scholarly journals Phytochemical screening of Syzygium cumini (L.) extracts in different solvents

2018 ◽  
Vol 24 ◽  
pp. 11-18
Author(s):  
M Hasanuzzaman ◽  
W Islam ◽  
MB Islam
Keyword(s):  
Stem Bark ◽  
Chloroform Extract ◽  
Seed Extract ◽  
Bark Extract ◽  
Root Extract ◽  
Syzygium Cumini ◽  
Phytochemical Screening ◽  
Traditional System ◽  
Hexane Extracts ◽  
Stem Bark Extract

Phytochemical screening of the secondary metabolites was performed with acetone, chloroform, methanol and n-hexane extracts of the leaves, roots, stem bark and seeds of the plant Syzygium cumini (Linn.) and were detected using various tests for identifying the isolated components. Acetone extract of the leaves showed the presence glycosides, phenols, proteins, resins and saponins while the stem bark extract showed the presence of alkaloids, flavonoids, glycosides, phenols, proteins, resins and saponins. All the above constituents except saponins were detected in the root extract. The seed extract contains alkaloids, carbohydrates, phenols, proteins and tannins. Chloroform extract of the leaves showed the presence of alkaloids, proteins and steroids while root extract contains alkaloids and steroids and that of seed extract alkaloids, carbohydrates, phenols, proteins and tannins, however, alkaloids and tannins were found in the stem bark extract. Methanol extract of the leaves and stem bark showed the presence of alkaloids, carbohydrates, flavonoids, glycosides, phenols, resins, saponins, steroids and tannins while the root extracts contain all the above constituents along with proteins. N-hexane extract of the leaves contain only alkaloids, roots contain alkaloids and resins and that of seeds contain carbohydrates and proteins while alkaloids, proteins and tannins were found in stem bark extract. The present findings reveal the presence of various medicinally important phytochemicals from the plant S. cumini extracts may have application in traditional system of medicine to cure various ailmentsJ. bio-sci. 24: 11-18, 2016

scholarly journals Antibacterial activities of sawdust and stem bark of Sasswood tree (Erythrophleum suaveolens, Guill. & Perr. Brenan, 1917) extracts against selected wood bacteria

2020 ◽  
Vol 36 (2) ◽  
pp. 52-61
Author(s):  
D.O. Ekhuemelo ◽  
C. Ekhuemelo ◽  
E.T. Tembe
Keyword(s):  
Ethyl Acetate ◽  
Antibacterial Properties ◽  
Stem Bark ◽  
Antibacterial Activities ◽  
Plant Diseases ◽  
Bark Extract ◽  
Phytochemical Screening ◽  
Zone Of Inhibition ◽  
Hexane Extracts ◽  
Stem Bark Extract

This study assessed the antibacterial properties of sawdust and stem bark of Erythrophleum suaveolens extracts on selected wood bacteria. Erythrophleum suaveolens samples were collected, dried and macerated by dissolving 1 Kg and 0.60 Kg of stem bark and sawdust respectively into 1 L of n-hexane, ethyl acetate and methanol. Sporfloxacin ciprofloxacin and cefuroxine antibiotics were used as control. The mixture was left for 24 hours then filtered and the filtrates evaporated to dryness. Qualitative phytochemical screening, zone of inhibition, minimum inhibitory and Bactericidal Concentrations (MIC/MBC) were determined according to standard methods. Tannins, steroids, saponins, glycosides, flavonoids, carbohydrates anthraquinones and alkaloids phytochemicals were present in E. suaveolens extracts. Zone of inhibition (32 – 37 mm) of antibiotics on test bacteria compared favourably with 17 – 24 mm of E. suaveolens extracts. Erythrophleum suaveolens ethyl acetate and methanol E. suaveolens extracts inhibited Staphylolococus aureus, Ralstonia solanacearum, Proteus mirabilis, Enterococcus faecium and Acidobacterium capsulatum growth at MIC of 10 mg/mL and n-hexane extracts at 20 mg/mL. At MBC of 20 mg/mL methanol stem bark extract completely killed most test bacteria. Methanol extracts were the most active extracts. The study has shown that E. suaveolens extracts can be explored in the control of plant diseases caused by test bacteria in the study. Key words: Antibacterial, E. suaveolens, extract, phytochemicals, zone of inhibition

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTI-DIABETIC ACTIVITY OF STEM BARK EXTRACT OF CALOTROPIS GIGANTEA LINN

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Harish Kumar ◽  
Sunil Sharma ◽  
Neeru Vasudeva
Keyword(s):  
Amino Acids ◽  
Medicinal Plants ◽  
Stem Bark ◽  
Bark Extract ◽  
Phytochemical Screening ◽  
Calotropis Gigantea ◽  
Traditional System ◽  
Stem Bark Extract ◽  
Phenolics Compounds

