Anti-Islet Cell Antibody and Anti-ovarian Antibody Levels in Iraqi Women with Polycystic Ovary Syndrome

Polycystic ovary syndrome (PCOS) is a mainly common endocrine disorder. Hyperandrogenism is the major standard in the diagnosisof PCOS development.Eighty women diagnosed with PCOS and twenty healthy women(aged 20-40 years)were enrolled in this study which was conducted at the infertility clinic of Kamal AL-Samaraay Hospital/ Baghdad. The diagnosis of PCOS was made according to the Rotterdam criteria. Body mass index (BMI, Kg/m2)was calculated by a specific formula. History of menstrual irregularity, hirsutism, acne, and alopecia was taken. Hormone levels in serum, namely luteinizing hormone (LH), follicle stimulating hormone (FSH), and testosterone were measured in all patients and control subjects.There was a statistically significant difference (P <0.05) in the BMI between the PCOS and control women.Also, the prevalence of overweight and obesity was much higher in PCOS women than in their matching control. The serum levels of LH, FSH, and testosterone were significantly different (P <0.05) in PCOS as compared to healthy women. Clinical hyperandrogenism (hirsutism and alopecia) were present in most of PCOS women, while acne wasa less useful clinical sign of hyperandrogenism.Inaddition, the prevalence of hirsutism and alopecia was increased in obese when compared with overweight and lean PCOS women.

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Two novel missense mutations in exon 9 of TPO gene in Polycystic Ovary Syndrome patients with hypothyroidism

Journal of Biotechnology Research Center ◽

10.24126/jobrc.2015.9.1.401 ◽

2015 ◽

Vol 9 (1) ◽

pp. 30-37

Author(s):

Mahdi Saber G. Al-Deresawi Al-Deresawi ◽

Abdul Hussein Moyet AlFaisal AlFaisal

Keyword(s):

Polycystic Ovary Syndrome ◽

Polycystic Ovary ◽

Missense Mutations ◽

Ovary Syndrome ◽

Reading Frame ◽

Iraqi Women ◽

Exon 9 ◽

The Relationship ◽

Hormonal Assay ◽

Fertile Women

This study was reflected on the relationship between the polycystic ovary syndrome (PCOS) and the geneticalternations in TPO gene. Fifty infertile Iraqi women with PCOS and 20 healthy women were included in thisstudy, Blood samples were collected from the Infertility center of AL-Yarmok Teaching Hospital in Baghdad,during the period from November, 2010 to May, 2011. The age of infertile and fertile women was ranged from 16 to45 years. The results of hormonal assay were as follows: There is significant (P ≤0.05) decrease in E2 and FSHlevels in PCOS women and fertile women, There is significant (P ≤0.05) increase in LH levels in PCOS women andfertile women. There is no significant differences in Testosterone levels and the ratio of LH/FSH was ≥1.5. Themolecular study was focused on the 18% of PCOS women with hypothyroidism. By sequencing for 27 samples; twonovel different mutations were identified in the reading frame of the TPO gene in transcript variant of exon 9:c.1471delC (deletion C in codon 460) and c.1481delC (deletion C in codon 464). The percentage of mutationsc.1481delC and c.1471delC recorded 55% and 44% of PCOS with hypothyroidism; respectively.

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Serum prolactin, Preptin, CCL 18 and genetic polymorphisms in Iraqi women with polycystic ovary syndrome.

Baghdad Science Journal ◽

10.21123/bsj.2021.18.4(suppl.).1552 ◽

2021 ◽

Vol 18 (4(Suppl.)) ◽

pp. 1552

Author(s):

Israa F. Ascar ◽

Areej Sh. Hameed

Keyword(s):

Polycystic Ovary Syndrome ◽

Polycystic Ovary ◽

Serum Prolactin ◽

Control Group ◽

Serum Samples ◽

Ovary Syndrome ◽

Private Laboratory ◽

Iraqi Women ◽

Automated Immunoassay ◽

Gene Expression Levels

The polycystic ovary syndrome is an endocrine condition. One of the leading causes of female infertility and the most common disorder among women. The work was being carried out on 100 Iraqi women (50 cases confirmed with PCOS and 50 controls). Between October 2019 and March 2020, blood samples were collected from the Advanced Institute of Infertility Diagnosis and Assisted Reproductive Technology at AL-Nahrain University and a private laboratory. ELISA was used to evaluate the biochemical parameters of preptin, FSH, insulin, LH, and CCL 18 in serum samples from the AFIAS-6 (AFIAS Automated Immunoassay System). The findings of the analysis indicate that, as opposed to the control group, values of prolactin (ng/ml), LH (mIU/ml), Preptin (pg/ml) and CCL 18 (ng/ml) Quite higher in PCOS sickness (p < 0.001) Compared with the patient group, the values of testosterone (ng/ml) and FSH (mIU/ml) was noticeably higher (p <0.05), and PRLR gene expression levels in PCOS patients were significantly increased by 3.6 times. I n summary, the levels of Preptin and CCL18 can be regarded as PCOS markers.

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Relationship of follicle stimulating hormone receptor Gene Thr307Ala polymorphism with polycystic ovary syndrome in Iraqi women

American Journal of BioMedicine ◽

10.18081/2333-5106/019-4/342-353 ◽

2019 ◽

Vol 7 (4) ◽

pp. 342-353

Keyword(s):

Polycystic Ovary Syndrome ◽

Hormone Receptor ◽

Polycystic Ovary ◽

Receptor Gene ◽

Follicle Stimulating Hormone ◽

Sequencing Analysis ◽

Ovary Syndrome ◽

Iraqi Women ◽

Hormone Receptor Gene ◽

Stimulating Hormone

Polycystic ovary syndrome (PCOS) is a polygenic multifactorial status affecting millions of females worldwide. It is a common cause of anovulatory subfertility. Because of follicle stimulating hormone (FSH), is an important agent in human reproduction. Therefore, the correlation between follicle stimulating hormone receptor (FSHR) gene polymorphisms and polycystic ovary syndrome attracts broad attention. The objective of this study is to investigate the potential association between the follicle stimulating hormone receptor gene Thr307Ala polymorphism with polycystic ovary syndrome in the Iraqi women. A case-control study including 135 Iraqi women of Arab ethnicity (75 PCOS patients and 60 age-matched control women). The age of subjects ranged from 18 to 38 years. PCOS diagnosis was established by Rotterdam consensus criteria. The FSHR (Thr307Ala) variant was tested by conventional polymerase chain reaction (PCR)–restriction fragment length polymorphism (RFLP) followed by deoxyribonucleic acid (DNA) sequencing. The heterozygote Thr/Ala (AG) genotype of Thr307Ala (rs6165) polymorphism of follicle stimulating hormone receptor gene was giving a significant risk (odds ratio=19.4, 95%CI=1.14-30.40, P value=0.002) of developing PCOS in Iraqi women compared with control group. Sequencing analysis of DNA confirms RFLP analysis. In conclusion; the variant Thr307Ala (rs6165) of the follicle stimulating hormone receptor gene is associated with polycystic ovary syndrome and may consider as the causal factor of polycystic ovary syndrome in Iraqi women.

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