An integrated CMOS quantitative-polymerase-chain-reaction lab-on-chip for point-of-care diagnostics

Lab on a Chip ◽  
2014 ◽  
Vol 14 (20) ◽  
pp. 4076-4084 ◽  
Author(s):  
Haig Norian ◽  
Ryan M. Field ◽  
Ioannis Kymissis ◽  
Kenneth L. Shepard
Keyword(s):  
Integrated Circuit ◽  
Point Of Care ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Lab On Chip ◽  
Cmos Integrated Circuit ◽  
Point Of Care Diagnostics ◽  
Droplet Transport ◽  
Polymerase Chain ◽  
On Chip

qPCR demonstrated on the surface of a CMOS integrated circuit using embedded heaters, temperature sensors, photodiodes, and electrowetting-based droplet transport mechanism.

scholarly journals On-Chip PCR Based Plasmonic Microfluidic Platform: Ultrafast Point-of-Care Diagnostics of SARS-CoV-2

2021 ◽  
Vol 3 (1) ◽  
pp. 5-11
Author(s):  
Megha Agrawal ◽  
Keyword(s):  
Small Volume ◽  
Point Of Care ◽  
Rapid Screening ◽  
Viral Diseases ◽  
Chain Reaction ◽  
Point Of Care Diagnostics ◽  
Screening And Identification ◽  
Polymerase Chain ◽  
Diagnostic Applications ◽  
On Chip

It is critically important to have rapid screening and identification of contagious viral diseases such as the current COVID-19 pandemic that is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Rapid and accurate diagnostic is essential for preventing worldwide spread of virus and ensuring in-time care for patients during the fast spread of pandemic diseases. Nanobiotechnology enabled tools have allowed to develop advanced polymerase chain reaction (PCR) based diagnostics of contagious viral diseases. To this end, microfluidic on-chip PCR platforms have shown huge promise for highly efficient, rapid and small-volume bioassay for point-of-care (POC) diagnostic applications in mitigating the challenges of SARS-CoV-2. Here, we discuss latest advances in ultrafast, real-time, and on-chip nanoplasmonic PCR for rapid and quantitative molecular diagnostics at POC level.

scholarly journals Evaluation of commercially available immuno-magnetic agglutination and enzyme-linked immunosorbent assays for rapid point-of-care diagnostics of COVID-19

2020 ◽  
Author(s):  
Maria Engel Moeller ◽  
Jeppe Fock ◽  
Pearlyn Pah ◽  
Antia De La Campa Veras ◽  
Melanie Bade ◽  
...  
Keyword(s):  
Point Of Care ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plasma Samples ◽  
Igg Antibodies ◽  
Chain Reaction ◽  
Point Of Care Test ◽  
Point Of Care Diagnostics ◽  
Polymerase Chain ◽  
Respiratory Coronavirus ◽  
Immunomagnetic Assay

Introduction: Coronavirus Disease 2019 (COVID-19) is caused by severe acute respiratory coronavirus-2 (SARS-CoV-2). Fast, accurate and simple blood-based assays for quantification of anti-SARS-CoV-2 antibodies are urgently needed to identify infected individuals and keep track of the spread of disease. Methods: The study included 35 plasma samples from 22 individuals with confirmed COVID-19 by real time reverse transcriptase polymerase chain reaction and 40 non COVID-19 plasma samples. Anti-SARS-CoV-2 IgM/IgA or IgG antibodies were detected by a microfluidic quantitative immunomagnetic assay (IMA)(ViroTrack Sero COVID IgM+IgA/IgG Ab, Blusense Diagnostics, Denmark) and by enzyme-linked immunosorbent assay ((ELISA) (EuroImmun Medizinische Labordiagnostika, Germany). Results: Of the 35 plasma samples from the COVID-19 patients, 29 (82.9%) were positive for IgA/IgM or IgG by IMA and 29 samples (82.9%) were positive by ELISA. Sensitivity for only one sample per patient was 68% for IgA+IgM and 73% IgG by IMA and 73% by ELISA. For samples collected 14 days after symptom onset, the sensitivity of both IMA and ELISA was around 90%. Specificity of the IMA reached 100% compared to 95% for ELISA IgA and 97.5% for ELISA IgG. Conclusion: IMA for COVID-19 is a rapid simple-to-use point of care test with sensitivity and specificity similar to a commercial ELISA.

Plasmonic and label-free real-time quantitative PCR for point-of-care diagnostics

The Analyst ◽  
2021 ◽  
Author(s):  
Padideh Mohammadyousef ◽  
Miltiadis Paliouras ◽  
Mark Trifiro ◽  
Andrew Kirk
Keyword(s):  
Pathogen Detection ◽  
Point Of Care ◽  
Medical Community ◽  
Molecular Diagnostic ◽  
Label Free ◽  
Chain Reaction ◽  
Real Time Quantitative Pcr ◽  
Point Of Care Diagnostics ◽  
Polymerase Chain ◽  
Infectious Pathogen

In response to the world’s medical community need for accurate and immediate infectious pathogen detection, many researchers have focused on adapting the standard molecular diagnostic method of polymerase chain reaction...

scholarly journals Trends in photonic lab-on-chip interferometric biosensors for point-of-care diagnostics

2016 ◽  
Vol 8 (48) ◽  
pp. 8380-8394 ◽  
Author(s):  
A. B. González-Guerrero ◽  
J. Maldonado ◽  
S. Herranz ◽  
L. M. Lechuga
Keyword(s):  
Environmental Protection ◽  
Point Of Care ◽  
Lab On Chip ◽  
Universal Healthcare ◽  
Point Of Care Diagnostics ◽  
On Chip

Portable point-of care (POC) devices forin vitrodiagnostics will be a milestone for the achievement of universal healthcare and environmental protection.

AN IMPROVED METHOD FOR INHIBITOR FREE NUCLEIC ACIDS FROM POLYPHENOL RICH LEAVES OF MUSA SPP

2017 ◽  
Vol 23 (1) ◽  
Author(s):  
N.NANDHA KUMAR ◽  
K. SOURIANATHA SUNDARAM ◽  
D. SUDHAKAR ◽  
K.K. KUMAR
Keyword(s):  
Polymerase Chain Reaction ◽  
Quantitative Polymerase Chain Reaction ◽  
Proteinase K ◽  
Chain Reaction ◽  
Banana Plant ◽  
High Molecular Weight Dna ◽  
Musa Spp ◽  
Leaf Tissues ◽  
Polymerase Chain

Excessive presence of polysaccharides, polyphenol and secondary metabolites in banana plant affects the quality of DNA and it leads to difficult in isolating good quality of DNA. An optimized modified CTAB protocol for the isolation of high quality and quantity of DNA obtained from banana leaf tissues has been developed. In this protocol a slight increased salt (NaCl) concentration (2.0M) was used in the extraction buffer. Polyvinylpyrrolidone (PVP) and Octanol were used for the removal of polyphenols and polymerase chain reaction (PCR) inhibitors. Proteins like various enzymes were degraded by Proteinase K and removed by centrifugation from plant extract during the isolation process resulting in pure genomic DNA, ready to use in downstream applications including PCR, quantitative polymerase chain reaction (qPCR), ligation, restriction and sequencing. This protocol yielded a high molecular weight DNA isolated from polyphenols rich leaves of Musa spp which was free from contamination and colour. The average yields of total DNA from leaf ranged from 917.4 to 1860.9 ng/ìL. This modified CTAB protocol reported here is less time consuming 4-5h, reproducible and can be used for a broad spectrum of plant species which have polyphenol and polysaccharide compounds.

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