The present study aims to screen the preliminary photochemical present in the stem bark extracts of Calotropis gigantea. Calotropis gigantea Linn. is prevalently known as the aak, swallow-wort or milkweed. In the traditional system of medicine, the plant is used as one of the most important drugs to treat different diseases. The results showed the presence of phytochemical active compounds of alkaloids, flavanoids, glycosides, phenolics compounds, tannins, saponins, sterols, proteins, and amino acids in the stem bark extracts. Medicinal plants are the key source of bioactive compounds such as phenolics, tannins, alkaloids, and flavonoids which have been demonstrated in vitro to show anti-diabetic properties

scholarly journals IN-VIVO ANTI-DIARRHOEAL ACTIVITY AND PHYTOCHEMICAL SCREENING OF ETHANOL STEM BARK EXTRACT OF VITELLARIA PARADOXA GAERTN F. (SAPOTACEAE)

2020 ◽  
Vol 4 (3) ◽  
pp. 247-251
Author(s):  
Z. Abdullahi ◽  
A. A. Jimoh ◽  
B. E. Patrick ◽  
M. I. Yakubu ◽  
D. Mallam
Keyword(s):  
Castor Oil ◽  
Lethal Dose ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Experimental Models ◽  
Bark Extract ◽  
Phytochemical Screening ◽  
Vitellaria Paradoxa ◽  
Stem Bark Extract

Different parts of Vitellaria paradoxa plant have many applications in ethno-medicine. Some of the uses of this plant include treatment of diarrhoea and other GIT disorders. In this study the antidiarrhoeal activity of the ethanol extract of Vitellaria paradoxa was evaluated using three experimental models: Castor oil-induced diarrhoea; small intestinal motility and intestinal fluid accumulation (enteropooling) models in mice. Five groups of five mice were used for each model. Group one mice received 10 ml/kg of distilled water, while groups 2, 3, and 4 received 125, 250 and 500 mg/kg of the extract orally respectively. Group 5 mice received Loperamide 5 mg/kg orally. Oral median lethal dose (LD50) of the extract was determined using OECD (2008) Guideline 425. Phytochemical studies were conducted using standard procedures. The LD50 was estimated to be greater than 5000 mg/kg body weight and there were no signs of mortality or visible signs of toxicity in all the mice treated. Phytochemical screening revealed the presence of carbohydrates, alkaloids, flavonoids, saponins, tannins, triterpenes, steroids, cardiac glycosides and anthraquinones glycosides. Extract showed a dose-dependent anti-diarrhoeal activity by reducing stool frequency and consistency. The extract at the higher doses significantly (p < 0.05) inhibited GIT motility and castor oil-induced enteropooling, comparable to that of the reference control drug Loperamide. The study showed that ethanol stem bark extract of Vitellaria paradoxa possess anti-diarrhoeal activity and thus justifies its ethno-medicinal use in the treatment of diarrhoea.

scholarly journals Phytochemical screening and toxicological effects of Amblygonocarpus andongensis aqueous stem bark extract in wistar albino rat

2020 ◽  
Vol 14 (5) ◽  
pp. 107-112
Author(s):  
Aliyu Ebbo Abdullahi ◽  
Teleh Elsa Abdullahi ◽  
Udok Etuk Emmanuel ◽  
Jengebe Ladan Muhammad ◽  
Alhaji Saganuwan Saganuwan
Keyword(s):  
Stem Bark ◽  
Bark Extract ◽  
Phytochemical Screening ◽  
Albino Rat ◽  
Toxicological Effects ◽  
Stem Bark Extract

scholarly journals Qualitative phytochemical screening and antipyretic activity of methanolic stem bark extract of Mangifera indica in wistar rats

2020 ◽  
Vol 9 (4) ◽  
pp. 218-223
Author(s):  
Godfrey Mutuma Gitonga ◽  
◽  
Joseph Ngeranwa ◽  
Alex King’ori Machocho ◽  
Silas Kiruki ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Mangifera Indica ◽  
Stem Bark ◽  
Bark Extract ◽  
Phytochemical Screening ◽  
Antipyretic Activity ◽  
Stem Bark Extract

scholarly journals Comparative Phytochemical and in vitro Antioxidant Screening of the Root and Stem Bark of Annona muricata Linn

2020 ◽  
pp. 48-61
Author(s):  
C. E. Anarado ◽  
F. M. Chukwubueze ◽  
C. J. O. Anarado ◽  
N. L. Umedum ◽  
C. B. Nwanya
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Solvent Polarity ◽  
Stem Bark ◽  
Bark Extract ◽  
Root Extract ◽  
Annona Muricata ◽  
Stem Bark Extract

