Low-Level Atrazine Exposure Decreases Cell Proliferation in Human Fibroblasts

2004 ◽  
Vol 46 (4) ◽  
Author(s):  
Michelle K. Manske ◽  
Lisa A. Beltz ◽  
Kavita R. Dhanwada
Keyword(s):  
Cell Proliferation ◽  
Human Fibroblasts ◽  
Low Level

scholarly journals Hyaluronate synthetase inhibition by normal and transformed human fibroblasts during growth reduction

1987 ◽  
Vol 104 (4) ◽  
pp. 1105-1115 ◽  
Author(s):  
K Matuoka ◽  
M Namba ◽  
Y Mitsui
Keyword(s):  
Cell Proliferation ◽  
Cell Density ◽  
Human Fibroblasts ◽  
Transformed Cells ◽  
Synthetase Activity ◽  
Intact Cells ◽  
Free System ◽  
Glycosaminoglycan Synthesis ◽  
Cell Free System ◽  
A Cell

To establish the relation of glycosaminoglycan synthesis to cell proliferation, we investigated the synthesis of individual glycosaminoglycan species by intact cells and in a cell-free system, using normal and transformed human fibroblasts under differing culture conditions. Reducing serum concentration brought about a marked decline in the synthesis of hyaluronate (HA) as well as cell proliferation on both normal and transformed cells. Both HA synthesis and proliferation decreased with increasing cell densities markedly (in inverse proportion to cell density) in normal cells but gradually in transformed cells. This noticeable congruity of the changes in HA synthesis and proliferation indicates that the change in HA synthesis is related primarily to cell proliferation rather than to cell density or cellular transformation. Examination of HA synthesis in a cell-free system demonstrated that the activity of HA synthetase also fluctuated in conjunction with cell proliferation. Furthermore, growth-reduced cells (except crowded transformed cells) inhibited cell-free HA synthesis and this inhibition was induced coincidentally with a decrease in both HA synthetase activity and proliferation. These findings suggest that the change in HA synthesis is significant in the regulation of cell proliferation.

Cell proliferation of human fibroblasts and osteoblasts in osteogenesis imperfecta: Influence of age

2009 ◽  
Vol 10 (11) ◽  
pp. 1705-1712 ◽  
Author(s):  
N.S. Fedarko ◽  
P. D'Avis ◽  
C.R. Frazier ◽  
M.J. Burrill ◽  
V. Fergusson ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Osteogenesis Imperfecta ◽  
Human Fibroblasts

High-throughput live-cell imaging reveals differential inhibition of tumor cell proliferation by human fibroblasts

2010 ◽  
Vol 128 (12) ◽  
pp. 2793-2802 ◽  
Author(s):  
Emilie Flaberg ◽  
Laszlo Markasz ◽  
Gabor Petranyi ◽  
Gyorgy Stuber ◽  
Ferenc Dicső ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Tumor Cell ◽  
High Throughput ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Human Fibroblasts ◽  
Live Cell ◽  
Tumor Cell Proliferation ◽  
Differential Inhibition

scholarly journals Low-level laser irradiation promotes proliferation of cryopreserved adipose-derived stem cells

2017 ◽  
Vol 15 (3) ◽  
pp. 334-338 ◽  
Author(s):  
Fernanda Ginani ◽  
Diego Moura Soares ◽  
Hugo Alexandre de Oliveira Rocha ◽  
Carlos Augusto Galvão Barboza
Keyword(s):  
Stem Cells ◽  
Flow Cytometry ◽  
Cell Proliferation ◽  
Laser Irradiation ◽  
Adipose Derived Stem Cells ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Significant Difference ◽  
Low Level Laser Irradiation

ABSTRACT Objective To evaluate the effect of low-level laser irradiation on proliferation and viability of murine adipose-derived stem cells previously submitted to cryopreservation. Methods Adipose-derived stem cells were isolated from inguinal fat pads of three mice, submitted to cryopreservation in fetal bovine serum with 10% dimethylsulfoxide for 30 days and then thawed and maintained in normal culture conditions. Culture cells were either irradiated or not (control) with an InGaAIP diode laser at zero and 48 hours, using two different energy densities (0.5 and 1.0J/cm2). Cell proliferation was evaluated by trypan blue exclusion method and MTT assay at intervals of zero, 24, 48, and 72 hours after the first laser application. Cell viability and apoptosis of previously cryopreserved cells submitted to laser therapy were evaluated by flow cytometry. Results The Irradiated Groups (0.5 and 1.0J/cm2) showed an increased cell proliferation (p<0.05) when compared to the Control Group, however no significant difference between the two energy densities was observed. Flow cytometry revealed a percentage of viable cells higher than 99% in all groups. Conclusion Low-level laser irradiation has stimulatory effects on the proliferation of adipose-derived stem cells previously submitted to cryopreservation.

