scholarly journals On-Chip PCR Based Plasmonic Microfluidic Platform: Ultrafast Point-of-Care Diagnostics of SARS-CoV-2

2021 ◽  
Vol 3 (1) ◽  
pp. 5-11
Author(s):  
Megha Agrawal ◽  
Keyword(s):  
Small Volume ◽  
Point Of Care ◽  
Rapid Screening ◽  
Viral Diseases ◽  
Chain Reaction ◽  
Point Of Care Diagnostics ◽  
Screening And Identification ◽  
Polymerase Chain ◽  
Diagnostic Applications ◽  
On Chip

It is critically important to have rapid screening and identification of contagious viral diseases such as the current COVID-19 pandemic that is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Rapid and accurate diagnostic is essential for preventing worldwide spread of virus and ensuring in-time care for patients during the fast spread of pandemic diseases. Nanobiotechnology enabled tools have allowed to develop advanced polymerase chain reaction (PCR) based diagnostics of contagious viral diseases. To this end, microfluidic on-chip PCR platforms have shown huge promise for highly efficient, rapid and small-volume bioassay for point-of-care (POC) diagnostic applications in mitigating the challenges of SARS-CoV-2. Here, we discuss latest advances in ultrafast, real-time, and on-chip nanoplasmonic PCR for rapid and quantitative molecular diagnostics at POC level.

An integrated CMOS quantitative-polymerase-chain-reaction lab-on-chip for point-of-care diagnostics

Lab on a Chip ◽  
2014 ◽  
Vol 14 (20) ◽  
pp. 4076-4084 ◽  
Author(s):  
Haig Norian ◽  
Ryan M. Field ◽  
Ioannis Kymissis ◽  
Kenneth L. Shepard
Keyword(s):  
Integrated Circuit ◽  
Point Of Care ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Lab On Chip ◽  
Cmos Integrated Circuit ◽  
Point Of Care Diagnostics ◽  
Droplet Transport ◽  
Polymerase Chain ◽  
On Chip

qPCR demonstrated on the surface of a CMOS integrated circuit using embedded heaters, temperature sensors, photodiodes, and electrowetting-based droplet transport mechanism.

Multiplex reverse transcription-polymerase chain reaction combined with on-chip electrophoresis as a rapid screening tool for candidate gene sets

2005 ◽  
Vol 26 (9) ◽  
pp. 1687-1691 ◽  
Author(s):  
Rainer Wittig ◽  
Rüdiger Salowsky ◽  
Stephanie Blaich ◽  
Stefan Lyer ◽  
Juehn S. Maa ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Candidate Gene ◽  
Reverse Transcription ◽  
Screening Tool ◽  
Rapid Screening ◽  
Chain Reaction ◽  
Gene Sets ◽  
Polymerase Chain ◽  
On Chip

Direct urine polymerase chain reaction for chlamydia and gonorrhoea: a simple means of bringing high-throughput rapid testing to remote settings?

Sexual Health ◽  
2013 ◽  
Vol 10 (4) ◽  
pp. 299 ◽  
Author(s):  
Frashta Rahimi ◽  
Namraj Goire ◽  
Rebecca Guy ◽  
John M. Kaldor ◽  
James Ward ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
High Throughput ◽  
Real Time Pcr ◽  
Point Of Care ◽  
Resistance Mechanisms ◽  
Rapid Screening ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Urine Testing

