raising relevant insights about the role of T. denticola and its association with the systemic disease, atherosclerosis, focusing on the bacterial tissue invasion and development of atherosclerosis. Results: After bibliographic review, it was possible to identify studies demonstrating the presence of T. denticola and other oral pathogens in cardiac or vascular tissues and in blood serum, as well, there is research in which other evidence of a relationship with atherosclerosis is shown. Conclusion: The invasion of periodontal pathogens and its toxins associated to the host’s immune and inflammatory response may contribute to the development of atherosclerosis.

scholarly journals TheTreponema denticolaChymotrypsin-Like Protease Dentilisin Induces Matrix Metalloproteinase-2-Dependent Fibronectin Fragmentation in Periodontal Ligament Cells

2010 ◽  
Vol 79 (2) ◽  
pp. 806-811 ◽  
Author(s):  
Di Miao ◽  
J. Christopher Fenno ◽  
John C. Timm ◽  
Nam Eok Joo ◽  
Yvonne L. Kapila
Keyword(s):  
Periodontal Disease ◽  
Periodontal Ligament ◽  
Alveolar Bone ◽  
Inflammatory Responses ◽  
Tissue Homeostasis ◽  
Matrix Metalloproteinase 2 ◽  
Periodontal Ligament Cells ◽  
Treponema Denticola ◽  
Periodontal Pathogens ◽  
Culture Supernatants

ABSTRACTPeriodontal disease is a bacterially mediated chronic inflammatory disease that results in destruction of the periodontal ligament (PDL) and alveolar bone that surround and support the dentition. While their precise roles are not well understood, periodontal pathogens, includingTreponema denticola, are believed to initiate the destructive inflammatory responses and dysregulation of tissue homeostasis that characterize the disease. These responses are believed to result from both proinflammatory effects of acylated bacterial membrane components (lipopolysaccharides and lipoproteins) and degradative effects of secreted bacterial proteases. Host-derived matrix metalloproteinases (MMPs) are key enzymes both in tissue homeostasis and tissue destruction. MMP expression is modulated in part by specific proteolytic fragments of fibronectin (FN), which are associated with periodontal disease. FN is a predominant extracellular matrix component in the periodontium. We examined the ability ofTreponema denticolaand its acylated outer membrane PrtP protease complex to induce both activation of MMP-2 and generation of FN fragments in human PDL cell culture supernatants.T. denticolaparent and isogenic mutant strains, as well as MMP-2 small interfering RNA and specific inhibitors of MMP-2 and PrtP activity, were used to examine protein expression, gelatinolytic activity, and FN fragmentation in culture supernatants.T. denticolaand its purified protease induced both MMP-2 activation and FN fragmentation. Here, we demonstrate that PrtP proteolytic activity induces the activation of MMP-2 and that active MMP-2 is required for FN fragmentation. These results suggest a specific mechanism by which theT. denticolaprotease may disrupt homeostatic processes required for the maintenance of periodontal health.

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