scholarly journals DEVELOPMENT OF PIGEON PEA INOCULATED WITH RHIZOBIUM ISOLATED FROM COWPEA TRAP HOST PLANTS

2016 ◽  
Vol 29 (4) ◽  
pp. 789-795 ◽  
Author(s):  
SALOMÃO LIMA GUIMARÃES ◽  
LAURA CRISTINA REZENDE DAS NEVES ◽  
EDNA MARIA BONFIM-SILVA ◽  
DANIELA TIAGO DA SILVA CAMPOS

ABSTRACT Pigeon pea is an important protein source grown in several tropical and sub-tropical countries, and is considered a multi-purpose plant that is resistant to the conditions of the Brazilian Cerrado. Among the possible uses for cowpea, its use as a green manure, increasing soil nitrogen content through the association with diazotrophic bacteria, generically known as rhizobia, is noteworthy. The present work aimed to evaluate the efficiency of Rhizobium strains isolated from cowpea plants in the development of pigeon peas cultured in Red Latosol. The experiment was conducted in a greenhouse, using a completely randomized design with seven treatments and four replications. Treatments consisted of inoculation with four Rhizobium strains (MT8, MT15, MT16, and MT23) and one commercial inoculant comprising Bradyrhizobium spp. strains BR 2801 and BR 2003. There were two controls, one absolute (without inoculation or nitrogen fertilization) and the other with nitrogen fertilization. Each experimental plot consisted of an 8-dm3 vase containing three plants. Analyzed variables included plant height, SPAD index, number and dry weight of nodules, and shoot and root dry masses. Pigeon peas responded significantly to inoculation treatment, since all the plants inoculated with Rhizobium strains isolated from cowpea strains showed results similar to plants in the nitrogen control and commercial inoculant treatments. This demonstrates a favorable plant-bacteria interaction, which can be utilized as an alternative nitrogen source for pigeon peas.

2018 ◽  
Vol 9 (3) ◽  
pp. 211-216
Author(s):  
Steffani Silferansti Tak ◽  
Irdika Mansur ◽  
Prijanto Pamungkas

Gosale (Syzygium malaccense (L.) Merr. & L.M.Perry) that belongs to the Myrtaceae family is one of the leading local plant species grown in Halmahera, North Maluku. Some problems in gosale breeding such as limited seed availability, low seed production due to seasonal fruiting and the nature of seeds that can not be stored for a long period (recalcitrant), suggest an alternative new production technique through a vegetative propagation (shoot cutting). Moreover, Improved seedling quality can be attempted by AMF inoculation, in which AMF at shoot cuttings play a role in initiating the formation of adventive roots. This study was conducted for 9 months using completely randomized design (CRD) with indigenous AMF inoculation treatment. The results showed that gosale cuttings that planted with indigenous AMF inoculation were effective at all observed variables. Inoculum AMF inoculated without the addition of host plant Pueria javanica at gosale shoot cuttings suggested the best treatment. The treatment of inoculum AMF from Kusumadehe (M1) obtained the best result in the variable of plant height increase, dry weight of shoot, dry weight of root, root shoot ratio), and seedling quality index. While the treatment of Inoculum AMF from Soakonora revealed the highest result in the variable of plant diameter increase and AMF colonization.Keywords: gosale, shoot cuttings, AMF, Pueraria javanica, West Halmahera


