DEVELOPMENT OF NANOSTRUCTURED BIOPLASTIC MATERIAL FOR COMBUSTIOLOGY

2020 ◽  
pp. 45-48
Author(s):  
P. Eremin ◽  
Y. Kostromina ◽  
R. Yakupova
Keyword(s):  
Cell Proliferation ◽  
Hyaluronic Acid ◽  
Clinical Practice ◽  
Physical Properties ◽  
Culture Medium ◽  
Human Fibroblasts ◽  
Structure And Properties ◽  
Cytotoxic Effects ◽  
Good Cell ◽  
Effective Conditions

The technique of obtaining a new bioplastic material based on collagen, elastin and hyaluronic acid is described. The results of a study of the biomaterial structure and properties in order to assess the prospects for its further use in clinical practice are presented. Co-culturing of the bioplastic material and human fibroblasts did not reveal any its cytotoxic effects on cells in culture. It was shown that the biomaterial samples were able to maintain physical properties in the culture medium for more than 10 days. Due to its physical properties and structure, the use of created biomaterial provides effective conditions for good cell proliferation, which allows us to consider it as a promising biomaterial for use in clinical practice.

scholarly journals Development of nanostructured bioplastic material for wound healing

2021 ◽  
Author(s):  
Ilmira R. Gilmutdinova ◽  
Elena Kostromina ◽  
Regina D. Yakupova ◽  
Petr S. Eremin
Keyword(s):  
Hyaluronic Acid ◽  
Clinical Practice ◽  
Physical Properties ◽  
Human Fibroblasts ◽  
Fibroblast Cell ◽  
Human Skin Fibroblast ◽  
High Porosity ◽  
The Matrix ◽  
Photochemical Crosslinking ◽  
Fibroblast Cell Culture

The development of new biomaterials whose characteristics are as close as possible to the properties of living human tissues is one of the most promising areas of regenerative medicine. This work aimed at creating a bioplastic material based on collagen, elastin and hyaluronic acid and studying its structure and properties to assess the prospects for further use in clinical practice. Bioplastic material was obtained by mixing collagen, hyaluronic acid and elastin in predetermined proportions with distilled water. We treated the material with photochemical crosslinking to stabilize biofilm in a liquid medium and form a nanostructured scaffold. A commercial human skin fibroblast cell culture was used to assess the biomaterial cytotoxicity and biocompatibility. The visualization and studies of the biomaterial structure were performed using light and scanning electron microscopy. It has been shown that the obtained biomaterial is characterized by high resilience; it has also a high porosity. The co-culturing of the bioplastic material and human fibroblasts did not reveal any of its cytotoxic effects on cells in culture. It was shown that the biomaterial samples could maintain physical properties in the culture medium for more than 10 days, while the destruction of the matrix was observed 3–4 weeks after the beginning of incubation. Thus, the created biomaterial can be used on damaged skin areas due to its physical properties and structure. The use of the developed biomaterial provides effective conditions for good cell proliferation, which allows us to consider it as a promising wound cover for use in clinical practice.

scholarly journals Development of nanostructured bioplastic material for wound healing

2021 ◽  
Author(s):  
Ilmira R. Gilmutdinova ◽  
Elena Kostromina ◽  
Regina D. Yakupova ◽  
Petr S. Eremin
Keyword(s):  
Hyaluronic Acid ◽  
Clinical Practice ◽  
Physical Properties ◽  
Human Fibroblasts ◽  
Fibroblast Cell ◽  
Human Skin Fibroblast ◽  
High Porosity ◽  
The Matrix ◽  
Photochemical Crosslinking ◽  
Fibroblast Cell Culture

