scholarly journals Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea

10.3791/56308 ◽  
2018 ◽  
Author(s):  
Rabab Sharif ◽  
Shrestha Priyadarsini ◽  
Tyler G. Rowsey ◽  
Jian-Xing Ma ◽  
Dimitrios Karamichos
Keyword(s):  
Tissue Engineering ◽  
Human Cornea ◽  
Corneal Tissue

scholarly journals Prospects and Challenges of Translational Corneal Bioprinting

2020 ◽  
Vol 7 (3) ◽  
pp. 71 ◽  
Author(s):  
Matthias Fuest ◽  
Gary Hin-Fai Yam ◽  
Jodhbir S. Mehta ◽  
Daniela F. Duarte Campos
Keyword(s):  
Tissue Engineering ◽  
Ex Vivo ◽  
Corneal Transplantation ◽  
Human Cornea ◽  
Corneal Tissue ◽  
Full Thickness ◽  
Future Directions ◽  
Spatial Control

Corneal transplantation remains the ultimate treatment option for advanced stromal and endothelial disorders. Corneal tissue engineering has gained increasing interest in recent years, as it can bypass many complications of conventional corneal transplantation. The human cornea is an ideal organ for tissue engineering, as it is avascular and immune-privileged. Mimicking the complex mechanical properties, the surface curvature, and stromal cytoarchitecure of the in vivo corneal tissue remains a great challenge for tissue engineering approaches. For this reason, automated biofabrication strategies, such as bioprinting, may offer additional spatial control during the manufacturing process to generate full-thickness cell-laden 3D corneal constructs. In this review, we discuss recent advances in bioprinting and biomaterials used for in vitro and ex vivo corneal tissue engineering, corneal cell-biomaterial interactions after bioprinting, and future directions of corneal bioprinting aiming at engineering a full-thickness human cornea in the lab.

scholarly journals Decellularized Human Stromal Lenticules Combine with Corneal Epithelial-Like Cells: A New Resource for Corneal Tissue Engineering

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Shuai Qin ◽  
Shuai Zheng ◽  
Bing Qi ◽  
Rui Guo ◽  
Guanghui Hou
Keyword(s):  
Tissue Engineering ◽  
Induction Medium ◽  
Nuclear Materials ◽  
Corneal Tissue ◽  
Corneal Epithelial ◽  
Human Ipscs ◽  
Immunological Rejection ◽  
Lenticule Extraction ◽  
Induced Pluripotent

The lack of donor corneal tissue or the immunological rejection remains a challenge for individuals with limbal stem cell deficiency (LSCD) who are treated with keratoplasty. Numerous lenticules which were extracted by small incision lenticule extraction (SMILE) appear to be useful materials for keratoplasty. In order to reduce the incidence of allograft rejection, lenticules would be decellularized. Lenticules which were treated with liquid nitrogen and nucleases had no cellular and nuclear materials remained. Human induced pluripotent stem cells (iPSCs) can be generated from the patient who requires keratoplasty, offering an autologous alternative and eliminating the risk of graft rejection. We found that BMP-4, RA, N-2 supplement, hEGF, B27, decellularized human stromal lenticules, conditioned medium, or induction medium promoted the differentiation of human iPSCs with high purity. The results showed that human iPSCs cultured for 4 days in differentiation medium A, 14 days in condition medium, and 1 week in induction medium on decellularized human stromal lenticules developed markedly higher expression of the markers P63, CK3, and CK12 than did those in the other methods. The level of gene expression of the epithelial and pluripotency markers and analysis by scanning electron microscopy and immunohistochemistry also showed successful differentiation. After inducing differentiation in vitro, corneal epithelial-like cells were induced. In the study, we investigated the possibility of a new resource for corneal tissue engineering.

scholarly journals Examining the Transmission of Visible Light through Electrospun Nanofibrous PCL Scaffolds for Corneal Tissue Engineering

Nanomaterials ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3191
Author(s):  
Marcus Himmler ◽  
Dirk W. Schubert ◽  
Thomas A. Fuchsluger
Keyword(s):  
Tissue Engineering ◽  
Visible Light ◽  
Light Transmission ◽  
Fiber Diameter ◽  
Surrounding Medium ◽  
Wound Dressings ◽  
Human Cornea ◽  
Corneal Tissue ◽  
Nanofibrous Scaffolds ◽  
Vis Spectroscopy

The transparency of nanofibrous scaffolds is of highest interest for potential applications like corneal wound dressings in corneal tissue engineering. In this study, we provide a detailed analysis of light transmission through electrospun polycaprolactone (PCL) scaffolds. PCL scaffolds were produced via electrospinning, with fiber diameters in the range from (35 ± 13) nm to (167 ± 35) nm. Light transmission measurements were conducted using UV–vis spectroscopy in the range of visible light and analyzed with respect to the influence of scaffold thickness, fiber diameter, and surrounding medium. Contour plots were compiled for a straightforward access to light transmission values for arbitrary scaffold thicknesses. Depending on the fiber diameter, transmission values between 15% and 75% were observed for scaffold thicknesses of 10 µm. With a decreasing fiber diameter, light transmission could be improved, as well as with matching refractive indices of fiber material and medium. For corneal tissue engineering, scaffolds should be designed as thin as possible and fabricated from polymers with a matching refractive index to that of the human cornea. Concerning fiber diameter, smaller fiber diameters should be favored for maximizing graft transparency. Finally, a novel, semi-empirical formulation of light transmission through nanofibrous scaffolds is presented.