Background Rapid point-of-care tests (POCTs) for chlamydia (Chlamydia trachomatis) and gonorrhoea (Neisseria gonorrhoeae) have the potential to confer health benefits in certain populations even at moderate sensitivities; however, suitable POCTs for these organisms are currently lacking. Methods: In this study, we investigated the use of direct urine polymerase chain reaction (PCR), with the view of implementing a simplified PCR strategy for high-throughput chlamydia and gonorrhoea screening in remote settings. Briefly, a simple dilution of the urine was performed before adding it directly to a real-time PCR reaction. The method was evaluated using 134 stored urine specimens that had been submitted for chlamydia and gonorrhoea testing and had been tested using a commercial C. trachomatis and N. gonorrhoeae PCR method. These included samples that were PCR-positive for chlamydia (n = 87), gonorrhoea (n = 16) or both (n = 2). Direct urine testing was conducted using previously described in-house real-time PCR methods for C. trachomatis and N. gonorrhoeae as well as for recognised N.gonorrhoeae antimicrobial resistance mechanisms. Results: The overall sensitivities and specificities of the direct urine PCR were 78% and 100% for chlamydia, and 83% and 100% for gonorrhoea. N.gonorrhoeae penicillin and quinolone resistance mechanisms were characterised in 14 of the 18 N. gonorrhoeae-positive samples. Conclusions: The results of this study show that the simplified PCR strategy may be a feasible approach for rapid screening and improving chlamydia and gonorrhoea treatment in remote settings.

scholarly journals Evaluation of commercially available immuno-magnetic agglutination and enzyme-linked immunosorbent assays for rapid point-of-care diagnostics of COVID-19

2020 ◽  
Author(s):  
Maria Engel Moeller ◽  
Jeppe Fock ◽  
Pearlyn Pah ◽  
Antia De La Campa Veras ◽  
Melanie Bade ◽  
...  
Keyword(s):  
Point Of Care ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plasma Samples ◽  
Igg Antibodies ◽  
Chain Reaction ◽  
Point Of Care Test ◽  
Point Of Care Diagnostics ◽  
Polymerase Chain ◽  
Respiratory Coronavirus ◽  
Immunomagnetic Assay

Introduction: Coronavirus Disease 2019 (COVID-19) is caused by severe acute respiratory coronavirus-2 (SARS-CoV-2). Fast, accurate and simple blood-based assays for quantification of anti-SARS-CoV-2 antibodies are urgently needed to identify infected individuals and keep track of the spread of disease. Methods: The study included 35 plasma samples from 22 individuals with confirmed COVID-19 by real time reverse transcriptase polymerase chain reaction and 40 non COVID-19 plasma samples. Anti-SARS-CoV-2 IgM/IgA or IgG antibodies were detected by a microfluidic quantitative immunomagnetic assay (IMA)(ViroTrack Sero COVID IgM+IgA/IgG Ab, Blusense Diagnostics, Denmark) and by enzyme-linked immunosorbent assay ((ELISA) (EuroImmun Medizinische Labordiagnostika, Germany). Results: Of the 35 plasma samples from the COVID-19 patients, 29 (82.9%) were positive for IgA/IgM or IgG by IMA and 29 samples (82.9%) were positive by ELISA. Sensitivity for only one sample per patient was 68% for IgA+IgM and 73% IgG by IMA and 73% by ELISA. For samples collected 14 days after symptom onset, the sensitivity of both IMA and ELISA was around 90%. Specificity of the IMA reached 100% compared to 95% for ELISA IgA and 97.5% for ELISA IgG. Conclusion: IMA for COVID-19 is a rapid simple-to-use point of care test with sensitivity and specificity similar to a commercial ELISA.

Plasmonic and label-free real-time quantitative PCR for point-of-care diagnostics

The Analyst ◽  
2021 ◽  
Author(s):  
Padideh Mohammadyousef ◽  
Miltiadis Paliouras ◽  
Mark Trifiro ◽  
Andrew Kirk
Keyword(s):  
Pathogen Detection ◽  
Point Of Care ◽  
Medical Community ◽  
Molecular Diagnostic ◽  
Label Free ◽  
Chain Reaction ◽  
Real Time Quantitative Pcr ◽  
Point Of Care Diagnostics ◽  
Polymerase Chain ◽  
Infectious Pathogen

In response to the world’s medical community need for accurate and immediate infectious pathogen detection, many researchers have focused on adapting the standard molecular diagnostic method of polymerase chain reaction...

scholarly journals A Self-Contained Portable Polymerase-Chain-Reaction System Integrated with Electromagnetic Mini-Actuators for Bi-Directional Fluid Transport