AGRICA ◽  
2020 ◽  
Vol 3 (2) ◽  
pp. 138-150
Author(s):  
Kristina Erniyani

Bacteria Bradyrhizobium spp. From nodules on soybean plants, bind nitrogen from the air and make it freely available to plants. Amount of nitrogen fixed depends on the ability of a strain to form nodules, nodule effectiveness and environmental conditions. On land – land that had been planted with soybeans, inoculation with Bradyrizhobium bacteria are often not required. To test these lands in the district of Ende, NTT has conducted an experiment with a factor in the design of Randomized Complete with nine treatment groups. The treatment consists of (i) inoculation with the soybean crop in the ground former Ekoae (R1), (ii) inokulasi with soil former soybean plants in Ndona (R2), (iii) inoculation of soybean plants with the soil used in Flores (R3), (iv) Brady rhizobium isolates from soybean nodules in Ekoae (R4), (v) Bradyrhizobium isolates from nodules of soybean plants is in Ndona (R5), (v) Bradyrhozobium isolates from nodules of soybean plants at Flores (R6) (vii) a commercial inoculant legin (L), (viii) fertilizer nitrogen (N), and (ix) Control (K). all treatments were repeated 4 times. Land used in these experiments is the land of Ndona. The experiment was carried out since faculty, Udayana University. The highest percentage of effective nodules on the treatment Bradyrhizobium isolates from sybean nodules in Ndona (R5). Total nitrogen levels at the age of 45 HST in the highest in soybean plants that received 100 kg ha-1 urea and soybeans that have a desolate Bradyrhizobium inoculation of soybean nodules in Ndona (R). Efficiency and levels of symbiotic nitrogen fixation result obtained highest in soybean plants that received an inoculation Bradyrhizobium diisolat soybean nodules in Ndona (R5). Oven dry weight of plants that from the age of 45 HST on all isolates are equivalent to the oven-dry weight of plants at the fertilization of 100 kg ha- 1 urea. The result shoed that the land – the land of Ekoae, Ndona and Fkres have high Bradirhizobium contain bacteria. Bacteria Bradyrhizobium cp. Most numerous in the isolates from soybean nodules in Ndona (R5) the most efficient from effective nodules is 82.26% and as high as the 0.52% N fixate. These results indicate that isolates from Ndona Bradyrhizobiun veeb able to form effective nonudel on yoy beans grown in soil from Ndona. The result also indicates inoculation with a commercial inoculant was obtained not on land – land planted with soybeans used


2017 ◽  
Vol 3 (2) ◽  
pp. 101
Author(s):  
Tri Retno Dyah ◽  
Srikandi Srikandi

Quality Improvement  of  Liquid  Organic  Fertilizer from Water Waste  of Biogas Using Compostar          It had been improved the quality of the liquid organic fertilizer from waste of Biogas production with the addition of inoculant ( F1 and F2 ) and without inoculant (F0) also additives ( V1 , V2 and V3 ) . Variations of a given treatment comprised of F0V0 (as control), F0V1 , F0V2 , F0V3 , F1V1 , F1V2 , F1V3, F2V1 , F2V2 and F2V3 using experimental design of CRD (Completely Randomized Design) with 3 x replications . Liquid fertilizer quality parameters measured were : temperature , pH , organic-C , total-N and P. While the parameters measured in the corn biomass is wet weight , dry weight , levels of N and P. The results showed that the addition of biofertilizer Compostar isolates (F2) were able to decompose the form of additives to the maximum compared with (F1) and without (F0) the addition of commercial inoculant  with an average content of N - total of  823.2 mg / L and the average P2O5  content of 187.7 mg / L. While the addition of additives could increase levels of  N - total and P2O5 , where the levels of  N - largest total in the treatment of V3 ( Tp.fish Tp.bone 2 % and 2% ) with an average of 967.6 mg / L , while the largest concentration of P2O5 in treatment V1 ( Tp.fish 2 % ) with an average of 154.4 mg / L. Liquid organic fertilizer did not significantly affect the levels of nutrient uptake , biomass dry weight of corn plants , but had significant effect on plant height , stem large and heavy wet corn crop biomass , was the treatment of F2V2 with the addition of biofertilizer that isolates composter and additives in the form of bone meal 2 % .Keywords :  liquid organic fertilizer, biogas sludge, compostar ABSTRAK        Telah dilakukan peningkatan kualitas pupuk organik cair dari limbah produksi biogas dengan penambahan inokulan   ( F1 dan F2)  dan tanpa penambahan inokulan (F0) serta penambahan zat aditif (V1, V2 dan V3).   Variasi perlakuan yang diberikan  terdiri dari  F0V0  sebagai kontrol, F0V1 , F0V2 , F0V3 ,  F1V1 , F1V2 , F1V3  , F2V1 , F2V2 , dan F2V3   menggunakan rancangan percobaan RAL ( Rancang Acak Lengkap) dengan 3 x ulangan. Parameter kualitas pupuk cair yang diukur , yaitu : suhu, pH, C-organik, N-total dan P. Sedangkan parameter pada biomassa  jagung yang diukur adalah bobot basah, bobot kering, Kadar hara N dan P. Hasil yang diperoleh menunjukkan bahwa penambahan biofertilizer berupa isolate Compostar (F2) mampu menguraikan zat aditif secara maksimal dibandingkan dengan  inokulan komersil (F1) dan tanpa penambahan inokulan (F0) dengan rataan kandungan N-Total sebesar  823,2 mg/L  dan rataan kandungan P2O5 sebesar 187,7 mg/L. Sedangkan penambahan zat aditif mampu meningkatkan kadar N-Total dan P2O5, dimana kadar N-Total terbesar pada perlakuan V3( Tp.ikan 2% dan Tp.Tulang 2%) dengan rataan sebesar 967,6 mg/L, sedangkan kadar P2O5 terbesar pada perlakuan V1 (Tp.ikan 2%) dengan rataan sebesar 154,4 mg/L. Pemberian pupuk organic cair tidak berpengaruh nyata terhadap serapan kadar hara, berat kering biomassa tanaman jagung, tetapi berpengaruh nyata terhadap tinggi tanaman,besar batang dan berat basah biomassa tanaman jagung, yakni pada perlakuan F2V2 yaitu  dengan penambahan biofertilizer isolate composter dan zat aditif berupa tepung tulang 2%.Kata Kunci : pupuk organik cair, sludge biogas, compostar.