The development of new biomaterials whose characteristics are as close as possible to the properties of living human tissues is one of the most promising areas of regenerative medicine. This work aimed at creating a bioplastic material based on collagen, elastin and hyaluronic acid and studying its structure and properties to assess the prospects for further use in clinical practice. Bioplastic material was obtained by mixing collagen, hyaluronic acid and elastin in predetermined proportions with distilled water. We treated the material with photochemical crosslinking to stabilize biofilm in a liquid medium and form a nanostructured scaffold. A commercial human skin fibroblast cell culture was used to assess the biomaterial cytotoxicity and biocompatibility. The visualization and studies of the biomaterial structure were performed using light and scanning electron microscopy. It has been shown that the obtained biomaterial is characterized by high resilience; it has also a high porosity. The co-culturing of the bioplastic material and human fibroblasts did not reveal any of its cytotoxic effects on cells in culture. It was shown that the biomaterial samples could maintain physical properties in the culture medium for more than 10 days, while the destruction of the matrix was observed 3–4 weeks after the beginning of incubation. Thus, the created biomaterial can be used on damaged skin areas due to its physical properties and structure. The use of the developed biomaterial provides effective conditions for good cell proliferation, which allows us to consider it as a promising wound cover for use in clinical practice.

Creation of a Novel Nanostructured Bioplastic Material for Combustiology

2020 ◽  
Vol 36 (4) ◽  
pp. 65-68
Author(s):  
E.Yu. Kostromina ◽  
R.D. Yakupova ◽  
P.S. Eremin
Keyword(s):  
Hyaluronic Acid ◽  
Clinical Practice ◽  
Physical Properties ◽  
Human Fibroblasts ◽  
Fibroblast Cell ◽  
Human Skin Fibroblast ◽  
High Porosity ◽  
The Matrix ◽  
New Material ◽  
Fibroblast Cell Culture

The technique of obtainment of a new bioplastic material based on collagen, elastin and hyaluronic acid is described. The results of a study of the biomaterial structure and properties to assess the prospects for its further use in clinical practice are also presented. To prepare the material samples, collagen, elastin and hyaluronic acid were mixed in predetermined proportions with distilled water, and the resulting biofilm was crosslinked by UV irradiation. A commercial human skin fibroblast cell culture (HDF) was used to assess the biomaterial cytotoxicity and biocompatibility; as a result, it was shown that cytotoxicity is absent in it. The visualization and studies of the biomaterial structure were carried out using light microscopy. The new material was shown to be highly elastic and resilient; it also had a high porosity with a pore diameter of 100-200 um. It was shown that the biomaterial samples were able to maintain physical properties in the culture medium for more than 10 days, while the complete destruction of the matrix was observed 3-4 weeks after the beginning of incubation. Due to its physical properties and structure, and also the capacity of providing effective conditions for good cell proliferation, the created biomaterial can be used as a wound cover in the damaged skin areas. This allows us to consider the new biomaterial promising for clinical practice. cell technologies, tissue-engineered constructs, bioplastic material, collagen, human fibroblasts.

Analysis of efficiency of application of media with hyaluronic acid in cryoprotocols

GYNECOLOGY ◽  
2020 ◽  
Vol 22 (2) ◽  
pp. 26-29
Author(s):  
Natalia V. Protopopova ◽  
Elena B. Druzhinina ◽  
Kseniia V. Krylova ◽  
Iuliia V. Mylnikova ◽  
Jan A. Dvoryanov ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Culture Medium ◽  
Genetic Diagnosis ◽  
World Health ◽  
Practical Significance ◽  
Pelvic Surgery ◽  
High Concentration ◽  
Tubal Infertility

According to the World Health Organization, about 2 million new couples experience infertility annually, and their number is growing. An effective way to overcome infertility is assisted reproductive technology (ART). Cryopreservation will rationally solve the issue of preservation and further use of embryos: to delay pregnancy for some time considering womans desire and to prevent ovarian hyperstimulation syndrome. Embryo freezing allows to reduce the rate of repeated ovarian stimulation and perform preimplantation genetic diagnosis. Over the past decades, various cryotransfer options have been proposed to increase ART treatment efficacy, including the use of a culture medium with a high concentration of hyaluronic acid, but there are conflicting data on the use of such a medium in ART programs. Aim. Evaluation of thawed embryo transfers efficacy using the hyaluronic acid-containing culture medium. To achieve the goal, the following tasks were set: to evaluate clinical and medical history data of patients with tubal infertility in cryoprotocols, to analyze the previous cycle of in vitro fertilization and embryological stage, to evaluate the effectiveness of the culture medium with a high content of hyaluronic acid. Materials and methods. A detailed description of the patient sample, inclusion and exclusion criteria, embryological stage, embryo grading, devitrified embryo transfer technique. The article includes 3 tables which present the groups general clinical characteristics, the embryological stage, the rate of pregnancy, depending on the cultivation day. Results. The authors established that in patients with a history of pelvic surgery and sexually transmitted infections, it is advisable to use the culture medium with a high content of hyaluronic acid to transfer the thawed embryo. It was shown that pregnancy rate is 1.5 times higher when transferring devitrified embryos on the 5th day of development with the use of hyaluronic acid-containing culture medium. The conclusion about the pregnancy rate in obese patients is not indisputable, which requires further study. The authors also provide practical recommendations on the use of the culture medium with hyaluronic acid in cryoprotocols. Conclusion. The study allows to optimize the devitrified embryo transfer in patients with tubal infertility using a culture medium with a high content of hyaluronan. This work has undoubted scientific and practical significance.

