Phytochemical Investigation and Antimicrobial Activity of Root Extracts of Withania Somnifera (L.) Dunal. against Drug- Resistant Bacterial Pathogens

The study involved the selection of two isolates from Bacillus subtilis to investigate their inhibitory activity against some bacterial pathogens. B sub-bacteria were found to have a broad spectrum against test bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa. They were about 23-30 mm and less against Klebsiella sp. The sensitivity of some antibodies was tested on the test samples. The results showed that the inhibitory ability of bacterial growth in the test samples using B. subtilis extract was more effective than the antibiotics used.

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Multi-drug-resistant bacterial pathogens

American Journal of Health-System Pharmacy ◽

10.1093/ajhp/51.13.1637 ◽

1994 ◽

Vol 51 (13) ◽

pp. 1637-1637

Author(s):

C. Richard Talley

Keyword(s):

Bacterial Pathogens ◽

Drug Resistant

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PRELIMINARY PHYTOCHEMICAL AND ANTIMICROBIAL SCREENING OF UDHARA VAYUHARA CHURNA

INDIAN DRUGS ◽

10.53879/id.58.06.11787 ◽

2021 ◽

Vol 58 (06) ◽

pp. 64-67

Author(s):

Maruthi T. Ekbote ◽

◽

Rajashekar K. V ◽

Shankarappa L ◽

Bharathi D. R. ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Methanolic Extract ◽

Acorus Calamus ◽

Aqueous Extracts ◽

Piper Longum ◽

Polyherbal Formulation ◽

Methanolic Extracts ◽

Phytochemical Investigation

Ayurvedic medicines are being used increasingly to fight or prevent common diseases. Udhara vayuhara churna is a polyherbal formulation containing Piper longum (Piperceae) Zingiber officinalis (Zingiberaceae), Elettaria cardamom (Zingiberaceae), Plumbago zeylanica (Plumbaginaceae), Carum carvi (Umbelliferae), Acorus calamus (Araceae) and Embilica officinalis (Euphorbiaceae). Methanolic and aqueous extracts of Udhara vayuhara churna were screened for their antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Aspergillus niger and Candida albicans. The results of antimicrobial activity of aqueous and methanolic extracts of the Udhara vayuhara churna indicated that methanolic extract inhibited the growth of one or more test pathogens than aqueous extract. Udhara vayuhara churna formulation extracts showed a broad spectrum of antimicrobial activity. Phytochemical investigation revealed the presence of tannins, saponins, alkaloids, glycosides, flavonoids and essential oils. This may be due to the multifunctional effect of all the seven plant ingredients of Udhara Vayuhara churn.

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Comparative Study on the Antimicrobial Efficacy of Methanolic Extract of Root, Callus and Fruit Extracts of Myxopyrum smilacifolium Blume

International Journal of Applied Sciences and Biotechnology ◽

10.3126/ijasbt.v2i4.11362 ◽

2014 ◽

Vol 2 (4) ◽

pp. 521-524

Author(s):

RP Praveen ◽

Ashalantha Nair

Keyword(s):

Antimicrobial Activity ◽

Methanolic Extract ◽

Fungal Strain ◽

Antimicrobial Efficacy ◽

Bacterial Strains ◽

E Coli ◽

Root Extracts ◽

Fruit Extracts ◽

Root Callus ◽

And Staphylococcus Aureus

The aim of the present study was to compare the antimicrobial efficacy of methanolic extract of root, callus and fruit of Myxopyrum smilacifolium Blume. Antimicrobial activity was tested using agar well diffusion with four bacterial strains viz: Escherechia coli, Enterococcus faecalis, Bacillus subtilis and Staphylococcus aureus of which E. coli alone was gram negative. The fungal strain employed was Candida albicans. Root extracts shown to be effective only against B. subtilis. Fruit extracts showed the maximum antimicrobial activity against all the microbial species considered for the current study except against S. aureus. Highlight of the present study was the antimicrobial activity of callus extracts. DOI: http://dx.doi.org/10.3126/ijasbt.v2i4.11362 Int J Appl Sci Biotechnol, Vol. 2(4): 521-524

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Phytochemical investigation and in vitro antimicrobial activity of Richardia scabra

Bangladesh Journal of Pharmacology ◽

10.3329/bjp.v11i2.24666 ◽

2016 ◽

Vol 11 (2) ◽

pp. 248 ◽

Cited By ~ 1

Author(s):