2020 ◽  
Vol 38 (2) ◽  
pp. 185-191
Author(s):  
Caliane da S Braulio ◽  
Rafaela SA Nóbrega ◽  
Luís Cláudio V Silva ◽  
Andreza de J Correia ◽  
Cheila BC de Sousa ◽  
...  

ABSTRACT Inoculating crops with diazotrophic bacteria constitutes a feasible biotechnological tool to reduce or even avoid the use of nitrogen fertilizers and to improve the nutritional status of crops. However, the potential use of such technology is limited due to the lack of studies in diverse vegetable crops. We evaluated the production of chicory (Cichorium intybus) in response to inoculation with diazotrophic bacteria in two soil classes. The experiments were performed under greenhouse conditions and under a completely randomized design. The treatments were constituted by strains UFRB FA34C2-2, UFLA 03-84 and INPA 0311B, and two control treatments (devoid of bacterial inoculation and with and without nitrogen fertilization). Two experiments were carried out, one using a soil classified as a dystrophic yellow Latosol (Oxisoil) and one using a Quartsandy Neosol (Typic Quartzipsamment). Chlorophylls a, b and total; plant height; number of leaves; dry mass of the aerial portion; dry mass of roots; total dry mass; effectiveness; efficiency in addition to N and P gathering in the aerial portion, were evaluated 60 days after seeding. The inoculation with diazotrophic bacteria resulted in adequate production when compared to the control treatment without nitrogen fertilization and without inoculation. Diazotrophic bacteria showed potential supplying nitrogen to chicory plants and spare the use of nitrogen fertilization in both soil classes. The strain INPA 0311B, recommended for cowpea, promoted vegetal growth and increased the dry weight of the aerial portion in chicory, under both dystrophic yellow Latosol (Oxisoil) and Neossolo Quartzarênico (Typic Quartzipsamment), when cultivated under greenhouse conditions.


2006 ◽  
Vol 41 (3) ◽  
pp. 439-447 ◽  
Author(s):  
Ruy Raposeiras ◽  
Ivanildo Evódio Marriel ◽  
Maria Rita Scotti Muzzi ◽  
Edilson Paiva ◽  
Israel Alexandre Pereira Filho ◽  
...  

