3D printing of mineral–polymer bone substitutes based on sodium alginate and calcium phosphate

We demonstrate a relatively simple route for three-dimensional (3D) printing of complex-shaped biocompatible structures based on sodium alginate and calcium phosphate (CP) for bone tissue engineering. The fabrication of 3D composite structures was performed through the synthesis of inorganic particles within a biopolymer macromolecular network during 3D printing process. The formation of a new CP phase was studied through X-ray diffraction, Fourier transform infrared spectroscopy and scanning electron microscopy. Both the phase composition and the diameter of the CP particles depend on the concentration of a liquid component (i.e., the “ink”). The 3D printed structures were fabricated and found to have large interconnected porous systems (mean diameter ≈800 μm) and were found to possess compressive strengths from 0.45 to 1.0 MPa. This new approach can be effectively applied for fabrication of biocompatible scaffolds for bone tissue engineering constructions.

Download Full-text

3D Printing of Bone‐Mimetic Scaffold Composed of Gelatin/β‐Tri‐Calcium Phosphate for Bone Tissue Engineering

Macromolecular Bioscience ◽

10.1002/mabi.202000256 ◽

2020 ◽

Vol 20 (12) ◽

pp. 2000256

Author(s):

Jae Eun Jeong ◽

Shin Young Park ◽

Ji Youn Shin ◽

Ji Min Seok ◽

June Ho Byun ◽

...

Keyword(s):

Tissue Engineering ◽

Calcium Phosphate ◽

3D Printing ◽

Bone Tissue Engineering ◽

Bone Tissue

Download Full-text

3D Printing of Calcium Phosphate Ceramics for Bone Tissue Engineering and Drug Delivery

Annals of Biomedical Engineering ◽

10.1007/s10439-016-1678-3 ◽

2016 ◽

Vol 45 (1) ◽

pp. 23-44 ◽

Cited By ~ 135

Author(s):

Ryan Trombetta ◽

Jason A. Inzana ◽

Edward M. Schwarz ◽

Stephen L. Kates ◽

Hani A. Awad

Keyword(s):

Drug Delivery ◽

Tissue Engineering ◽

Calcium Phosphate ◽

3D Printing ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Calcium Phosphate Ceramics

Download Full-text

3D-printing of porous calcium phosphate cements for bone tissue engineering

Dental Materials ◽

10.1016/j.dental.2016.08.116 ◽

2016 ◽

Vol 32 ◽

pp. e56-e57 ◽

Cited By ~ 8

Author(s):

K. Khoshroo ◽

T. Almela ◽

M. Tahriri ◽

F. Fahimipour ◽

Z. Metalwala ◽

...

Keyword(s):

Tissue Engineering ◽

Calcium Phosphate ◽

3D Printing ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Calcium Phosphate Cements

Download Full-text

Pro-angiogenic and osteogenic composite scaffolds of fibrin, alginate and calcium phosphate for bone tissue engineering

Journal of Tissue Engineering ◽

10.1177/20417314211005610 ◽

2021 ◽

Vol 12 ◽

pp. 204173142110056

Author(s):

Nupur Kohli ◽

Vaibhav Sharma ◽

Alodia Orera ◽

Prasad Sawadkar ◽

Nazanin Owji ◽

...

Keyword(s):

Tissue Engineering ◽

Calcium Phosphate ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Chorioallantoic Membrane ◽

Cam Assay ◽

Clinical Model ◽

Chick Chorioallantoic Membrane ◽

Biomimetic Method ◽

Alginate Scaffold

Due to the limitations of bone autografts, we aimed to develop new composite biomaterials with pro-angiogenic and osteogenic properties to be used as scaffolds in bone tissue engineering applications. We used a porous, cross-linked and slowly biodegradable fibrin/alginate scaffold originally developed in our laboratory for wound healing, throughout which deposits of calcium phosphate (CaP) were evenly incorporated using an established biomimetic method. Material characterisation revealed the porous nature and confirmed the deposition of CaP precursor phases throughout the scaffolds. MC3T3-E1 cells adhered to the scaffolds, proliferated, migrated and differentiated down the osteogenic pathway during the culture period. Chick chorioallantoic membrane (CAM) assay results showed that the scaffolds were pro-angiogenic and biocompatible. The work presented here gave useful insights into the potential of these pro-angiogenic and osteogenic scaffolds for bone tissue engineering and merits further research in a pre-clinical model prior to its clinical translation.