Kathirvel Poonkodi ◽

Subban Ravi

Keyword(s):

Antimicrobial Activity ◽

Diffusion Method ◽

Bacterial Strains ◽

Weed Species ◽

Antibacterial And Antifungal Activities ◽

Agar Plug ◽

Phytochemical Investigation ◽

Antibacterial And Antifungal ◽

Simple Sugar

The present study was aimed to evaluate the phytochemical screening and antimicrobial activity of the petroleum ether and methanol extracts from the mature leaves of Richardia scabra from India. Disc diffusion method was used to determine the zone inhibition of the tested samples for antibacterial and agar plug method was used to determine the antifungal activity, while the microtube-dilution technique was used to determine the minimum inhibitory concentration. Both extracts showed significant antibacterial and antifungal activities when tested against 10 bacterial and four fungal strains. The minimum inhibitory concentrations of the methanol extract of R. scabra ranged between 12.5–100 μg/mL for bacterial strains. Alkaloids, steroids, flavonoids, fatty acids, terpenoids and simple sugar were detected as phytoconstituents of extracts. To the best of our knowledge, this is the first report against antimicrobial activity of common weed species R. scabra found in India.

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Antimicrobial activity of leaf and root extracts of tough lovegrass

Comunicata Scientiae ◽

10.14295/cs.v7i4.1845 ◽

2017 ◽

Vol 7 (4) ◽

pp. 420 ◽

Cited By ~ 1

Author(s):

Adriana Favaretto ◽

Fabiana Tonial ◽

Charise Dallazem Bertol ◽

Simone Meredith Scheffer-Basso

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Antifungal Activity ◽

Staphylococcus Epidermidis ◽

Phytopathogenic Fungus ◽

Leaf Extracts ◽

Reduced Pressure ◽

Root Extracts ◽

Xanthomonas Translucens ◽

Microdilution Broth

This study aimed to evaluate tough lovegrass leaf and root extracts antimicrobial activity. The extracts (plant material: solvent, 1:10) were prepared by maceration with methanol:water (1:1) during ten days followed by a concentration in a rotary evaporator under reduced pressure. The extracts were resuspended in water containing 1% of dimethylsulfoxide (DMSO) to obtain a final concentration of 100 mg/mL and then filtered through a sterilizing membrane with 0.22μm. The antibacterial activity of the leaf and root extracts were evaluated against pathogenic and phytopathogenic bacteria by agar well diffusion and microdilution broth methods for the minimum inhibitory concentrations (MIC) determination. The antifungal activity of tough lovegrass leaf and root extracts were evaluated by micelial growth inhibition and conidial germination inhibition. The extracts presented low antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, and Xanthomonas translucens, but the leaf extracts presented significant antifungal activity against the phytopathogenic fungus Drechslera tritici-repentis. The results support the continuity of the study in improving the effectiveness of the active extract for a possible use in pharmacology and agronomy and in attempting to determine the probable active antimicrobial compound.

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Withania somnifera acts as a potential insect growth regulator in the polyphagous pest, Pericallia ricini

Journal of Plant Protection Research ◽

10.1515/jppr-2017-0052 ◽

2018 ◽

Vol 0 (0) ◽

Cited By ~ 1

Author(s):

Sandeep Kumar Gaur ◽

Krishna Kumar

Keyword(s):

Medicinal Plant ◽

Withania Somnifera ◽

Endocrine System ◽

Adult Emergence ◽

Topical Administration ◽

Insect Growth Regulator ◽

Developmental Abnormalities ◽

Insect Growth ◽

Root Extracts ◽

Seed Extracts

Abstract Both seed and root extracts of the medicinal plant, Ashwagandha, Withania somnifera exhibit insect growth regulatory activity against the polyphagous pest, Pericallia ricini. Topical administration of W. somnifera seed and root extracts to last instar larvae of P. ricini disrupted moulting and metamorphosis, leading to a number of developmental abnormalities such as delay in larval-pupal and pupal-adult ecdysis, formation of larval-pupal, pupal-adult and larval-pupal-adult mosaics/chimeras, ecdysial failure, suppression of pupation and adult emergence and formation of abnormal pupae and adultoids. The treatment with seed extracts was more severe than that of root extracts as it completely suppressed the pupation and adult emergence. The results clearly suggest that the medicinal plant, W. somnifera acts as a potential insect growth regulatory (IGR) disrupting the moulting and metamorphosis as a consequence of interference with the endocrine system.

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