scholarly journals OxLDL stimulates cell proliferation through a general induction of cell cycle proteins

2003 ◽  
Vol 284 (2) ◽  
pp. H644-H653 ◽  
Author(s):  
Marjorie E. Zettler ◽  
Michele A. Prociuk ◽  
J. Alejandro Austria ◽  
Hamid Massaeli ◽  
Guangming Zhong ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Low Density Lipoprotein ◽  
Human Fibroblasts ◽  
Density Lipoprotein ◽  
Cyclin B1 ◽  
Cyclin Dependent Kinase ◽  
Cell Cycle Proteins ◽  
Cell Counts ◽  
Cell Cycle Inhibitors

Oxidized low-density lipoprotein (oxLDL) may be involved in atherosclerosis by stimulating proliferation of cells in the vessel wall. The purpose of this study was to identify the mechanism by which oxLDL induces proliferation. Quiescent human fibroblasts and rabbit smooth muscle cells were treated with 0, 10, or 50 μg/ml oxLDL for 24–48 h. This resulted in significant increases in total cell counts at both concentrations of oxLDL, at both time points, for both types of cells. Western blot analysis revealed that oxLDL-stimulated cell proliferation was associated with significant increases in the expression of proteins that regulate entry into and progression through the cell cycle [cell division cycle 2, cyclin-dependent kinase (cdk) 2, cdk 4, cyclin B1, cyclin D1, and PCNA]. Surprisingly, the expression of cell cycle inhibitors (p21 and p27) was stimulated by oxLDL as well, but this was to a lesser extent than the effects on cell cycle-activating proteins. OxLDL also induced nuclear localization of all cell cycle proteins examined. The similar effects of oxLDL on the translocation and expression of both cell cycle-activating and -inhibiting proteins may explain the controlled proliferative phenomenon observed in atherosclerosis as opposed to the more rapid proliferative event characteristic of cancer.

scholarly journals Mel-18, a Polycomb Group Protein, Regulates Cell Proliferation and Senescence via Transcriptional Repression of Bmi-1 and c-Myc Oncoproteins

2007 ◽  
Vol 18 (2) ◽  
pp. 536-546 ◽  
Author(s):  
Wei-Jian Guo ◽  
Sonal Datta ◽  
Vimla Band ◽  
Goberdhan P. Dimri
Keyword(s):  
Cell Proliferation ◽  
Transcriptional Repression ◽  
Target Genes ◽  
Human Fibroblasts ◽  
Ring Finger ◽  
Polycomb Group ◽  
Polycomb Group Protein ◽  
Down Regulation ◽  
Group Protein ◽  
Bona Fide

Polycomb group (PcG) protein Bmi-1 is an important regulator of cell proliferation. It regulates cellular senescence and proliferation of cells via the transcriptional repression of INK4a/ARF locus and other target genes. Here, we report that Mel-18, a PcG ring finger protein (PCGF) transcriptionally down-regulates Bmi-1. Furthermore, the expression of Bmi-1 and Mel-18 inversely correlates in proliferating and senescent human fibroblasts. Bmi-1 down-regulation by Mel-18 results in accelerated senescence and shortening of the replicative life span in normal human cells. Importantly, using promoter-reporter, chromatin immunoprecipitation, and quantitative real-time primary transcript RT-PCR assays, and an RNA interference approach, we demonstrate that Bmi-1 is a bona fide target of c-Myc oncoprotein. Finally, our data suggest that Mel-18 regulates Bmi-1 expression during senescence via down-regulation of c-Myc. These studies link c-Myc and polycomb function in cell proliferation and senescence.

Aluminum Ions Induce DNA Synthesis But Not Cell Proliferation in Human Fibroblasts In Vitro

2002 ◽  
Vol 86 (1) ◽  
pp. 01-10 ◽  
Author(s):  
Carmen Domínguez ◽  
Antonio Moreno ◽  
Marc Llovera
Keyword(s):  
Cell Proliferation ◽  
Dna Synthesis ◽  
Human Fibroblasts ◽  
Aluminum Ions
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