Mechanism of cystine reaccumulation by cystinotic fibroblasts in vitro

1990 ◽  
Vol 10 (2) ◽  
pp. 225-229 ◽  
Author(s):  
Susan Forster ◽  
Lynne Scarlett ◽  
John B. Lloyd
Keyword(s):  
Plasma Membrane ◽  
Culture Medium ◽  
Human Fibroblasts ◽  
Competitive Inhibitor ◽  
Free Medium ◽  
Lysosome Membrane ◽  
Putative Mechanism

It is well established that when cystine-depleted cystinotic cells are cultured in cystine-containing medium, they reaccumulate cystine within their lysosomes more rapidly than when cultured in cystine-free medium. This has been a puzzling result, since the lysosome membrane of cystinotic cells is impermeable to cystine. To probe the mechanism of cystine reaccumulation, we have measured reaccumulation in the presence of colchicine, an inhibitor of pinocytosis, or of glutamate, a competitive inhibitor of cystine transport into human fibroblasts. Colchicine had no effect, thus eliminating pinocytosis as a putative mechanism for cystine translocation from the culture medium to the lysosomes. Glutamate, however, strongly inhibited cystine reaccumulation. It is concluded that the true mechanism is as follows. 1. Exogenous cystine crosses the plasma membrane on the cystine-glutamate porter. 2. Cystine is reduced in the cytoplasm by GSH. 3. The cysteine that is generated enters the lysosome, where it becomes cystine by participating in the reduction of cystine residues during intralysosomal proteolysis, or by autoxidation.

scholarly journals Effect of Lignocaine Concentration on Human Fibroblasts Growth in Eyes Undergoing Trabeculectomy: An in vitro Study

2018 ◽  
Vol 3 (3) ◽  
pp. 1-10 ◽  
Author(s):  
Madhuravasal Krishnan Janani ◽  
Venkatakrishnan Jaichandran ◽  
Hajib Narahari Rao Madhavan ◽  
Lingam Vijaya ◽  
Ronnie Jacob George ◽  
...  
Keyword(s):  
Culture Medium ◽  
In Vitro Model ◽  
Morphological Changes ◽  
Human Fibroblasts ◽  
Annexin V ◽  
In Vitro Study ◽  
Tenon’S Capsule ◽  
Tenon's Capsule ◽  
Vitro Model

Purpose: To evaluate the effect of lignocaine on growth and apoptosis indication of primary human Tenon’s capsule fibroblast (HTFs) in an in vitro model. Patients and Methods: Tenon’s capsule tissue obtained from patients undergoing trabeculectomy were grown in cell culture medium. The effect of different concentrations of lignocaine (0.5, 1.0, 1.5, and 2%) on the morphology and growth of the fibroblasts was studied using microscopy, cell viability, and proliferation assay, and apoptosis was detected using the FITC Annexin V Apoptosis Kit. Results: Morphological changes similar to those of apoptotic cells, including cytoplasmic vacuolation, shrinkage, and rounding were visualized in the cells treated with concentrations greater than 1.0% (i.e., 1.5, 2.0%). Though proliferation inhibition was found with all four concentrations (0.5–2.0%), the viability of cells decreased from 1.0% lignocaine. Conclusion: 0.5% lignocaine prevents proliferation of fibroblasts without causing apoptosis in vitro.

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