Aim: To compare the phytochemicals and antioxidant activities of stem bark and root extracts of Annona muricata. Methodology: The stem bark and root of Annona muricata were collected, washed, air-dried, ground and each extracted with methanol, ethyl acetate and n-hexane. The extracts were analysed for the presence of phytochemicals. Antioxidant screening was also carried out on the samples. Results: Cardiac glycosides were present in all the extracts of both root and stem bark. Alkaloids were present in moderate abundance in all the extracts except the ethyl acetate stem bark extract. Saponins and tannins were found in methanol extracts of both parts and also in very high abundance but the stem contained higher amount of saponins while alkaloids and tannins were found more in the root. Flavonoids were only found in the ethyl acetate stem bark extract.  Steroids were absent in all the extracts except n-hexane root extract. The root showed greater enzymatic antioxidant activities than the stem bark. The solvent polarity affected the phytochemical found in each extract.  The antioxidant activities of the catalase, superoxidase dismutase and glutathione peroxidase were significantly higher in the root of Annona muricata than in the stem. Conversely, peroxidase showed a significantly higher activity in the stem than in the root. Conclusion: The stem bark and root exhibited good antioxidant properties, so there is need to isolate the compounds responsible for antioxidant property exhibited by the plant parts.

scholarly journals Antimicrobial activities of extracts from Urtica urens

Food Research ◽  
2020 ◽  
Vol 4 (5) ◽  
pp. 1487-1492
Author(s):  
M.K. Pillai ◽  
R.P. Matamane ◽  
S.B. Mekbib
Keyword(s):  
Escherichia Coli ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Stem Bark ◽  
Antimicrobial Activities ◽  
Bark Extract ◽  
Root Extract ◽  
Fungal Isolates ◽  
Stem Bark Extract ◽  
Urtica Urens

The antibacterial and antifungal activities of various extracts from Urtica urens were evaluated against selected microorganisms using agar hole-plate diffusion method. A total of ten extracts namely U. urens hexane leaf extract (HLE), chloroform leaf extract (CLE), ethyl acetate leaf extract (ELE), methanolic leaf extract (MLE), hexane stem-bark extract (HSB), chloroform stem-bark extract (CSB), ethyl acetate stem-bark extract (ESB), methanolic stem-bark extract (MSB), chloroform root extract (CRT) and methanolic root extract (MRT) were prepared. These extracts were evaluated against two Gram-positive bacteria viz. Listeria monocytogenes and Staphylococcus aureus, four Gram-negative bacteria viz. Serratia marcescens, Pseudomonas aeruginosa, Escherichia coli (wild strain) and Escherichia coli (O157:H7) and two fungal isolates viz. Candida albicans and Penicillium digitatum. The inhibition zones of these extracts were found to be in the range of 9.0±0.0 to 40.5±0.7 mm against bacterial isolates and 16.0±4.2 to 17.5±4.9 mm against C. albicans. However, all these extracts exhibited no visible zone of inhibition against P. digitatum. Additionally, the minimum inhibitory concentrations (MICs) of these extracts were also determined and were found to be in the range of <31.25 to >1000 µg/mL against both bacterial and fungal isolates. From this study, we concluded that ELS, MLE, CRT and MRT showed promising antimicrobial activities.

scholarly journals In Vitro Anthelmintic Activity of Ethanol Stem Bark Extract of Albizia ferruginea (Guill. & Perr.) Benth

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Miriam Tagoe ◽  
Yaw Duah Boakye ◽  
Theresah Appiah Agana ◽  
Vivian Etsiapa Boamah ◽  
Christian Agyare
Keyword(s):  
Anthelmintic Activity ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Motility Assay ◽  
Bark Extract ◽  
Phytochemical Screening ◽  
Methanol Fraction ◽  
Test Concentration ◽  
Stem Bark Extract

Albizia ferruginea (Guill. & Perr.) Benth bark is used in the traditional medicine as a vermifuge. This study sought to determine the anthelmintic activity of the stem bark extract of Albizia ferruginea. The powdered A. ferruginea stem bark was extracted with ethanol. Phytochemical screening was carried out on A. ferruginea ethanol extract (AFE) and then screened for its anthelmintic property against Pheretima posthuma and Haemonchus contortus using the adult motility assay. The effect of AFE and its fractions on the anthelminthic activity of mebendazole and albendazole were also determined using the adult worm (P. posthuma) motility assay. AFE showed a dose-dependent anthelmintic activity against P. posthuma and H. contortus. The least concentration of AFE (0.5 mg/mL) paralyzed and killed P. posthuma within 272.50 ± 12.42  min and 354.50 ± 5.06  min of exposure, respectively. AFE at the least test concentration (0.14 mg/mL) caused paralysis and induced death of H. contortus, after at 63.50 ± 2.98 and 254.96 ± 2.44  min of exposure, respectively. AFE extract at 0.25 and 0.125 mg/mL increased the paralytic and helminthicidal activities of albendazole. The paralytic and helminthicidal activities of mebendazole were reduced when combined with AFE (0.25 and 0.125 mg/mL). Among the three fractions obtained from AFE, the methanol fraction showed the highest anthelmintic activity. The methanol fraction at 0.5 mg/mL caused paralysis after 69.90 ± 0.15  min and death of worm after 92.53 ± 0.74  min of exposure. The petroleum ether and ethyl acetate fractions showed relatively low anthelmintic activity. Phytochemical screening of AFE revealed the presence of tannins, saponins, glycosides, alkaloids, and coumarins. The results from this study show that A. ferruginea possesses anthelmintic activity which gives credence to its folkloric use.

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