Edible Culture Media from Cereals and Soybeans for Pre-cultivation of Lactic Acid Bacteria

The capacity of three phosphates to interrupt the lytic cycle of four specific autochthonal bacteriophages of lactic acid bacteria used as starters was assayed. The phosphates used (polyphosphates A and B and sodium tripolyphosphate–high solubility [TAS]) were selected on the basis of their capacity to sequester divalent cations, which are involved in the lytic cycle of certain bacteriophages. The assays were performed in culture media (deMan Rogosa Sharpe and Elliker broths) and reconstituted (10%, wt/vol) commercial skim milk to which phosphates had been added at concentrations of 0.1, 0.3, and 0.5% (wt/vol). Phosphate TAS was the most inhibitory one, since it was able to inhibit the lytic cycle of all bacteriophages studied, in both broths and milk. In broth, polyphosphates A and B inhibited the lytic cycle of only two bacteriophages at the maximal concentration used (0.5%), whereas in milk, they were not capable of maintaining the same inhibitory effect.

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Screening and enumeration of lactic acid bacteria in milk using three different culture media in Petrifilm™ Aerobic Count plates and conventional pour plate methodology

Journal of Dairy Research ◽

10.1017/s002202990700266x ◽

2007 ◽

Vol 74 (4) ◽

pp. 387-391 ◽

Cited By ~ 14

Author(s):

Maria BT Ortolani ◽

Gabriela N Viçosa ◽

Vanerli Beloti ◽

Luís A Nero

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Culture Media ◽

Skim Milk ◽

Raw Milk ◽

Excellent Correlation ◽

Anaerobic Conditions ◽

Milk Samples ◽

Experimental Inoculation

This study aimed to compare Petrifilm™ Aerobic Count (AC) plates and the conventional pour plate methodology using de Mann-Rogosa-Sharpe (MRS), Kang-Fung (KF) and Kang-Fung-Sol (KFS) culture media for screening and enumeration of lactic acid bacteria (LAB) in milk. Suspensions of 10 LAB species in reconstituted powder skim milk and 30 raw milk samples, without experimental inoculation, were tested. For selective enumeration, all samples were previously diluted in MRS, KF and KFS broths and then plated in Petrifilm™ AC and conventional pour plate methodology, using the same culture media with added agar. All plates were incubated at 37°C for 48 h in anaerobic conditions. Differences in the counts were observed only for raw milk samples using KFS in conventional methodology, when compared with the counts obtained from MRS and KF (P⩽0·05). The results showed excellent correlation indexes between both methodologies using the three culture media for LAB suspensions (r=0·97 for MRS, KF and KFS). For raw milk samples, the correlation indexes were excellent (r=0·97, for MRS) and good (r=0·84 for KF, and r=0·82 for KFS), showing some interference in Petrifilm™ AC when supplements were added, especially lactic acid. These results indicate the possibility of using Petrifilm™ AC plates for enumeration of LAB in milk, even with the use of selective supplements.

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Antimicrobial Action of Hydrolyzed Chitosan against Spoilage Yeasts and Lactic Acid Bacteria of Fermented Vegetables

Journal of Food Protection ◽

10.4315/0362-028x-65.5.828 ◽

2002 ◽

Vol 65 (5) ◽

pp. 828-833 ◽

Cited By ~ 62

Author(s):

TONY SAVARD ◽

CAROLE BEAULIEU ◽

ISABELLE BOUCHER ◽

CLAUDE P. CHAMPAGNE

Keyword(s):

Molecular Weight ◽

Cell Wall ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Culture Media ◽

Antimicrobial Properties ◽

Spoilage Yeasts ◽

Entire Cell ◽

Fermented Vegetables ◽

Chitosan Lactate

The antimicrobial properties of various chitosan-lactate polymers (ranging from 0.5 to 1.2 MDa in molecular weight) against two yeasts isolated from fermented vegetables and against three lactic acid bacteria from a mixed starter for sauerkraut on methylene blue agar (MBA) and in vegetable juice medium (VJM) were investigated. Chitosan-lactate reduced the growth of all microorganisms in solid (MBA) as well as in liquid (VJM) medium. In MBA, a concentration of 5 g/liter was needed to inhibit the growth of Saccharomyces bayanus, while 1 g/liter was sufficient to inhibit the growth of Saccharomyces unisporus. Lactic acid bacteria were also inhibited in this range of concentrations. The low-molecular-weightchitosan-lactateDP3 (0.5 kDa) was most efficient in solid medium (MBA), and inhibitory activities decreased with increasing hydrolysate lengths. In liquid medium (VJM), 0.5 g of chitosan-lactate per liter reduced the growth rates for both yeasts, but 10 g/liter was insufficient to prevent yeast growth. Intermediate-molecular-weight chitosan-lactate (5 kDa) was more efficient than chitosan of low molecular weight. Native chitosan (1.2 MDa) showed no inhibition in either medium. Microscopic examination of S. unisporus Y-42 after treatment with chitosan-lactate DP25 showed agglutination of a refractive substance on the entire cell wall, suggesting an interaction between chitosan and the cell wall. When chitosanase was added to the culture media containing chitosan-lactate, refractive substances could not be observed.

