scholarly journals Histomorphological Studies of the Organs of Malaria Mice Model After Administration Combination of Leaf and Stem Bark Extract of Syzygium cumini with Chloroquine

2019 ◽  
Vol 12 (1) ◽  
pp. 106-111
Author(s):  
Lilik Maslachah ◽  
Rahmi Sugihartuti ◽  
Retno Sri Wahyuni

Aim: This research aimed to determine the efficacy of Syzygium cumini L. as an adjuvant therapy on blood changes and splenic index of mice model malaria. Materials and Methods: Mice were infected intraperitoneally with 0.2 ml red blood cell (RBC) that contains 1×106 Plasmodium berghei. 35 mice were divided into seven treatment groups: Group K0: Mice were not infected; K1: Mice were infected; K2: Mice were infected and given chloroquine; P1: Mice were infected and given S. cumini leaf extract; P2: Mice were infected and given chloroquine and also S. cumini leaf extract; P3: Mice was infected and given S. cumini stem bark extract; and P4: Mice were infected and given chloroquine and S. cumini stem bark extract. Treatment was given for 4 days 24 h post-P. berghei infection. 21st day post-P. berghei infection, blood was taken from the heart for hematological examination, and the spleen was taken to examine the splenic index and also to measure the weight and length of the spleen. Hematological data and splenic index were analyzed by analysis of variance test, and if there is a difference, the test is continued by Duncan's multiple range test with 5% level. Results: The K0 group has normal hemoglobin (HGB), RBC, and hematocrit (HCT) and significantly different (p<0.05) than other groups. HGB, RBC, and HCT of K1 group were under normal range, lowest, and significantly different (p<0.05) than other groups. Mean corpuscular volume and mean corpuscular HGB values of K2 groups showed a decrease. The number of leukocytes, lymphocytes, and monocytes of K1 groups was increasing and significantly different (p<0.05) with K2 and treatment group. The length, width, weight, and splenic index of K1 group were significantly different (p<0.05) with K0 group. K2 and treatment groups showed that the length and width of spleens were significantly different (p<0.05) with K1. Conclusion: The combination of chloroquine with leaf and chloroquine with stem bark extract of S. cumini as adjuvant therapy may increase the amount of erythrocyte; decrease the number of leukocytes, lymphocytes, and monocytes; and decrease the length, width, and splenic index on malaria mice models.


2018 ◽  
Vol 24 ◽  
pp. 11-18
Author(s):  
M Hasanuzzaman ◽  
W Islam ◽  
MB Islam

Phytochemical screening of the secondary metabolites was performed with acetone, chloroform, methanol and n-hexane extracts of the leaves, roots, stem bark and seeds of the plant Syzygium cumini (Linn.) and were detected using various tests for identifying the isolated components. Acetone extract of the leaves showed the presence glycosides, phenols, proteins, resins and saponins while the stem bark extract showed the presence of alkaloids, flavonoids, glycosides, phenols, proteins, resins and saponins. All the above constituents except saponins were detected in the root extract. The seed extract contains alkaloids, carbohydrates, phenols, proteins and tannins. Chloroform extract of the leaves showed the presence of alkaloids, proteins and steroids while root extract contains alkaloids and steroids and that of seed extract alkaloids, carbohydrates, phenols, proteins and tannins, however, alkaloids and tannins were found in the stem bark extract. Methanol extract of the leaves and stem bark showed the presence of alkaloids, carbohydrates, flavonoids, glycosides, phenols, resins, saponins, steroids and tannins while the root extracts contain all the above constituents along with proteins. N-hexane extract of the leaves contain only alkaloids, roots contain alkaloids and resins and that of seeds contain carbohydrates and proteins while alkaloids, proteins and tannins were found in stem bark extract. The present findings reveal the presence of various medicinally important phytochemicals from the plant S. cumini extracts may have application in traditional system of medicine to cure various ailmentsJ. bio-sci. 24: 11-18, 2016


Author(s):  
James F. Amaku ◽  
Segun A. Ogundare ◽  
Kovo G. Akpomie ◽  
Comfort M. Ngwu ◽  
Jeanet Conradie

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Camila Gabriel Kato-Schwartz ◽  
Anacharis Babeto de Sá-Nakanishi ◽  
Ana Carolina Guidi ◽  
Geferson de Almeida Gonçalves ◽  
Fernanda Giacomini Bueno ◽  
...  

2020 ◽  
Vol 151 ◽  
pp. 01011
Author(s):  
Safrida Safrida ◽  
Mustafa Sabri

This study was designed to determine the effect of Carica papaya L. stem bark extracts on cholesterol concentration in rats induced with glibenclamide. A completely randomized design was used for the experiment which consisted of 6 treatment groups, each group consisted of four rats, as follows:1) KN (negative control, non-diabetic rats); KP, diabetic rats given glibenclamide 10 mg/kg body weight; EP 1, diabetic rats given 0 mg/kg body weight/day extract; EP2, diabetic rats given 100 mg/kg body weight/day extract; and EP3, diabetic rats given 200 mg/kg body weight/day extract, EP4, diabetic rats given 300 mg/kg body weight/day extract for 28 day. The results showed that C. papaya L. stem bark extract decreased (P<0.05) cholesterol levels in diabetic rats. It was concluded that C. papaya L. stem bark extract had potential as anti-hypercholesterolemic in diabetic rats.


2012 ◽  
Vol 5 (6) ◽  
pp. 314-321 ◽  
Author(s):  
A. Mohammed ◽  
S.B. Mada ◽  
H.M. Yakasai

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