Download Full-text

Three-dimensionally printed polycaprolactone/multicomponent bioactive glass scaffolds for potential application in bone tissue engineering

Biomedical glasses ◽

10.1515/bglass-2020-0006 ◽

2020 ◽

Vol 6 (1) ◽

pp. 57-69

Author(s):

Amirhosein Fathi ◽

Farzad Kermani ◽

Aliasghar Behnamghader ◽

Sara Banijamali ◽

Masoud Mozafari ◽

...

Keyword(s):

Tissue Engineering ◽

3D Printing ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Three Dimensional ◽

Layer By Layer ◽

Composite Scaffolds ◽

3D Printed ◽

Co Doped

AbstractOver the last years, three-dimensional (3D) printing has been successfully applied to produce suitable substitutes for treating bone defects. In this work, 3D printed composite scaffolds of polycaprolactone (PCL) and strontium (Sr)- and cobalt (Co)-doped multi-component melt-derived bioactive glasses (BGs) were prepared for bone tissue engineering strategies. For this purpose, 30% of as-prepared BG particles (size <38 μm) were incorporated into PCL, and then the obtained composite mix was introduced into a 3D printing machine to fabricate layer-by-layer porous structures with the size of 12 × 12 × 2 mm3.The scaffolds were fully characterized through a series of physico-chemical and biological assays. Adding the BGs to PCL led to an improvement in the compressive strength of the fabricated scaffolds and increased their hydrophilicity. Furthermore, the PCL/BG scaffolds showed apatite-forming ability (i.e., bioactivity behavior) after being immersed in simulated body fluid (SBF). The in vitro cellular examinations revealed the cytocompatibility of the scaffolds and confirmed them as suitable substrates for the adhesion and proliferation of MG-63 osteosarcoma cells. In conclusion, 3D printed composite scaffolds made of PCL and Sr- and Co-doped BGs might be potentially-beneficial bone replacements, and the achieved results motivate further research on these materials.

Download Full-text

Preparation and characterization of nano biphasic calcium phosphate/poly-L-lactide composite scaffold

Science and Engineering of Composite Materials ◽

10.1515/secm-2014-0100 ◽

2016 ◽

Vol 23 (1) ◽

pp. 37-44 ◽

Cited By ~ 1

Author(s):

Weizhong Yang ◽

Yong Yi ◽

Yuan Ma ◽

Li Zhang ◽

Jianwen Gu ◽

...

Keyword(s):

Tissue Engineering ◽

Calcium Phosphate ◽

Cell Viability ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Biphasic Calcium Phosphate ◽

Composite Scaffold ◽

Pore Wall ◽

Tissue Engineering Scaffold ◽

Plla Scaffold

AbstractNano biphasic calcium phosphate (BCP) particles were synthesized using the sol-gel method. As-prepared BCP particles were combined with poly-L-lactide (PLLA) to fabricate nano-BCP/PLLA composite scaffold through a series of processing steps containing solvent self-diffusion, hot-pressing, and particulate leaching. The composite had a suitable porous structure for bone tissue engineering scaffold. In comparison, micro-BCP/PLLA scaffold was studied as well. Nano-BCP particles were distributed homogeneously in the PLLA matrix, and much more tiny crystallites exposed on the surface of the pore wall. Due to the finer inorganic particle distribution in the PLLA phase and the larger area of the bioactive phase exposed in the pore wall surface, nano-BCP/PLLA scaffold had enhanced compressive strength, good bioactivity, and superior cell viability. A nonstoichiometric apatite layer could be rapidly formed on the surface of nano- BCP/PLLA when soaked in simulated body fluid. The MG-63 cell viability of nano-BCP/PLLA scaffold is significantly higher than that of micro-BCP/PLLA scaffold. Therefore, nano-BCP/PLLA composite may be a suitable alternative for bone tissue engineering scaffold.

Download Full-text