scholarly journals Biomaterials for corneal endothelial cell culture and tissue engineering

2021 ◽  
Vol 12 ◽  
pp. 204173142199053
Author(s):  
Mohit Parekh ◽  
Vito Romano ◽  
Kareem Hassanin ◽  
Valeria Testa ◽  
Rintra Wongvisavavit ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cells ◽  
Standard Treatment ◽  
Corneal Endothelial Cell ◽  
Corneal Tissue ◽  
Corneal Endothelial Cells ◽  
Endothelial Cell Culture ◽  
Corneal Transplants

The corneal endothelium is the posterior monolayer of cells that are responsible for maintaining overall transparency of the avascular corneal tissue via pump function. These cells are non-regenerative in vivo and therefore, approximately 40% of corneal transplants undertaken worldwide are a result of damage or dysfunction of endothelial cells. The number of available corneal donor tissues is limited worldwide, hence, cultivation of human corneal endothelial cells (hCECs) in vitro has been attempted in order to produce tissue engineered corneal endothelial grafts. Researchers have attempted to recreate the current gold standard treatment of replacing the endothelial layer with accompanying Descemet’s membrane or a small portion of stroma as support with tissue engineering strategies using various substrates of both biologically derived and synthetic origin. Here we review the potential biomaterials that are currently in development to support the transplantation of a cultured monolayer of hCECs.

Reconstructed Human Cornea Produced in vitro by Tissue Engineering

Pathobiology ◽  
1999 ◽  
Vol 67 (3) ◽  
pp. 140-147 ◽  
Author(s):  
Lucie Germain ◽  
François A. Auger ◽  
Eric Grandbois ◽  
Rina Guignard ◽  
Marcelle Giasson ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Human Cornea

Decellularized bone extracellular matrix in skeletal tissue engineering

2020 ◽  
Vol 48 (3) ◽  
pp. 755-764
Author(s):  
Benjamin B. Rothrauff ◽  
Rocky S. Tuan
Keyword(s):  
Tissue Engineering ◽  
Bone Regeneration ◽  
Tissue Regeneration ◽  
Animal Studies ◽  
Tumor Resection ◽  
Regenerative Capacity ◽  
Skeletal Tissue ◽  
Large Bone

Bone possesses an intrinsic regenerative capacity, which can be compromised by aging, disease, trauma, and iatrogenesis (e.g. tumor resection, pharmacological). At present, autografts and allografts are the principal biological treatments available to replace large bone segments, but both entail several limitations that reduce wider use and consistent success. The use of decellularized extracellular matrices (ECM), often derived from xenogeneic sources, has been shown to favorably influence the immune response to injury and promote site-appropriate tissue regeneration. Decellularized bone ECM (dbECM), utilized in several forms — whole organ, particles, hydrogels — has shown promise in both in vitro and in vivo animal studies to promote osteogenic differentiation of stem/progenitor cells and enhance bone regeneration. However, dbECM has yet to be investigated in clinical studies, which are needed to determine the relative efficacy of this emerging biomaterial as compared with established treatments. This mini-review highlights the recent exploration of dbECM as a biomaterial for skeletal tissue engineering and considers modifications on its future use to more consistently promote bone regeneration.

Bioreaktoren für Knochen-Tissue-Engineering

2013 ◽  
Vol 22 (03) ◽  
pp. 188-195 ◽  
Author(s):  
H.-H. Hsu ◽  
C. Goepfert ◽  
R. Pörtner
Keyword(s):  
Tissue Engineering

ZusammenfassungZur medizinischen Behandlung großer Knochendefekte oder Verletzungen werden als Alternative zu etablierten Behandlungs-methoden neue Konzepte des Tissue Engineering (TE) diskutiert. Beim Knochen-TE ist es das Ziel, eine mit Zellen besiedelte drei-dimensionale (3D), biologisch abbaubare Struktur am Ort der Verletzung zu implantieren. Techniken für die organotypische Kultivierung von Knochenzellen in vitro beruhen auf der Kultivierung von Gewebezellen in Bioreaktoren in einem definierten Kultur-medium auf porösen Matrizes (Scaffolds), um ein gewebeähnliches Wachstum in 3D-Strukturen zu ermöglichen. Ein wichtiger Faktor für die erfolgreiche 3D-Kultur ist die Schaffung adäquater Strömungsbedingungen, die wiederum Einfluss auf die biochemischen und biophysikalischen (z. B. mechanische) Reize haben, denen die Zellen ausgesetzt sind. Hier müssen neben Schereffekten auch Stofftransportlimitierungen berücksichtigt werden. Der Beitrag fasst den aktuellen Stand bei der Entwicklung von Bioreaktoren für die Generierung von Knochenersatzmaterialien zusammen.

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