The objective of this work was to identify the most competitive and effective Rhizobium strains in order to increase common bean yield by nitrogen fixation as alternative or complementation to the nitrogen fertilization. Competitiveness tests were lead in axenic conditions, in Cerrado soil pots and in three field experiments, with native Rhizobium strains that were previously identified, according to their effectiveness and genetic variability. The identification of strains in nodules was performed using serological tests (axenic conditions) - agglutination and enzyme linked immunosorbent (Elisa) assays - and random amplified polymorfic DNA (RAPD) (Cerrado soil). Plant yield was determined using the dry weight (greenhouse conditions), total N and grain yield (field experiments). Among the analyzed Rhizobium strains, native strain SLA 2.2 and commercial strain CIAT 899 were the dominant nodules in plants of the most productive plots, presenting yield productivity similar or higher to those obtained in treatments where 20 kg ha-1 of N were applied.


2014 ◽  
Vol 1 (1) ◽  
pp. 25-29
Author(s):  
Rahim Mohammadian ◽  
Behnam Tahmasebpour ◽  
Peyvand Samimifar

A factorial experiment was conducted with a completely randomized design to evaluate the effects of planting date and density on calendula herbs and peppermint. It had 3 replicates and was done in Khosroshahr research farm, Tabriz in 2006. Under studied factors were: 3 planting dates (10 May, 25 May and 10 June) in 4 densities (25, 35, 45, 55) of the plant in square meters. The results of variance a nalysis showed that there was 1% probability significant difference between the effects of planting date and bush density on the leave number, bush height and the bush dry weight. But the mutual effect of the plant date in mentioned traits density was insignificant. Regarding the traits mean comparison, the total maximum dry weight was about the 55 bush density in mm. Also, the bush high density in mm causes the bush growth and its mass reduction. When there is the density grain, the flower number will increase due to bush grain in surface unit. Overall, we can conclude that 10 June planting and 45 bush density in mm is the most suitable items and results in favored production with high essence for these crops.


2018 ◽  
Vol 7 (1) ◽  
pp. 28
Author(s):  
Yetti Elidar

Research on the response of roots of palm sugar palm seeds (Arenga pinnata) in nurseries at doses and intervals of Nasa liquid organic fertilizer. Aims to determine the dosage, interval and combination of dosages and fertilization intervals with Nasa liquid organic fertilizer which can provide the best dry weight of the roots in the nursery. The research design used was a Completely Randomized Design (CRD) with 3x3 factorial experiments and each treatment was repeated 8 (eight) times, consisting of: the first factor was the treatment of POC Nasa dose in a concentration of 3 cc POC Nasa per liter of water (D) consists of 3 levels, namely: d1 = 300 ml POC Nasa, d2 = 400 ml POC Nasa, d3 = 500 ml POC Nasa, while the second factor is the treatment of POC Nasa Interval (I) consisting of 3 levels, namely: i1 = 2 once a week, i2 = once every 3 weeks, i3 = once every 4 weeks. The results of the POC Nasa dose study had a significant effect on leaf wet weight, leaf dry weight, root wet weight and root dry weight. The best dose at this level are: d2 (400 ml of Nasa liquid organic fertilizer), the interval of liquid organic fertilizer Nasa has a significant effect on leaf wet weight, leaf dry weight, root wet weight and root dry weight. The best dose at this level is: i1 (once every 2 weeks). The treatment combination has no significant effect on all parameters. 


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Amanullah ◽  
Shah Khalid ◽  
Farhan Khalil ◽  
Mohamed Soliman Elshikh ◽  
Mona S. Alwahibi ◽  
...  