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Stimulation of Bacteriocin Production by Dialyzed Culture Media from Different Lactic Acid Bacteria

Current Microbiology ◽

10.1007/s00284-004-4415-3 ◽

2005 ◽

Vol 50 (4) ◽

pp. 208-211 ◽

Cited By ~ 4

Author(s):

J.A. Vázquez ◽

M.P. González ◽

M.A. Murado

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Culture Media ◽

Bacteriocin Production ◽

Stimulation Of

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Probiotics and Postbiotics as Substitutes of Antibiotics in Farm Animals: A Review

Animals ◽

10.3390/ani11123431 ◽

2021 ◽

Vol 11 (12) ◽

pp. 3431

Author(s):

Daria Zamojska ◽

Adriana Nowak ◽

Ireneusz Nowak ◽

Ewa Macierzyńska-Piotrowska

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Large Scale ◽

Culture Media ◽

Body Weight Gain ◽

Antimicrobial Effect ◽

Antagonistic Activity ◽

Antagonistic Effect ◽

Farm Animals ◽

The European Union

Since 2006, the use of growth-promoting antibiotics has been banned throughout the European Union. To meet the expectations of livestock farmers, various studies have been carried out with the use of lactic acid bacteria. Scientists are trying to obtain the antimicrobial effect against the most common pathogens in large-scale farms. Supplementing the diet of broilers with probiotics (live, nonpathogenic microorganisms) stabilized the intestinal microbiota, which improved the results of body weight gain (BWG) and feed intake (FI). The positive effect of probiotics based on lactic acid bacteria has been shown to prevent the occurrence of diarrhea during piglet weaning. The antagonistic activity of postbiotics (inanimate bacteria, cell components, or post-fermentation by-products) from post-culture media after lactobacilli cultures has been proven on Staphylococcus aureus—the pathogen most often responsible for causing mastitis among dairy cows. The article aims to present the latest research examining the antagonistic effect of lactic acid bacteria on the most common pathogens in broilers, piglets, pigs, and cow farms.

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Optimization of an effective growth medium for culturing probiotic bacteria for applications in strict vegetarian food products

Functional Foods in Health and Disease ◽

10.31989/ffhd.v2i10.75 ◽

2012 ◽

Vol 2 (10) ◽

pp. 369 ◽

Cited By ~ 5

Author(s):

Manju Pathak ◽

Danik Martirosyan

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Growth Medium ◽

Culture Medium ◽

Culture Media ◽

Plant Seed ◽

Meat Extract ◽

Colony Forming Units ◽

Lactobacillus Lactis ◽

De Man

Background: This study aimed to modify de Man Rogosa Sharpe culture medium (termed MRS) for selective cultivation of probiotics strain for the consumption by the strictly vegetarian human population. Vegetarian probiotic foods by definition must be free from all animal-derived ingredients. This not only includes the product ingredients but the probiotic inoculum as well. Probiotic starter cultures are traditionally grown and stored in media containing milk or meat-derived ingredients. The presence of these ingredients makes the probiotic cell concentrates unsuitable for use in vegetarian products and thus creates the need for a growth medium which is free from animal-derived ingredients. Present study investigated the growth of a strain of Lactobacillus lactis in MRS. The present invention relates in general to a bacterial culture media, and more specifically a complex microbial culture media, based on plant seed powder extract in place of animal extract for probiotic bacterial growth.Methods: Lactobacillus lactis, a probiotic, was grown in standard MRS culture medium as well as in our various test media (TM) containing various vegetal source in place of beef extract, yeast extract and peptone as in case of MRS. The inoculated culture mediums were incubated at 37C for 72 hours and growth of probiotic is recorded at regular intervals. The growth was recorded as Colony Forming Units (CFUs).Results: The best growth of probiotic is observed in TM 2. TM 2 is the leguminous seed extract. Starter culture mediums for probiotics or other bacteria primarily contain protein from animal source. The possibility of using vegetal protein from TM 2 extract in place of peptones and meat extract for the nitrogen supplementation of culture media for the growth of lactic acid bacteria has been demonstrated. Conclusion: The absolute vegetarian culture medium containing TM 2 is better than standard MRS for the growth of probiotics.Abbreviations: de Man Rogosa Sharpe (MRS), Colony Forming Units (CFU), test media (TM), National Dairy Research Institute (NDRI), Tamarind seed powder (TSP), solid-state fermentation (SSF), Lactobacillus casei Shirota (LcS)Keywords: probiotics, lactic acid bacteria, vegetarian

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