Anti-angiogenic effects of water extract of a formula consisting of Pulsatilla koreana, Panax ginseng and Glycyrrhiza uralensis

Human infection by enterovirus type 71 (EV71) can cause life-threatening meningo-encephalitis. Currently, there is no effective anti-EV71 therapy available. Since EV71 infection commonly involves skin lesions, we tested our hypothesis that water extract of Glycyrrhiza uralensis (G. uralensis) could inhibit the cytopathic effects of EV71 in a human foreskin cell line by using an XTT-based method. Our results showed that the water extract of G. uralensis at 3,000 μg/ml has only 30% cytotoxicity on host cells, and furthermore, that the water extract of G. uralensis at 0.1 μg/ml could effectively protect host cells against EV71 infection (p < 0.0001). The half maximal inhibitory concentration (IC50) was 0.056 μg/ml with a selective index greater than 50,000. The water extract of G. uralensis exerted its effects not only by preventing viral attachment (p < 0.0001), but also by inhibiting the penetration of the virus (p < 0.0001). EV71 infection caused cells to produce significant amounts of IFN-β (p = 0.0003). However, the anti-EV71 activity of the water extract of G. uralensis was not mediated by IFN. In conclusion, the water extract of G. uralensis possesses potent anti-EV71 effects with less cytotoxicity. Its low IC50 and high 50% cytotoxic concentration (CC50) values suggest that it is a promising anti-EV71 agent.

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Water extract of licorice (Glycyrrhiza uralensis Fisch.) Supplementation is related with decreased lipid peroxidation among healthy male smokers with glutathione-S-transferase M1 positive genotype

Food Science and Biotechnology ◽

10.1007/s10068-010-0071-5 ◽

2010 ◽

Vol 19 (2) ◽

pp. 511-516

Author(s):

Gyeong-Im Jeon ◽

Eunju Park ◽

Hye-Jin Lee ◽

Myung-Hee Kang

Keyword(s):

Lipid Peroxidation ◽

Water Extract ◽

Glycyrrhiza Uralensis ◽

Healthy Male ◽

Glutathione S Transferase ◽

Glycyrrhiza Uralensis Fisch

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Effect of Co-Administration of Panax ginseng and Brassica oleracea on Postmenopausal Osteoporosis in Ovariectomized Mice

Nutrients ◽

10.3390/nu12082415 ◽

2020 ◽

Vol 12 (8) ◽

pp. 2415

Author(s):

In Soon Kang ◽

Taiwo Samuel Agidigbi ◽

Young Min Kwon ◽

Dong-Gyu Kim ◽

Rang Ie Kim ◽

...

Keyword(s):

Bone Loss ◽

Brassica Oleracea ◽

Postmenopausal Osteoporosis ◽

Panax Ginseng ◽

Water Extract ◽

Hot Water ◽

Potential Candidate ◽

Mineral Density ◽

Ginseng Extract ◽

Ovariectomized Mice

Postmenopausal osteoporosis is a common disorder resulting from increased osteoclastic activity. To determine the effect of Panax ginseng on postmenopausal osteoporosis, ovariectomized (OVX) mice were treated with 500 mg/kg/day P. ginseng extract (Pg) alone or in combination with hot water extract of Brassica oleracea (Bo) daily for 10 weeks, and the effect of the treatments on OVX-induced bone loss was examined. Bone weight, bone mineral density (BMD), osteoclast (OC) formation, OC marker expression, and biochemical parameters in blood were determined. OVX significantly increased body weight and decreased bone weight compared with those in the Sham group (p < 0.01). Pg or Bo alone did not affect OVX-induced bone loss, but a combination of Pg and Bo (Pg:Bo) recovered bone weight. The bones of OVX mice showed lower BMD than that of Sham mice, and the Pg:Bo = 3:1 restored the decreased BMD. Single treatment with Pg or Bo did not alter OC formation; however, the Pg:Bo = 3:1 inhibited OC formation. In addition, Pg and Bo lowered the OVX-induced elevation in blood glucose level. Thus, we suggest that Pg in combination with proper materials, such as Bo, might be a potential candidate treatment with minimal side effects protect against postmenopausal osteoporosis.

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Evaluation of Anti-Oxidant Capacity of Root of Scutellaria baicalensis Georgi, in Comparison with Roots of Polygonum multiflorum Thunb and Panax ginseng CA Meyer

The American Journal of Chinese Medicine ◽

10.1142/s0192415x10008263 ◽

2010 ◽

Vol 38 (04) ◽

pp. 815-827 ◽

Cited By ~ 39

Author(s):

Enoch Chan ◽

Cynthia Ying-Kat Wong ◽

Chun-Wai Wan ◽

Ching-Yee Kwok ◽

Jian-Hong Wu ◽

...

Keyword(s):

Panax Ginseng ◽

Cell Damage ◽

Water Extract ◽

Scutellaria Baicalensis ◽

Total Phenolic ◽

Polygonum Multiflorum ◽

Traditional Practice ◽

Scutellaria Baicalensis Georgi ◽

Phenolic Contents ◽

Anti Oxidant

In Chinese communities, regular consumption of Chinese-medicated diets (CMD) (usually in the form of soup) is a traditional practice to promote health and prevent disease development. The overall improvement of health conditions is believed to be correlated with the anti-oxidant potentials of these herbs. Huangqin, roots of Scutellaria baicalensis Georgi (Lamiaceae), is one of the herbs commonly used in CMD. In this study, the anti-oxidant capacities of Huangqin extracts (water, ethanol and ether extracts) were evaluated and compared to commonly used CMD herbs, Heshouwu, roots of Polygonum multiflorum Thunb (Polygonaceae) and Renshen (or Ginseng), roots of Panax ginseng CA Meyer (Araliaceae). The anti-oxidant capacities were measured by using both cell-free assay [ferric reducing/anti-oxidant power (FRAP)] and biological methods [2,2'-azobis–(2-amidinopropane) (AAPH)-induced haemolysis assay and H2O2 -induced cell damage on H9C2 cells]. Additionally, the total phenolic content was measured using Folin-Ciocalteu methods. Water extract of Huangqin has the highest anti-oxidant activities compared to the ethanol and ether extracts. A positive relationship between the anti-oxidant effects and total phenolic contents of extracts was demonstrated. This shows that Huangqin could be an effective dietary anti-oxidant that can be consumed regularly as a functional food for the prevention of oxidant/free radical-related diseases.

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