AbstractThe dry matter partitioning is the product of the flow of assimilates from the source organs (leaves and stems) along the transport route to the storage organs (grains). A 2-year field experiment was conducted at the agronomy research farm of the University of Agriculture Peshawar, Pakistan during 2015–2016 (Y1) to 2016–2017 (Y2) having semiarid climate. Four summer crops, pearl millet (Pennisetum typhoidum L.), sorghum (Sorghum bicolor L.) and mungbean (Vigna radiata L.) and pigeonpea (Cajanus cajan L.) and four winter crops, wheat (Triticum aestivum L.), barley (Hordeum vulgare L.), fababean (Vicia faba) and rapeseed (Brassica napus) were grown under two irrigation regimes (full vs. limited irrigation) with the pattern of growing each crop either alone as sole crop or in combination of two crops in each intercropping system under both winter and summer seasons. The result showed that under full irrigated condition (no water stress), all crops had higher crop growth rate (CGR), leaf dry weight (LDW), stem dry weight (SDW), and spike/head dry weight (S/H/PDW) at both anthesis and physiological maturity (PM) than limited irrigated condition (water stress). In winter crops, both wheat and barley grown as sole crop or intercropped with fababean produced maximum CGR, LDW, SDW, S/H/PDW than other intercrops. Among summer crops, sorghum intercropped either with pigeon pea or with mungbean produced maximum CGR, LDW, SDW, and S/H/PDW at both growth stages. Sole mungbean and pigeon pea or pigeon pea and mungbean intercropping had higher CGR, LDW, SDW, S/H/PDW than millet and sorghum intercropping. On the other hand, wheat and barley grown as sole crops or intercropped with fababean produced maximum CGR, LDW, SDW, and S/H/PDW than other intercrops. Fababean grown as sole crop or intercropped with wheat produced higher CGR, LDW, SDW, and S/H/PDW at PM than intercropped with barley or rapeseed. From the results it was concluded that cereal plus legume intercropping particularly wheat/fababean in winter and sorghum/pigeon pea or sorgum/mungbean in summer are the most productive intercropping systems under both low and high moisture regimes.


2021 ◽  
Vol 4 (2) ◽  
pp. 291
Author(s):  
Indri Elang Mayanti ◽  
Basir Achmad

The areas of tropical forests in Indonesia always decrease every year, so that efforts are needed to cultivate plants efficiently for the supply of seedlings. Sungkai (Peronema canescens) has good quality wood that can be used for various purposes, either for construction, furniture, plywood or the leaves can be used for medicine. The purpose of this study was to analyze the growth of stem cuttings of sungkai based on the number of latent buds by calculating the root dry weight, bud dry weight, and total dry weight. This study used a factorial complete randomized design. There were two factors studied, namely the number of buds consisting of 2 levels: 2 buds and 1 bud, and the stem split factor. Each treatment was replicated 3 times and each experiment unit used 5 cuttings. The growth of sungkai cuttings in the treatment of two buds had a total dry weight of 170.04 grams, while the treatment of one bud had a total dry weight of 98.30 grams. The results of this study indicated that the more the number of latent buds, the more root and bud growth of cuttings.Keywords: Coppice; Latent buds; Cuttings; Sungkai


Author(s):  
Alinne da Silva ◽  
◽  
Vinícius I. Franzini ◽  
Cristiano D. Piccolla ◽  
Takashi Muraoka ◽  
...  

ABSTRACT The common bean has been considered to have low biological nitrogen fixation capacity; however, this process can be made more effective with molybdenum (Mo) supplementation. The objective of this study was to evaluate the influence of Mo rates on the growth and biological nitrogen fixation by two Brazilian common bean cultivars using the 15N isotope dilution technique. The experiment was performed in 2014 in a completely randomized design arranged in a 5 x 3 factorial scheme, corresponding to 5 rates of Mo (control, 40, 80, 120 and 240 g ha-1), the common bean cultivars Aporé, Ouro Negro and NORH-54 (a non-nodulating common bean cultivar), and three replicates. The application of Mo and the inoculation with rhizobia strains contributed to improving nitrogen fixation and grain weight. The cultivar Ouro Negro showed a higher number and weight of nodules and a higher amount of nitrogen derived from the atmosphere than the cultivar Aporé. The biological nitrogen fixation of Aporé was more dependent on the application of Mo. These results indicated that inoculation with Rhizobium strains and Mo supply effectively contributed to biological nitrogen fixation and improving grain production.


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