2011 ◽  
Vol 27 (3) ◽  
pp. 357-364
Author(s):  
B. T. Chia ◽  
S.-A. Yang ◽  
M.-Y. Cheng ◽  
C.-W. Lin ◽  
Y.-J. Yang
Keyword(s):  
Polymerase Chain Reaction ◽  
Fluid Transport ◽  
Point Of Care ◽  
Reaction System ◽  
Thermal Characteristics ◽  
Pcr Amplification ◽  
Chain Reaction ◽  
Rapid Temperature ◽  
Small Footprint ◽  
Polymerase Chain

ABSTRACTIn this paper, the development of a portable polymerase chain reaction (PCR) device is presented. Integrating electromagnetic mini-actuators for bi-directional fluid transport, the proposed device, whose dimension is 67mm × 66mm × 25mm, can be fully operated with a 5V DC voltage. The device consists of four major parts: A disposable channel chip in which PCR mixture is manipulated and reacted, a heater chip which generates different temperature zones for PCR reaction, a linear actuator array for pumping PCR mixture, and a circuit module for controlling and driving the system. The advantages of the device include the rapid temperature responses associated with continuous-flow-type PCR devices, as well as the programmable thermal cycling associated with chamber-type PCR devices. The thermal characteristics are measured and discussed. PCR amplification is successfully performed for the 122 bp segment of MCF-7/adr cell line. Due to its small footprint, this self-contained system potentially can be employed for point-of-care (POC) applications.

scholarly journals Pemantauan Virus Dengan Metode PCR (Polymerase Chain Reaction) Di Pantai Utara Jawa Timur [Monitoring Virus By PCR Method (Polymerase Chain Reaction) In North Coast, East Java]

2019 ◽  
Vol 4 (1) ◽  
pp. 65
Author(s):  
Hari Suprapto, Yulia Kartika
Keyword(s):  
Water Quality ◽  
Polymerase Chain Reaction ◽  
White Spot Syndrome Virus ◽  
White Spot ◽  
Viral Diseases ◽  
Northern Coast ◽  
Taura Syndrome Virus ◽  
Chain Reaction ◽  
Yellow Head Virus ◽  
Polymerase Chain

Abstract The disease most dangerous for the cultivation activity is virus. Viruses are organisms subseluler that contain only nucleic acid (RNA or DNA) as genetic material. Koi Herpes Virus is one type of virus that causes mortality in cultured Cyprinids. KHV disease in Indonesia started in Blitar, East Java on March 2002 because the entry of imported koi fish that carry the virus KHV, while mortality prosentase could reach 80% - 85%, which causes loss of about 5 billion rupiah. In addition of KHV, there are several types of viral diseases in shrimp is White Spot Syndrome Virus (WSSV), Taura Syndrome Virus (TSV), dan Yellow Head Virus (YHV). Disease can cause losses in farming activities, such as WSSV. WSSV is an endemic disease since 1995. disease WSSV is exotic viral disease that attacks the shrimp monodon in 1998/1999 has resulted in decreased production of very large, so the Indonesian shrimp exports down 33,000 tons. Treatment of viral diseases is difficult because the virus resistant to certain antibiotics and chemical compounds. Therefore, prevention needs to be done, one through the monitoring activities conducted on the northern coast of East Java. The method implemented is monitoring in location and identification of viruses by PCR (Polymerase Chain Reaction). Monitoring in location includes water quality measurements and sampling. Identification of virus carried by IQ 2000TM. The identification procedure includes extraction, amplification and electrophoresis. Regional monitoring conducted on the northern coast of East Java includes Gresik, Lamongan, Tuban, Bangkalan, Sampang, Pamekasan, and Sumenep. Water quality at locations quite well. Results activities of monitoring on the northern coast of East Java is disease White Spot Syndrome Virus (WSSV) was found positive in several locations: Gresik, Lamongan and Tuban, while the virus Taura Syndrome Virus (TSV) and Yellow Head Virus (YHV) was not found at all locations . In tilapia, disease Koi Herpes Virus (KHV) was found positive in Tuban.

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